RESUMEN
BACKGROUND: Bacillomycin D-C16 can induce resistance in cherry tomato against pathogens; however, the underlying molecular mechanism is poorly understood. Here, the effect of Bacillomycin D-C16 on induction of disease resistance in cherry tomato was investigated using a transcriptomic analysis. RESULTS: Transcriptomic analysis revealed a series of obvious enrichment pathways. Bacillomycin D-C16 induced phenylpropanoid biosynthesis pathways and activated the synthesis of defense-related metabolites including phenolic acids and lignin. Moreover, Bacillomycin D-C16 triggered a defense response through both hormone signal transduction and plant-pathogen interactions pathways, and increased the transcription of several transcription factors (e.g., AP2/ERF, WRKY and MYB). These transcription factors might contribute to the further activated the expression of defense-related genes (PR1, PR10 and CHI) and stimulated the accumulation of H2O2. CONCLUSION: Bacillomycin D-C16 can induce resistance in cherry tomato by activating the phenylpropanoid biosynthesis pathway, hormone signal transduction pathway and plant-pathogen interactions pathway, thus activating comprehensive defense reaction against pathogen invasion. These results provided a new insight into the bio-preservation of cherry tomato by the Bacillomycin D-C16.
Asunto(s)
Solanum lycopersicum , Solanum lycopersicum/genética , Transcriptoma , Resistencia a la Enfermedad/genética , Peróxido de Hidrógeno , Hormonas , Factores de Transcripción/genética , Enfermedades de las Plantas/genéticaRESUMEN
Fusarium verticillioides causes ear and kernel rot in maize and produces mycotoxins, like fumonisin B1 (FB1). Bacillomycin D-C16 is a natural antimicrobial lipopeptide produced by Bacillus subtilis. In this study, the inhibitory effects of Bacillomycin D-C16 on the growth of F. verticillioides and on the production of FB1 in maize were investigated. Bacillomycin D-C16 displayed strong fungicidal activity against F. verticillioides, with a minimum inhibitory concentration (MIC) of 32 g/L. Scanning electron microscopy (SEM) showed that Bacillomycin D-C16 altered the morphology of F. verticillioides mycelia. Bacillomycin D-C16 reduced the ergosterol content, increased the release of nucleic acids and proteins, and increased the levels of reactive oxygen species (ROS) in fungal mycelia. Bacillomycin D-C16 also significantly inhibited the production of FB1 by inhibiting mycelial growth and decreasing the levels of fumonisin biosynthetic genes 1 (fum1), fum6 and fum14. The application of Bacillomycin D-C16 on maize kernels prior to storage inhibited the growth of F. verticillioides and the production of FB1. Our results suggested that Bacillomycin D-C16 has a significant antifungal activity that could be used as a potential natural antimicrobial agent to control food contamination and to ensure food safety.
Asunto(s)
Fumonisinas , Fusarium , Péptidos Catiónicos Antimicrobianos , Zea maysRESUMEN
The soft rot disease caused by Rhizopus stolonifer is an important disease in cherry tomato fruit. In this study, the effect of iturin A on soft rot of cherry tomato and its influence on the storage quality of cherry tomato fruit were investigated. The results showed that 512 µg/mL of iturin A could effectively inhibit the incidence of soft rot of cherry tomato fruit. It was found that iturin A could induce the activity of resistance-related enzymes including phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), glucanase (GLU), and chitinase (CHI), and active oxygen-related enzymes including ascorbate peroxidases (APX), superoxide dismutases (SOD), catalases (CAT), and glutathione reductase (GR) of cherry tomato fruit. In addition, iturin A treatment could slow down the weight loss of cherry tomato and soften the fruit. These results indicated that iturin A could retard the decay and improve the quality of cherry tomato fruit by both the inhibition growth of R. stolonifera and the inducing the resistance.
Asunto(s)
Resistencia a Medicamentos/efectos de los fármacos , Frutas/metabolismo , Péptidos Cíclicos/farmacología , Enfermedades de las Plantas/microbiología , Raíces de Plantas/metabolismo , Solanum lycopersicum/metabolismo , Frutas/microbiología , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Solanum lycopersicum/microbiología , Proteínas de Plantas/biosíntesis , Raíces de Plantas/microbiología , Rhizopus/crecimiento & desarrolloRESUMEN
BACKGROUND: Synthetic fungicides are most commonly used for controlling postharvest disease of fruit, although they can cause the emergence of drug-resistant strains, environmental pollution and fruit safety issues. Bacillomycin D (BD), a novel antifungal lipopeptide, and chitosan (CTS) are applied for the preservation of cherry tomato. RESULTS: The combination of BD and CTS showed an additive inhibition on the growth of Rhizopus stolonifer and Botrytis cinerea compared to that of its individual compound. In addition, BD + CTS reduced the incidence of soft rot and gray mold in cherry tomato caused by R. stolonifer and B. cinerea, respectively. Tomato treated with BD + CTS exhibited a lower weight loss and higher firmness and higher contents of total soluble solids, titratable acidity and ascorbic acid compared to those treated with sterile water (control). The kinetics models demonstrated that the shelf life of cherry tomato treated with BD + CTS could be extended by approximately 15 days longer than the control. CONCLUSION: The utilization of BD + CTS provided a novel strategy for reducing postharvest fungal rot and maintaining the storage quality of cherry tomato. © 2020 Society of Chemical Industry.
Asunto(s)
Péptidos Catiónicos Antimicrobianos/farmacología , Botrytis/efectos de los fármacos , Quitosano/farmacología , Conservación de Alimentos/métodos , Fungicidas Industriales/farmacología , Rhizopus/efectos de los fármacos , Solanum lycopersicum/microbiología , Botrytis/crecimiento & desarrollo , Sinergismo Farmacológico , Frutas/química , Frutas/microbiología , Solanum lycopersicum/química , Enfermedades de las Plantas/microbiología , Rhizopus/crecimiento & desarrolloRESUMEN
Bacillomycin D can inhibit the growth of Aspergillus ochraceus in food samples. In addition, it can induce apoptosis in and inhibit the proliferation of cancer cells, although the details of this mechanism are unknown. In this study, we separated bacillomycin D-C14, D-C15, D-C16 monomers from the Bacillus subtilis strain fmbJ. The bacillomycin D monomers containing longer fatty acid chains better induced apoptosis in Bgc-823, Sgc-7901, and Hgc-27 gastric cancer cells. The Bgc-823 cell line was the most sensitive. Acridine orange-ethidium bromide staining indicated that bacillomycin D-C16-induced Bgc-823 cell death by triggering apoptosis, characterized by membrane blebbing, cellular shrinkage, and DNA fragmentation. Flow cytometric analysis showed a bacillomycin D-C16 dose-dependent trigger of Bgc-823 apoptosis. Bacillomycin D-C16-induced the mitochondrial pathway, as indicated by a reduced Bcl-2/Bax expression ratio, enhanced cytochrome C release, and higher levels of cleaved caspase-3. Furthermore, bacillomycin D-C16 effectively repressed phosphorylation of the serine-threonine protein kinase Akt at Ser-473 and increased the levels of the FoxO3a protein. The combination of the PI3K/Akt-inhibitor BEZ235 with bacillomycin D-C16 enhanced the apoptosis of Bgc-823 cells. Together, these findings indicated that bacillomycin D-C16 induces apoptosis through the PI3K/Akt and FoxO3a signaling pathways.
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Péptidos Catiónicos Antimicrobianos/farmacología , Proteína Forkhead Box O3/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Neoplasias Gástricas/tratamiento farmacológico , Péptidos Catiónicos Antimicrobianos/química , Antineoplásicos/química , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Bacillus subtilis/química , Bacillus subtilis/metabolismo , Línea Celular Tumoral , Humanos , Transducción de Señal/efectos de los fármacos , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologíaRESUMEN
The inhibitory effect of Bacillomycin D, a cyclic lipopeptide, on Rhizopus stolonifer colonization of cherry tomato was studied, and its possible mechanism of action was explored. Bacillomycin D showed a direct inhibitory effect on R. stolonifer spore germination and mycelial growth in vitro. It conferred both a direct inhibitory effect on R. stolonifer growth in cherry tomato in vivo and induced host resistance in cherry tomato. Moreover, Bacillomycin D treatment significantly increased the activities of plant defense-related enzymes, including chitinase (CHI), ß-1,3-glucanase (GLU), phenylalanine ammonia-lyase (PAL), and peroxidase (POD). Real-time PCR (RT-PCR) showed that defense-related genes involved in the salicylic acid defense signaling pathway and genes encoding pathogenesis-related proteins were up-regulated in Bacillomycin D treatment. Furthermore, Bacillomycin D-C16 resulted in direct inhibition and a remarkable induced resistance to R. stolonifer which was higher than as induced by Bacillomycin D-C14. Together, the data indicated that Bacillomycin D can control the growth of R. stolonifer through both the direct inhibition of the fungus and the activation of defense-related genes and enzymes in cherry tomato.
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Péptidos Catiónicos Antimicrobianos/farmacología , Frutas/microbiología , Rhizopus/efectos de los fármacos , Rhizopus/crecimiento & desarrollo , Solanum lycopersicum/microbiología , Quitinasas/metabolismo , Frutas/enzimología , Glucano 1,3-beta-Glucosidasa/metabolismo , Solanum lycopersicum/enzimología , Peroxidasa/metabolismo , Fenilanina Amoníaco-Liasa/metabolismo , Enfermedades de las Plantas/microbiología , Esporas Fúngicas/efectos de los fármacos , Esporas Fúngicas/crecimiento & desarrolloRESUMEN
Cronobacter spp. is an important foodborne pathogen that can cause life-threatening diseases in infants and immunocompromised adults. The present study was carried out to understand the prevalence and characterization of Cronobacter spp. in dried edible mushrooms in Jiangsu province, China. Cronobacter isolates were identified and genotyped by multilocus sequence typing (MLST); the antimicrobial susceptibility of Cronobacter strains was determined by the disk diffusion method; the biofilm formation ability of Cronobacter spp. was assessed using the microtiter plate method. The overall prevalence of Cronobacter spp. in dried edible mushrooms was 14.8%, with the highest contamination rate of after 37.2% found in Auricularia auricular. The Cronobacter isolates were identified as C. sakazakii (n = 26), C. malonaticus (n = 2), C. dublinensis (n = 2) and C. turicensis (n = 1). The MLST scheme produced 20 sequence types (STs), two of which were newly identified. ST148 was the most prevalent ST (n = 5), followed by ST4 (n = 3), ST17 (n = 3), ST64 (n = 3), and ST540 (n = 2). One (3.2%) and 15 (48.4%) Cronobacter isolates were resistant to tetracycline and meropenem, respectively. In contrast, all of the tested isolates were susceptible to the remaining 14 antibiotics. Moreover, 20 (64.5%) Cronobacter isolates showed weak ability to produce biofilm, but no isolates showed strong or moderate biofilm-forming ability. PRACTICAL APPLICATION: Our findings revealed a high genetic diversity of Cronobacter spp. in dried edible mushrooms and provided new epidemiological evidence for the widespread existence of Cronobacter spp. in such products. The presence of Cronobacter spp. in dried edible mushrooms may pose potential risks to human health and enhancing the hygiene of such products are necessary to ensure food safety.
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Agaricales , Cronobacter sakazakii , Cronobacter , Agaricales/genética , Antibacterianos/farmacología , Cronobacter/genética , Cronobacter sakazakii/genética , Farmacorresistencia Microbiana , Microbiología de Alimentos , Humanos , Lactante , Tipificación de Secuencias MultilocusRESUMEN
In this study, we investigated the structural characterization and antidiabetic effects of Lactobacillus H31 exopolysaccharide (EPS) H31-2 from pickled cabbage. The BLAST result indicated that Lactobacillus H31 was closely related to Lactobacillusplantarum S1S2L2. In the spectrum of Fourier-transform infrared spectroscopy (FT-IR), 3370â¯cm-1 was the characteristic band of polysaccharide. A structural analysis showed that the monosaccharides of EPS H31-2 included d-mannose (Man) and d-glucose (Glc). The molecular weight of EPS H31-2 was around 10.75â¯kDa. All these results suggested EPS H31-2 was a novel polysaccharide. The inhibition ratios of crude EPS H31 and the pure fraction H31-2 of pancreatic α-amylase were 89.1⯱â¯2.59% and 69.2⯱â¯8.95%, respectively. In addition, the supernatant glucose concentration of insulin-resistant HepG2 cells decreased by treatment with 10-8 M of EPS H31-2, which meant EPS H31-2 could promote the glucose uptake of insulin-resistant HepG2 cell. Furthermore, EPS H31-2 upregulated the expression of the GLUT-4, Akt-2, and AMPK, which play important roles in glycometabolism. These results suggested that Lactobacillus plantarum EPS H31-2 could effectively inhibit the activity of pancreas α-amylase and has potential applications in the prevention and alleviation of diabetes mellitus.
Asunto(s)
Biomarcadores de Tumor/metabolismo , Brassica/microbiología , Lactobacillus plantarum/química , Páncreas/enzimología , Polisacáridos Bacterianos/farmacología , Transducción de Señal , alfa-Amilasas/antagonistas & inhibidores , Proteínas Quinasas Activadas por AMP/metabolismo , Glucemia/análisis , Células Hep G2 , Humanos , Hipoglucemiantes/farmacología , Metilación , Monosacáridos/análisis , Fosfatidilinositol 3-Quinasas/metabolismo , Filogenia , Proteínas Proto-Oncogénicas c-akt/metabolismo , Espectroscopía de Protones por Resonancia Magnética , Transducción de Señal/efectos de los fármacos , Espectroscopía Infrarroja por Transformada de Fourier , alfa-Amilasas/metabolismoRESUMEN
Bacteriophages have key roles in regulating bacterial populations in most habitats. A Salmonella Typhimurium mutant (N18) with impaired sensitivity to phage fmb-p1 was obtained and examined, the adsorption efficiency of fmb-p1 to N18 was reduced to 6%, compared to more than 97% for wild type S. Typhimurium CMCC50115. Reduced adsorption was accompanied by a reduction of 90% in the LPS content compared to wild type. Electron microscopy showed phage scattered around N18 with minimal engagement, while the phage were efficiently adsorbed to the wild type with tails oriented towards the bacterial surface. Evidence suggests fmb-p1 can slightly infect N18 and this does not give rise to an increase of phage titer. RT-qPCR data show that several Salmonella genes involved in lipopolysaccharide synthesis and five virulence related genes were down-regulated upon exposure of N18 to phage fmb-p1. In contrast, phage resistance related genes such as the SOS response, restriction-modification (RM), and Cas1 gene were up-regulated in N18. These data suggest that although inefficient adsorption and entry is the primary mechanism of resistance, transcriptional responses to phage exposure indicate that alternative resistance mechanisms against phage infection are also brought to bear, including digestion of phage nucleic acids and activation of the SOS. These findings may help develop strategies for biocontrol of Salmonella where multi-resistant bacteria are encountered or emerge in applications for food production, bioremediation or wastewater treatment.
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Fagos de Salmonella/metabolismo , Salmonella typhimurium/genética , Salmonella typhimurium/virología , Acoplamiento Viral , Expresión Génica , Genoma Viral , Lipopolisacáridos/biosíntesis , Mutación , Respuesta SOS en Genética , Fagos de Salmonella/genética , Virulencia , Factores de Virulencia/genéticaRESUMEN
A core-shell structured chitosan (CS)-based gene vector with a sustainable gene transfection effect was designed and successfully prepared in this study. The pEGFP was first combined with the thiolated and N-alkylated chitosan (TACS). Then, hydroxybutyl chitosan grafted with poly(ethylene glycol) (EG-HBC) was coated on the pEGFP-loaded TACS particles. The prepared pEGFP-loaded TACS@EG-HBC particles have a size of about 200 nm and little cytotoxicity. The in vitro and in vivo gene transfection experiments indicate that the pEGFP-loaded TACS@EG-HBC particles possess a better sustainable gene transfection capacity and a high transfection efficiency, which should be attributed to the biodegradation of the CS-based shell, the thiolation and N-alkylation modification on CS cores, and the grafted PEG chains with better biocompatibility. The in vivo gene expression of the loaded pEGFP can persist up to 60 days. This novel gene vector has a theoretical and practical significance for gene therapy with sustained transfection effect.
RESUMEN
The combination of NIR photothermal therapy and chemotherapy is considered as the promising technique for future cancer therapy. The key point of this technique is the design and synthesis of photothermal agents with high-efficiency photothermal effects and high chemical drug loading capacity. Herein, submicron-sized raspberry-like hollow-structured polypyrrole microspheres (H-PPy) were facilely prepared through in situ polymerization of pyrrole on monodispersed polystyrene (PS) template microspheres with a diameter of 220 nm, followed by the chemical etching of PS templates. The prepared H-PPy microspheres show rapid and remarkable photothermal effects in water under NIR laser irradiation (808 nm) only for 5 min. Further, a model small molecular drug, (S)-(+)-camptothecin (CPT), was loaded into the void core by a simple dispersion-permeation process through the micro-pores on the raspberry-like PPy shell, with a loading capacity of 0.14 mg/(mg H-PPy). The MTT assay and the in vitro NIR-laser triggered release behavior indicated that pure H-PPy microspheres have good biosafety, but the release of loaded CPT into H-PPy microspheres can be achieved with remarkable spatial/temporal resolution after NIR laser irradiation, which results in excellent synergistic effect of photothermal and chemical ablation on HeLa cells, as proved by fluorescence microscopy. This work provides convenient synthesis of a promising cancer therapy agent with high drug-loading capacity and efficient NIR light photothermal effects, which can perfectly achieve the synergistic NIR photothermal therapy and chemotherapy of PPy microspheres.
RESUMEN
Novel submicron core-shell-structured chitosan-based composite particles encapsulated with enhanced green fluorescent protein plasmids (pEGFP) were prepared by complex coacervation method. The core was pEGFP-loaded thiolated N-alkylated chitosan (TACS) and the shell was pH- and temperature-responsive hydroxybutyl chitosan (HBC). pEGFP-loaded TACS-HBC composite particles were spherical, and had a mean diameter of approximately 120 nm, as measured by transmission electron microscopy and particle size analyzer. pEGFP showed sustained release in vitro for >15 days. Furthermore, in vitro transfection in human embryonic kidney 293T and human cervix epithelial cells, and in vivo transfection in mice skeletal muscle of loaded pEGFP, were investigated. Results showed that the expression of loaded pEGFP, both in vitro and in vivo, was slow but could be sustained over a long period. pEGFP expression in mice skeletal muscle was sustained for >60 days. This work indicates that these submicron core-shell-structured chitosan-based composite particles could potentially be used as a gene vector for in vivo controlled gene transfection.