RESUMEN
Cases of sarcoptic mange in the wildlife population have been increasing around the world in recent years. In this study, we report the first case of severe sarcoptic mange in two Andean foxes (Lycalopex culpaeus) and molecularly analyze the collected mites. The National Forestry and Wildlife Service of Lima, Peru, found an adult male Andean fox in the province of Callao in August 2022. The veterinarians decided to euthanize the fox due to the severity of the mange. In August 2023, an adult male Andean fox was found dead in the province of Huaral by veterinarians. Both foxes were sent to the veterinary school in Lima for necropsy. Skin samples from different body zones were digested in buffer lysis, and mites were detected in the tissue samples. A morphological diagnosis identified the mites as Sarcoptes scabiei. The mites from both foxes had the same nuclear (ITS2) and mitochondrial (cox1) genetic marker sequences as the S. scabiei sequences from dogs, vicunas, Andean foxes, and water buffalos recorded in GenBank. Unlike ITS2, phylogenetic analysis of S. scabiei cox1 showed host-related nucleotide sequence polymorphisms. Future molecular studies of S. scabiei from different hosts and localities will be necessary to better understand the transmission of this disease in Andean foxes.
Asunto(s)
Zorros , Escabiosis , Animales , Masculino , Animales Salvajes , Perú , Filogenia , Sarcoptes scabiei/genética , Escabiosis/veterinariaRESUMEN
Hydatigera taeniaeformis is a cestode that uses felines and rodents as definitive and intermediate hosts, respectively. Its larval stage, or metacestode, infects a wide variety of rodent species and develops in the liver parenchyma into a cyst. The aim of this study was to evaluate the occurrence of H. taeniaeformis metacestode in various species of wild rodents from Peru. For this, the livers of 356 rodents were macroscopically examined for any parasitic form compatible with metacestodes. Metacestodes were identified by measuring characteristic morphological parameters, and the diagnosis was confirmed by molecular analysis of a fragment of the cytochrome c oxidase subunit 1 gene (cox1). Five rodents: two small-eared pygmy rice rats (Oligoryzomys microtis), two white-naped squirrels (Simosciurus nebouxii), and one pygmy rice rat (Oligoryzomys sp.) were infected with H. taeniaeformis metacestodes. The cox1 sequences from our metacestodes showed up to 100% identity with previous H. taeniaeformis sequences from the GenBank. These results demonstrated the occurrence of H. taeniaeformis in new intermediate hosts, as well as the first molecular contribution for H. taeniaeformis from Peru.
Asunto(s)
Cestodos , Taenia , Ratas , Gatos , Animales , Perú/epidemiología , Taenia/genética , Cestodos/genética , Cestodos/anatomía & histología , Sciuridae , Larva , SigmodontinaeRESUMEN
La neosporosis es una parasitosis de importancia en el sector ganadero por ser causante de abortos y mortalidad neonatal. El objetivo del estudio fue determinar la presencia de anticuerpos contra Neospora caninum en búfalos de agua procedentes de cinco centros de crianza ubicados en el distrito Jenaro Herrera, departamento de Loreto, Perú, en el año 2008. Se evaluaron 83 muestras de suero, obtenidas de búfalas en edad productiva, criadas para la producción de leche. Las muestras se evaluaron por ELISA indirecto e inmunofluorescencia indirecta (IFI). No se halló evidencia de anticuerpos contra N. caninuma las diluciones 1:25 y 1:100 utilizando ELISA indirecto, así como a la dilución 1:100 con IFI. Los resultados sugieren una baja o nula exposición de los búfalos de agua al parásito en la zona estudiad.
Neosporosis is an important parasitic disease that causes reproductive problems as abortion and neonatal mortality in diary cattle. The aim of the study was to determine the presence of antibodies against Neospora caninumin water buffaloes reared in five farms located in Jenaro Herrera district, Loreto, Peru in 2008. Blood samples were collected from 83 female dairy buffalo older than 2 years. Serum samples were tested by indirect ELISA and immunnefluorescent antibody test (IFAT). None of sera presented antibodies against N. caninum to the dilution 1:25 and 1:100 using indirect ELISA or to the dilution 1:100 by IFAT. The results suggest a low exposition of water buffaloes to N. caninum in the studied area.