Activation of aryl hydrocarbon receptor ameliorates rosacea-like eruptions in mice and suppresses the TLR signaling pathway in LL-37-induced HaCaT cells.

BACKGROUND: Toll-like receptor (TLR) 2, along with some chemokines, were found to be overexpressed in rosacea patients. Aryl hydrocarbon Receptor (AhR) activation inhibited the inflammatory responses triggered by TLR activation. The current study was conducted to evaluate the underlying mechanisms of AhR activation in rosacea models. MATERIALS AND METHODS: Seven-week-old female BALB/c mice received twice daily intradermal injections of LL-37 for 2 consecutive days. Thirty minutes after the second LL-37 injection, 1% or 0.5% AhR agonist benvitimod was administrated topically once per day for 3 consecutive days. HaCaT cells were treated with different concentrations of LL-37 and benvitimod, and were further infected with lentivirus to over-express TLR2. Expressions of TLR2, CCL5, CXCL9, CXCL10 and CXCL11 were evaluated using qRT-PCR, Western Blot or ELISA. RESULTS: AhR activation ameliorated LL-37-induced rosacea-like eruptions in mice by reductions in redness scores, redness areas and dermal inflammatory cell infiltrates. Elevated expressions of TLR2 and chemokines (CCL5, CXCL9, CXCL10 and CXCL11) following LL-37 treatment were decreased by AhR activation. In HaCaT cells receiving LL-37, TLR2 and the four chemokines were up-regulated, and levels of these chemokines were further enhanced after over-expressing TLR2. At 8 h after an administration of 10 µM benvitimod, gene expressions of TLR2 and the four chemokines in LL-37 treated HaCat cells were decreased, while their protein expressions were decreased for 24 h. CONCLUSION: AhR activation is beneficial in treating rosacea in a LL-37-induced rosacea mouse model and involves a suppression of the TLR signaling pathway in an HaCaT cell model of rosacea.

High power picosecond green and deep ultraviolet generations with an all-fiberized MOPA.

We demonstrate high power picosecond green and deep ultraviolet (DUV) lasers based on an all-fiberized master oscillator power amplifier (MOPA). The main power amplifier is fabricated with a highly Yb-doped large mode area (LMA) silicate glass fiber. It delivers 75.2-W laser output at 1029 nm with a pulse repetition rate of 10 MHz and a pulse duration of 70 ps. With a lithium triborate (LBO) crystal, a 43.0-W green output at 514.5 nm has been achieved with a pulse duration of 55 ps. With a caesium lithium borate (CLBO) crystal, a 14.5-W picosecond DUV output at 257 nm has been generated, which is the highest power for the all-fiber based DUV laser, to the best of our knowledge.

Single frequency tunable UV laser at 308†nm based on all-fiberized master oscillator power amplifiers.

A tunable narrow linewidth UV laser near 308 nm is necessary for highly sensitive hydroxyl (OH) radical measurement. We demonstrated a high-power fiber-based single frequency tunable pulsed UV laser at 308 nm. The UV output is generated from the sum frequency of a 515 nm fiber laser and a 768 nm fiber laser, which are harmonic generations from our proprietary high-peak-power silicate glass Yb- and Er-doped fiber amplifiers. A 3.50 W single frequency UV laser with 100.8 kHz pulse repetition rate, 3.6 ns pulse width, 34.7 µJ pulse energy, and 9.6 kW peak power has been achieved, which represents the first demonstration, to the best of our knowledge, of a high-power fiber-based 308 nm UV laser. With temperature control of the single frequency distributed feedback seed laser, the UV output is tunable for up to 792 GHz at 308 nm.

UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.

Both SIRT1 and UVA radiation are involved in cellular damage processes such as apoptosis, senescence and ageing. MicroRNAs (miRNAs) have been reported to be closely related to UV radiation, as well as to SIRT1. In this study, we investigated the connections among SIRT1, UVA and miRNA in human skin primary fibroblasts. Our results showed that UVA altered the protein level of SIRT1 in a time point-dependent manner. Using miRNA microarray, bioinformatics analysis, we found that knocking down SIRT1 could cause up-regulation of miR-27a-5p and the latter could down-regulate SMAD2, and these results were verified by qRT-PCR or Western blot. Furthermore, UVA radiation (5 J/cm2 ), knocking down SIRT1 or overexpression of miR-27a-5p led to increased expression of MMP1, and decreased expressions of COL1 and BCL2. We also found additive impacts on MMP1, COL1 and BCL2 under the combination of UVA radiation + Sirtinol (SIRT1 inhibitor), or UVA radiation + miR-27a-5p mimic. SIRT1 activator resveratrol could reverse damage changes caused by UVA radiation. Besides, absent of SIRT1 or overexpression of miR-27a-5p increased cell apoptosis and induced cell arrest in G2/M phase. Taken together, these results demonstrated that UVA could influence a novel SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in skin primary fibroblasts, and may provide potential therapeutic targets for UVA-induced skin damage.

Focus issue introduction: advanced solid-state lasers.

This joint issue of Optics Express and Optical Materials Express features 28 state-of-the-art articles written by authors who participated in the international "Advanced Solid State Lasers" conference, held in Boston November 4-8, 2018. This review provides a summary of these articles that cover the spectrum of solid state lasers from materials research to sources and from design innovation to applications.

Feature issue introduction: Advanced Solid-State Lasers 2017.

The Advanced Solid State Lasers 2017 Conference (ASSL) was held from October 1 to 5, 2017. It was an extraordinary conference at the Nagoya Congress Center in Nagoya, Japan. ASSL 2017 again suggested an impressive platform where miscellaneous perceptions with a variety of approaches to optics, photonics, sensing, laser technology, laser systems, and solid state lasers were presented. This international meeting was highly selective, leading to high level contributions through one plenary conference, 17 invited presentations, 70 regular talks, and 121 posters. The present joint issue of Optics Express and Optical Materials Express features 27 articles written by ASSL 2017 authors and covering the spectrum of solid-state lasers from materials research to sources, and from design innovation to applications.

1.8 mJ, 3.5 kW single-frequency optical pulses at 1572 nm generated from an all-fiber MOPA system.

High-energy single-frequency optical pulses at 1572 nm were generated from an all-fiber MOPA system for atmospheric CO2 LIDAR system application. We report the experimental demonstration of 1.8 mJ, a peak power of 3.5 kW at the pulse repetition of 2.5 kHz, as well as 1.3 mJ, a peak power of 2.5 kW at the pulse repetition of 7.5 kHz single-frequency optical pulses at 1572 nm using single-mode large-core polarization-maintaining Er-Yb co-doped silicate glass fiber amplifiers pumped at 976 nm. To the best of our knowledge, this is the highest pulse energy of single frequency at 1572 nm from an all-fiber amplifier system.

Focus issue introduction: Advanced Solid-State Lasers (ASSL) 2016.

The editors introduce the focus issue on "Advanced Solid-State Lasers (ASSL) 2016", which is based on the topics presented at a conference of the same name held in Boston, USA, from October 30 to November 3, 2016. This focus issue, jointly prepared by Optics Express and Optical Materials Express, includes 20 contributed papers (14 for Optics Express and 6 for Optical Materials Express) selected from the voluntary submissions from attendees who presented at the conference and have extended their work into complete research articles. We hope this focus issue provides a useful link to the variety of topical discussions held at the conference and will contribute to the further expansion of the associated research areas.

Focus issue introduction: Advanced Solid-State Lasers (ASSL) 2015.

The editors introduce the focus issue on "Advanced Solid-State Lasers (ASSL) 2015", which is based on the topics presented at a congress of the same name held in Berlin, Germany, from October 4 to October 9, 2015. This focus issue, jointly prepared by Optics Express and Optical Materials Express, includes 23 contributed papers (17 for Optics Express and 6 for Optical Materials Express) selected from the voluntary submissions from attendees who presented at the congress and have extended their work into complete research articles. We hope this focus issue offers a good snapshot of a variety of topical discussions held at the congress and will contribute to the further expansion of the associated research areas.

The CDH1 -160C/A polymorphism is associated with breast cancer: evidence from a meta-analysis.

BACKGROUND: Numerous epidemiological studies have evaluated the association between the CDH1 -160C/A polymorphism and the risk of breast cancers. However, these studies have yielded conflicting results. To derive a more precise estimation of this association, this meta-analysis was conducted. METHODS: A comprehensive search using the keywords "CDH1," "E-Cadherin," "polymorphism," "SNP," and "variant" combined with "breast," "cancer," "tumor," or "carcinomas" was conducted. Pooled odds ratios (ORs) with 95 % confidence intervals (CIs) were appropriately calculated using a fixed effect or random effect model. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2009 checklist was used for this meta-analysis. RESULTS: Four publications including five studies were identified. It was found that the CDH1 -160C/A polymorphism was significantly associated with breast cancer risk in the dominant model (CA + AA vs. CC: OR = 1.207, 95 % CI = 1.031-1.412, P = 0.019). CONCLUSIONS: Our meta-analysis demonstrated that the -160C/A polymorphism in the CDH1 gene might contribute to breast cancer susceptibility. Further investigations using a much larger sample including different ethnicities are still needed to verify this association.

Cascaded interferometers structure based on dual-pass Mach-Zehnder interferometer and Sagnac interferometer for dual-parameter sensing.

In this article, we propose and demonstrate a cascaded interferometers structure based on a dual-pass Mach-Zehnder interferometer (DP-MZI) and a Sagnac interferometer (SI) for simultaneous measurement of strain and lateral stress. The cascaded interferometers configuration consists of a SI structure following with a MZI setup. By inserting a section of polarization-maintaining photonic crystal fiber (PM-PCF) in the sensing loop of the SI structure, an inline interference between the two orthogonal polarization modes of PM-PCF, as well as the interference between the sensing arm and the reference arm of the DP-MZI, i.e., the cascaded interferometers with dual interference, are realized. Theoretical study shows that the reflection spectrum of such cascaded interferometers is consisted of two parts: the big spectrum envelope owing to the SI and the fine interference fringes as a result of the DP-MZI. Experimental results show that the SI achieves the sensitivity of lateral stress and strain 1.28 nm/kPa, 0.78 pm/µÎµ, respectively, while the DP-MZI achieves -0.009 nm/kPa and 5.65 pm/µÎµ, demonstrating the ability for dual parameters measurement with high accuracy.

Focus issue introduction: Advanced solid-state lasers (ASSL) 2014.

The editors introduce the focus issue on "Advanced Solid-State Lasers (ASSL) 2014," which is based on the topics presented at a congress of the same name held in Shanghai, China, from October 27 to November 1, 2014. This focus issue, jointly prepared by Optics Express and Optical Materials Express, includes 28 contributed papers (21 for Optics Express and 7 for Optical Materials Express) selected from the voluntary submissions by attendees who presented at the congress and have extended their work into complete research articles. We hope this focus issue offers a useful snapshot of the variety of topical discussions held at the congress and will contribute to the further expansion of the associated research areas.

Monolithic polarization maintaining fiber chirped pulse amplification (CPA) system for high energy femtosecond pulse generation at 1.03 µm.

A monolithic polarization maintaining fiber chirped pulse amplification system with 25 cm Yb(3+)-doped high efficiency media fiber that generates 62 µJ sub-400 fs pulses with 25 W at 1.03 µm has recently been demonstrated.

Focus issue introduction: Advanced Solid-State Lasers (ASSL) 2013.

The editors introduce the focus issue on "Advanced Solid-State Lasers (ASSL) 2013," which is based on the topics presented at a congress of the same name held in Paris, France, from October 27 to November 1, 2013. This focus issue, jointly prepared by Optics Express and Optical Materials Express, includes 21 contributed papers (18 for Optics Express and 3 for Optical Materials Express) selected from the voluntary submissions from attendees who presented at the congress and have extended their work into complete research articles. We hope this focus issue offers a good snapshot of a variety of topical discussions held at the congress and will contribute to the further expansion of the associated research areas.

High-efficiency ytterbium-free erbium-doped all-glass double cladding silicate glass fiber for resonantly-pumped fiber lasers.

A highly efficient ytterbium-free erbium-doped silicate glass fiber has been developed for high-power fiber laser applications at an eye-safe wavelength near 1.55 µm. Our preliminary experiments show that high laser efficiency can be obtained from a relatively short length of the gain fiber when resonantly pumped at 1535 nm in both core- and cladding-pumping configurations. With a core-pumping configuration as high as 75%, optical-to-optical efficiency and 4 W output power were obtained at 1560 nm from a 1 m long gain fiber. When using a cladding-pumping configuration, approximately 13 W output power with 67.7% slope efficiency was demonstrated from a piece of 2 m long fiber. The lengths of silicate-based gain fiber are much shorter than their silica-based counterparts used in other experiments, which is significantly important for high-power narrow-band and/or pulsed laser applications.

Dihuangzicao Granules Regulate the AGE/RAGE/NF-κB Signaling Pathway to Inhibit Inflammation in Psoriatic Mice via Network Pharmacology and Experimental Validation.

AIM: Psoriasis is an immune-mediated skin disease that occurs worldwide and is characterized by high prevalence and chronicity. Psoriasis has a complex pathogenesis and is difficult to cure. Therefore, continuous exploration of the pathogenesis of psoriasis and the search for new drug treatment methods are crucial for improving treatment efficiency and reducing psychological damage to psoriasis patients. The active ingredients in Dihuang Zicao granules (DHZCG) can effectively treat psoriasis. Therefore, this study aimed to analyze the active ingredients of DHZCG and their potential mechanisms for treating psoriasis. METHODS: The effective components of DHZCG were screened via the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Genetic information for psoriasis was retrieved from the GeneCards, OMIM and DisGENET databases. Protein-Protein Interaction (PPI) analysis was performed, and component‒target‒disease networks were constructed. Important molecular biological processes and signaling pathways were screened via GO and KEGG analyses. Molecular docking of the active ingredients and key targets was performed via AutoDock Vina (1.1.2). A mouse model of psoriasis was established and divided into a control group, model group, low-dose DHZCG group (L-DHZCG), medium-dose DHZCG group (M-DHZCG), and high-dose DHZCG group (H-DHZCG). Hematoxylin and Eosin (HE) staining was performed to determine the pathological changes in the skin of each group of mice, and the Psoriasis Area Severity Index (PASI) score was used to assess skin damage. ELISA and RT‒ PCR were used to measure the levels of the inflammatory factors TNF-a, IL-17A, and IL-23 in the serum and skin tissue of the mice, respectively. Western blotting was used to analyze the expression of proteins related to the AGE/RAGE signaling pathway. Immunofluorescence was used to examine the expression of the inflammatory factor NF-kB. Immunohistochemistry was used to measure IL-1ß and TNF-a expression in skin tissues. RESULTS: Sixty genes associated with psoriasis treatment by DHZCG, including core genes encoding IL-6, TNF-a, AKT1, IL-1ß, TP53, NFKB1, BCL2, and MAPK3, were identified. Through the construction of a psoriasis mouse model, DHZCG treatment effectively reduced skin damage and significantly decreased the levels of the validated factors TNF-a, IL-17A, IL- 23, IL-1b, and NF-kB in the serum and damaged skin. Furthermore, the reduction in the levels of these inflammatory factors by DHZCG is associated with the downregulation of the AGE/RAGE signaling pathway. CONCLUSION: DHZCG reduces inflammation and alleviates psoriasis by downregulating the AGE/RAGE/NF-kB signaling pathway. This study is beneficial for providing a theoretical basis for the development of drugs for psoriasis and for offering personalized treatment strategies for the clinical management of psoriasis.

Ultrabroadband and >93% Microwave Absorption Enabled by "Doped" Water Meta-Atom Lattice with Subwavelength Thickness.

Perfect microwave absorbers, which absorb electromagnetic waves completely, play pivotal roles in electromagnetic shielding, and stealth technologies. Existing microwave absorber technologies rely on either electromagnetic properties of absorptive materials, the resonance behavior of meta-atoms, or a combination of both. So far, achieving simultaneous broadband absorption, high efficiency, and compact sizes remains a great challenge. Inspired by atomic doping techniques employed in conventional optical materials to broaden spectral bandwidths, a single-layer microfluidic metasurface microwave absorber is proposed with the assembly of two distinct types of water meta-atoms. By manipulating electromagnetic resonances of these water meta-atoms, the metasurface maintains impedance matching over a broad working range. A microwave absorber design with a thickness equivalent to 0.2 times the central wavelength is showcased, measuring over 93% absorption across both K and Ka bands (17.5-40.0 GHz). The results highlight unprecedented superiorities of microwave absorbers based on a 2D doped water meta-atom lattice when compared to previously reported metasurface absorbers utilizing identical meta-atoms. This absorber has advantages including small thickness, broad bandwidth, and cost-effectiveness, making it promising for applications in electromagnetic shielding, camouflage, and multi-spectral stealth.

Clinical significance and potential mechanism of AEBP1 in glioblastoma.

Glioblastomas (GBM), the most common primary brain tumor, lack accurate prognostic markers and have a poor prognosis. Our study was designed to identify effective biomarkers for GBM prognosis analysis and development of precise treatments. Differentially expressed genes (DEGs) between GBM patients and controls were analyzed from the Xena database and GEPIA. Based on the screened DEGs, univariate COX and LASSO regression analysis were performed to identify the most relevant genes associated with GBM prognosis. Genes highly expressed in GBM patients were selected to construct receiver operating characteristic analysis and enrichment analysis was constructed on groups of high and low expression of adipocyte enhancer-binding protein 1 (AEBP1). CIBERSORT, ssGSEA and ESTIMATE were used to perform immune infiltration analysis. About 3297 DEGs were identified using data from Xena database; 8 prognostic genes were identified. AEBP1, which plays a role in neuronal differentiation and development, was positively correlated in GBMs with immune infiltration; its high expression in cancer patients is associated with short overall survival and advanced tumor staging. This study suggests that AEBP1 could serve as a prognostic marker for GBMs and that patients with high expression may have a better response to immunotherapy.

Single-frequency gain-switched Ho-doped fiber laser.

We demonstrate a single-frequency gain-switched Ho-doped fiber laser based on heavily doped silicate glass fiber fabricated in-house. A Q-switched Tm-doped fiber laser at 1.95 µm was used to gain-switch the Ho-doped fiber laser via in-band pumping. Output power of the single-frequency gain-switched pulses has been amplified in a cladding-pumped Tm-Ho-codoped fiber amplifier with 1.2 m active fiber pumped at 803 nm. Two different nonlinear effects, i.e., modulation instability and stimulated Brillouin scattering, could be seen in the 10 µm-core fiber amplifier when the peak power exceeds 3 kW. The single-frequency gain-switched fiber laser was operated at 2.05 µm, a popular laser wavelength for Doppler lidar application. This is the first demonstration of this kind of fiber laser.

Optical fiber strain sensor using fiber resonator based on frequency comb Vernier spectroscopy.

A novel (to our best knowledge) optical fiber strain sensor using a fiber ring resonator based on frequency comb Vernier spectroscopy is proposed and demonstrated. A passively mode-locked optical fiber laser is employed to generate a phased-locked frequency comb. Strain applied to the optical fiber of the fiber ring resonator can be measured with the transmission spectrum. A good linearity is obtained between displacement and the inverse of wavelength spacing with an R(2) of 0.9989, and high sensitivities better than 40 pm/µÎµ within the range of 0 to 10 µÎµ are achieved. The sensitivity can be proportionally improved by increasing the length of the optical fiber ring resonator.