[Isolation and multiplication of rabies virus in cell lines under conditions of routine rabies diagnosis]. / Izolacja i namnazanie wirusa wscieklizny na liniach komórkowych w warunkach rutynowej diagnostyki wscieklizny.

Isolation of rabies street virus from brains of animals with rabies was performed on cells of mouse neuroblastoma, infecting cells with suspension of tested brain tissue diluted 1:10, incubating at 37 degrees C in standard incubator (culture in Legroux bottles and Leighton tubes with rubber stoppers) and in 37 degrees C in an incubator with moist chamber and regulated flow of carbon dioxide (Mirco-Well plates, bottom-flat tubes, bottles produced by Nuc, 25-chamber plates produced by Flow). The main conditions for obtaining positive results was proper selection and composition of growing fluid for cells (MEM with Earle's salts produced by Sigma enriched with vitamins, L-glutamine, amino acids and calf fetal fluid) and limited number of passages of tissue after thawing cells from the bank. With passaging neuroblastoma cells are becoming resistant to infection. Growth of cells abd isolation of the virus were possible both in atmosphere containing 5% CO2 and normal air, but application of a standard incubator required higher usage of growth fluids. Positive results were also obtained with infected material stored for a long time. Storage of material at 37 degrees C was making isolation more difficult. Multiplication of stable viruses on BHK, neuroblastoma and Vero cells permitted for obtaining antigens for serological diagnosis of rabies in vitro.

[Rabies in Poland in 1999]. / Wscieklizna w 1999 roku.

Mass oral vaccination of wild animals against rabies which was introduced in 1993, shows a positive impact on the epizootic situation of rabies in Poland. Sources of wild and domestic animal rabies are present on the northeast part of the country, uncovered by oral vaccination. No cases of rabies in human beings have been reported in Poland in 1999. Out of 6,861 vaccinees, only 1,336 (19%) were bitten by or were in contact with a rabid animals. Main reason of vaccination against rabies were contacts with animals category C (rabies not excluded; 4,782 cases, 69%) or category D (animals healthy during the exposition, 743 cases, 10%.

[Rabies in 1998]. / Wscieklizna w 1998 roku.

Mass oral vaccination of wild animals against rabies which was introduced in 1993, shows a positive impact on the epizootic situation of rabies in Poland. Sources of wild and domestic animal rabies are present on the east part of the country, uncovered by oral vaccination. In parallel to the decreasing number of rabies cases in animals, the number of persons exposed to rabid animals also declines. No case of rabies in human beings has been reported in Poland since 1986. Out of 7172 persons vaccinated in Poland against rabies, only 1408 (19%) were bitten by or were in contact with a rabid animals. Main reason of vaccination against rabies were contacts with animals category C (rabies not excluded)--4989 (70%) or with animals healthy during the exposition (category D)--775 (11%).

[Rabies in Poland in 1997]. / Wscieklizna w Polsce w 1997 roku.

Mass oral vaccination of wild animals against rabies introduced in 1993, shows a positive impact on the epizootic situation of rabies in Poland. In parallel to the decreasing number of rabies cases in animal, the number of persons exposed to rabid parallel animals declines. No case of rabies in human beings has been reported in Poland since 1986. Of 7566 persons vaccinated in Poland against rabies only 1492 (19.7%) were bitten by or were in contact with a rabid animal. Main reason of vaccination against rabies were contact with animals of category C (rabies not excluded) (68.8%) and with animals healthy during the exposition (category D) (11.5%).