RESUMEN
Friedreich Ataxia (FRDA), the most common inherited ataxia, arises from defective expression of the mitochondrial protein frataxin, which leads to increased mitochondrial oxidative damage. Therefore, antioxidants targeted to mitochondria should be particularly effective at slowing disease progression. To test this hypothesis, we compared the efficacy of mitochondria-targeted and untargeted antioxidants derived from coenzyme Q10 and from vitamin E at preventing cell death due to endogenous oxidative stress in cultured fibroblasts from FRDA patients in which glutathione synthesis was blocked. The mitochondria-targeted antioxidant MitoQ was several hundredfold more potent than the untargeted analog idebenone. The mitochondria-targeted antioxidant MitoVit E was 350-fold more potent than the water soluble analog Trolox. This is the first demonstration that mitochondria-targeted antioxidants prevent cell death that arises in response to endogenous oxidative damage. Targeted antioxidants may have therapeutic potential in FRDA and in other disorders involving mitochondrial oxidative damage.
Asunto(s)
Antioxidantes/farmacología , Ataxia de Friedreich/metabolismo , Mitocondrias/efectos de los fármacos , Estrés Oxidativo , Ubiquinona/análogos & derivados , Antioxidantes/química , Benzoquinonas/química , Benzoquinonas/farmacología , Muerte Celular , Sistemas de Liberación de Medicamentos , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Ataxia de Friedreich/patología , Humanos , Modelos Biológicos , Compuestos Organofosforados/química , Compuestos Organofosforados/farmacología , Ubiquinona/química , Ubiquinona/farmacología , Vitamina E/análogos & derivadosRESUMEN
Friedreich Ataxia (FRDA), the most frequent inherited ataxia, is not only characterized by progressive gait and limb ataxia, but in most cases is also accompanied by a severe hypertrophic cardiomyopathy. This life threatening symptom can be ameliorated by the administration of idebenone, a short chain quinone antioxidant, supporting additional evidence that oxidative stress plays a major role in the pathogenesis of this disease. In this study we analyze the combinatorial effect of different antioxidants on cell viability of FRDA fibroblasts and of RAT-1 immortalized fibroblasts exposed to oxidative stress. We find that an equimolar mixture of idebenone and vitamin E is more potent than each of the compound alone. Increased potency was also obtained with a novel synthetic antioxidant (Fe-Aox29) combining the active groups from both idebenone and vitamin E. These results indicate, that idebenone and vitamin E might act synergistically to counteract oxidative stress in fibroblasts from FRDA patients.
Asunto(s)
Antioxidantes/farmacología , Benzoquinonas/farmacología , Cromanos/farmacología , Fibroblastos/efectos de los fármacos , Ataxia de Friedreich/patología , Sustancias Protectoras/farmacología , Vitamina E/farmacología , Animales , Antioxidantes/química , Benzoquinonas/química , Línea Celular Transformada , Supervivencia Celular/efectos de los fármacos , Cromanos/química , Fibroblastos/patología , Humanos , Peróxido de Hidrógeno/farmacología , Sustancias Protectoras/química , Ratas , Especies Reactivas de Oxígeno/metabolismo , Ubiquinona/análogos & derivados , Vitamina E/químicaRESUMEN
Friedreich Ataxia (FRDA), the most prevalent of the inherited ataxias, is a multi-systemic disease with loss of sensory neurons and life-threatening hypertrophic cardiomyopathy as its most severe manifestations. Reduced levels of the mitochondrial protein frataxin lead to cell-damaging oxidative stress and consequently FRDA is considered as a model for more common neurodegenerative disorders in which reactive radicals and oxidative stress are involved. We have developed a cellular assay system that discriminates between fibroblasts from FRDA patients and unaffected donors on the basis of their sensitivity to pharmacological inhibition of de novo synthesis of glutathione. With this assay we observed that supplementation with selenium effectively improved the viability of FRDA fibroblasts, indicating that basal selenium concentrations are not sufficient to allow an adequate increase in the activity of certain detoxification enzymes (such as GPX). Furthermore, we characterized potential drug candidates and found that idebenone, a mitochondrially localized antioxidant that ameliorates cardiomyopathy in FRDA patients, as well as other lipophilic antioxidants protected FRDA cells from cell death. Our results also demonstrate for the first time that small-molecule GPX mimetics have potential as a novel treatment strategy for Friedreich Ataxia and presumably also for other neurodegenerative diseases with mitochondrial impairment.