Recent Advances in the Development of Sustainable Composite Materials used as Membranes in Microbial Fuel Cells.

MFC can have dual functions; they can generate electricity from industrial and domestic effluents while purifying wastewater. Most MFC designs comprise a membrane which physically separates the cathode and anode compartments while keeping them electrically connected, playing a significant role in its efficiency. Popular commercial membranes such as Nafion, Hyflon and Zifron have excellent ionic conductivity, but have several drawbacks, mainly their prohibitive cost and non-biodegradability, preventing the large-scale application of MFC. Fabrication of composite materials that can function better at a much lower cost while also being environment-friendly has been the endeavor of few researchers over the past years. The current review aims to apprise readers of the latest trends of the past decade in fabricating composite membranes (CM) for MFC. For emphasis on environmental-friendly CM, the review begins with biopolymers, moving on to the carbon-polymer, polymer-polymer, and metal-polymer CM. Lastly, critical analysis towards technology-oriented propositions and realistic future directives in terms of strengths, weakness, opportunities, challenges (SWOC analysis) of the application of CM in MFC have been discussed for their possible large-scale use. The focus of this review is the development of hybrid materials as membranes for fuel cells, while underscoring the need for environment-friendly composites and processes.

Systematic computational toxicity analysis of the ozonolytic degraded compounds of azo dyes: Quantitative structure-activity relationship (QSAR) and adverse outcome pathway (AOP) based approach.

The present study identifies and analyses the degraded products of three azo dyes (Reactive Orange 16, Reactive Red 120, and Direct Red 80) and proffers their in silico toxicity predictions. In our previously published work, the synthetic dye effluents were degraded using an ozonolysis-based Advanced Oxidation Process. In the present study, the degraded products of the three dyes were analysed using GC-MS at endpoint strategy and further subjected to in silico toxicity analysis using Toxicity Estimation Software Tool (TEST), Prediction Of TOXicity of chemicals (ProTox-II), and Estimation Programs Interface Suite (EPI Suite). Several physiological toxicity endpoints, such as hepatotoxicity, carcinogenicity, mutagenicity, cellular and molecular interactions, were considered to assess the Quantitative Structure-Activity Relationships (QSAR) and adverse outcome pathways. The environmental fate of the by-products in terms of their biodegradability and possible bioaccumulation was also assessed. Results of ProTox-II suggested that the azo dye degradation products are carcinogenic, immunotoxic, and cytotoxic and displayed toxicity towards Androgen Receptor and Mitochondrial Membrane Potential. TEST results predicted LC50 and IGC50 values for three organisms Tetrahymena pyriformis, Daphnia magna, and Pimephales promelas. EPISUITE software via the BCFBAF module surmises that the degradation products' bioaccumulation (BAF) and bioconcentration factors (BCF) are high. The cumulative inference of the results suggests that most degradation by-products are toxic and need further remediation strategies. The study aims to complement existing tests to predict toxicity and prioritise the elimination/reduction of harmful degradation products of primary treatment procedures. The novelty of this study is that it streamlines in silico approaches to predict the nature of toxicity of degradation by-products of toxic industrial affluents like azo dyes. These approaches can assist the first phase of toxicology assessments for any pollutant for regulatory decision-making bodies to chalk out appropriate action plans for their remediation.

Ceramic-microbial fuel cell (C-MFC) for waste water treatment: A mini review.

Microbial fuel cell (MFC) is a bio-electrochemical system that utilizes the activity of electrogenic bacteria to generate electricity. When wastewater is used as feed in MFC, its organic constituents are hydrolyzed and oxidized by the bacteria. Hence, this technology is a source of clean electricity while simultaneously treating wastewater. Over the years much research has been done to improve its efficiency as well as to reduce the cost of implementation and functioning. However, scalability and commercialization of this technology still faces several challenges. This mini review discusses the use of ceramics in MFCs using wastewater feed as a method of overcoming the current technological challenges. Ceramics can be used as separators, chassis or electrode, conferring facile chemical and structural stability. The material is low-cost, environment-friendly and easily available. Studies reporting stacked configurations have been mentioned, and those that have reported field studies and technology oriented practical applications. Critical analysis of the scalability of the use of ceramics for the dual purpose of electricity generation as well as wastewater treatment has been done in this review. Future research directives towards potential sustainable commercialization have also been mentioned. C-MFC is a promising technology and the primary aim of this review is to help enhance the knowledge base for the optimization of use of ceramics in MFC to achieve large-scale clean electricity generation and sewage treatment.

Bioaerogels, the emerging technology for wastewater treatment: A comprehensive review on synthesis, properties and applications.

Over the past decade use of aerogels has received much attention as an emerging technology for wastewater treatment. However, production of aerogels is not environment-friendly. Owing to its excellent properties such as porosity, three-dimensional structure, being amenable to chemical modifications, it is imperative to devise strategies for their improved production and use. Bioaerogels are non-toxic and most of their precursor compounds are biomass-derived. This review aims to present a comprehensive report on survey of existing literature published on the use of bioaerogels for removal of all major categories of water contaminants, namely, heavy metals, industrial dyes, oil, organic compounds and pharmaceuticals. It also gives critical analysis of the lacunae in the existing knowledge such as lack of studies on domestic sewage, emerging pollutants, toxicity of raw materials and adequate disposal of used adsorbents. Proposals of overcoming the limitations in the applicability of bioaerogels, like combining constructed wetlands with use of bioaerogels, among others have been discussed. In this review, emphasis has been given on production of bioaerogels, with an aim to underscore the potential of valorization of biomass waste to develop novel materials for wastewater treatment in an effort towards creating a circular and green economy.

Atrazine detoxification by intracellular crude enzyme extracts derived from epiphytic root bacteria associated with emergent hydrophytes.

The present study demonstrated atrazine detoxification by intracellular crude enzyme extracts of Pseudomonas spp. strains ACB and TLB. Indigenous bacterial protein-based remediation techniques could be an alternative to bioaugmentation which pose multiple challenges when applied to the field. Intracellular enzymes were extracted from strains ACB and TLB and their degradation potential of 10 mg L-1 was determined using Gas Chromatography; further, enzyme extracts were subjected to protein profiling studies. In span of 6 h, enzyme extracts of strain ACB showed maximum degradation at 30 °C and 40 °C (71%) and enzyme extracts of strain TLB showed maximum degradation at 40 °C (48%). Atrazine degradation by enzyme extracts of strain ACB showed maximum degradation at pH 7 (71%) and pH 6 (69%) in 6 h. Similarly, enzyme extracts of strain TLB showed maximal degradation at pH 6 (46%) in 6 h. The present study demonstrated, for the first time, efficient atrazine remediation by intracellular crude enzyme extracts from epiphytic root bacteria at a range of temperature and pH conditions. Protein profiling studies indicated that atrazine induced expression of CoA ester lyase and alkyl hydroperoxide reductase in the strains ACB and TLB respectively. Expressions of these proteins have never been associated with atrazine exposure.

Enhanced atrazine removal by hydrophyte-bacterium associations and in vitro screening of the isolates for their plant growth-promoting potential.

Emergent hydrophytes Acorus calamus, Typha latifolia, and Phragmites karka and epiphytic root bacteria isolated from their rhizoplanes were exposed to atrazine (5 and 10 mg l-1) individually and in plant-bacterium combination for 15 days hydroponically. It was observed that A. calamus-Pseudomonas sp. strain, the ACB combination, was best in decontamination, showing 91% and 87% removal of 5 and 10 mg l-1 atrazine. Plant-bacterium association led to significant increase in atrazine decontamination as compared to decontamination by either plant or bacterium alone, indicating a synergistic action of the hydrophytes and isolates which led to enhanced atrazine removal. To the best of our knowledge this is the first report on the potential of plant-bacterium combinations for atrazine decontamination. The isolates showed augmented growth in the presence of plants and were able to alleviate atrazine stress in them. These isolates exhibited plant growth-promoting traits such as auxin, siderophore, Poly(3-hydroxybutyric acid)/succinogycan, ammonia, catalase production and solubilization of inorganic phosphate in vitro. The use of plant-bacterium mutualistic symbiosis for atrazine mitigation is a relatively simple, inexpensive, and clean technique and this phytoremediation-rhizoremediation combination is suggested to be tried on field to establish their potential for clean-up of contaminated sites.

Advances in amelioration of air pollution using plants and associated microbes: An outlook on phytoremediation and other plant-based technologies.

Globally, air pollution is an unfortunate aftermath of rapid industrialization and urbanization. Although the best strategy is to prevent air pollution, it is not always feasible. This makes it imperative to devise and implement techniques that can clean the air continuously. Plants and microbes have a natural potential to transform or degrade pollutants. Hence, strategies that use this potential of living biomass to remediate air pollution seem to be promising. The simplest future trend can be planting suitable plant-microbe species capable of removing air pollutants like SO2, CO2, CO, NOX and particulate matter (PM) along roadsides and inside the buildings. Established wastewater treatment strategies such as microbial fuel cells (MFC) and constructed wetlands (CW) can be suitably modified to ameliorate air pollution. Green architecture involving green walls and green roofs is facile and aesthetic, providing urban ecosystem services. Certain microbe-based bioreactors such as bioscrubbers and biofilters may be useful in small confined spaces. Several generative models have been developed to assist with planning and managing green spaces in urban locales. The physiological limitations of using living organisms can be circumvent by applying biotechnology and transgenics to improve their potential. This review provides a comprehensive update on not just the plants and associated microbes for the mitigation of air pollution, but also lists the technologies that are available and/or can be modified and used for air pollution control. The article also gives a detailed analysis of this topic in the form of strengths-weaknesses-opportunities-challenges (SWOC). The strategies mentioned in this review would help to attain corporate Environmental Social and Governance (ESG) and Sustainable Development Goals (SDGs), while reducing carbon footprint in the urban scenario. The review aims to emphasise that urbanization is possible while tackling air pollution using facile, green techniques involving plants and associated microbes.