[Cloning of Chrysanthium stund virion cDNA in pUC19 plasmid and the use of cloned cDNA for detecting the virion]. / Klonirovanie kDNK viroida karlikovosti khrizantem v plazmide pUC19 i ispol'zovanie klonirovannoi kDNK dliia obnaruzheniia viroida karlikovosti khrizantem.

26 base long deoxyribonucleotide complementary to the lower part of the Central Conserved Region of chrysanthemum stund viroid (CSV) was used for synthesis of the first strand cDNA. The cDNA was cloned into plasmid vector pUC19 and the primary structure was determined. Cloned, full length cDNA was used as hybridisation probe for detection of CSV. It was possible to detect about 26 pg of purified CSV RNA immobilized on nitrocellulose filters using 32P-labeled probe. In the case of biotinylated probe it was possible to detect about 26 pg of purified CSV RNA visualizing results by streptavidin-alkaline phosphatase conjugates. It has been shown that such a cloned cDNA can be used for wide scale detection of CSV.

[Primary structure of the 3'-terminal region of the cloned DNA of the bovine leukemia virus]. / Pervichnaia struktura 3'-kontsevoi oblasti klonirovaniia kDNK virusa bych'ego leikoza.

The nucleotide sequence of the 3'-terminal region of the cloned bovine leukaemia virus cDNA (1474 bp) was elucidated using both Sanger and Maxam-Gilbert techniques. This DNA region contains U3 and R parts of the BLV LTR and an upstream sequence with four open reading frames (ORF) of unknown function. The comparison of the nucleotide substitutions in these ORF with the two variants of proviral BLV DNA suggests that the only pX1 ORF possesses a coding function. The role of the pX1 protein is discussed.

[The current methods for the prevention, diagnosis and surgical treatment of the adhesive complications of peritonitis in children]. / Sovremennye metody profilaktiki, diagnostiki i khirurgicheskogo lecheniia spaechnykh oslozhnenii peritonita u detei.

The paper is based on clinical and experimental findings of one of the postoperative complications of purulent peritonitis, namely adhesion formation. The authors consider that impaired cytodynamic relationship in the focus of peritonitis concurrent with gastrointestinal paresis underlie the pathogenesis of the above-mentioned complication. In this connection, the authors propose a set of anti-adhesive measures, which involves prodigiosane cytomodulation and differential treatment of enteroparesis. Intestinal ultrasonic echocardiography was used for diagnostic purposes. Laparoscopy was employed in the surgical treatment of adhesive complications of peritonitis, which allowed relaparotomy to be eliminated or its traumaticity to be reduced to a great extent. There were no deaths and relapses of adhesive disease.

[Surgical treatment of appendicular peritonitis in children]. / Khirurgicheskoe lechenie appendikuliarnogo peritonita u detei.

The article is based on the results of 10-year use of the method of aspiration drainage of the abdominal cavity in the treatment of 980 children with purulent peritonitis of appendicular origin. The authors used only a polymer tube to drain the cavity of the true pelvis and in principle did not resort to intraoperative irrigation of the abdominal cavity, postoperative lavage, and intraperitoneal injection of antibiotics. Postoperative complications were recognized in the early stages of development and were treated by nonoperative methods on the main. The number of relaparotomies was 0.83% of the total number of operations for appendectomy. The described method meets the principles of pediatric surgery most fully, is more sparing than the other methods, and the authors recommend it for wide introduction into practice.

[Prophylaxis, diagnosis and surgical treatment of adhesions in the abdominal cavity of children]. / Profilaktika, diagnostika i khirurgicheskoe lechenie spaechnogo protsessa briushnoi polosti u detei.

The clinical and experimental study of postoperative complications of suppurative peritonitis (the pathologic commissure formation) has been done. The authors believe that the combination of the break of cytodynamic correlation in peritonitis centre and GIT paresis is a basis of pathogenesis of the process. The authors advocate combination of anticomissural preventive stern with cytomodulation and GIT paresis treatment. The abdominal ultrasonography has been used for the diagnosis. The main role in surgical treatment belongs to laparoscopy that made it possible to decrease the amount of relaparotomy cases. There were no lethal complications or cases of recurrency.

[Ultrasonic diagnosis of limited forms of appendicular peritonitis in children]. / Ekhografiia v diagnostike otgranichennykh form appendikuliarnogo peritonita u detei.

Results of an analysis of echographic diagnostics of appendicular peritonitis in 60 children have shown its high informative value. Under consideration are problems of the rational technique of investigation depending on localization of the pathological focus. Differential diagnostic criteria of various forms of limited peritonitis in children are presented. Possibilities of the method to establish the objective efficiency of the therapeutic treatment and substantiation of tactics of the treatment are shown.

[Diagnosis of septic conditions in newborn infants with surgical diseases]. / Diagnostika septicheskikh sostoianii u novorozhdennykh s khirurgicheskimi zabolevaniiami.

An analysis of 65 case histories of newborns with pyo-septic surgical diseases has been made. The extraction-spectrometrical method of analysis of blood serum is proposed. The distribution index (DI) in sepsis was always less than 1. Local forms of purulent infection were diagnosed if the DI was more than 1.