RESUMEN
We have examined the number of genes coding for a group of high-molecular-mass rhoptry protein(s) in the malaria parasite Plasmodium yoelii, and studied variation in the gene family within the parasite's genome. A region of the genes was amplified using oligonucleotides based on conserved DNA sequences and the products cloned. The sequences could be divided into 7 groups by restriction-fragment-length polymorphism. Further variation was detected by sequence analysis; 11 different sequences were detected in the 16 clones analyzed. The genes in the family were distributed on 6 chromosomes probably at 9 or more loci.
Asunto(s)
Genes Protozoarios , Plasmodium yoelii/genética , Proteínas Protozoarias/genética , Animales , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , Variación Genética , Ratones , Datos de Secuencia Molecular , Plasmodium yoelii/inmunología , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Proteínas Protozoarias/inmunología , Alineación de Secuencia , Homología de Secuencia de Ácido NucleicoRESUMEN
The gene B protein (GBP) is one of the products of the LmcDNA16 gene family, a cluster of related but non-identical genes that are differentially-expressed during the Leishmania life cycle. This protein, which is found on the surface of infective stage parasites, contains an extensive region of proline-rich amino acid repeats, constituting 45% of the total protein. The structure and stability of these repeats have been investigated in a number of L. major strains by polymerase chain reaction (PCR) amplification and Southern blotting. Data reported in this paper demonstrate variability between strains with respect to the number of repeats encoded by GBP, although those strains isolated within adjacent geographical regions have conserved repeat structures. The data also reveal that some parasite lines have additional repeat sequences within a second, related gene in the LmcDNA16 array. Western blotting experiments have established that these sequences are expressed in vivo, indicating that L. major strains are heterogeneous in their surface complement of gene B repeat-containing proteins.
Asunto(s)
Variación Genética/genética , Leishmania major/genética , Proteínas de la Membrana/genética , Secuencias Repetitivas de Ácidos Nucleicos/genética , Secuencia de Aminoácidos , Animales , Genes Protozoarios , Genoma de Protozoos , Leishmania major/química , Proteínas de la Membrana/química , Datos de Secuencia Molecular , Familia de Multigenes , Prolina/genética , Proteínas Protozoarias/biosíntesis , Proteínas Protozoarias/genética , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
We describe the deduced amino acid sequence for a gene encoding a high molecular mass rhoptry protein of Plasmodium yoelii. The sequence was obtained from an EcoRI genomic clone that overlaps a short DraI fragment isolated previously. The open reading frame consists of 2294 codons and putative hydrophobic signal and membrane anchor sequences were identified. Similarity with sequence from a clone coding for part of a Plasmodium vivax reticulocyte-binding protein was noted. Based on the sequence and location of the protein and the biological properties of antibodies that react with it, we propose that this may be an erythrocyte-binding protein.
Asunto(s)
Genes Protozoarios , Plasmodium yoelii/genética , Proteínas Protozoarias/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Sondas de ADN/genética , ADN Protozoario/genética , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Peso Molecular , Familia de Multigenes , Sistemas de Lectura Abierta , Plasmodium vivax/genética , Estructura Secundaria de Proteína , Proteínas Protozoarias/química , Homología de Secuencia de AminoácidoRESUMEN
The RAD51 gene is a homologue of Escherichia coli recA which plays a central role in homologous recombination and DNA repair. This paper describes the identification of the RAD51 gene from the trypanosomatid parasite Leishmania major. The LmRAD51 gene codes for a 377 amino acid polypeptide with a predicted molecular mass of 41259 Da that is highly homologous to the Rad51 family of proteins. Recombinant L. major Rad51 protein (LmRad51) was over-expressed in a bacterial expression system, purified to homogeneity and shown to bind DNA and exhibit DNA-stimulated ATPase activity, consistent with previously reported biochemical characteristics of Rad51 protein. Although LmRad51 expression is below the level of detection in exponentially growing cultures of Leishmania, high levels of LmRad51 mRNA and protein expression can be detected following exposure to the DNA-damaging agent phleomycin. LmRAD51 is one of the first examples of a DNA damage-inducible gene to be characterised in Leishmania, and will be invaluable in studying the contribution of homologous recombination to Leishmania virulence.
Asunto(s)
Adenosina Trifosfatasas/genética , Adenosina Trifosfatasas/metabolismo , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Genes Protozoarios , Leishmania major/genética , Adenosina Trifosfatasas/química , Secuencia de Aminoácidos , Animales , Clonación Molecular , ADN Protozoario/metabolismo , Proteínas de Unión al ADN/química , Proteínas de Unión al ADN/aislamiento & purificación , Humanos , Leishmania major/efectos de los fármacos , Leishmania major/crecimiento & desarrollo , Leishmania major/metabolismo , Datos de Secuencia Molecular , Fleomicinas/farmacología , Recombinasa Rad51 , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Análisis de Secuencia de ADNRESUMEN
The genomic organisation of the genes coding for a group of high molecular mass rhoptry proteins of the rodent malaria parasite Plasmodium yoelii YM was investigated using blotting, two dimensional gel electrophoresis and restriction fragment length analysis. The genes were found on chromosomes 1, 5, 6 and 10, with the possibility that related genes were also present on chromosomes 3 and 4. On chromosome 1 the genes were located close to one end, whereas they were present at both ends of chromosome 5, 6 and 10. Two genes, e3 and e8, that had been partially characterised previously were present on chromosomes 5 and 1, respectively. Based on an analysis of the 3' end of the genes, three subfamilies present on chromosomes 1, 5 and 6, and 10, respectively, were identified.
Asunto(s)
Genoma de Protozoos , Plasmodium yoelii/genética , Proteínas Protozoarias/genética , Animales , Southern Blotting , Mapeo Cromosómico , Electroforesis en Gel de Agar , Genes Protozoarios/genética , Plasmodium yoelii/químicaAsunto(s)
Familia de Multigenes , Plasmodium yoelii/genética , Proteínas Protozoarias/genética , Secuencia de Aminoácidos , Animales , Mapeo Cromosómico , Expresión Génica , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Proteínas Protozoarias/inmunología , Proteínas Recombinantes/inmunología , Recombinación Genética , Homología de SecuenciaRESUMEN
A 235 kD rhoptry protein produced by the malaria parasite, Plasmodium yoelii is the target of antibodies that protect mice against blood-stage challenge with the virulent YM strain. In the protected animals the parasites are confined to reticulocytes and the course of parasitaemia is reminiscent of an avirulent 17X strain infection. The DNA coding for the rhoptry protein has been identified as a multigene family containing at least four members. Sequence analysis of short DNA clones has identified the C-terminus of the protein; a preliminary analysis of longer clones confirms that the genes are polymorphic. The possible implications of these findings for the biology of the parasite are discussed.
Asunto(s)
Antígenos de Protozoos/genética , Plasmodium yoelii/genética , Secuencia de Aminoácidos , Animales , Antígenos de Protozoos/inmunología , Secuencia de Bases , Datos de Secuencia Molecular , Familia de MultigenesRESUMEN
The LmcDNA16 locus of Leishmania major contains three highly related genes HASPA1, HASPA2 and HASPB, encoding hydrophilic, acylated surface proteins and a tandem pair of unrelated sequences, SHERP1 and SHERP2, coding for a small, hydrophilic protein that localizes to the endoplasmic reticulum and outer mitochondrial membrane. Differential regulation of these genes results in expression of a subset of the HASP proteins and SHERP only in infective stage parasites. To assess the contribution of these molecules to parasite virulence, the diploid LmcDNA16 gene locus has been removed by targeted gene deletion. Homozygous null mutants have precise deletions of both alleles and exhibit no HASP or SHERP expression. They are at least as virulent as wild-type parasites in macrophage invasion and intracellular survival assays, both in vitro and in vivo. Conversely, null mutants engineered to overexpress the entire LmcDNA16 gene locus are unable to survive within the intramacrophage environment despite their differentiation into infective metacyclic parasites. Both null and overexpressing null parasites show increased sensitivity to complement-mediated lysis, suggesting perturbation of their surface architecture. Avirulence in overexpressing parasites correlates with selective depletion of a specific lipid species, decreased expression of the major surface glycoprotein GP63, but no significant downregulation of the glycoconjugate lipophosphoglycan.