RESUMEN
OBJECTIVES: To investigate whether and how the long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) sponges microRNA-96 (miR-96) to achieve the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). METHODS: Protein levels were detected by Western blot. Mineralized bone matrix formation was studied by alizarin red staining. Metastasis-associated lung adenocarcinoma transcript 1, miR-96, and osteogenesis-related Messenger RNA expression was assessed by Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). The interactions between miR-96 and osterix (Osx), MALAT1, and miR-96 were determined by luciferase reporter assay. RESULTS: The expression of MALAT1 was upregulated whereas that of miR-96 was downregulated in osteogenic hBMSCs. In addition, the expression of MALAT1 significantly decreased whereas that of miR-96 increased in the hBMSCs of osteoporosis (OP) patients. qRT-PCR and alizarin red staining assays showed that MALAT1 silencing or miR-96 overexpression inhibits hBMSC osteogenic differentiation and vice versa. overexpression of miR-96 reversed the promotive effect of MALAT1 on the osteogenic differentiation of hBMSCs. Dual luciferase report assay verified that miR-96 is a regulatory target of MALAT1 and that Osx is a gene target of miR-96. CONCLUSIONS: Taken together, the results demonstrate that MALAT1 promotes the osteogenic differentiation of hBMSCs by regulating the miR-96/Osx axis. Our study provides novel mechanistic insights into the critical role of lncRNA MALAT1 as a microRNA sponge in OP patients and sheds new light on lncRNA-directed diagnostics and therapeutics in OP.
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Células Madre Mesenquimatosas , MicroARNs , Osteoblastos , Osteoporosis , ARN Largo no Codificante , Factor de Transcripción Sp7 , Médula Ósea , Diferenciación Celular/genética , Células Cultivadas , Humanos , Células Madre Mesenquimatosas/citología , MicroARNs/genética , Osteoblastos/citología , Osteogénesis/genética , ARN Largo no Codificante/genética , Factor de Transcripción Sp7/genéticaRESUMEN
OBJECTIVE: To evaluate the practicability of combining prostate health index (PHI) and multiparametric magnetic resonance imaging (mpMRI) for the detection of clinically significant prostate cancer (csPC) in an Asian population. PATIENTS AND METHODS: We prospectively enrolled patients who underwent prostate biopsy due to elevated serum prostate-specific antigen (PSA > 4 ng/mL) and/or abnormal digital rectal examination in a tertiary referral center. Before prostate biopsy, the serum samples were tested for PSA, free PSA, and p2PSA to calculate PHI. Besides, mpMRI was performed using a 3-T scanner and reported in the Prostate Imaging Reporting and Data System version 2 (PI-RADS v2). The diagnostic performance of PHI, mpMRI, and combination of both was assessed. RESULT: Among 102 subjects, 39 (38.2%) were diagnosed with PC, including 24 (23.5%) with csPC (Gleason ≥ 7). By the threshold of PI-RADS ≥ 3, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) to predict csPC were 100%, 44.9%, 35.8%, and 100%, respectively. By the threshold of PHI ≥ 30, the sensitivity, specificity, PPV, and NPV to predict csPC were 91.7%, 43.6%, 33.3%, and 94.4%, respectively. The area under the receiver operator characteristic curve of combining PHI and mpMRI was greater than that of PHI alone (0.873 vs. 0.735, p = 0.002) and mpMRI alone (0.873 vs. 0.830, p = 0.035). If biopsy was restricted to patients with PI-RADS 5 as well as PI-RADS 3 or 4 and PHI ≥ 30, 50% of biopsy could be avoided with one csPC patient being missed. CONCLUSION: The combination of PHI and mpMRI had higher accuracy for detection of csPC compared with PHI or mpMRI alone in an Asian population.
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Calicreínas/sangre , Imágenes de Resonancia Magnética Multiparamétrica , Antígeno Prostático Específico/sangre , Próstata/diagnóstico por imagen , Neoplasias de la Próstata/sangre , Neoplasias de la Próstata/diagnóstico , Anciano , Pueblo Asiatico , Biopsia , Estudios de Factibilidad , Humanos , Masculino , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Estudios Prospectivos , Neoplasias de la Próstata/diagnóstico por imagen , Sensibilidad y EspecificidadRESUMEN
This paper aimed to investigate whether psoralen inhibits the differentiation and bone resorption by regulating CD4+T cell differentiation in RANKL-induced osteoclastogenesis in RAW264.7 cells, and elucidate its mechanism for osteoporosis. CD4+T cells were isolated from spleen cells of Balb/c mice by immunomagnetic separation method. The cells were divided into blank control group and psoralen group. The cells were cultured in 24-well plates and cultured for 3 days, and then they were collected for co-culture experiments after 4 days. Co-culture experiments were divided into RAW264.7 cell group, psoralen+RAW264.7 cell group, without psoralen treatment of CD4+T cells+RAW264.7 cell group, psoralen treatment of CD4+T cells+RAW264.7 cell group. After 5 days of co-culture, TRAP staining was used to detect the number of osteoclasts, and after 8 days of co-culture, bone resorption was evaluated by toluidine blue staining. The expressions of RORγt, Foxp3, IL-17, TNF-α, TGF-ß and IL-10 in CD4+T cells and osteoclast differentiation-related genes MMP-9, TRAP and Cat-K were detected by Real-time polymerase chain reaction (RT-PCR); ELISA kit was used to detect IL-17, TNF-α, TGF-ß and IL-10 and other cytokines levels. Our data confirmed that the psoralen significantly promoted the expression of Foxp3, TGF-ß and IL-10 in CD4+T, and inhibited the expression of RORγt, IL-17 and TNF-α in CD4+T, the CD4+T cells without treatment by psoralen can significantly promote RANKL-induced differentiation of RAW264.7 to osteoclasts, and psoralen treatment of CD4+T can significantly inhibit RANKL-induced RAW264.7 osteoclast differentiation and bone resorption. Taken together, psoralen inhibits the differentiation and bone resorption of RAW264.7 into osteoclasts by promoting the development of CD4+ CD25+ Treg/Th17 balance in CD4+T cells to CD4+CD25+T.
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Resorción Ósea , Linfocitos T CD4-Positivos/citología , Diferenciación Celular/efectos de los fármacos , Ficusina/farmacología , Osteoclastos/efectos de los fármacos , Animales , Ratones , Ratones Endogámicos BALB C , Ligando RANK , Células RAW 264.7RESUMEN
The dextranase added in current commercial dextranase-containing mouthwashes is largely from fungi. However, fungal dextranase has shown much higher optimum temperature than bacterial dextranase and relatively low activity when used in human oral cavities. Bacterial dextranase has been considered to be more effective and suitable for dental caries prevention. In this study, a dextranase (Dex410) from marine Arthrobacter sp. was purified and characterized. Dex410 is a 64-kDa endoglycosidase. The specific activity of Dex410 was 11.9 U/mg at optimum pH 5.5 and 45 °C. The main end-product of Dex410 was isomaltotriose, isomaltoteraose, and isomaltopentaose by hydrolyzing dextran T2000. In vitro studies showed that Dex410 effectively inhibited the Streptococcus mutans biofilm growth in coverage, biomass, and water-soluble glucan (WSG) by more than 80, 90, and 95 %, respectively. The animal experiment revealed that for short-term use (1.5 months), both Dex410 and the commercial mouthwash Biotene (Laclede Professional Products, Gardena, CA, USA) had a significant inhibitory effect on caries (p = 0.0008 and 0.0001, respectively), while for long-term use (3 months), only Dex410 showed significant inhibitory effect on dental caries (p = 0.005). The dextranase Dex410 from a marine-derived Arthrobacter sp. strain possessed the enzyme properties suitable to human oral environment and applicable to oral hygiene products.
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Arthrobacter/enzimología , Caries Dental/tratamiento farmacológico , Dextranasa/metabolismo , Dextranasa/farmacología , Animales , Organismos Acuáticos/enzimología , Biopelículas/efectos de los fármacos , Caries Dental/prevención & control , Dextranasa/uso terapéutico , Femenino , Datos de Secuencia Molecular , Ratas Wistar , Streptococcus mutans/efectos de los fármacos , Streptococcus mutans/fisiologíaRESUMEN
In this study, the fibroblasts cell line derived from ear marginal tissue of Yunnan semi-fine wool sheep was successfully established using the primary explants technique and cryopreservation technology. Additionally, the protective effect of synthetic ice blocker (SIB) including 1, 3-cyclohexanediol (1, 3-CHD) and 1, 4-cyclohexanediol (1, 4-CHD) on frozen fibroblast cells was also assessed and compared. Propidium iodide (PI) was used to stain the dead cells following cryopreservation and thawing. The results showed that compared with Medium 199 (M199) and Dulbecco's modified Eagle's medium : Nutrient Mixture F-12 (1 : 1) Mixture (DMEM/F12), Dulbecco's modified Eagle's medium (DMEM) may be more suitable for the primary culture of fibroblast cells of Yunnan semi-fine wool sheep. The growth curve of cells is a typical "S" type. After subculture for four days, the cells entered the plateau phase and began to degenerate. Biological analysis showed that the population doubling time (PDT) for subculturing fibroblast cells was approximately 26h. The Karyotyping data indicated that the percentage of fibroblast cells with normal chromosome number 2n = 54 was over 90% following subculture for 10 passages. Moreover, the tests for bacteria, fungi, viruses and mycoplasma were negative. After serial subculture for 5 generations, the fibroblast cells were cryopreserved in the presence or absence of 1, 3-CHD or 1, 4-CHD. The data indicated that with increase of the synthetic ice blocker concentrations, the viability of frozen-thawed fibroblast cells was firstly increased and then decreased. When the concentration of 1, 3-CHD or 1, 4-CHD was 50 mM, the viable percentage of frozen-thawed fibroblast cells was 91.93% +/- 2.24% and 94.13% +/- 0.55% respectively and significantly higher than that of the cells frozen in the absence of synthetic ice blockers (88.10% +/- 1.49%, P < 0.05). In conclusion, the skin fibroblast cell line of Yunnan semi-fine wool sheep was firstly established in this study. Additionally, the presence of synthetic ice blocker can increase the viability of frozen-thawed sheep fibroblast cell line.
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Criopreservación/veterinaria , Fibroblastos/citología , Hielo/análisis , Ovinos , Animales , Técnicas de Cultivo de Célula , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Criopreservación/métodos , Crioprotectores/metabolismo , Medios de Cultivo/metabolismo , Ciclohexanoles/metabolismo , Fibroblastos/metabolismo , Cariotipificación , Ovinos/genética , Ovinos/metabolismoRESUMEN
OBJECTIVE: Lipopolysaccharide-induced tumor necrosis factor-α factor (LITAF) protein is a newly discovered inflammatory protein. This study aims to study the role of LITAF in the formation of atherosclerosis. METHODS: A total of 10 C57BL/6J mice and 10 C57BL/6J mice with knockout of LITAF gene (C57BL/6J-LITAF-) were divided into two groups: the control group and the LITAF-/- group. The animals were accommodated for 16 weeks and then euthanized with their hearts and aortas isolated thereafter. Next, the roots of the mouse aorta were cryosectioned and stained with Oil Red O staining and immunohistochemical staining (CD68, α-SMA, and Masson), respectively. The area of Oil Red O staining and the proportion of positive expression after immunohistochemical staining were then compared between the control and LITAF-/- groups. At the same time, the blood of mice was collected for the extraction of proteins and RNA. The proteins and RNA were used to detect the expression of major molecules of the NF-κB inflammatory pathway in mice in the control group and the LITAF-/- group by Western blotting and RT-PCR. RESULTS: Oil Red O staining of the aortic root sections of the mice in each group revealed that the area of atherosclerotic plaques in the LITAF-/- group was substantially lower than that in the control group (P<0.05). Moreover, immunohistochemical staining determined that the expression level of α-SMA and CD68 in the LITAF-/- group was significantly lower than that in the control group, whereas the results were reversed following Masson staining (P<0.05). The expression levels of P65 and caspase 3 were significantly lower in the LITAF-/- group than in the control group (P<0.05), whereas the expression level of IκB was higher in the LITAF-/- group. CONCLUSION: LITAF might participate in the formation of atherosclerotic plaque through the NF-κB pathway and play a promoting role in the formation of atherosclerosis.
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Aterosclerosis , Placa Aterosclerótica , Animales , Ratones , Aterosclerosis/metabolismo , Lipopolisacáridos , Ratones Endogámicos C57BL , FN-kappa B/genética , FN-kappa B/metabolismo , Placa Aterosclerótica/genética , Placa Aterosclerótica/patología , ARN , Transducción de Señal , Factor de Necrosis Tumoral alfaRESUMEN
BACKGROUND: Severe acute pancreatitis (SAP) is characterized by fatal pathogenic conditions and a high mortality. It is important to study SAP complicated with multiple organ injury. In this study we compared the protective effects of three traditional Chinese medicines (Ligustrazine, Kakonein and Panax Notoginsenoside) on the small intestine and immune organs (thymus, spleen and lymph nodes) of rats with SAP and explored their mechanism of action. METHODS: One hundred forty-four rats with SAP were randomly divided into model control, Ligustrazine-treated, Kakonein-treated, and Panax Notoginsenoside-treated groups (n=36 per group). Another 36 normal rats comprised the sham-operated group. According to the different time points after operation, the experimental rats in each group were subdivided into 3-, 6- and 12-hour subgroups (n=12). At various time points after operation, the mortality rate of rats and pathological changes in the small intestine and immune organs were recorded and the serum amylase levels were measured. RESULTS: Compared to the model control groups, the mortality rates in all treated groups declined and the pathological changes in the small intestine and immune tissues were relieved to different degrees. The serum amylase levels in the three treated groups were significantly lower than those in the model control group at 12 hours. The pathological severity scores for the small intestinal mucosa, thymus and spleen (at 3 and 12 hours) in the Ligustrazine-treated group, for the thymus (at 3 and 12 hours) and spleen (at 3 and 6 hours) in the Kakonein-treated group, and for the thymus (at 3 hours) and spleen (at 3 hours) in the Panax Notoginsenoside-treated group were significantly lower than those in the model control group. The pathological severity scores of the small intestinal mucosa (at 6 and 12 hours) and thymus (at 6 hours) in the Ligustrazine-treated group were significantly lower than those in the Kakonein- and Panax Notoginsenoside-treated groups. CONCLUSIONS: All the three traditional Chinese drugs significantly alleviated the pathological changes in the small intestine and immune organs of SAP rats. Ligustrazine was the most effective one among them.
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Medicamentos Herbarios Chinos/farmacología , Inmunidad/efectos de los fármacos , Intestino Delgado/efectos de los fármacos , Isoflavonas/farmacología , Tejido Linfoide/efectos de los fármacos , Pancreatitis Aguda Necrotizante/prevención & control , Pirazinas/farmacología , Animales , Apoptosis/efectos de los fármacos , Modelos Animales de Enfermedad , Intestino Delgado/inmunología , Intestino Delgado/patología , Ligusticum , Tejido Linfoide/inmunología , Tejido Linfoide/patología , Pancreatitis Aguda Necrotizante/inmunología , Pancreatitis Aguda Necrotizante/patología , Ratas , Ratas Sprague-Dawley , Índice de Severidad de la Enfermedad , Vasodilatadores/farmacologíaRESUMEN
RATIONALE: Radical cystectomy and urinary diversion remains the definite management for muscle invasive bladder urothelial cancer. Internal herniation caused by ureteral adhesion is an extremely rare complication after the procedure. To the best of our knowledge, this is the first case report of small bowel obstruction and internal herniation occurring between bilateral ureters and urinary diversion after robot-assisted radical cystectomy (RARC). PATIENT CONCERNS: A 64-year-old woman presented with symptom of small bowel obstruction such as nausea, vomiting, and abdominal fullness after RARC and Indiana pouch. Another 61-year-old man presented with left obstructive hydronephrosis and recurrent pyelonephritis after RARC and ileal conduit. DIAGNOSIS: Both patients received computed tomography scans and the results were suggestive of small bowel herniation between bilateral ureters and urinary diversion. INTERVENTIONS: The 2 patients underwent open ureterolysis and internal hernia reduction. During the operation, bowel loop herniation between the interureteral spaces were found. OUTCOMES: Both patients recovered smoothly after second operation. LESSONS: The incidence of internal herniation may increase by the growing use of RARC. Suitable stoma position, appropriate length of ureter dissection, and retroperitonealization can help prevent this complication.
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Cistectomía/efectos adversos , Hernia/etiología , Complicaciones Posoperatorias/etiología , Procedimientos Quirúrgicos Robotizados/efectos adversos , Enfermedades Ureterales/etiología , Derivación Urinaria/efectos adversos , Cistectomía/métodos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Procedimientos Quirúrgicos Robotizados/métodos , Derivación Urinaria/métodosRESUMEN
The association of microRNA (miRNA) with tumor has gradually become an active medical research field, since its discovery in 1993. The aim of the present study was to clarify how microRNA16 expression affects the proliferation and survival of pituitary tumor, revealing its potential mechanism. MicroRNA16 expression of pituitary tumor patients was observably declined, compared with the normal group. A high expression of microRNA16 showed longer survival in pituitary tumor patients, compared to a low expression of microRNA16 in pituitary tumor patients. MicroRNA16 upregulation effectively decreased cell proliferation and induced apoptosis in HP75 cells. MicroRNA16 overexpression effectively induced p27, Bax protein expression and caspase3/8 activities, and suppressed phosphorylation-(p)-p38, NFκB, MMP9 and VEGFR2 protein expression in HP75 cells. After VEGFR2 suppression, the effects of microRNA16 overexpression on cell proliferation and apoptosis were significantly inhibited in HP75 cells. Moreover, the effects of microRNA16 overexpression on p27, Bax protein expression and caspase3/8 activities were significantly decreased in HP75 cells after p38 suppression. VEGFR2 or NFκB suppression reduced the effects of microRNA16 overexpression on pp38, NFκB, MMP9 and VEGFR2 protein expression inhibition in HP75 cells. Our results suggest that microRNA16 expression affects the proliferation and angiogenesis of pituitary cancer through the VEGFR2/p38/NFκB signaling pathway.
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MicroARNs/metabolismo , Neoplasias Hipofisarias/metabolismo , Transducción de Señal , Apoptosis , Caspasas/metabolismo , Línea Celular Tumoral , Proliferación Celular , Humanos , Metaloproteinasa 9 de la Matriz/metabolismo , FN-kappa B/metabolismo , Neoplasias Hipofisarias/enzimología , Neoplasias Hipofisarias/genética , Neoplasias Hipofisarias/patología , Receptor 2 de Factores de Crecimiento Endotelial Vascular/metabolismo , Proteína X Asociada a bcl-2/metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Contagious pustular dermatitis is an exanthematous zoonotic disease caused by the orf virus. Pandemic outbreaks of this disease cause great economic losses, while the pathogenesis of this disease still remains obscure. In this study, blood samples were collected from 628 asymptomatic goats across China for PCR-based virus detection. We detected the orf virus in the blood of asymptomatic goats. Moreover, the orf virus obtained from the blood of infected goats was infectious and induced typical symptoms of contagious pustular dermatitis after inoculation of uninfected dairy goats. In summary, our data provide evidence that asymptomatic animals may be carriers of orf virus. Our findings should contribute to elucidating the details underlying the pathogenesis of contagious pustular dermatitis.
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Ectima Contagioso/sangre , Ectima Contagioso/virología , Enfermedades de las Cabras/virología , Virus del Orf/aislamiento & purificación , Virus del Orf/patogenicidad , Animales , Enfermedades Asintomáticas/epidemiología , China/epidemiología , Brotes de Enfermedades/veterinaria , Ectima Contagioso/patología , Ectima Contagioso/transmisión , Enfermedades de las Cabras/epidemiología , Cabras/virología , Virus del Orf/genética , Filogenia , Reacción en Cadena de la Polimerasa , VirulenciaRESUMEN
PURPOSE: Silicone rubber implants have been widely used to repair soft tissue defects and deformities. However, poor biocompatibility can elicit capsule formation, usually resulting in prosthesis contracture and displacement in long-term usage. To overcome this problem, this study investigated the properties of silicone rubber materials with or without a microgroove-patterned surface and with or without carbon (C)-ion implantation. MATERIALS AND METHODS: Atomic force microscopy, X-ray photoelectron spectroscopy, and a water contact angle test were used to characterize surface morphology and physicochemical properties. Cytocompatibility was investigated by a cell adhesion experiment, immunofluorescence staining, a Cell Counting Kit-8 assay, and scanning electron microscopy in vitro. Histocompatibility was evaluated by studying the inflammatory response and fiber capsule formation that developed after subcutaneous implantation in rats for 7 days, 15 days, and 30 days in vivo. RESULTS: Parallel microgrooves were found on the surfaces of patterned silicone rubber (P-SR) and patterned C-ion-implanted silicone rubber (PC-SR). Irregular larger peaks and deeper valleys were present on the surface of silicone rubber implanted with C ions (C-SR). The silicone rubber surfaces with microgroove patterns had stable physical and chemical properties and exhibited moderate hydrophobicity. PC-SR exhibited moderately increased dermal fibroblast cell adhesion and growth, and its surface microstructure promoted orderly cell growth. Histocompatibility experiments on animals showed that both the anti-inflammatory and antifibrosis properties of PC-SR were slightly better than those of the other materials, and there was also a lower capsular contracture rate and less collagen deposition around implants made from PC-SR. CONCLUSION: Although the surface chemical properties, dermal fibroblast cell growth, and cell adhesion were not changed by microgroove pattern modification, a more orderly cell arrangement was obtained, leading to enhanced biocompatibility and reduced capsule formation. Thus, this approach to the modification of silicone rubber, in combination with C-ion implantation, should be considered for further investigation and application.
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Materiales Biocompatibles/química , Carbono/química , Prótesis e Implantes , Elastómeros de Silicona/química , Animales , Cápsulas , Adhesión Celular , Línea Celular , Colágeno/química , Femenino , Fibroblastos/citología , Humanos , Interacciones Hidrofóbicas e Hidrofílicas , Inflamación , Iones/química , Microscopía de Fuerza Atómica , Microscopía Fluorescente , Espectroscopía de Fotoelectrones , Implantación de Prótesis , Ratas , Ratas Sprague-Dawley , Propiedades de Superficie , Agua/químicaRESUMEN
This study aimed to analyze the expression and clinical significance of cyclin G2 (CCNG2) in thyroid carcinoma and the biological effects of CCNG2 overexpression in a cell line. Immunohistochemistry and Western blotting were used to analyze CCNG2 protein expression in 63 cases of thyroid cancer and normal tissues to allow the relationship with clinical factors to be assessed. CCNG2 lentiviral and empty vectors were transfected into the thyroid cancer K1 cell line. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were applied to detect the mRNA and protein levels of CCNG2. MTT assay and cell cycle were also conducted to assess the influence of up-regulated expression of CCNG2 on K1 cell biology. The level of CCNG2 protein expression was found to be significantly lower in thyroid cancer tissue than normal tissues (P<0.05). Western blot: The relative amount of CCNG2 protein in thyroid cancer tissue was respectively found to be significantly lower than in normal tissues (P<0.05), correlating with lymph node metastasis, clinic stage and histological grade (P<0.05), but not gender, age or tumor size (P>0.05). Loss of CCNG2 expression correlated significantly with poor overall survival time on Kaplan-Meier analysis (P<0.05). The results for biological functions showed that K1 cell transfected CCNG2 had a lower survival fraction, a greater percentage in the G0/G1 phases, and lower cyclin-dependent kinase 2 (CDK2) protein expression compared with K1 cells non-transfected with CCNG2 (P<0.05). CCNG2 expression decreased in thyroid cancer and correlated significantly lymph node metastasis, clinic stage, histological grade and poor overall survival, suggesting that CCNG2 may play important roles as a negative regulator in thyroid cancer K1 cells by promoting degradation of CDK2.
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Adenocarcinoma/metabolismo , Ciclina G2/metabolismo , Quinasa 2 Dependiente de la Ciclina/metabolismo , Glándula Tiroides/metabolismo , Neoplasias de la Tiroides/metabolismo , Adenocarcinoma/genética , Adenocarcinoma/secundario , Adulto , Anciano , Apoptosis , Western Blotting , Proliferación Celular , Ciclina G2/genética , Quinasa 2 Dependiente de la Ciclina/genética , Femenino , Citometría de Flujo , Humanos , Técnicas para Inmunoenzimas , Metástasis Linfática , Masculino , Persona de Mediana Edad , Proteolisis , ARN Mensajero/genética , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Neoplasias de la Tiroides/genética , Neoplasias de la Tiroides/patología , Células Tumorales CultivadasRESUMEN
OBJECTIVE: Based on suspected pulmonary nodule segmentation images obtained previously and with a large-sample training, automatic detection and diagnosis of the pulmonary nodules on CT images was realized by extracting the multi-dimensional features of the pulmonary nodule images and the application of LDA and SVM statistical classifiers. Experimental results showed that this detection and diagnosis method produced better classification results, and is practical for application in CAD systems.
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Interpretación de Imagen Asistida por Computador/métodos , Procesamiento de Imagen Asistido por Computador , Nódulo Pulmonar Solitario/diagnóstico por imagen , Tomografía Computarizada por Rayos X/métodos , Humanos , Modelos Lineales , Máquina de Vectores de SoporteRESUMEN
After spraying different concentrations of two brands pesticide omethoate on cole (Brassica campestris L.) leaves, the leaf chlorophyll a fluorescence transients were measured by a Plant Efficiency Analyzer (PEA), and the toxicological effects and rudimental dynamic courses of omethoate on the leaf photosystem II (PS II) were investigated by JIP-test. The results showed that after spraying omethoate except at the concentration of 0.50% , the maximal efficiency of photochemistry (F(v)/F(m)) did not have a remarkable change. However, with increasing omethoate concentration, the minimal fluorescence F(o), maximal fluorescence F(m), relative variable fluorescence at the J-step (V(J)), and electron transport flux perreactive center in PS II (ET(o)/RC) increased remarkably, but psi(o), the efficiency that a trapped exciton in PS ]I moved an electron into the electron transport chain beyond Q(A)-, decreased remarkably. The test two brands of pesticide omethoate had almost alike effects on the PS II of cole, and the residual effect of the pesticide was the strongest at the third day after spraying and petered out from the ninth to twelfth day. The main targets of omethoate on the PS II of cole could be listed as promoting the reduction from Q(A) to Q(A) (-) (increasing of V(J)) and the electron transmission from Q(A) (-) to Q(B) (increasing of ET(o)/RC).