The capture of crude oil droplets by filter feeders at high and low Reynolds numbers.

Crustacean filter feeders capture oil droplets with the use of their ramified appendages. These appendages behave as paddles or sieves, based on the system's Reynolds number. Here, we used high-speed videography, scanning electron microscopy and fluid mechanics to study the capturing mechanisms of crude oil droplets and the filtering appendage's wettability by two species of barnacles (Balanus glandula and Balanus crenatus) and of the freshwater cladoceran Daphnia magna. Our results show that barnacle appendages behave as paddles and capture droplets in their boundary layers at low Reynolds number. At high Reynolds number, droplets are most likely to be captured via direct interception. There is an intermediate range of Reynolds number where droplets can be captured by both mechanisms at the same time. Daphnia magna captures droplets in the boundary layers of the third and fourth pair of thoracic legs with a metachronal motion of the appendages. All studied surfaces were revealed to be highly lipophobic, demonstrating captured oil droplets with high contact angles. We also discuss implications of such capture mechanisms and wettability on potential ingestion of crude oil by filter feeders. These results further our understanding of the capture of crude oil by filter feeders, shedding light on the main entry point of oil in marine food webs.

Using bioluminescence as a tool for studying diversity in marine zooplankton and dinoflagellates: an initial assessment.

Bioluminescence is light chemically produced by an organism. It is widespread across all major marine phyla and has evolved multiple times, resulting in a high diversity of spectral properties and first flash kinetic parameters (FFKP). The bioluminescence of a system is often a good proxy for planktonic biomass. The species-specific parameters of bioluminescent displays can be measured to identify species in situ and describe planktonic biodiversity. Most bioluminescent organisms will flash when mechanically stimulated i.e., when subjected to supra-threshold levels of shear stress. Here we compare first flash kinetic parameters such as flash duration, peak intensity, rise time, decay time, first-flash mechanically stimulated light and e-folding time obtained with the commercially available Underwater Bioluminescence Assessment Tool (UBAT). We provide descriptions of the first flash kinetic parameters of several species of dinoflagellates Pyrocystis fusiformis, Pyrocystis noctiluca, Pyrodinium bahamense, Lingulodinium polyedra, Alexandrium monilatum and two zooplankton (the ctenophore Mnemiopsis leidyi and the larvacean Oikopleura sp.). FFKPs are then compared and discussed using non-parametric analyses of variance (ANOVAs), hierarchical clustering and a linear discriminant analysis to assess the ability to use bioluminescence signatures for identification. Once the first flash kinetic parameters of a bioluminescent species have been described, it is possible to detect its presence using emissions collected by in situ bathyphotometers. Assessing abundance and diversity of bioluminescent species may therefore be possible.

The loss of crude oil droplets by filter feeders and the role of surfactants.

Various methods of oil spill remediation exist, e.g., floating booms, controlled burning and the release of chemical surfactants. These surfactants facilitate the breakup of the slick into micron-sized droplets. Here, we studied the impact such a surfactant has on the size distribution of oil droplets in the water column and in the gut of the filter feeder Daphnia magna. We also studied the effect of surfactants on detachment conditions of chemically and mechanically dispersed oil (respectively MDO and CDO) droplets from capture fibers. Our results show that including solubilized dioctyl sulfosuccinate sodium salt in the mixing of the emulsion produces smaller droplets and a narrower size distribution in the water. In the gut, the size of ingested droplets does not change whether the oil is mixed mechanically or chemically. Also, surfactant coated droplets detach at a lower velocity than mechanically dispersed droplet because of their lower oil/water interfacial tension.

The interactions of oil droplets with filter feeders: A fluid mechanics approach.

Filter feeding animals capture and lose oil droplets using cilia or ramified appendages. Here we demonstrate that copepod and barnacle appendages capture fish, canola and 1-decanol oil droplets up to 11µm without selectivity for size, chemistry, density, viscosity, or interfacial tension. Following capture, the droplets are ingested or lost via detachment. Capture and detachment did not differ between a barnacle appendage and stainless-steel wires of radii Rf=50 and 250µm. Key parameters to detachment include the ratio of oil droplet radius to fiber radius, and the Weber number. Smaller oil droplet size to fiber size ratio r=Ro∕Rf, required a higher We for detachment. These data plot as a curve that predicts whether a droplet will remain captured or detach and re-enter the fluid stream, based on the fluid, the droplet radius to fiber radius ratio, and the oil droplet properties. Significantly, this curve may be used to plan responses to oil spills in marine environments.

The mechanisms of filter feeding on oil droplets: Theoretical considerations.

Filter feeding animals capture food particles and oil droplets from the fluid environment using cilia or appendages composed of arrays of fibers. Here we review the theoretical models that have provided a foundation for observations on the efficiency of particle capture. We then provide the mathematical theoretical framework to characterize the efficient filtration of oil droplets. In the aquatic and marine environments oil droplets are released from the decay of organisms or as hydrocarbons. Droplet size and flow velocity, oil-to-water viscosity ratio, oil-water interfacial tension, oil and water density difference, and the surface wettability, or surface texture, of the filter fiber are the key parameters for oil droplet capture. Following capture, capillary force maintains the droplet at its location due to the oil-water interfacial tension. If the oil-coated fiber is subject to any external force such as viscous or gravitational forces, it may deform and separate from the fiber and re-enter the fluid stream. We show oil droplet capture in Daphnia and the barnacle Balanus glandula, and outline some of the ecological unknowns regarding oil capture in the oceans. Awareness of these mechanisms and their interrelationships will provide a foundation for investigations into the efficiency of various modes of filter feeding on oil droplets.