RESUMEN
Microsomal preparations from rats treated with 2-acetylaminofluorene (2-AAF) or with 4-dimethylaminoazobenzene (4-DAB), alone or followed by phenobarbital (PB), showed almost complete loss of microsomal proteins of molecular weights higher than 60,000, as shown by Sephadex G-150 gel filtration technique. Induction of a number of microsomal proteins in the lower ranges of molecular weights was also recorded due to the treatment of these two hepatocarcinogens with and without the use of PB as a promoter. These modifications in the protein patterns of microsomes might be due to altered genetic expression resulting in uncontrolled cell division/cell cycle.
Asunto(s)
Carcinógenos/toxicidad , Hígado/efectos de los fármacos , Hígado/metabolismo , Microsomas Hepáticos/efectos de los fármacos , Microsomas Hepáticos/metabolismo , Proteínas/efectos de los fármacos , Proteínas/metabolismo , 2-Acetilaminofluoreno/administración & dosificación , 2-Acetilaminofluoreno/toxicidad , Administración Oral , Animales , Carcinógenos/administración & dosificación , Femenino , Inyecciones Intraperitoneales , Peso Molecular , Biosíntesis de Proteínas , Ratas , Ratas Wistar , p-Dimetilaminoazobenceno/administración & dosificación , p-Dimetilaminoazobenceno/toxicidadRESUMEN
Tap roots of Potentilla fulgens L. traditionally chewed along with betel nut (Areca catechu) and betel leaves (Piper betel), are commonly used by local practitioners for various types of ailments. The crude methanolic extract of the roots was tested for its effects in normoglycemic and alloxan-induced diabetic mice. Hypoglycemic activity was observed to be dose- and time- dependent. The extracts reduced blood glucose level 2 h following administration in both normal and alloxan-induced diabetic mice. In alloxan-induced diabetic mice blood glucose was markedly reduced by 63%, while in normal mice a 31% reduction was observed 24 h after the effective dose of extract was administered. Further, in the diabetic mice a prolonged anti-hyperglycemic action was observed where glucose levels was, found to be significantly low (79%) when compared with control even on the third day. Glucose tolerance was also improved in both normal and diabetic mice. The results were compared against those of insulin, glibenclamide, metformin, and the probable mechanism of action is discussed.