[Stable paper indicator systems for rapid identification of microorganisms]. / Stabil'nye bumazhnye indikatornye sistemy dlia uskorennoi identifikatsii mikroorganizmov.

The authors prepared under experimental-industrial conditions paper indicator systems for the express identification of microbes, including carbohydrate discs (by the method of Nikitin et al.), and newly worked out types for the determination of the activity of cytochromoxidase, urease, indol formation, and indicator amino acid decarboxylases (lysin, ornithin, arginine). The use of paper indicator discs proved to be expedient for rationalization of laboratory diagnosis of bacterial intestinal infections.

[Differentiation of bacteria of the genus Aeromonas from other representatives of the Vibrionaceae family on the basis of their DNA]. / Differentsiatsiia bakterii roda Aeromonas ot drugikh predstavitelei semeistva Vibrionaceae na osnove izucheniia ikh DNK.

By comparing the data on the nucleotide composition of DNA, and the phenotypic characteristics, most of the representatives of the genus Aeromonas were clearly differentiated from NAG vibrios isolated in the process of sanitary control of the environment. At the same time some microorganisms with the Aeromonas phenotype, but having a different DNA structure were detected. The use of the method of molecular DNA hybridization indicated that these bacteria were the taxons analogous to the genus Aeromonas.

[A paper indicator system for the identification of Enterobacteria]. / Sistema indikatornaia bumazhnaia dlia identifikatsii énterobakterii.

A paper indicator system has been developed in the USSR for the accelerated identification of vibrios and proposed for the identification of enterobacteria. This system consists of paper discs or strips impregnated with definite substrates (carbohydrates, polyatomic alcohols, amino acids, etc.) in combination with an indicator and stabilized with the polymer coating. Two kits for the identification of enterobacteria to the level of the genus (kit A for 9 tests) and to the level of the species (kit B for 25 tests) are presented.

[Complex studies in the surveillance of the environmental Vibrio flora in the city of Gorki and Gorki Province]. / Kompleksnye issledovaniia pri nadzore za vibriofloroi vneshnei sredy v g. Gor'kom i Gor'kovskoi oblasti.

The analysis of the results of the comprehensive studies (phenotypic and genotypic properties, the precipitation test with the use of spontaneously agglutinating cultures, the determination of the antigenic structure by immunochemical analysis, etc.) carried out during the sanitary microbiological control of the environmental vibrioflora for the last 5 years are presented. Only the combined use of the above-mentioned methods has allowed to ascertain the taxonomic position of atypical cultures.

[Use in laboratory practice of stable paper indicator systems for accelerated differentiation of bacteria of the family Vibrionaceae]. / Primenenie v laboratornoi praktike stabil'nykh bumazhnykh indikatornykh sistem dlia uskorennoi differentsiatsii bakterii semeistva Vibrionaceae.

A total of 5082 cultures belonging to the family Vibrionaceae and 100 strains of enterobacteria were identified by means of the paper indicator systems manufactured by the Gorky Research Institute of Epidemiology and Microbiology and with the use of the classical method. The results thus obtained indicate that these systems proved to be sufficiently sensitive and specific, which allows one to recommend them for wide introduction into laboratory practice.

[An experimental study of a lactoglobulin preparation against opportunistic bacteria and salmonellae]. / Eksperimental'noe izuchenie preparata laktoglobulina protiv uslovno-patogennykh bakterii i sal'monell.

A preclinical study of seven batches of lactoglobulin, a new biological preparation against opportunistic bacteria and salmonellae, has been carried out. High antibacterial activity of the preparation with respect to the virulent forms of Salmonella typhimurium, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, Proteus mirabilis has been established. The preparation has been shown to be safe and nontoxic. The 4-year term of its storage at a temperature of 6 degrees +/- 4 degrees C has been substantiated.