Building an improved transcription factor-centered yeast one hybrid system to identify DNA motifs bound by protein comprehensively.

BACKGROUND: Identification of the motifs bound by a transcription factor (TF) is important to reveal the function of TF. Previously, we built a transcription factor centered yeast one hybrid (TF-Centered Y1H) that could identify the motifs bound by a target TF. However, that method was difficult to comprehensively identify all the motifs bound by a TF. RESULTS: Here, we build an improved TF-Centered Y1H to comprehensively determine the motifs bound by a target TF. Recombination-mediated cloning in yeast was performed to construct a saturated prey library that contains 7 random base insertions. After TF-Centered Y1H screening, all the positive clones were pooled together to isolate pHIS2 vector. The insertion regions of pHIS2 were PCR amplified and the PCR product was subjected to high-throughput sequencing. The insertion sequences were then retrieved and analyzed using MEME program to identify the potential motifs bound by the TF. Using this technology, we studied the motifs bound by an ethylene-responsive factor (BpERF2) from birch. In total, 22 conserved motifs were identified, and most of them are novel cis-acting elements. Both the yeast one hybrid and electrophoretic mobility shift assay verified that the obtained motifs could be bound by BpERF2. In addition, chromatin immunoprecipitation (ChIP) study further suggested that the identified motifs can be bound by BpERF2 in cells of birch. These results together suggested that this technology is reliable and has biological significance. CONCLUSION: This method will have wide application in DNA-protein interaction studies.

The role of predictive and preview effects in Mongolian reading: evidence from eye movements.

Introduction: The research on contextual predictability in reading has been thoroughly investigated in the context of horizontal text comprehension. However, the performance of contextual predictive effects in Mongolian vertical reading remains unknown. Methods: To explore this, we conducted an eye-tracking study using a boundary paradigm. Our study aimed to investigate contextual predictability and preview effects in Mongolian reading. Results: We found significant main effects of predictability and previewing on temporal indicators. However, there were no significant effects on skipping rates, and no interaction between predictability and previewing was observed. Discussion: We speculate that the unique reading orientation and writing features of Mongolian, compared to horizontally read phonetic scripts, reduce the parafoveal processing of preview information, leading to lower skipping rates in Mongolian reading.

The role of orthographic and phonological processing during reading Chinese sentences: Evidence from eye movements.

The role of phonological and orthographic processing and their time course during lexical processing and sentence reading remain controversial. By adopting a misspelled-characters disruption paradigm and eye-tracking technique, we manipulated the writing for the first characters of two-character target words to investigate the relative role of orthographic and phonological processing on word recognition in Chinese reading. There are four conditions: (a) correct character, (b) misspelled character with a stroke missing, (c) misspelled homographic character, and (d) misspelled homophonic character. The results showed that homophonic errors caused more disruptions than other conditions in the early (first-pass reading times) and later (total reading time) stages of lexical processing during Chinese reading. Homographic errors and omitted stroke errors lead to equal disruptions at the early stage of word recognition, but homographic errors cause more disruptions at the later stage. These results suggest that orthography plays a dominant role in word recognition during Chinese reading, whereas phonology plays a weaker and more limited role. The direct access and dual-rote hypothesis may well explain the mechanism of lexical processing in Chinese reading.

Estimation of the number of workers with occupational noise-induced hearing loss from manufacturing industry in China, 2020 / 中国职业医学

{L-End}Objective To estimate the number of workers with occupational noise-induced hearing loss (ONHL) in the manufacturing industry of China in 2020. {L-End}Methods Multiple data sources were integrated to collect information on workers in the manufacturing industry from the Occupational Diseases and Hazards Monitoring Information System under China Disease Prevention and Control Information System. The total number of workers was split based on age from the 2018 Fourth National Economic Census and the 2020 National Survey of Occupational Disease Hazards. Additionally, data on the prevalence of hearing loss in the general population was adjusted based on the age composition of Jilin Province, and noise prevalence was standardized based on the age composition of the employed population in the Seventh National Population Censu. Attribution fractions (AF) was estimated. The prevalence ratio (PR) was calculated by the prevalence of hearing loss in the occupational noise-exposed workers and general population. The proportion and attributable cases (AC) of ONHL cases in all hearing loss cases were estimated for occupational noise-exposed workers. The number of ONHL was estimated based on AF and the total number of workers with hearing loss. {L-End}Results In 2020, a total of 30 059 525 workers were exposed to occupational noise in the manufacturing industry in China, with noise-exposed prevalence and standardized noise-exposed prevalence of 28.94% and 28.52%, respectively. The prevalence of hearing loss among occupational noise-exposed workers increased with age, and a total of 8 054 789 workers suffered from hearing loss. Most of the cases were at the age between 45-<55 years old. The total PR and 95% uncertainty interval (UI) was 2.83 (2.58-3.06) and the total AF and 95%UI was 64.63% (61.22%-67.30%), and both peaks were in the age of 30-<45 years. The AC and 95%UI was 5 205 980 (4 930 620-5 420 345) persons, and most of the cases were at the age between 40-<55 years. {L-End}Conclusion Occupational noise poses a serious threat to the hearing health of workers in the manufacturing industry of China, especially among middle-aged and young adults.

Effect of light therapy on psychological stress and sleep quality in elderly patients with coronary heart disease combine with dsychological barriers / 中国实用护理杂志

Objective:To investigate application effect of light therapy in elderly patients with coronary heart disease combine with dsychological barriers.Methods:Totally, 112 coronary heart desease patients combine with dsychological barriers treated in Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University from October 2018 to December 2019 were selected, and they were divided into experimental group and control group according to random number table method, each group including 56 patients. The control group received routine care. On the basis of this, the experimental group received light therapy for 4 weeks. Before and after intervention, the effects were assessed by Symptom Checklist 90 (SCL-90) and Pittsburgh Sleep Quality Index (PSQI), respectively.Results:After intervention, the scores of somatization, compulsion, depression, anxiety, fear and total scores of SCL-90 in the experimental group were (1.26 ± 0.13), (1.43 ± 0.08), (1.17 ± 0.13), (1.14 ± 0.10), (1.34 ± 0.07), (120.55 ± 11.87) points, significantly lower than (1.83 ± 0.53), (1.66 ± 0.59), (1.71 ± 0.12), (1.76 ± 0.13), (1.41 ± 0.12), (130.69 ± 14.16) points in the control group, the differences were statistically significant ( t values were 2.88-27.79, all P<0.05); and the scores of subjective sleep quality, sleep duration, daytime dysfunction and total scores of PSQI in the experimental group were (0.96 ± 0.19), (1.74 ± 0.36), (1.12 ± 0.22), (10.22 ± 0.60) points, significantly lower than (1.12 ± 0.20), (1.89 ± 0.25), (1.33 ± 0.32), (10.81 ± 0.73) points in the control group, the differences were statistically significant ( t values were 2.44-4.54, all P<0.05). Conclusions:Light therapy can effectively alleviate the psychological stress response in elderly patients with coronary heart disease combine with dsychological barriers, and improve sleep quality.

Imaging features of struma ovarii in conventional ultrasound and CEUS / 中国医学影像技术

Objective To investigate sonographic characteristics of struma ovarii with conventional ultrasound and CEUS.Methods Ultrasound images of 65 patients with struma ovarii confirmed by pathology were retrospectively reviewed and analyzed,5 patients were examined with CEUS simultaneously.Results In all 65 patients,lesions in 14 (14/65,21.54 ％) were multocular,49 (49/65,75.34％) were cystic-solid component,2 (2/65,3.08％) were solid.Lesions in 39 (39/65,60.00％) contained dense latticed separation.The abundant blood flow was found in 18 cases (18/65,27.69％) with Doppler examination.Five cases were examined with CEUS,including multilocular lesions of 2 cases and cystic-solid lesions of 3 cases.Regular middle-degree intensity of cyst wall and septa were seen in all 5 cases.Among cystic-solid lesions of 3 cases,lesions of non-enhance pattern was seen in the solid areas of 1 case,irregular middle-high degree intensity pattern were seen in the solid areas of 2 cases,while non-enhance pattern could be seen in part of the solid areas of these 2 cases.All the cystic areas of these 5 cases showed non-enhance pattern.Conclusion The sonographic appearances of struma ovarii are usually multilocular or multilocular with solid component.Because of strum ovarii's special characteristic pathologic components,the imaging features of strum ovarii in conventional ultrasound and CEUS are atypical,thus preoperative diagnosis is quite difficult.

Imaging features of struma ovarii in conventional ultrasound and CEUS / 中国医学影像技术

Objective To investigate sonographic characteristics of struma ovarii with conventional ultrasound and CEUS.Methods Ultrasound images of 65 patients with struma ovarii confirmed by pathology were retrospectively reviewed and analyzed,5 patients were examined with CEUS simultaneously.Results In all 65 patients,lesions in 14 (14/65,21.54 ％) were multocular,49 (49/65,75.34％) were cystic-solid component,2 (2/65,3.08％) were solid.Lesions in 39 (39/65,60.00％) contained dense latticed separation.The abundant blood flow was found in 18 cases (18/65,27.69％) with Doppler examination.Five cases were examined with CEUS,including multilocular lesions of 2 cases and cystic-solid lesions of 3 cases.Regular middle-degree intensity of cyst wall and septa were seen in all 5 cases.Among cystic-solid lesions of 3 cases,lesions of non-enhance pattern was seen in the solid areas of 1 case,irregular middle-high degree intensity pattern were seen in the solid areas of 2 cases,while non-enhance pattern could be seen in part of the solid areas of these 2 cases.All the cystic areas of these 5 cases showed non-enhance pattern.Conclusion The sonographic appearances of struma ovarii are usually multilocular or multilocular with solid component.Because of strum ovarii's special characteristic pathologic components,the imaging features of strum ovarii in conventional ultrasound and CEUS are atypical,thus preoperative diagnosis is quite difficult.

Effects of different follow-up methods on quality of life of patients with Heroin Spongiform Lekoencepha-lopathy / 实用医学杂志

Objective To explore the effects of different follow-up methods on the quality of life of pa-tients with Heroin Spongiform Lekoencephalopathy(HSLE)in 6 months after discharge. Methods A total of 59 HSLE patients were assigned to control group(n = 29)and experimental group(n = 30). Control group received regular telephone interview,and experimental group the follow-up from an interdisciplinary cooperation team in 6 months after discharge. Social support rate and the quality of life in 2 groups were compared using the Social Sup-port Rating Scale(SSRS)and the Quality of life for Drug Addicts(QOL-DA)for 6 months after discharge. Re-sults After the intervention,the scores of SSRS and the QOL-DA of experimental group indicated a marked im-provement compared with those of control group. The score of QOL-DA of two groups was all improved and intra-group comparison had statistical differences(P<0.05). Conclusions Follow-up can improve social support rate and the quality of life of HSLE patients,and the effect of follow-up from a multidisciplinary team is better than that of regular telephone interview.

Comparison of three herbal proparations in airway remodeling in rats with chronic asthmatic / 中成药

AIM To research the antiasthmatic effects of Zuogui Pills,Yougui Pills and Yupingfeng Powder and their mechanism of action.METHODS Male Brown Norway rats were randomly divided into five groups.OVA was used to induce the model of chronic asthma.Five groups of asthmatic rats were given Zuogui Pills,Yougui Pills,Yupingfeng Powder by gavage once daily for 14 d,respectively.The normal and the model group were given normal saline.Penh value,serum Thl,Th2 inflammatory cytokines,and CROT and ACTH protein levels were determined.Pathological changes of airway remodeling were measured.RT-PCR method was used to measure the expression of CRH mRNA in hypothalamus,and TGF-β1 and Smad3 mRNA in lung tissues.RESULTS Penh values of Yupingfeng Powder group and Yougui Pills group were significantly lower than that of Zuogui Pills group.The Thl inflammatory cytokine of Yupingfeng Powder group was significantly higher than that of Zuogui Pills group,while the Th2 cytokine was significantly lower than that of Zuogui Pills group.In Yougui Pills group,only IL-5 was significantly lower than that of Zuogui Pills group.Serum CROT,ACTH protein and CRH mRNA of Yupingfeng Powder group were significantly higher than those in Zuogui Pills group.No significant difference was found between Yupingfeng Powder group and Zuogui Pills group with regard to TGF-β1 and Smad3 mRNA.Smad3 mRNA of Yougui Pills group was significantly higher than that of the model group.Airway remodeling was significantly reduced by Yupingfeng Powder and Zuogui Pills.The number of goblet cells in Yougui Pills group was significantly lower than that in Zuogui Pills group.CONCLUSION All the three formulaes possess significant antiasthma effects.Regulation effects of Yupingfeng Powder on Thl/Th2 balance,HPA and TGF-β1/Smad3 pathway are strong.Compared with Zuogui Pills,Yougui Pills possesses weaker TGF-β1/Smad3 regulative effects and stronger HPA regulative effects.

Analysis of prognosis factors for acute stroke patients / 中华神经医学杂志

Objective To analyze the risk factors associated with prognosis of stroke patients.Methods A total of 551 stroke patients from 5 third class A hospital in Guangzhou from May 2014 to August 2014,were recruited.The clinical data and prognosis 3 months after follow up of these patients were collected and Logistic regression analysis was used to analyze the influencing factors of stroke prognosis.Results Good prognosis was noted in 311 patients (56.4％) and bad one was noted in 240 patients (43.6％);age,education degree,watcher,temperature,NIHSS and Barthel index (BI) scores,hospital stays,stroke frequency,dysphagia,urinary incontinence,coronary heart disease,smoking history and total cholesterol levels between patients with good or bad prognosis were significantly different (P＜ 0.05).Logistic regression analysis showed that major factors for stroke prognosis included age,temperature,NIHSS and BI scores,hospital stays,stroke frequency and urinary incontinence.Conclusions Patients with age≥60 years,temperature＞37 ℃,high NIHSS scores,serious defects of activities of daily living,long hospital stays,recurrent stroke,post-stroke urinary incontinence trend to have poor prognosis,therefore,these patients should be targeted to plan intervention program to improve the life quality.

Correlation analysis between post-stroke constipation and brain injury / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the incidence of constipation after stroke and explore the relationship between post-stroke constipation and brain injury.</p><p><b>METHODS</b>Using a self-designed questionnaire, we collected the general information of 723 inpatients from 10 hospitals in Guangzhou, including the general demographic information, related factors of brain injury, defecation and previous history.</p><p><b>RESULTS</b>The total incidence of post-stroke constipation was 34.6% in these patients. The incidence of constipation was 31.0% in ischemic stroke patients, 44.8% in hemorrhagic stroke patients, and 47.4% in patients with cerebral hemorrhage and infarction, showing a significant difference between stroke types (χ(2)=12.369, P=0.002). In terms of stages following stroke, the incidence was 41.6% in the acute phase, 31.5% in the recovery phase, and 22.6% in the sequelae phase. In light of lesion locations, the incidence was significantly higher in patients with basal ganglia involvement than in those without (P<0.001). Logistic multivariate analyses showed that stroke type, post-stroke stage, lesion number and basal ganglia involvement were significantly associated with the incidence of constipation.</p><p><b>CONCLUSION</b>Constipation often occurs in the acute stage following stroke (especially hemorrhagic stroke), and the incidence is higher in patients with basal ganglia involvement.</p>

Nursing and study on correlation among cognitive dysfunction and post-stroke constipation / 中国实用护理杂志

Objective To understand the status of cognitive dysfunction among new onset constipation after stroke and to explore the relationship between them.Methods With a self-designed questionnaire,the general information of 723 inpatients from 10 hospitals in Guangzhou were collected,including general demographic information,stroke situation,defecation situation and Mini-mental State Examination (MMSE)etc.Results The incidence of new-onset constipation,cognitive dysfunction(PSCD)after stroke was respectively 34.6％ and 62.4％.Among whom 31.0％ in ischemic stroke,44.8％ in hemorrhagic stroke,47.4％ in cerebral hemorrhage and infarction.There was statistical significance between stroke type and constipation,and ischemic stroke had statistical significance compared to other two types.During the acute phase reached 41.6％,the recovery phase 31.5％ and the sequelae phase 22.6％.The rates of constipation patients with cognitive dysfunction were higher than patients without cognitive dysfunction,and the difference in directional force,memory,attention and calculation force,reading and expression was statistically significant respectively,among which expression was the most relevant.The difference in the incidence of constipation among different cognitive dysfunction groups was statistically significant,and they were positively correlated,compared with normal cognitive function,the light,medium and severe cognitive dysfunction was 1.519,2.879,3.064 times respectively.Conclusions Medical staff need to be alerted to the possibility that patients with impared cognitive function may be at greater risk of constipation.The preventive and treatment measures should be emphasized in order to improve patients' cognitive function.

Prognostic predictive value of quantitative electroencephalography for patients with large middle cerebral artery infarction / 国际脑血管病杂志

Objective To study the prognostic predictive value of quantitative dectroencephalography (qEEG)for patients with large middle cerebral artery infarction (LMCAI).Methods The scores of routine electroencephalography (EEG),qEEG and the Glasgow Coma Scale (GCS) of the patients within 72 hours after symptom onset were recorded.The short-term prognosis (death or survival) was evaluated at 1 month after the onset.The long-term prognosis (good or poor) was evaluated at 3 months after the onset.All the observed data in each prognostic group were compared.Results A total of 105 patients were included in the study.There were significant differences in the margin of amplitude integrated electroencephalogram (aEEG) (upper margin:19.11 ± 7.80 μV vs.11.87 ±6.41 μV;t =2.392,P =0.019; lower margin:11.90 ± 4.78 μV vs.7.58 ± 4.15 μV; t =3.327,P =0.022),Synek-classification (x2 =48.114,P =0.000) between the short-term survival group and the death group; in patients with left LMCAI,there were significant differences in the absolute energy of the β-activity (13.16 ±12.66 μV2 vs.19.20 ±17.96 μV2;t =-2.781,P =0.039),spectral edge frequency 95％ (SEF95％) (9.17 ± 3.24 Hz vs.10.36 ± 3.76 Hz; t =-5.614,P =0.002) between the short-term survival group and the death group.There were significant differences in the age (59.33 ±13.67 years vs.68.87± 10.473 years; t =-3.215,P =0.002),GCS scores (10.86±2.80 vs.9.21 ±2.51;t =2.511,P =0.015),SEF95％ (13.80 ±5.40 Hz vs.10.93 ±4.68 Hz; t ＝2.311,P =0.024) and sides of infarction (x2 =4.737,P =0.030) between the long-term good prognosis group and the poor prognosis group.Conclusion qEEG can be used as an effective means of monitoring for evaluating the prognosis of patients with LMCAI.

Research on relevant factors affecting results of extubation of trachea cannula in neurocritical care patients / 中华急诊医学杂志

Objective To Explored the relative factors which caused the extubation failure in neurological intensive care unit (NICU).Methods It was a retrospective study.40 cases of patients who met the criteria,were brought into statistical analysis.They were admitted in NICU in Nan Fang Hospital from December 2008 to February 2011.The name,sex,age,diagnosis,respiratory parameters,24 hours discrepancy quantity,sputum,and Glasgow Coma Scale,Full Outline of UnResponsiveness Scale were recorded.SPSS 13.0 was used as statistic software.P ＜ 0.05 was considered statistically significant.Results Both in extubation successful and failure groups,GCS and Four were significantly different (all P ＜ 0.05).Howerer,there were no statistically significant in the other factors.There were significantly differences between GCS and Four in predicting extubation results (P =0.012).Logistic multiple regression showed that Four and GCS grade were predictive factor of extubation failure (P =0.041).Conclusions The result suggests that it is statistically significant to use GCS and Four as factors to predict extubation results.It can be widely used to help medical personnels monitoring the changes of patients'clinical conditions,judging prognosis,and making treatment plan in NICU.Wether other factors would effect the extubation results,more prospective,randomized controlled studies were needed.

Prediction of malignant course in large middle cerebral artery infarction by electroencephalography / 国际脑血管病杂志

Objective To explore the predictive value of early electroenphalography (EEG) for a malignant course in patients with large middle cerebral artery infarction (LMCAI).Methods Thirty-seven patients (20 patients with a malignant and 17 with a benign course) with stroke of >50% of the middle cerebral artery territory in early CT/MRI scan were included;Glasgow-Pittsberg Coma Scale (24 ±1 vs. 30 ±4, P =0. 003) and National Institutes of Health Stroke Scale (23 ±3 vs. 16 ±4, P =0.000) in the group with a malign course were higher than those in the group with a benign course. Early EEG was recorded within 24 h after ischemic stroke. The correlation between the change characteristics of EEG and a malignant course in patients with LMCAI was analyzed. Results The contralateral occipital background frequencies < 8 Hz (17/20 vs. 3/20, P =0.000), β frequency within the focus ≤20 Hz (19/26 vs. 7/26, P= 0-001), EEG non-reaction to stimuli (11/12 vs. 1/12, P= 0.002),slowing affecting the whole hemisphere in the lesion (17/24 vs. 7/24, P = 0. 008) and focal slowing contralateral to the lesion (16/19 vs. 3/19, P =0. 000) were significantly related with a malignant course. Whereas the contralateral occipital background frequencies ≥8 Hz (14/17 vs. 3/17, P =0. 000),β frequency >20 Hz within the focus (10/11 vs. 1/11, P =0. 001) were related with a benign course. Conclusions Early EEG has a certain predictive value for a malignant course in patients with LMCAI, and it may be used as one of the bedside monitoring approaches of LMCAI.

Relationship between invasion and changes of mRNA epression of MMP-2, TIMP-2 and CD147 in human pituitary adenoma / 中华内分泌外科杂志

Objective To investigate the relationship between tumor invasion and changes of mRNA expression of MMP-2, TIMP-2 and CD147 in human pituitary adenoma. Methods 60 patients with pituitary adenoma were devided into two groups, invasive and non-invasive group, by MRI. The expression level of MMP-2,TIMP-2 and CD147 in the samples of pituitary adenoma was measured by semiquantitative reverse transcriptasepolymerase chain reaction (RT-PCR). Pearson analysis was used to reveal the correlation between the expression of each marker. Results The mRNA expression level of MMP-2 and CD147 was significantly higher in invasive pituitary adenoma group than that in non-invasive group (P ＜ 0. 01 ) while the mRNA expression level of TIMP-2was lower in invasive pituitary adenoma group than that in non-invasive group (P ＜0.01 ). According to Pearson analysis, the mRNA expression of MMP-2 was positively correlated with CD147 in invasive pituitary adenoma (r=0. 69, P ＜ 0. 05 ), and M MP-2 was negatively correlated with TIMP-2 in non-invasive pituitary adenoma (r =-0.68, P ＜ 0.05 ). Conclusions The invasion of human pituitary adenoma are closely related to the low expression level of TIMP-2 as well as the high expression level of MMP-2 and CD147. MMP-2, TIMP-2 and CD147 can be used as indicators of tumor invasion of human pituitary adenoma.

Stereotactic Minimally Invasive Aspiration for Small Thalamic Hemorrhage A Clinical Study / 国际脑血管病杂志

Objective:To explore the feasibilitv of stereotactic minimally invasive aspiration of small thalamic bemorrhage.Methods:Twenty-two patients with small thalamic hemowhage(5 to 10 mL)were divided into two groups:a stereotactic group(n=10)and a control group(n= 12).The patients in the stereotactic group received stereomctic minimally invasive puncture and drainage of hematomas.According to the condition,repeated infusion of urokinase(10-20 kU) into the hematoma cavities were administered 12 hours after the procedure,and the hematomas were irrigated and drained so as to removal of them completely after retaining for 2-4 hours, The appropriate symptomatic treatment was administered in the patients in both groups.National Institutes of Health Stroke Scale(NIHSS)scores were determined 14 and 30 days before and after the treatment in all the patients.The reductiom of the NIJSS scores (as compared with those before treatment)were calculated at day 14 and 30 respectively after the treatment. Results:The reductiom of the NIHSS scores in the stereotactic group at day 14 and 30 were significantly higher than those in the control group.It was suggested that the neurological functional recovery of the patients was faster after stereotmtic minimally invasive puncture and drainage of intracranial hematorna in the stereotactic group.Concision:The stereotactic minimally invasive puncture and drainage of intracranial hematoma may significantly improve the outcome in patients with small thalamic hemorrhage.

Cyclosporine Inhibits Apoptosis in Experimental Murine Xerophthalamia Conjunctival Epithelium / 华中科技大学学报（医学）（英德文版）

This study examined the inhibitory effect of topical cyclosporine (CsA) treatment on conjunctiva epithelial apoptosis in a murine model of xerophthalamia. Dry eye was induced in 3 groups of C57BL6 mice by subcutaneous injection of scopolamine (t.i.d) and exposure to an air draft and low-humidity environment for 16 h each day for 12 days. The dry eye control group received no topical treatment; another group received 1 μL of 0.05 % CsA topically (t.i.d, dry eye+CsA); and the third group received 1 μL of the castor oil vehicle of CsA topically (t.i.d, dry eye + vehicle). Normal mice were used as untreated controls. Twelve days later, the mice were killed, and their conjunctivas were excised. The number of the conjunctival goblet cells was counted in tissue sections stained with periodic acid Schiff (PAS) reagent. Their conjunctiva epithelium had been investigated by immuno-histochemical staining to detect the goblet cells and the expression of Caspase-3, Bax and bcl-2.Our results showed that compared with dry eye control and dry eye mice + vehicle groups, the number of conjunctival epithelial goblet cells was significantly greater in the untreated controls and dry eye mice receiving CsA (P ＜0.01 for both groups). There was no significant difference in the number of conjunctival epithelial goblet cells between the dry eye control and dry eye+vehicle group. It was also true of the number of conjunctival epithelial goblet cells when comparison was made between the normal group and the dry eye+CsA group. Expressions of Caspas-3 and Bax were increased and ex-pression of bcl-2 was decreased in conjunctival epithelial cells in dry eye control and dry eye mice+vehicle groups. There was a significant positive correlation between goblet cell number and the number of cells that expressed bcl-2, and a negative correlation between goblet cells and Caspase-3 and Bax expression. It is concluded that the topical use of CsA could significantly reduce conjuncti-val epithelial apoptosis and protect goblet cell against the loss in experimental murine xerophathala-mia. Inhibition of apoptosis appears to be a key mechanism responsible for the therapeutic effect of CsA on xerophthalamia.

Cyclosporine inhibits apoptosis in experimental murine xerophthalamia conjunctival epithelium / 华中科技大学学报（医学）（英德文版）

This study examined the inhibitory effect of topical cyclosporine (CsA) treatment on conjunctiva epithelial apoptosis in a murine model of xerophthalamia. Dry eye was induced in 3 groups of C57BL6 mice by subcutaneous injection of scopolamine (t.i.d) and exposure to an air draft and low-humidity environment for 16 h each day for 12 days. The dry eye control group received no topical treatment; another group received 1 microL of 0.05% CsA topically (t.i.d, dry eye+CsA); and the third group received 1 microL of the castor oil vehicle of CsA topically (t.i.d, dry eye + vehicle). Normal mice were used as untreated controls. Twelve days later, the mice were killed, and their conjunctivas were excised. The number of the conjunctival goblet cells was counted in tissue sections stained with periodic acid Schiff (PAS) reagent. Their conjunctiva epithelium had been investigated by immuno-histochemical staining to detect the goblet cells and the expression of Caspase-3, Bax and bcl-2. Our results showed that compared with dry eye control and dry eye mice + vehicle groups, the number of conjunctival epithelial goblet cells was significantly greater in the untreated controls and dry eye mice receiving CsA (P < 0.01 for both groups). There was no significant difference in the number of conjunctival epithelial goblet cells between the dry eye control and dry eye+vehicle group. It was also true of the number of conjunctival epithelial goblet cells when comparison was made between the normal group and the dry eye+CsA group. Expressions of Caspas-3 and Bax were increased and ex-pression of bcl-2 was decreased in conjunctival epithelial cells in dry eye control and dry eye mice+vehicle groups. There was a significant positive correlation between goblet cell number and the number of cells that expressed bcl-2, and a negative correlation between goblet cells and Caspase-3 and Bax expression. It is concluded that the topical use of CsA could significantly reduce conjunctival epithelial apoptosis and protect goblet cell against the loss in experimental murine xerophathalamia. Inhibition of apoptosis appears to be a key mechanism responsible for the therapeutic effect of CsA on xerophthalamia.