Elucidation of common molecular diagnostic biomarkers between chronic periodontitis and Parkinson's disease via bioinformatics analyses.

BACKGROUND AND OBJECTIVES: Parkinson's disease (PD) and chronic periodontitis (CP) are both inflammatory diseases; a correlation between the two diseases has been reported, but the underlying mechanisms of this association have not been investigated. We investigated the common molecular mechanisms between PD and CP and the role of immune cells in the pathogenesis of them using bioinformatics analyses to elucidate the association between the two diseases. METHODS: We obtained gene expression data from the Gene Expression Omnibus (GEO) database: GSE10334, GSE16134, and GSE23586 for CP gingival samples and GSE20146 for PD brain samples. Subsequently, we conducted an enrichment analysis of the differentially expressed genes (DEGs) using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses. Moreover, all DEGs were analysed for protein-transcription factor interactions and protein-immune cell co-expression. We constructed protein-transcription factor, protein-protein interaction (PPI), and protein-immune cell co-expression networks using the Cytoscape software. Moreover, we identified the hub genes and investigated them for potential diagnostic value. RESULTS AND CONCLUSION: We identified 99 DEGs in the three CP datasets, 520 DEGs in the PD dataset and found five common DEGs in the CP and PD datasets, namely CXCR4, CXCL8, CD19, RPTN, and SLC16A9. These common DEGs identified in our study may have a potential impact on disease pathogenesis through the involvement of CXCR4-CXCL8-CD19 protein-complexes in dendritic cells. Therefore, CD19, LCP2, CXCR4, and LYN could be used as target molecules for the clinical diagnosis of both diseases.

Biodegradable bilayer hydrogel membranes loaded with bazedoxifene attenuate blood-spinal cord barrier disruption via the NF-κB pathway after acute spinal cord injury.

After spinal cord injury (SCI), blood-spinal cord barrier (BSCB) disruption and hemorrhage lead to blood cell infiltration and progressive secondary injuries. Therefore, early restoration of the BSCB represents a key step in the treatment of SCI. Bazedoxifene (BZA), a third-generation estrogen receptor modulator, has recently been reported to inhibit inflammation and alleviate blood-brain barrier disruption caused by traumatic brain injury, attracting great interest in the field of central nervous system injury and repair. However, whether BZA can attenuate BSCB disruption and contribute to SCI repair remains unknown. Here, we developed a new type of biomaterial carrier and constructed a BZA-loaded HSPT (hyaluronic acid (HA), sodium alginate (SA), polyvinyl alcohol (PVA), tetramethylpropane (TPA) material construction) (HSPT@Be) system to effectively deliver BZA to the site of SCI. We found that HSPT@Be could significantly reduce inflammation in the spinal cord in SCI rats and attenuate BSCB disruption by providing covering scaffold, inhibiting oxidative stress, and upregulating tight junction proteins, which was mediated by regulation of the NF-κB/MMP signaling pathway. Importantly, functional assessment showed the evident improvement of behavioral functions in the HSPT@Be-treated SCI rats. These results indicated that HSPT@Be can attenuate BSCB disruption via the NF-κB pathway after SCI, shedding light on its potential therapeutic benefit for SCI. STATEMENT OF SIGNIFICANCE: After spinal cord injury, blood-spinal cord barrier disruption and hemorrhage lead to blood cell infiltration and progressive secondary injuries. Bazedoxifene has recently been reported to inhibit inflammation and alleviate blood-brain barrier disruption caused by traumatic brain injury. However, whether BZA can attenuate BSCB disruption and contribute to SCI repair remains unknown. In this study, we developed a new type of biomaterial carrier and constructed a bazedoxifene-loaded HSPT (HSPT@Be) system to efficiently treat SCI. HSPT@Be could provide protective coverage, inhibit oxidative stress, and upregulate tight junction proteins through NF-κB/MMP pathway both in vivo and in vitro, therefore attenuating BSCB disruption. Our study fills the application gap of biomaterials in BSCB restoration.

Human Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Attenuate Blood-Spinal Cord Barrier Disruption via the TIMP2/MMP Pathway After Acute Spinal Cord Injury.

After spinal cord injury (SCI), destruction of the blood-spinal cord barrier (BSCB) results in infiltration of blood cells, such as neutrophils and macrophages, leading to permanent neurological dysfunction. Previous studies have shown that human bone marrow mesenchymal stem cell (BMSC)-derived exosomes have a beneficial neuroprotective effect in SCI models. However, whether BMSC-Exos contribute to the integrity of the BSCB has not been clarified. The purpose of this study was to investigate the mechanism of BMSC-Exo-induced changes in the permeability of the BSCB after SCI. Here, we first used BMSC-Exos to treat an SCI rat model, showing that BMSC-Exos can inhibit BSCB permeability damage and improve spontaneous repair. Next, we found that tissue inhibitors of matrix metalloproteinase 2 (TIMP2) have been shown to play an important role in the function of BMSC-Exos by inhibiting the matrix metalloproteinase (MMP) pathway, thereby reducing the reduction of cell junction proteins. Therefore, we constructed siTIMP2 to knock out TIMP2 in BMSC-Exos, which caused the activity of BMSC-Exos to be significantly weakened. Finally, we constructed an in vitro model of BSCB with HBMECs and verified that TIMP2 in BMSC-Exos in vitro can also alleviate BSCB damage. This proof-of-principle study demonstrates that BMSC-Exos can preserve the integrity of the BSCB and improve functional recovery after SCI through the TIMP2/MMP signaling pathway.

Three⁃dimensional cephalometric measurement of craniofacial hard tissues with normal occlusion in Anhui area / 安徽医科大学学报

Objective@#To establish a three⁃dimensional cephalometric standardized value database for Anhui province , to study the craniofacial characteristics of normal adult population in Anhui , China and to provide effective information for making orthodontic and orthognathic surgery treatment plans of the craniofacial deformity.@*Methods@#160 normal occlusal volunteers were selected according to the inclusive and exclusive criteria and were photographed by Cone Beam CT (CBCT) . The coordinate system was established for the 3D model reconstructed with Dolphin based on CBCT data , and 38 hard tissue landmarks were located. 40 lines and 20 angles were defined and measured , and measurements were analyzed with SPSS 17. 0 , to evaluate craniofacial features of the overall , midface , maxilla , mandible , alveolar bone , and dentition of the standard population.@*Results@#The 3D cephalometric measurements of craniofacial hard tissues of normal adults in Anhui were obtained. The symmetry of craniofacial hard tissues was good both in males and females with the maximum asymmetry ratio of 2. 2% and the minimum of 0. 01% . The gender comparisons showed that most of lines measured of males were larger than those of females (P< 0. 01) , while there was no significant difference in the measurements of the midline deviation of upper and lower mandible , the relative deviation of the maxillary and mandible and the protrusion of upper and lower incisor. Additionally , there was no statistical significance in angles measured between genders , except for the occlusal plane angle. The results also showed that Chinese had unique facial features based on the measured data , compared with other ethnic populations.@*Conclusion@#The normal occlusal and maxillofacial hard tissue of Anhui Han has good symmetry. The gender obviously influences line measurements , with generally the measurements of males larger than females , but it has no effect on most angle measurements.

Combating COVID-19 with integrated traditional Chinese and Western medicine in China

COVID-19, an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread throughout the world. China has achieved rapid containment of this highly infectious disease following the principles of early detection, early quarantine and early treatment with integrated traditional Chinese and Western medicine. The inclusion of traditional Chinese medicine (TCM) in the Chinese protocol is based on its successful historic experience in fighting against pestilence. Current findings have shown that the Chinese medicine can reduce the incidence of severe or critical events, improve clinical recovery and help alleviate symptoms such as cough or fever. To date there are over 133 ongoing registered clinical studies on TCM/integrated traditional Chinese and Western medicine. The three Chinese patent medicines (/ (Forsythiae and Honeysuckle Flower Pestilence-Clearing Granules/Capsules), (Honeysuckle Flower Cold-Relieving Granules) and (Stasis-Resolving & Toxin-Removing) were officially approved by the National Medical Products Administration to list COVID-19 as an additional indication. The pharmacological studies have suggested that Chinese medicine is effective for COVID-19 probably through its host-directed regulation and certain antiviral effects.

Four-component instructional design model and its application on clinical teaching in orthodontics / 中华医学教育探索杂志

As an important sub-discipline of dentistry, orthodontics is regarded as the post-graduate education for postgraduates and advanced doctors both at home and abroad. Orthodontics is a professional, practical and systematic discipline, therefore, the conventional education model is hard to get a good result. In this study, a new idea of four-component instructional design model (4C/ID) guided by "integration" was explored in the education of orthodontics, combined with the author's study experience in the University of Pennsylvania. The 4C/ID mainly included four elements: learning tasks, supportive information, procedural information and part-task practice, which emphasized in providing learners a set of real, specific and work-oriented complete learning tasks, so as to replace "partial task learning" to "overall task learning". This study explored the 4C/ID and its application on clinical teaching in orthodontics.

Four-component instructional design model and its application on clinical teaching in orthodontics / 中华医学教育探索杂志

As an important sub-discipline of dentistry,orthodontics is regarded as the post-graduate education for postgraduates and advanced doctors both at home and abroad.Orthodontics is a professional,practical and systematic discipline,therefore,the conventional education model is hard to get a good result.In this study,a new idea of four-component instructional design model(4C/ID)guided by "integration" was explored in the education of orthodontics,combined with the author's study experience in the University of Pennsylvania.The 4C/ID mainly included four elements: learning tasks,supportive information,procedural information and part-task practice,which emphasized in providing learners a set of real,specific and work-oriented complete learning tasks,so as to replace "partial task learning" to "overall task learning".This study explored the 4C/ID and its application on clinical teaching in orthodontics.

One stage posterior pedicle screw fixation combined with transpedicle intervertebrae titanic mesh autograft for type C thoracolumbar fractures / 中华创伤杂志

Objective To evaluate the therapeutic effeet of one stage posterior pedicle screw fixation combined with transpedicle intervertebrae titanic mesh autograft versus traditional posterior and anterior approach surgery in the treatment of severe thoracolumbar fractures.Methods A retrospective case-control study was done on 40 cases of type C thoracolumbar fractures admitted from January 2012 to December 2015.There were 28 males and 12 females,with age range of 20-48 years (mean,30.6 years).The fractures were located at T11 in 4 cases,at T12 in 16,at L1 in 14,and at L2 in 6.All cases were divided into two groups (with 20 cases per group) according to treatment method difference.The cases in posterior group had one stage posterior pedicle screw fixation combined with transpedicle intervertebrae titanic mesh autograft,and the others in posterior and anterior group were treated by traditional posterior and anterior approach surgery.The operation duration,volume of blood loss,hospital stay,kyphosis correction rate,visual analogue scale (VAS) and Oswestry disability index (ODI) were compared between the two groups.Results All patients were followed up for 10-46 months (mean,28.6 months).In posterior group,the average operation time was 154 minutes,the average blood loss was 564 ml,the postoperative suction drainage was 180.5 ml,the time period between surgery and recovery of walking ability was 5.9 days,and the hospital stay was 10.6 days.While in posterior and anterior group,the average operation time was 248.5 minutes,with the average blood loss of 960.8 ml,the postoperative suction drainage of 359.2 ml,the time duration between surgery and recovery of walking ability of 8.4 days,and the hospital stay of 14.5 days (P ＜ 0.05).At one week and six months postoperatively,the height ratio of anterior edge,Cobb angle,VAS and ODI in both groups were improved compared to the preoperative status (P ＜ 0.05).But there was no significant difference of these indicators between two groups (P ＞ 0.05) except for lumbar back pain one week after operation (P ＞ 0.05).Conclusions Through single pathway of posterior approach,one stage posterior pedicle screw fixation combined with transpedicle intervertebrae titanic mesh autograft can complete reduction of fractured vertebral body,spinal cord decompression,and anterior-middle column stability reconstruction.Compared with traditional combined posterior and anterior approach surgery,this technique has many advantages including simpler manipulation,less invasion,shorter rehabilitation time and equal bony fusion and hence is an ideal option for type C thoracolumbar fractures.

Unilateral atlantoaxial pedicle screw fixation plus structural iliac bone graft for treatment of unstable atlas fractures / 中华创伤杂志

Objective To evaluate the efficacy of unilateral atlanto-axial transpedicle screw fixation plus iliac bone graft for treatment of unstable atlas fractures combined with unilateral pedicle dysplasia or comminuted fractures.Methods A retrospective case control study was made on 44 patients with unstable atlas fractures surgically treated between January 2012 to June 2016.Unilateral atlanto-axial transpedicle screw fixation combined with iliac bone graft was performed for 22 patients combined with unilateral pedicle dysplasia or comminuted fractures in Group A[15 males,seven females;(37.5 ± 13.4)years],and bilateral atlanto-axial transpedicle screw fixation combined with iliac bone graft was performed for 22 patients without unilateral pedicle dysplasia or comminuted fractures in Group B [14 males,eight females;(38.1 ± 13.3)years].Between-group differences were compared concerning operation time,intraoperative blood loss,length of hospital stay,success rate of screw placement,postoperative atlantoaxial stability,surgery-related complications,visual analog scale (VAS),Japanese orthopedic association score (JOA) and bone fusion.Results Mean duration of follow-up was 28.4 months (range,14-48 months).In Group A,operation time was (123.4 ± 18.2) min,blood loss was (218.5 ± 80.2) ml,hospital stay was (7.1 ± 1.0)d,success rate of screw placement was 100％,postoperative atlanto-axial stability of all patients was obtained,and no complications occurred.In Group B,operation time was (173.4 ± 12.4) min,blood loss was (318.2 ± 61.7) ml,hospital stay was (7.2 ± 0.8) d,success rate of screw placement was 100％,postoperative atlanto-axial stability of all patients was obtained,and no complications occurred.There were significant differences in operation time and blood loss between the two groups (P ＜0.01),while not in hospital stay,success rate,postoperative atlant-oaxial stability,complication incidence,VAS and JOA (P ＞ 0.05).Conclusion Both treatments are effective,but unilateral atlanto-axial transpedicle screw fixation combined with iliac bone graft is associated with relatively shorter operation time and less blood loss and hence is considered as a better choice for treatment of unstable atlas fractures.

High quality nursing in gastroscopy / 中国内镜杂志

Objective To analyze the effect of high quality nursing in gastroscopy examination. Method 1 300 pa﹣tients underwent gastroscopy from March 2014 to June 2014 were randomly divided to control group (n= 650) and intervention group (n= 650). Patients in control group received routine nursing, and patients in intervention group received high quality nursing intervention besides the routine nursing. Patients anxiety level, open state of esophageal meatus, one-time intubation success rate, and time-consumed in gastroscopy of the two groups were compared. Result Anxiety score between the two groups at the time of reservation was not statistically significant (P> 0.05), anxiety scores of the intervention group (31.40 ± 4.20) was lower than that of control group before gas﹣troscopy, and the difference was statistically significant (P<0.01). Open rate of esophageal meatus, one-time intuba﹣tion success rate, and time-consumed in gastroscopy of the intervention group (97.1%, 95.1%and 4.52 ± 1.23 min) were significantly better than those of the control group (79.4%, 82.3%and 5.42 ± 1.53 min). Conclusion The high quality nursing in gastroscopy can alleviate the anxiety level of patients, improve the success rate of gastroscopy, re﹣duce the pain of patients, and it is worthy of clinical promoting.

Experimental animal models of intervertebral fusion-induced adjacent segment degeneration / 中国组织工程研究

BACKGROUND:Upright rat intervertebral disc degeneration model can prove that intervertebral fusion can aggravate the adjacent-segment intervertebral disc degeneration, but cannot affirm that spinal non-fusion technology has more advantages. OBJECTIVE:Base on the affirmed intervertebral disc degeneration models of Beagle dog, we performed intervertebral fusion to affirm whether or not the intervertebral fusion can aggravate the adjacent-segment intervertebral disc degeneration. METHODS:Twelve Beagle dogs were randomly divided into two groups. The control group animals were stabbed percutaneous to injure L5/6 disc. The experimental group animals were stabbed percutaneous to injur L5/6 disc. One month later, we made L4/5 intervertebral fusion. At 3 and 6 months postoperatively, lumbar MRI was conducted. L5/6 discs were harvested and subjected to histological observation and PCR. RESULTS AND CONCLUSION:(1) MRI:in the experimental group, 3 and 6 months after fusion, different degrees of intervertebral disk herniation appeared. In the control group, no obvious intervertebral disk herniation appeared. (2) Histological observation:6 months after fusion, in the experimental group, intervertebral disc annulus and nucleus pulposus were disordered;there was a gap in the fold, and the number of nucleus pulposus cel s decreased. In the control group, nucleus pulposus and anulus fibrosus boundary was clear;col agen was loose and arranged regularly. Cel number was more. Anulus fibrosus was close to normal. Col agen fibers were regular and dense. In the experimental group, 3 and 6 months after fusion, type II col agen-positive cel s were less than the control group (P<0.05). Moreover, type II col agen-positive cel s were less at 6 months than at 3 months in the experimental group (P<0.05). (3) PCR:Bone morphogenetic protein 15 and tissue inhibitor of matrix metal oproteinase 1 gene expression was higher in the experimental group than in the control group at 3 and 6 months after fusion (P<0.05). Bone morphogenetic protein 15 and tissue inhibitor of matrix metal oproteinase 1 gene expression was higher in the experimental group at 6 months than that at 3 months (P<0.05). (4) These results suggest that intervertebral fusion can aggravate the adjacent-segment intervertebral disc degeneration.

The curative effect of acute pancreatitis by octreotide and anisodamine / 中国现代医生

Objective To explore the clinical curative effect of acute pancreatitis by octreotide and anisodamine. Methods Eighty cases acute pancreatitis in our hospital were analyzed,were divided into two groups,the control group 30 cases and observation group 50 cases. Results The general information on sex、age、 duration of disease distribution of acute pancreatitis two groups had no difference (P>0.05),the abdominal pain relief time、 serum amylase、 urine amylase、hospitalization time of acute pancreatitis of observation group was better than control group , the CPR、TNF-α、IL-4、IL-8、IL-10 and clinical treatment efficiency of acute pancreatitis of observation group was better than control group (P<0.05),the differences were statistically significanct. Conclusion The clinical symptom of acute pancreatitis by octreotide combined with anisodamine is obviously improved ,clinical therapeutic effect is increased.

Bone graft fusion combined with internal fixation restores vertebral height after thoracolumbar fracture and dislocation / 中国组织工程研究

BACKGROUND:Spinal canal decompression and fusion combined with pedicle screw fixation has become the main method for the treatment of thoracolumbar fracture and dislocation. OBJECTIVE:To restore the vertebral height of the patients with thoracolumbar fracture and dislocation who underwent subtotal resection and decompression through titanium cage packed with autologous cancel ous bone and pedicle screw fixation. METHODS:Thirty-one patients with thoracolumbar fracture and dislocation treated in the Shanghai 6th People’ s Hospital of Shanghai Jiao Tong University from February 2007 to September 2011 were selected. Al the patients received subtotal resection, spinal canal decompression and titanium cage packed with autologous cancel ous bone and pedicle screw fixation. Imaging examination was performed before and after treatment to observe the recovery of vertebral sequence, vertebral height and Cobb angle, as wel as the recovery of nerve function. RESUTLS AND CONCLUSION:The patients were fol owed-up for 12 months, and al the patients healed wel after treated with titanium cage packed with autologous cancel ous bone and pedicle screw fixation, and there was no loosening, shedding or breakage after pedicle screw fixation. During reexamination, the grafts fusion, good deformity correction, basic recovery of vertebral height and good recovery of Cobb angle were observed. Seven cases had neurological function recovery, and among them, six cases raised for one degree, one case raised for two degrees, and another 22 cases without recovery. Case analysis and relative researches showed that subtotal resection and decompression and titanium cage packed with autologous cancel ous bone and pedicle screw fixation can reduce the loss of correction of the patients with thoracolumbar fracture and dislocation, restore the vertebral height, and enhance the stability of the vertebral body.

Topping-off technique for the protection of degenerative intervertebral disc / 中国组织工程研究

BACKGROUND:Topping-off technique can be used for fixation treatment through the combination of fusion and interspinous dynamic device, in order to prevent or slow down the adjacent lumbar segment degeneration. OBJECTIVE:To obverse the protective effect of Topping-off technique (posterior lumbar interbody fusion procedure combined with the fixation of dynamic interspinous device Coflex) for the degenerative intervertebral disc. METHODS:A total of 32 patients with degenerative lumbar diseases who had been treated with Topping-off technique were included in this study. The Oswestry disability index, the Japanese Orthopaedic Association scores, range of motion for Coflex implanted segment and during the relative signal intensity of the Coflex implanted segment in MRI image were recorded and calculated preoperatively and the entire fol ow-up period. RESULTS AND CONCLUSION:Al patients were fol owed-up for 20.6 months averagely. Up to the last fol ow-up, the Oswestry disability index and Japanese Orthopaedic Association scores were significantly improved when compared with those before treatment (P<0.001). There was no significant difference in the range of motion for Coflex implanted segment before and after treatment (P=0.19). The relative signal intensity of the Coflex implanted segment was significantly improved when compared with that before treatment (P<0.01). The clinical application of the Topping-off technique showed a protective effect on the intervertebral disc.

Study on the allocation of medical care resources in Shanghai / 中华医院管理杂志

Based on the current status of allocation, demands and utilization of medical care resources and the needs for future development in Shanghai, the overall objectives, principles, key plans of allocation of medical care resources in the 12th Five-years Plan in Shanghai and the leading role of health bureaus at all levels were discussed.

The overall progress and challenges of Shanghai health care for the last 30 years since reform and opening up / 中华医院管理杂志

Marked by the Third Plenary Session of the Eleventh Central Committee of the CPC, Shanghai health care started a journey of reform and opening up as with other sectors. This essay reviews various stages Shanghai health care experienced and the achievements for the last 30 years since reform and opening up, and analyzes the challenges Shanghai health care faces currently. On this basis, the essay summaries the experiences and lessons learned from Shanghai health care reform and development.

Basic framework and thought of deepening reform of Shanghai healthcare system / 中华医院管理杂志

Under the general requirements of China's national health reform, the basic framework of Shanghai's health reform was constructed at five different aspects the long term vision, goal, strategies, measures, supportive reform activities. The general thought of Shanghai's health reform can be concluded into two aspects: first, establishing a basic healthcare system of covering both urban and rural residents universally; second, enhancing the foundation of enabling sustainable health development.

In vivo and in vitro animal models of intervertebral disc degeneration and repair / 中国组织工程研究

BACKGROUND:Animal models can be used to study specific scientific problems of intervertebral disc biology.Model of disc degeneration is mainly used to resolve the relevant disease mechanisms and scientific and security issues of the treatment.OBJECTIVE:To summarize currently used experimental animal models of intervertebral disc degeneration study,and to dynamically observe and confirm the pathological process of disc degeneration based on disc imaging,morphology,biomechanics and bi(o)chemicel changes.METHODS:Using "intervertebral disc degeneration,animal models,in vivo,in vitro" in English as the search words,Cochrane Library (No.1 2009),Cochrane Library Database of Controlled Clinical Trials (No.1 2009),MEDLINE from 1990 to March 2009,EMbase from 1990 to March 2009,Current Controlled Trials,and the National Research Register were retrieved.Literature was limited to English language.The disc imaging,morphology,biomechanical end biochemical composition and other indicators,as well as the pathological process of disc degeneration served as the evaluation indices.The articles related to the intervertebral disc cell culture models,the whole disc tissue culture model,mechanical model,injury model,biological model,genetically modified models,spontaneous models were included.The repetitive researches and those unrelated to animal models of intervertebral disc degeneration were excluded.RESULTS AND CONCLUSION:The establishment of a reliable animal model can provide favorable conditions for studying the pathogenesis of intervertebral disc degeneration,at the same time,provides a good experimental vehicle for various researches about the repair treatment of intervertebral disc degeneration.Animal models of intervertebral disc degeneration can be divided into two categories:in vitro models and in vivo models of disc degeneration and repair.The former can be assigned into disc cell culture models and whole disc tissue culture model;the latter is assigned into mechanical models,injury models,biological models,genetically modified models,spontaneous models and so on.The above models are commonly used in the study of the occurring mechanism of disc degeneration,as well as the feasibility and effectiveness of a variety of treatments.However,there is still no generally accepted animal models as an ideal disc degeneration model,various types of models reported have their own advantages and disadvantages.