Recombinant Streptomyces netropsis transglutaminase expressed in Komagataella phaffii (Pichia pastoris) and applied in plant-based chicken nugget.

Transglutaminase (TG, EC 2.3.2.13) is widely used to modify functional properties in food systems, which can catalyze cross-linking reaction of proteins. In this work, microbial transglutaminase (MTG) from Streptomyces netropsis was heterologously expressed in the methylotrophic yeast Komagataella phaffii (Pichia pastoris). The specific activity of recombinant microbial transglutaminase (RMTG) was 26.17 ± 1.26 U/mg, and the optimum pH and temperature were measured as 7.0 and 50 °C, respectively. Bovine serum albumin (BSA) was used as a substrate to evaluate the effect of cross-linking reaction, and we found that RMTG had significant (p < 0.05) cross-linking effect for more than 30 min reactions. RMTG was further utilized in the investigation of plant-based chicken nuggets. Results showed that the hardness, springiness and chewiness of nuggets increased, and the adhesiveness decreased after RMTG treatment, which can prove that RMTG has the potential to improve the texture properties of plant-based chicken nuggets.

Inactivation of norovirus by atmospheric pressure plasma jet on salmon sashimi.

This study investigated atmospheric pressure plasma (APP) jet, an emerging novel non-thermal technology, for the inactivation of human norovirus (NoV) on salmon sashimi. The influences of the non-thermal plasma on quality attributes of sashimi were also evaluated. Air, O2, and N2 (15 L/min) were used to produce the plasma jets. N2 plasma treatment for 12 min reduced NoV viral load (VL) (initial inoculums of 2.7 × 104 copies/g) by 2.17 × 104 copies/g, while air-based or O2-based plasma treatment for 9-12 min could reduce the VL to undetectable levels (below 100 copies/g). Under the same operating condition, the air-based or O2-based plasma treatment might increase slightly TBARS values in sashimi, yet the values (far below 1.0 mg MDA/kg) were within acceptable level for sashimi made with salmon fishes. The APP jets (APPJ) treatments could also retain the pH of sashimi at normal levels (6.29 ~ 6.02) to maintain the quality of salmon sashimi, the color quality of which was not affected evidently. The plasma-induced hardness and springiness changes in salmon sashimi were substantially low. These results suggested APPJ could be implemented as technology for inactivation of food-borne viruses and exhibited a high potential for application in fish sashimi processing, retaining product quality as well.

Non-thermal atmospheric gas plasma for decontamination of sliced cheese and changes in quality.

The atmospheric-pressure non-thermal dielectric barrier discharge (DBD) plasma has recently emerged as an efficient decontamination method for the food safety enhancement. Thus the objective of this study was to evaluate the effectiveness of a simple DBD plasma treatment, with a relatively low-frequency power supply operating at 60 Hz, for microbial inactivation. A parametric study of operating conditions for bacterial inactivation was conducted using nutrient agar inoculated with Escherichia coli (2.28--6.28 log CFU/ml). The microbial log reduction was enhanced with increasing input power (30, 50, 70 W) and plasma exposure time (0, 1, 3, 5, 7 min). The inactivation effect was increased by decreasing inter-electrode gap (2, 1.5, 1 cm) and by reducing the initial microorganism concentration. Accordingly, a DBD plasma treatment at 50 W for 10 min could lead to complete killing of E. coli and partial inactivation of Listeria innocua on cheese (mean log reduction: 4.75 ± 0.02 and 0.72 ± 0.01, respectively). The decontamination efficacy of DBD plasma was affected by the types of microorganisms. The changes in hardness and color of cheese were unnoticeable after 10 min treatment with a power of 50 W. Overall, the results suggested that the DBD plasma can be potentially exploited to improve the food safety.