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1.
Med Sci Monit ; 29: e940711, 2023 Jun 28.
Artículo en Inglés | MEDLINE | ID: mdl-37379270

RESUMEN

BACKGROUND The care ability of caregivers markedly impacts the patient' s quality of life and such ability may be influenced by various factors. This study aimed to explore the factors affected the care ability of caregivers for hemodialysis patients. MATERIAL AND METHODS This cross-sectional study involved 271 caregivers of hemodialysis patients. Various basic sociodemographic data of patients and caregivers were collected using questionnaires. The care abilities of caregivers were evaluated by the Caregiver Task Inventory (CTI). Univariate and multivariate linear regression analyses were used to identify the independent factors associated with the care ability of caregiver. The independent samples t test was used to further explore the impact of the independent factors on caregiver's care ability. RESULTS The mean age was 54.88±10.73 years for the patients and 44.68±15.22 years for the caregivers. Among the 271 hemodialysis patients, 59.04% were male. Multivariate regression analysis showed that female caregivers (standardized ß=-0.140, P=0.002), lived with patients (standardized ß=-0.381, P<0.001), high annual income of caregivers (standardized ß=-0.281, P<0.001), receiving caregiving training (standardized ß=-0.183, P<0.001), and patients without other chronic diseases (standardized ß=0.200, P<0.001) were associated with better care abilities of caregivers. CONCLUSIONS The gender and annual income of caregivers, receiving caregiving training, cohabitation with patient, and other concurrent chronic diseases of patients were the independent influencing factors for the care ability of caregivers for hemodialysis patients. Our study emphasized the necessity of implementing comprehensive socioeconomic and educational support to improve the care ability of caregivers.


Asunto(s)
Cuidadores , Calidad de Vida , Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Anciano , Estudios Transversales , Pacientes , Diálisis Renal
2.
Biomed Chromatogr ; 37(9): e5662, 2023 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-37185963

RESUMEN

Ellagic acid is one of the most representative natural antioxidants, and is rich in pomegranate peel. In this study, a consecutive countercurrent chromatographic (CCC) separation method was established to improve the preparative efficiency of ellagic acid from pomegranate peel. By optimizing the solvent system, sample size and flow rate, 280 mg of ellagic acid was obtained from 5 g of crude sample from pomegranate peel by CCC after six consecutive injections. Moreover, the values of EC50 for ellagic acid in scavenging ABTS·+ and DPPH· were 4.59 ± 0.07 and 10.54 ± 0.07 µg/ml, respectively, indicating a strong antioxidant activity. This study not only established a high-throughput method for the preparation of ellagic acid, but also provided a successful example for the development of and research on other natural antioxidants.


Asunto(s)
Lythraceae , Granada (Fruta) , Antioxidantes/análisis , Ácido Elágico/análisis , Ácido Elágico/química , Lythraceae/química , Extractos Vegetales/química
3.
Nano Lett ; 22(14): 5803-5809, 2022 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-35848711

RESUMEN

An organic light-emitting transistor (OLET) is a candidate device architecture for developing electrically pumped organic solid-state lasers, but it remains a critical challenge because of the lack of organic semiconductors that simultaneously possess a high solid-state emission efficiency (Φs), a high and balanced ambipolar mobility (µh,e), and a large stimulated emission cross-section. Here, we designed a molecule of 4,4'-bis(2-dibenzothiophenyl-vinyl)-biphenyl (DBTVB) and prepared its ultrathin single-crystal microplates with herringbone packing arrangements, which achieve balanced mobilities of µh = 3.55 ± 0.5 and µe = 2.37 ± 0.5 cm2 V-1 s-1, a high Φs of 85 ± 3%, and striking low-threshold laser characteristics. Theoretical and experimental investigations reveal that a strong electronic coupling and a small reorganization energy ensure efficient charge transport; meanwhile, the exciton-vibration effect and negligible π-π orbital overlap give rise to highly emissive H-aggregates and facilitate laser emission. Furthermore, OLET-based DBTVB crystals offer an internal quantum efficiency approaching 100% and a record-high electroluminescence external quantum efficiency of 4.03%.

4.
Angew Chem Int Ed Engl ; 62(20): e202302629, 2023 May 08.
Artículo en Inglés | MEDLINE | ID: mdl-36929615

RESUMEN

The dual-ratiometric thermometry is one of highly accurate methods for microscopic thermal measurement in biological systems. Herein, a series of chromone derivatives with noncovalently intramolecular interactions (NIIs) were designed and synthesized for ratiometric thermometers. The triplet states of these organic compounds were systematically tuned upon regulating the conformation with NIIs to yield efficient room temperature phosphorescence and large wavelength difference between fluorescence and phosphorescence simultaneously. As a result, an unprecedent organic 3D dual-ratiometric thermometer was established based on the intensity ratio and lifetime ratio of fluorescence/phosphorescence vs temperature, which was used for in vitro and in vivo bio-thermometry with high accuracy. This work provides a novel method to achieve organic dual ratiometric thermometers via tuning the triplet excited states.

5.
J Am Chem Soc ; 144(28): 12652-12660, 2022 07 20.
Artículo en Inglés | MEDLINE | ID: mdl-35762534

RESUMEN

Stimuli-responsive functional luminescent materials with tunable color and long-persistent emission have emerged as a powerful tool in information encryption, anticounterfeiting, and bioelectronics. Herein, we prove a novel strategy for manipulating the proton transfer pathways in the salicylaldehyde derivative EQCN solutions/powder to produce excitation wavelength-dependent (Ex-De) performances with switchable emissions (blue-sky, green, and orange). The experiments and theoretical results demonstrated that the different luminous colors are originated from enol (E) form (blue-sky), Keto-1 (K1) form (orange) through the excited-state intramolecular proton transfer (ESIPT) process, and Keto-2 (K2) form (green) through the excited-state long-range proton transfer (ESLRPT) process. We leverage synergistic effects between the dopant and matrix (dimethyl terephthalate, DTT) to manipulate the excited-state proton transfer pathway in EQCN@DTT mixture powders to generate Ex-De long-persistent luminescence (Ex-De-LPL), which can be well applied in multilevel information encryption. This strategy not only paves an intriguing way for the construction and preparation of pure organic Ex-De materials but also offers a guideline for developing LPL materials based on ESLRPT processes.


Asunto(s)
Luminiscencia , Protones , Alcoholes
6.
Nano Lett ; 21(8): 3487-3494, 2021 04 28.
Artículo en Inglés | MEDLINE | ID: mdl-33848175

RESUMEN

Stimulated emission depletion (STED) nanoscopy plays a key role in achieving sub-50 nm high spatial resolution for subcellular live-cell imaging. To avoid re-excitation, the STED wavelength has to be tuned at the red tail of the emission spectrum of fluorescent probes, leading to high depletion laser power that might damage the cell viability and functionality. Herein, with the highly emissive silica-coated core-shell organic nanoparticles (CSONPs) enabling a giant Stokes shift of 150 nm, ultralow power STED is achieved by shifting the STED wavelength to the emission maximum at 660 nm. The stimulated emission cross section is increased by ∼20-fold compared to that at the emission red tail. The measured saturation intensity and lateral resolution of our CSONP are 0.0085 MW cm-2 and 25 nm, respectively. More importantly, long-term (>3 min) dynamic super-resolution imaging of the lysosomal fusion-fission processes in living cells is performed with a resolution of 37 nm.


Asunto(s)
Nanopartículas , Colorantes Fluorescentes , Rayos Láser , Microscopía Fluorescente , Dióxido de Silicio
7.
Angew Chem Int Ed Engl ; 61(29): e202201588, 2022 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-35536106

RESUMEN

Multi-resonance TADF (MR-TADF) emitters are promising for high-resolution OLEDs, but the concurrent optimization of excited-state dynamics and color purity remains a tough challenge. Herein, three deep-blue MR-TADF compounds (BN1-BN3) featuring gradually enlarged ring-fused structures and increased rigidity are accessed by lithium-free borylation in high yields from the same precursor, with all the emitters possessing CIEy coordinates below 0.08. Structure-property investigations demonstrate a strategic improvement of the oscillator strength (fosc ) and acceleration of the reverse intersystem crossing (RISC) process by extending the π-skeleton, where BN3 realizes a maximum external quantum efficiency (EQE) of 37.6 % and reduced roll-off, thus showing the best efficiency reported for deep-blue TADF OLEDs. The internal regulation of the efficiency and color purity of these compounds validate the general effectiveness to achieve advanced deep-blue narrowband emitters with higher-order boron/nitrogen-based MR motifs.

8.
Angew Chem Int Ed Engl ; 61(38): e202206916, 2022 Sep 19.
Artículo en Inglés | MEDLINE | ID: mdl-35754001

RESUMEN

Polycyclo-heteraborin multi-resonance (MR) emitters are promising for high color-purity organic light-emitting diodes (OLEDs). Here, unlike the most common heteroatom ternary-doped (X/B/N) frameworks, a binary-doped (B/N) skeleton is reported with a large energy band for wide-range color tunability. Based on this parent-segment, a "one-pot" catalyst-free borylation method is developed which generates deep blue to pure green MR emitters from readily available starting materials, with peaks at 426-532 nm and full-width-at-half-maxima of 27-38 nm. Impressively, a maximum external quantum efficiency of nearly 40 % is recorded for the corresponding device with Commission Internationale de l'Eclairage coordinates of (0.14, 0.16), representing the state-of-the-art performances. This work presents a new paradigm and synthesis of B/N-doped MR emitters and will motivate the study of other novel frameworks.

9.
Opt Express ; 29(9): 12833-12848, 2021 Apr 26.
Artículo en Inglés | MEDLINE | ID: mdl-33985031

RESUMEN

We have proposed the profile-based intensity and frequency corrections for single-snapshot spatial frequency domain (SFD) imaging to mitigate surface profile effects on the measured intensity and spatial frequency in extracting the optical properties. In the scheme, the spatially modulated frequency of the projected sinusoidal pattern is adaptively adjusted according to the sample surface profile, reducing distortions of the modulation amplitude in the single-snapshot demodulation and errors in the optical property extraction. The profile effects on both the measured intensities of light incident onto and reflected from the sample are then compensated using Minnaert's correction to obtain the true diffuse reflectance of the sample. We have validated the method by phantom experiments using a highly sensitive SFD imaging system based on the single-pixel photon-counting detection and assessed error reductions in extracting the absorption and reduced scattering coefficients by an average of 40% and 10%, respectively. Further, an in vivo topography experiment of the opisthenar vessels has demonstrated its clinical feasibility.

10.
BMC Genomics ; 20(1): 766, 2019 Oct 22.
Artículo en Inglés | MEDLINE | ID: mdl-31640547

RESUMEN

BACKGROUND: Rhizome is the storage underground stem of lotus (Nelumbo nucifera), which is enlarged before winter season and could be used for asexual propagation. In addition, the enlarged rhizome is a nutritional vegetable with abundant starch, proteins, and vitamins. Enlargement of lotus rhizome is not only significance for itself to survive from the cold winter, but also important for its economic value. RESULTS: To explore the mechanism underlying its enlargement, integrative analyses of morphology, physiology and proteomics were conducted on the rhizome at stolon, middle, and enlarged stages. Morphological observation and physiological analyses showed that rhizomes were gradually enlarged during this process, in which the starch accumulation was also initiated. Quantitative proteomic analysis on the rhizomes at these three stages identified 302 stage-specific proteins (SSPs) and 172 differently expressed proteins (DEPs), based on which GO and KEGG enrichment analyses were conducted. The results indicated that light and auxin signal might be transduced through secondary messenger Ca2+, and play important roles in lotus rhizome enlargement. CONCLUSION: These results will provide new insights into understanding the mechanism of lotus rhizome enlargement. Meanwhile, some candidate genes might be useful for further studies on this process, as well as breeding of rhizome lotus.


Asunto(s)
Nelumbo , Rizoma/crecimiento & desarrollo , Rizoma/metabolismo , Transducción de Señal , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Modelos Biológicos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteómica , Rizoma/genética , Almidón/metabolismo
11.
Analyst ; 144(17): 5136-5142, 2019 Aug 16.
Artículo en Inglés | MEDLINE | ID: mdl-31338492

RESUMEN

γ-Glutamyl transpeptidase (GGT) has been reported as a biomarker of hepatocellular carcinoma (HCC), and its imaging is of great benefit for early detection in precise medicine as well as intraoperative navigation. Herein, we have designed and synthesized a novel near-infrared fluorescent probe coupled aggregation-induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) effect for the detection of GGT. Thanks to conjugated glutamate acid, this probe could be dispersed in aqueous solution and showed barely any fluorescence emission. Through a GGT-mediated enzymatic reaction, the aggregation state of the probe in aqueous solution was changed and an intramolecular hydrogen bond was formed, resulting in an enhanced fluorescence emission. An excellent linear relationship was observed and the concentration of GGT measured was in the range of 10-90 U L-1 with a limit of detection calculated at 2.9 U L-1. Its feasibility has been confirmed by detecting GGT in HepG2 cells with high specificity and long-term sustainability, satisfying clinical need. Moreover, this nanoprobe showed great potential for precise medicine guided surgery by realizing fluorescence imaging in human liver tumour tissue and distinguishing it from normal tissue. Thus, we supposed that our AIE coupled ESIPT fluorescent nanoprobe has great potential in the early detection of HCC, the selective fluorescence imaging of GGT positive cells during surgery and application in precision medicine.


Asunto(s)
Biomarcadores de Tumor/análisis , Colorantes Fluorescentes/química , gamma-Glutamiltransferasa/análisis , Benzotiazoles/síntesis química , Benzotiazoles/química , Colorantes Fluorescentes/síntesis química , Glutamatos/síntesis química , Glutamatos/química , Células Hep G2 , Humanos , Límite de Detección , Neoplasias Hepáticas/diagnóstico , Microscopía Confocal/métodos , Microscopía Fluorescente/métodos , Medicina de Precisión/métodos , Sensibilidad y Especificidad
12.
Mikrochim Acta ; 186(6): 353, 2019 05 16.
Artículo en Inglés | MEDLINE | ID: mdl-31098674

RESUMEN

The authors describe the syntheses and application of glutathione-capped gold nanoclusters (AuNCs) with thermoresponsive properties. The AuNCs have excitation/emission maxima at 430/610 nm and the bright redfluorescence changes along with the temperature in the range from 0 to 90 °C which covers the normal temperature range of living cells. In the range of physiological temperatures (35-42 °C), the temperature resolution is 0.73 °C. The AuNCs display excellent colloidal stability and biocompatibility. They were used for fluorometric temperature detection and imaging of hepatic stellate cells. With such attractive features, the AuNCs are quite promising luminescence nanothermometers. Graphical abstract Schematic presentation of the fluorescence of glutathione-capped gold nanoclusters (AuNCs) as nanothermometers in living cells. The AuNCs have excitation/emission maxima at 430/610 nm and the red fluorescence changes with temperature in a wide range of 0 to 90 °C which covers the normal temperature of living cells.


Asunto(s)
Fluorometría , Oro/química , Nanopartículas del Metal/química , Nanotecnología , Imagen Óptica , Temperatura , Supervivencia Celular , Células Cultivadas , Fluorometría/instrumentación , Humanos , Rayos Infrarrojos , Nanotecnología/instrumentación
13.
Int J Toxicol ; 35(6): 712-718, 2016 11.
Artículo en Inglés | MEDLINE | ID: mdl-27694306

RESUMEN

With the increasing use of mycophenolic acid (MPA) in solid organ transplantation, some clinical studies indicate that it is also a human teratogen. However, it is unknown by which mechanism MPA acts as a teratogen. Mycophenolic acid was a selective blocker of de novo purine synthesis, and its immunosuppressive effect is mediated by the inhibition of inosine monophosphate dehydrogenase, which could be a target for MPA-induced toxicity as well. The aim of our study was to examine the direct influence of MPA exposure on zebrafish (Danio rerio) embryos. Morphological defects including tail curvature and severe pericardial edema in zebrafish embryos caused by MPA (3.7-11.1 µmol/L) were found in a dose-dependent manner. The teratogenic index (25% lethal concentration value (LC25)/no observed adverse effect level ratio) was 16, which indicated MPA as a teratogen. Quantitative polymerase chain reaction analysis revealed that the expression level of impdh1b and impdh2 was significantly reduced by MPA treatment at 8 µmol/L (equals to LC25 level). All the toxic effects could be partially reversed by the addition of 33.3 µmol/L guanosine. Our results indicated that MPA impairs the development of zebrafish embryos via inhibition of impdh activity, which subsequently caused a guanosine nucleotide depletion in vivo.


Asunto(s)
IMP Deshidrogenasa/antagonistas & inhibidores , Inmunosupresores/toxicidad , Ácido Micofenólico/toxicidad , Teratógenos/toxicidad , Animales , Embrión no Mamífero , Desarrollo Embrionario/efectos de los fármacos , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Guanosina/farmacología , IMP Deshidrogenasa/genética , Dosificación Letal Mediana , Nivel sin Efectos Adversos Observados , Cola (estructura animal)/anomalías , Pez Cebra
14.
Apoptosis ; 20(3): 410-20, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25596717

RESUMEN

Imaging agents that enable direct detection of apoptosis are highly desirable in the field of monitoring chemotherapeutic response as well as early diagnosis and disease monitoring. Previous work demonstrated that the dansyled amino acid DNSBA is used to specifically and selectively detect apoptotic cancer cells at the both early and late stages, but the mechanism remains unclear. In this work, we evaluated DNSBA as a tool for monitoring cell apoptosis in CNE1 tumor cell models both in vitro and ex vivo after its in vivo administration, which was confirmed by other assays. The ability of DNSBA to detect multiple pathways and different stages of apoptosis leading to cell death may be advantageous in the evaluation of cancer treatment indicative of a positive therapeutic outcome. The uptake change of molecular probes DNSBA in CNE1 cells represented the changes of apoptotic rate in a caspase-dependent manner. However, the accumulation of DNSBA in apoptotic cells did not increase with the enhanced membrane permeability. Furthermore, ex vivo study demonstrated DNSBA has a similar pattern as the TUNEL-positive cells. In conclusion, DNSBA cellular imaging is useful for the early assessment of treatment-induced apoptosis, and thus may act as a substitute for Annexin V for assessing treatment response.


Asunto(s)
Compuestos de Dansilo/metabolismo , Sondas Moleculares/metabolismo , Neoplasias Nasofaríngeas/genética , Imagen Óptica/métodos , Fenilpropionatos/metabolismo , Animales , Anexina A5/genética , Anexina A5/metabolismo , Apoptosis/efectos de los fármacos , Transporte Biológico , Carcinoma , Caspasas/genética , Caspasas/metabolismo , Línea Celular Tumoral , Permeabilidad de la Membrana Celular , Compuestos de Dansilo/química , Expresión Génica , Humanos , Etiquetado Corte-Fin in Situ , Inyecciones Subcutáneas , Cinética , Ratones , Ratones Endogámicos BALB C , Sondas Moleculares/química , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/diagnóstico , Neoplasias Nasofaríngeas/metabolismo , Neoplasias Nasofaríngeas/patología , Trasplante de Neoplasias , Paclitaxel/farmacología , Fenilpropionatos/química , Factor de Necrosis Tumoral alfa/farmacología
15.
Analyst ; 140(6): 1814-6, 2015 Mar 21.
Artículo en Inglés | MEDLINE | ID: mdl-25652214

RESUMEN

A new ESIPT dye of benzothiazole with a conjugative electron acceptor was discovered and synthesized. Due to its unique NIR keto emission and a large Stokes shift, it was used to develop a fluorescent probe for sensitive and selective detection of hydrogen sulfide.


Asunto(s)
Benzotiazoles/química , Colorantes Fluorescentes/química , Sulfuro de Hidrógeno/análisis , Espectrometría de Fluorescencia
16.
Water Res ; 264: 122215, 2024 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-39154536

RESUMEN

The excessive presence of geogenic ammonium (NH4+) in groundwater poses a global environmental concern, commonly linked to the degradation of nitrogen-containing dissolved organic matter (DOM). However, there is a gap in systematic studies on the combination of soluble organic matter (SOM) in sediments and DOM in groundwater, with few indoor incubation experiments to validate their degradation pathways. This study utilized ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry to analyze the molecular characteristics of DOM and SOM in aquifer systems affected by geogenic NH4+. Subsequently, indoor incubation experiments spanning up to 140 d were conducted to verify the degradation pathways. The experimental results revealed a two-phase degradation process for both the DOM and SOM. The initial stage was characterized by the degradation of aliphatic compounds (ALC) with the production of polyphenols (PPE) and highly unsaturated compounds (HUC). The second stage was dominated by the degradation of PPE and HUC, accompanied by the re-consumption of some ALC, while more recalcitrant HUC persisted. Notably, the first stage of SOM degradation exceeded that of DOM degradation, indicating that SOM exhibited greater resistance to aging. This phenomenon may be attributed to a wider range of active enzymes in sediments, the rapid replenishment of SOM by organic matter in sediments, or the accelerated degradation of DOM. The experimental results aligned with the molecular characterization of DOM and SOM in actual aquifer systems. It is hypothesized that NH4+ produced through the direct mineralization of SOM may contribute more to the enrichment of NH4+ in groundwater than that produced through the mineralization of DOM. This study is the first to analyze DOM and SOM together in aquifer systems and validate their degradation pathways through incubation experiments, thereby providing novel insights into the enrichment of geogenic NH4+ in groundwater.


Asunto(s)
Compuestos de Amonio , Agua Subterránea , Contaminantes Químicos del Agua , Agua Subterránea/química , Contaminantes Químicos del Agua/química , Sedimentos Geológicos/química , Compuestos Orgánicos/química , Solubilidad
17.
ACS Omega ; 9(24): 26058-26065, 2024 Jun 18.
Artículo en Inglés | MEDLINE | ID: mdl-38911722

RESUMEN

Rabies is a zoonotic infection with the potential to infect all mammals and poses a significant threat to mortality. Although enzyme-linked immunosorbent tests and real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) have been established for rabies virus (RABV) detection, they require skilled staff. Here, we introduce a personal glucose meter (PGM)-based nucleic acid (NA-PGM) detection method to diagnose RABV. This method ensures sensitive and convenient RABV diagnosis through hybridization of reverse transcription-recombinase aided amplification (RT-RAA) amplicons with probes labeled with sucrose-converting enzymes, reaching a detection level as low as 6.3 copies/µL equivalent to 12.26 copies. NA-PGM allows for the differentiation of RABV from other closely related viruses. In addition, NA-PGM showed excellent performance on 65 clinical samples with a 100% accuracy rate compared with the widely adopted RT-qPCR method. Thus, our developed NA-PGM method stands out as sensitive, semiquantitative, and portable for RABV detection, showcasing promise as a versatile platform for a wide range of pathogens.

18.
Food Chem ; 447: 138954, 2024 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-38461716

RESUMEN

Real-time optical sensing of mercury has been developed rapidly in recent years but remains challenging such as bearing background interference. Herein, a Hg2+ and base dual-activatable ultrasensitive chemiluminescent probe CL-Hg based on benzothiazole-phenoxyl-dioxetane with profits of excitation light-free and minimal interference is presented. The photophysical properties study and sensing performance verified CL-Hg is coupled with unique advantages of long-term detection (more than 400 min), ultrahigh sensitivity (LOD = 0.52 nM), and high specificity to Hg2+, and visualization detection by the paper-based test strips. More importantly, CL-Hg showed the qualitative and quantitative detection capability for Hg2+ with great recyclability in real samples of water, seafood, and beverages, holding great potential for on-site monitoring of Hg2+ levels in the actual samples. To our knowledge, this is the first work achieving the detection of Hg2+ by chemiluminescence. Overall, the Hg2+-activated visualization platform offers a practical method for detecting Hg2+ in various application scenarios.


Asunto(s)
Mercurio , Mercurio/análisis , Agua , Bebidas , Colorantes Fluorescentes
19.
TechTrends ; 67(2): 342-353, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36466739

RESUMEN

This yearlong study describes multiple stakeholders' perspectives of 20 preservice English as Foreign Language (EFL) teachers, 43 elementary school students, 2 online mentors, and a teacher-researcher during a technology professional development practicum on a cloud. The case study provides qualitative and quantitative data from stakeholders concerning technology integration after participating in online project-based EFL instruction. The participating stakeholders encountered affordances and challenges that enhanced their online learning and teaching repertoires and offered nuanced evidence within this online professional development community. The findings call for continuing exploration of online practicums in preservice (language) teacher education and further research documenting complexities of multiple stakeholders' technology professional development.

20.
J Proteomics ; 270: 104736, 2023 01 06.
Artículo en Inglés | MEDLINE | ID: mdl-36174953

RESUMEN

Seed longevity is important for the maintenance of seed nutritional quality, vigor, and germination potential during storage. Sacred lotus is known as one of the longest living seeds in the world and their ability to maintain longevity has been widely investigated. In this study, a suitable controlled deterioration treatment (CDT) method was first established to evaluate the vigor loss of lotus plumule (LP), and then the Tandem Mass Tags (TMT)-based proteomic analysis was performed on LP from the CDT-treated seed to quantitatively and qualitatively analyze the protein profile dynamic. In total, 4002 proteins were successfully quantified, of them, 558 differently accumulated proteins (DAPs) were identified. Protein processing and RNA-related proteins were found more easily to be affected by CDT, which may directly result in seed vigor loss. Meanwhile, CDT resulted in remarkable up-regulation of numerous proteins related to antioxidation, photosynthesis, RNA and DNA stability, starch and sucrose mobilization, and cell membrane and wall stability, which potentially played key roles in maintaining the lotus seed vigor under CDT. Histological and physiological analyses were also performed to verify some proteome results. This study provided both fundamental data and new insights to further uncover the secret of lotus seed longevity. SIGNIFICANCE: Seed aging affects the seed quality and can result in direct economic losses. The exceptional longevity of sacred lotus seed has attracted extensive attention. In this study, an optimized CDT method was used to mimic the natural aging process of sacred lotus seed, and based on TMT-based quantitative proteomic analysis on the LP profile of CDT-treated seeds, a series of differentially accumulation of specific proteins (DEPs) were revealed related to CDT resistance. Correspondingly, the physiological state and histological structure of the LP along with the CDT were detected to verify the proteome data. This study provided comprehensive information for the molecular basis of lotus seed aging analysis and facilitate to screen seed longevity related proteins for other plant species.


Asunto(s)
Nelumbo , Nelumbo/genética , Nelumbo/metabolismo , Proteómica/métodos , Proteoma/metabolismo , Proteínas de Plantas/metabolismo , Semillas/metabolismo , ARN
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