[Application of digital pathology tools. An unusual case of non-Hodgkin lymphoma]. / Anwendung von Verfahren der digitalen Pathologie. Fallbeispiel eines ungewöhnlichen Non-Hodgkin-Lymphoms.

Currently, lymphoma diagnosis is based on a combination of morphology, immunophenotyping, and molecular testing. Using the example of an unusual case of malignant non-Hodgkin lymphoma, we show that improved visualization using digital pathology contributes to the convergence of these complementary diagnostic modalities. A 45-year-old woman presented with skin rash and cervical lymphadenopathy. Histological workup of an excised lymph node showed loss of normal architecture with diffuse infiltration and increased mitotic activity. Immunohistochemistry for CD3/CD5 showed atypical arrangement and infiltration of a T-cell population that dominated over regionally dense, MUM1-positive plasmacellular infiltrates. Expanded CD21/CD23-positive meshworks of follicular dendritic cells were present within and between regressed follicles and the T-cell infiltrate; staining for CD56 and cyclin-D1 was negative. Quantification of Ki-67 staining within the T-, B- and plasmacellular compartments was achieved by digital image conversion, overlay and subsequent quantification algorithms that revealed proliferation within more than 60% of T-cells, over 50% of plasma cells and only 20% of B-cells. Clonality analysis by PCR revealed monoclonal rearrangement for both T-cell receptor gamma chains and immunoglobulin heavy chains. Taken together, we present an unusual combination of an angioimmunoblastic T-cell lymphoma (AITL) and simultaneous plasmacellular lymphoma. This report demonstrates how application of modern tools of digital pathology can visually integrate unusual morphological and molecular findings.

Differential improvements from haloperidol in two types of schizophrenics.

Two types of schizophrenic patients, 27 with a high and 21 with a low energetic level, were treated for 3 weeks under double blind conditions with 16 (24 patients) or 80 mg (24 patients) haloperidol orally per day. The patients were rated by the ward psychiatrist as markedly or not markedly improved. Evaluation made by odds ratio comparison showed that (1) patients with a high energetic level (plus-type patients) improved with low doses whereas (2) patients with a low energetic level (minus-type patients) improved with high doses of haloperidol.

Defining tranquilizers operationally by non additive effect in experimental stress situations.

Clinically and theoretically, a tranquilizer should be more effective under stress than under non stress conditions. By transformation into a statistical non additivity equivalent, a 2 X 3 factorial design was derived with a drug factor (Cloxazolam as a tranquilizer) and a social stress factor. The results in healthy young subjects show a significant drug- x -stress interaction, indicating the non additivity of both treatments. Psychological interpretations, according to Yerkes-Dodson law, and therapeutic and experimental conclusions are added.

Differential drug effects identified by 3-way configural frequency analysis.

48 acute paranoic schizophrenics were treated by high or low dosages of haloperidol and rated for improvement after 3 weeks of treatment. The treatment effect was a differential one in that so-called plus-type schizophrenics improved from low dosages of haloperidol while so-called minus-type schizophrenics improved from high dosages of haloperidol. Testing for differential effects was made by 3-way configural frequency analysis.

[Syndrome assessment using a generalized interaction structure analysis]. / Syndromaufklärung mittels generalisierter Interaktionsstrukturanalyse.

The concept of conventional interaction-structure-analysis (ISA) consists of comparing and analyzing two groups of variables. In the present paper this concept is thus generalized that more than two groups of variables can be analyzed. Data from depression research are analyzed by forming three groups of symptoms. The discussion expounds on strategies of data analysis with generalized ISA (GISA).

[Configuration cluster analysis as an alternative to configuration frequency analysis]. / Die Konfigurations-Cluster-Analyse als Alternative zur KFA.

The paper discusses configural cluster analysis (CCA) as an alternative to configural frequency analysis (CFA). CFA defines types as deviations from the assumption of no interactions among the variables. CCA defines clusters as deviations from the stronger assumption of a total lack of effects, in the contingency table. Variations of CCA as aggregating CCA, hierarchical CCA, m-sample CCA, and CCA of profile shifts are discussed. An example analyzes data from research on depression.

[Comparison of k therapies after determination of success and failure]. / Vergleich von k Behandlungen nach Erfolgs-und Misserfolgseurteilung.

To compare k therapeutic methods, N patients are suggested to be assigned to k therapies at random. The effect of each therapy is rated to be present or absent. The resulting k x 2 contingency table is evaluated according to one of the following questions: 1) Do the k therapies differ in their effects? 2) Is a given therapy more effective than the k-1 remaining therapies? 3) Is a given therapy more effective than a competitive therapy? The methods are exemplified by a numerical example from treatments of schizophrenics.