[Clinicopathological and molecular features of small round cell sarcoma of bone and soft tissue: a study of 72 cases].

Objective: To investigate the clinicopathological, immunohistochemical and molecular features of small round cell sarcoma (SRCS) of the bone and soft tissue, and to compare the diagnostic value of different techniques. Methods: Seventy-two cases of SRCS of the bone and soft tissue diagnosed at People's Hospital, Peking University from January 2016 to March 2020 were recruited and retrospectively analyzed for pathological morphology, immunophenotype and fluorescence in situ hybridization (FISH) data. Next generation sequencing (NGS) was performed on 13 difficult cases. Results: In the study cohort, the patients ranged in age from 4-55 years, with a male predominance. The most Ewing's sarcomas and osteosarcomas occurred in the bone, while CIC-rearranged sarcomas, BCOR-rearranged sarcoma, synovial sarcoma, extraskeletal myxoid chondrosarcoma and FUS-NFATc2 rearranged sarcoma occurred in soft tissue. Histologically, all cases were composed predominantly of small round cells. Most cases were positive for vimentin and CD99, and showed a variable reactivity for neurogenic markers. Muscle marker and epithelial marker were negative for most cases. Combined with clinical features, histopathologic findings, immunophenotype, FISH and NGS, we diagnosed 46 Ewing sarcomas, 14 osteosarcomas, 3 CIC-rearranged sarcomas, 1 BCOR-rearranged sarcoma, 1 synovial sarcoma, 1 clear cell soft tissue sarcoma, 1 extraskeletal myxoid chondrosarcoma, 1 FUS-NFATc2 rearranged sarcoma, and 4 undifferentiated small round cell sarcomas. Conclusions: SRCS of bone and soft tissue is a group of malignant mesenchymal tumors based on morphological features. Most cases can be diagnosed with a combination of clinical characteristics, morphological features and immunohistochemical phenotype, while some cases require such further tests as FISH and NGS technologies, and NGS can be useful in diagnosing and categorizing SRCS.

[Clinicopathological features of myeloid sarcoma and DLBCL in the breast: a comparative study].

Objective: To study the clinicopathological features, diagnosis and differential diagnosis of myeloid sarcoma of the breast. Methods: Ten cases of myeloid sarcoma (MS) and 19 cases of diffuse large B cell lymphoma (DLBCL) of the breast were selected from Peking University People's Hospital from February 2005 to September 2019. The cases were evaluated by microscopy and immunohistochemistry basing on WHO classification (2008 and 2017). Results: For the 10 cases of MS, the mean and median age was 33.8 and 31 years (range 23 to 47 years) respectively. All patients presented with breast masses; six presented with B symptoms (6/10); and LDH level was elevated in four patients. The largest tumor dimension was 1.0 to 5.3 cm (mean 2.7 cm). All 10 patients had history of acute myeloid leukemia (AML), and in one patient, the AML occurred after chemotherapy for hydatidiform mole. One case was classified as M0, four were M2, two were M4 and three were M5. For the AML, all patients received chemotherapy and nine were treated by allogeneic hematopoietic stem cell transplant (allo-HSCT) and the breast masses occurred4 months to 2 years post-transplant. Using Ann Arbor staging, five cases were stage Ⅰ, three were stage Ⅱ, and 2 were stage Ⅳ. The MS was found in the left breast (two cases); right breast (three cases) and both breasts (five cases). Lymphocyte in peripheral blood, B symptom and site of lesion had statistical significance between myeloid sarcoma and DLBCL(P<0.05). The tumor cells were primitive, expressing MPO, CD43, CD117, etc. All ten patients had follow-up information, and the median survival period was 14.4 months (range 1 to 50 months). Seven patients died. The prognosis of patients with MS was worse than DLBCL(P=0.002). Conclusions: The clinical history, pathologic morphology, immunophenotyping and molecular studies are very important for diagnosing MS tumors in the breast, and MS may occur after allo-HSCT for AML. Tumor resection, chemotherapy, radiotherapy and donor lymphocyte infusion are recommended for treatment. The prognosis is poor.

[Analysis of dental caries and related factors in preschool children in Qingdao in 2018].

Dental caries are common diseases in children and often affect their growth and development. In order to understand the dental caries status of preschool children in Qingdao, a total of 1 690 children aged 3-6 years were screened from 13 kindergartens in two districts of Qingdao. The oral health examination was conducted according to the WHO standard, including the decayed-missing-filled index and pigmentation status. The prevalence of caries, the mean decayed, missing and filled tooth (dmft) and the mean dmfs were calculated. The results showed that the prevalence of deciduous teeth caries of children was 65.3% (n=1 103) with the mean dmft and dmfs about 3.2 and 5.4. There was a statistically significant difference in the prevalence of deciduous teeth caries among different age groups (P<0.001). There was a statistically significant difference between the prevalence of deciduous teeth caries with and without pigmentation (P<0.001). The prevalence of caries in preschool children in Qingdao is high and more attention should be paid to their dental caries.

Sectional anatomy of the olfactory pathways.

AIM: The purpose of this study was to provide practical anatomic data for imaging diagnosis of olfactory pathways and operation of nasal cavity and anterior cranial fossae. METHODS: Sectional anatomy of olfactory pathways were investigated specially on 17 sets of Chinese adult cadavers and 9 sets of serial magnetic resonance (MR) imaging of normal adult on serial transverse, sagittal and coronal sections respectively. RESULTS: We recognized olfactory bulb, olfactory tract, medial and lateral olfactory striae, olfactory trigone, anterior perforated substance and piriform lobe on transverse, sagittal and coronal sections respectively. On the 5-7 serial coronal sections from crista galli of ethmoid bone to the optic chiasm, the cusp ellipse olfactory bulb and the triangular tract were situated in the shallow part of the olfactory sulcus. CONCLUSION: The olfactory bulb and olfactory tract lay tightly on the ethmoidal cribriform plate and jugum sphenoidale, in the olfactory cistern of the shallow part of the olfactory sulcus, the ethmoid sinus and sphenoid sinus inferiorly.

[Study on the correlation between depression and emotion regulation strategies in the elderly residents].

Objective: To explore the cross-sectional study of depressive and emotional regulation strategies of elderly residents in Yinchuan and their relationship. Methods: A total number of 1 043 elderly cases, including 505 from 5 nursing institutions and 538 from 5 communities in Yinchuan city were chosen, from March to May, 2016. Depression survey using the Geriatric Depression Scale (GDS), emotional adjustment strategies were conducted using the Gross Emotional Regulatory Questionnaire and the Rumination Scale. Results: The overall incidence rate of depression among the elderly residents appeared as 32.0%, with 35.5% in the communities and 28.3% in the nursing homes and the difference was statistically significant (χ(2)=6.187, P<0.05). There was no significant difference in the incidence of depression among the elderly and the aged in the community (χ(2)=0.066, P=0.798) by the trend analysis method. The score of GDS for the 1 043 elderly was (9.1±5.7) points. The scores on cognitive reappraisal in emotional adjustment strategies and on expression inhibition were (26.7±8.0) and (16.3±6.6) points respectively. The score of the rumination was (34.5±11.7) points. There were significant differences in cognitive reappraisal scores on different sex, hobbies and physical exercise (all Ps<0.05) among the elderly. There were significant differences in rumination scores among the elderly with different age, marital status, cultural level, interest (all Ps<0.05) and in the scores of expression inhibition among the elderly with different levels of education and income (all Ps<0.05). There appeared a negative correlation between the depression scores and cognitive reappraisal (r=-0.400, P<0.01) but a positive correlation between the scores of depression and rumination (r=0.652, P<0.01). Conclusion: The incidence of depression identified in the elderly residents appeared high and was associated with emotional adjustment strategies, in Yinchuan city.

[Effect of jia wei yubingfengsan on bacterial adhesion to mouse tracheain chronic bronchitis model].

The experiment was designed to test the effect of Jia Wei Yubingfengsan (JW-YBFS) in chronic bronchitis of mice model. Two groups of mice model was used, one of which received JW-YBFS by oral administration another received only water as control experiment. The accurate quantification of bacterial adhesiveness by culture method showed that in JW-YBFS receiving mice viable counts of bacteria is much less than the control mice. In addition pathological examination showing the injury of tracheate mucosal epithelium was not found in JW-YBFS receiving mice while control mice showing typical chronic bronchitis lesion. The results demonstrated that JW-YBFS plays significantly role in bronchitis mice model. Our experimental data are in good agreement with Chinese medical conclusion, which means Fu Zheng Gu Ben.