RESUMEN
Insertion of foreign oligopeptide sequences (40-50 amino acids in length) into the Pro144 position of hepatitis B core antigen (HBcAg) leads to the formation of chimeric capsids in Escherichia coli cells. These capsids are morphologically and immunologically similar to native HBcAg, but expose the inserted oligopeptides on their outer surface and exhibit antigenic and immunogenic characteristics of the latter. As a source of model antigenic determinants, the appropriate DNA copies excised from cloned viral genes such as the pre-S region of hepatitis B virus, the transmembrane protein gp41 of human immunodeficiency virus 1 and the envelope protein gp51 of bovine leukemia virus have been used. The localization of the inserted antigenic determinants on the surface of chimeric capsids does not depend on the presence or absence of the arginine-rich, 39 amino acid-long C terminus of HBcAg.
Asunto(s)
Antígenos Virales/genética , Antígenos de la Hepatitis B/genética , Antígenos del Núcleo de la Hepatitis B/inmunología , Cápside/inmunología , ADN Recombinante , Epítopos , Genes Virales , Vectores Genéticos , Proteína gp41 de Envoltorio del VIH/genética , Proteína gp41 de Envoltorio del VIH/inmunología , Antígenos del Núcleo de la Hepatitis B/genética , Virus de la Hepatitis B/genética , Virus de la Hepatitis B/inmunología , Virus de la Hepatitis B/ultraestructura , Inmunohistoquímica , Virus de la Leucemia Bovina/genética , Virus de la Leucemia Bovina/inmunología , Datos de Secuencia Molecular , Vacunas Sintéticas , Proteínas del Envoltorio Viral/genética , Proteínas del Envoltorio Viral/inmunologíaRESUMEN
The possibility of using recombinant HBcAg, synthesized in Escherichia coli, for diagnostic purposes has been studied. The antigen can be used in highly sensitive immunological assays for the detection of IgG and IgM antibodies to HBcAg.
Asunto(s)
Ingeniería Genética , Antígenos del Núcleo de la Hepatitis B/análisis , Escherichia coli/inmunología , RadioinmunoensayoRESUMEN
The coating of titanium nitride increases the working life a microsurgical instruments by 1.5 times. The coating is resistant to the bactericidal treatment.
Asunto(s)
Compuestos de Aluminio , Aluminio/análisis , Microcirugia/instrumentación , Oftalmología/instrumentación , Instrumentos Quirúrgicos/normas , Titanio/análisis , Carbono/análisis , Diseño de Equipo , Pruebas de Dureza , Propiedades de Superficie , Resistencia a la Tracción , U.R.S.S.Asunto(s)
Donantes de Sangre , Portador Sano , Anticuerpos contra la Hepatitis B/análisis , Antígenos del Núcleo de la Hepatitis B/inmunología , Hepatitis B/prevención & control , Reacción a la Transfusión , Ensayo de Inmunoadsorción Enzimática , Hepatitis B/etiología , Hepatitis B/inmunología , HumanosRESUMEN
The article presents a set of newly elaborated specific techniques of the indication of protective pastes and ointments penetrability into the sealants. The techniques are based on the sealant's colour reactions to iron chloride spirits solution. It was established that skin protecting silicone creams, EIR-1 and HIOT pastes were sealant resistant for 4-6 minutes only. Medicinal vaseline displayed its protection properties for 6 hours, thus demonstrating its greater effectiveness when used in industrial conditions.