RESUMEN
In this paper, one method was introduced, which was a combination of the cue-related morphine addiction model and a technique for obtaining chronic extracellular recordings of single unit in freely moving rats. With the combination and improvement of this technique, we have successfully applied this new method to study the neuronal activity of the hippocampus CA1 region in morphine withdrawal rats. In all, we found some more accurate and objective cellular characteristics of hippocampal neurons, and considered these characteristics as one of electrophysiological indexes of morphine addiction rats.
Asunto(s)
Electrofisiología/instrumentación , Hipocampo/fisiopatología , Dependencia de Morfina/fisiopatología , Dependencia de Morfina/psicología , Síndrome de Abstinencia a Sustancias/fisiopatología , Potenciales de Acción/fisiología , Animales , Neuronas/fisiología , Ratas , Síndrome de Abstinencia a Sustancias/psicologíaRESUMEN
Recordings of neuronal activity in freely moving rats are common in experiments where electrical signals are transmitted using cables. Such techniques are not common in monkeys because their prehensile abilities are thought to preclude such techniques. However, analysis of brain mechanisms underlying spatial navigation and cognition require the subject to walk. We have developed techniques for recordings in freely moving monkeys in two different situations: a 5 x 5 m testing laboratory and in a 50 m2 open field environment. Neuronal signals are sent to amplifiers and data acquisition systems using cables or telemetry. These techniques provide high quality recordings of single neurons during behaviors such as foraging, walking, and the performance of memory tasks and thus provide a unique opportunity to study primate behavior in a semi-natural situation.