Detection of spoilage-causing yeasts and bacteria in tchapalo, the Ivorian traditional sorghum beer.

In this study, we aimed to analyse the spoilage potential of the isolated yeast, LAB and AAB species. Thus, 11 strains were inoculated at 0·3% (v/v) into a sterile filtered tchapalo and stored for 3 days at ambient temperature (27-30°C). All the tested strains grew well or remained stable except for Limosilactobacillus fermentum and Pediococcus acidilactici, which decreased throughout the storage time. A significant decrease of total soluble solids was observed only for Saccharomyces cerevisiae (from 7·8 to 5·8 °Brix) and Meyerozyma guilliermondii (from 7·8 to 5·5 °Brix). The tchapalo samples inoculated with the LAB strains Weissella paramesenteroides, P. acidilactici, L. fermentum and the yeast strain Candida tropicalis were judged similar to the control by the panellists. However, the strains of Lacticaseibacillus paracasei and Latilactobacillus curvatus (LAB), S. cerevisiae, M. guilliermondii and Kluyveromyces marxianus (yeasts) and Acetobacter pasteurianus and A. cerevisiae (AAB) induced the spoilage of the tchapalo appearance, smell and/or taste. In the spoiled tchapalo quantitative and qualitative modification of some volatile compounds (VOCs), such as lilac aldehyde, ethyl acetate, ethyl hexanoate, ethyl octanoate and phenethyl acetate, were observed. These results provide information about the microorganisms that need to be removed to extend the shelf life of tchapalo.

[Miliary tuberculosis in children. Report of 89 cases in the pediatric department, Abidjan ]. / La milliaire tuberculeuse chez l'enfant a propos de 89 cas colliges au centre anti tuberculeux d'adjame.

UNLABELLED: The HIV pandemic has led a resurgence of tuberculosis which did reach developing as well as developed countries. In Côte d'Ivoire, tuberculosis represented 40-50 per cent of HIV opportunist infections. The incidence rate of this infection, in 2004 was 110/100,000 inhabitants all type of tuberculosis together. As we know, a few studies haven't been devoted to child military pulmonary tuberculosis. Our objectives in this study are to precise circumstances of diagnosis, to assess treatment of the miliary tuberculosis to children below 15 years, to analyze evolving aspects of military tuberculosis. Retrospective study of medical hill of children center anti tuberculosis from 1st December 1999 to 31st December 2002. In the result, miliary tuberculosis is the third extra pulmonary tuberculosis, and concerns children from 6 to 11 years leaving in the social precarious the age where miliary tuberculosis is the most frequent. HIV seroprevalence rate is 40.62 per cent Clinical signs are: Cough expectoration and impregnation signs among which first have fever, weight loss. The radiological signs are opacity in the shape of seeds of millet. The treatment is efficacy if it is correctly administered. CONCLUSION: Diagnostic of miliary pulmonary tuberculosis is essentially radiologic. The rate of cure of 94% could be improved if health authorities, population and the government combine their efforts to struggle against tuberculosis.