[Management of a patient with diabetes mellitus after total pancreatectomy. Case report].

In modern diabetology, the most important condition for a personalized approach to patient management is to determine the type of diabetes mellitus. Particular attention is drawn to a large, but at the same time insufficiently studied group of patients with diabetes mellitus due to diseases of the pancreas or as a result of surgical interventions on the pancreas, in particular, patients who, for a number of vital indications, undergo total pancreatectomy and the mechanism of development of impaired glucose homeostasis have not been studied in these patients. To date, there are no specific algorithms for managing this category of patients. This clinical example emphasizes that the management of glycemia in patients with diabetes mellitus as a result of total pancreatectomy is an extremely difficult task that requires a multidisciplinary approach in the treatment of this category of patients, the participation of an endocrinologist at all stages of patient treatment.

DISEASES OF THE ORAL MUCOSA IN PATIENTS IN THE POST-COVID PERIOD.

The impact of SARS-CoV-2 (severe acute respiratory syndrome-related coronavirus-2) on the entire human body causes irreversible changes in all organs and systems. Complications in the form of chronic diseases require the treatment of clinicians in various fields of medicine. Dentists are faced with diseases of the oral mucosa in apparently healthy patients who are not predisposed to them but have undergone COVID-19. Purpose of the study - to assess the impact of the coronavirus infection (COVID-19) on the course of diseases of the oral mucosa. We examined 51 patients aged 20 to 75 who had undergone COVID-19 with various diseases of the oral mucosa. The study used clinical and laboratory methods. The features of the clinical course of certain diseases of the oral mucosa were revealed, the dynamics of the local immune defense of the oral cavity in the post-covid period was determined.

Analyzing Nonequilibrium Quantum States through Snapshots with Artificial Neural Networks.

Current quantum simulation experiments are starting to explore nonequilibrium many-body dynamics in previously inaccessible regimes in terms of system sizes and timescales. Therefore, the question emerges as to which observables are best suited to study the dynamics in such quantum many-body systems. Using machine learning techniques, we investigate the dynamics and, in particular, the thermalization behavior of an interacting quantum system that undergoes a nonequilibrium phase transition from an ergodic to a many-body localized phase. We employ supervised and unsupervised training methods to distinguish nonequilibrium from equilibrium data, using the network performance as a probe for the thermalization behavior of the system. We test our methods with experimental snapshots of ultracold atoms taken with a quantum gas microscope. Our results provide a path to analyze highly entangled large-scale quantum states for system sizes where numerical calculations of conventional observables become challenging.

[Analysis of dental care provided to the adult population with infectious diseases in in-patient departments]. / Analiz vidov i ob"emov stomatologicheskoi pomoshchi, okazyvaemoi vzroslomu naseleniyu s infektsionnymi boleznyami v usloviyakh statsionara.

The purpose of the study was to analyze the types and volumes of treatment and prophylactic services provided to the adult population with infectious diseases in the dental office of a multidisciplinary hospital. MATERIALS AND METHODS: The types and volumes of dental care provided to the adult population with infectious diseases in a multidisciplinary hospital have been analyzed. RESULTS: The study showed that dentists use 49 types of services when treating adults with infectious diseases, which corresponds to 29.88% of the compulsory medical insurance classifier in Moscow. Provided services can be conditionally divided into 4 groups: consultation, diagnostic, preventive and medicinal manipulations. The share of consulting services corresponds to 18.16±0.41% of the total volume of services provided; on average patients made 320.8 visits to the dentist's doctors per year, which corresponds to 2.38 visits for each patient. The proportion of medicinal manipulations, diagnostic and preventive services corresponds to 68.64±0.49%, 7.02±0.27% and 6.17±0.26%, respectively. Services related to the diagnosis and treatment of diseases of the oral mucosa and salivary glands dominate with 69.3%, on average 5.51 services are provided per visit. CONCLUSION: The compulsory medical insurance program for the city of Moscow should provide services for the diagnosis, treatment and prevention of dental diseases, provided by doctors specializing in «dentistry¼, for patients with infectious diseases who are undergoing treatment in inpatient conditions.

Design of the shattered pellet injection system for ASDEX Upgrade.

A new shattered pellet injection system was designed and built to perform disruption mitigation experiments on ASDEX Upgrade. The system can inject pellets with diameters of 1, 2, 4, or 8 mm with variable lengths over a range of L/D ratios of ∼0.5-1.5. By using helium or deuterium as propellant gas, the pellets can be accelerated to speeds between 60 and 750 m/s. The velocity range slightly depends on the pellet mass. The injection system is capable of preparing three pellets in separate barrels at the same time. Once accelerated by the propellant gas pulse, the pellets travel through one of three parallel flight tubes. Each flight tube is separated into three sections with increasing diameters of 12, 14, and 16 mm. Two gaps between the sections allow for removal of the propellant gas by expansion into two separate expansions tanks (0.3 and 0.035 m3), pellet observation in the first gap and the torus gate valve in the second. Each flight tube end is equipped with an exchangeable shatter head with different shatter angles, square or circular cross-section, and different lengths. The gas preparation and control systems allow highly automated pellet generation for precision of the pellet composition and an excellent reproducibility of shattered pellet experiments.

Regimes of ion dynamics in current sheets: The machine learning approach.

Current sheets are spatially localized almost-one-dimensional (1D) structures with intense plasma currents. They play a key role in storing the magnetic field energy and they separate different plasma populations in planetary magnetospheres, the solar wind, and the solar corona. Current sheets are primary regions for the magnetic field line reconnection responsible for plasma heating and charged particle acceleration. One of the most interesting and widely observed types of 1D current sheets is the rotational discontinuity, which can be force-free or include plasma compression. Theoretical models of such 1D current sheets are based on the assumption of adiabatic motion of ions, i.e., ion adiabatic invariants are conserved. We focus on three current sheet configurations, widely observed in the Earth magnetopause and magnetotail and in the near-Earth solar wind. The magnetic field in such current sheets is supported by currents carried by transient ions, which exist only when there is a sufficient number of invariants. In this paper, we apply a machine learning approach, AI Poincaré, to determine parametrical domains where adiabatic invariants are conserved. For all three current sheet configurations, these domains are quite narrow and do not cover the entire parametrical range of observed current sheets. We discuss possible interpretation of obtained results indicating that 1D current sheets are dynamical rather than static plasma equilibria.

Assessment of fish health status in the Pechora River: effects of contamination.

The present study aimed to assess the ecological situation in the Pechora River Basin (east part of Sub-Arctic Russia) using histopathologies of fish and to relate fish health to environmental quality. This paper reports histopathological alterations of fish kidney, liver, and gills and their association with chemical contamination of the Pechora River. A variety of histopathological changes was found. Differences between studied species and sites of the Pechora River with regard to the type, prevalence, and severity of lesions were studied. The types of the lesions indicated that fish respond to both direct toxicant effects of contaminated water and sediment, and secondary stress effects caused by factors such as parasitism. The structural modifications found in this study are a result of acute damage associated with short-term exposure as much as chronic response due to long-term pollution.

[Food behavior of consumers (for example, Chelyabinsk)].

Sociological poll of inhabitants of Chelyabinsk concerning a balanced diet was conducted. Every second chelyabinets needs change of the food, but thus has for this purpose no sufficient money resources. Deficiency in a food of animal protein, the majority of vitamins, macro- and microelements and saturation of a diet by carbohydrates and fats was shown. It was established that the products of mass consumption: bakery, dairy, meat,fat-and-oil products and sweets can be considered as potential products for enrichment, oriented for low-and middle-income countries primarily.

The Oral Mucosa Status and the Correlation between the Functional Parameters and the Level of Nitric Oxide Metabolites in Saliva among Patients with GERD.

The study involved 91 patients (48 women and 43 men), aged from 18 to 70 years with GERD. All patients underwent the clinical dental examination according to a single scheme including general clinical manifestations (nausea, single vomiting, belching, heartburn, pain in the epigastrium and around the navel, and poor appetite) and dental manifestations of GERD. The objective assessment of the dental status of the examined patients included the measurement of the functional parameters of the mixed saliva, buffer capacity (BC) of saliva, and the detection of the nitric oxide metabolites (NOx) content in saliva from the right parotid salivary gland ("SRPSG") and in blood serum using the indirect method based on the determination of the stable metabolites: nitrates and nitrites using the Griess reaction. It was established that salivation rate among patients with GERD with the prevailing of ACR and SACR was at the lower limit of normal values (0.32 + 0.19 ml/min), and the salivation rate among patients with the prevailing of SALCR was low (0.10 + 0.04 ml/min). The BC of saliva among patients with the prevailing of ACR and SACR was high (9.07 + 1.23 mmol eq/l and 9.40 + 1.71 mmol eq/l, respectively) and was reduced among patients with the prevailing of SALCR (7.63 + 0.18 mmol eq/l). The NOx level in SRPSG among patients with GERD was increased (especially in Group 3 (20.93 + 11.23 umol/l)). The direct correlation between the indicators of sialometry, the level of the BC of saliva, and the NOx level in SRPSG were established during the study.

[Synthesis of spyropyran derivatives of retinal and study of their interaction with bacterioopsin from Halobacterium salinarum].

The synthesis of retinal analogue series that contain a spyropyran moiety instead of a trimethylcyclohexene ring was proposed. The process of the retinal analogue interaction with bacterioopsin from apomembranes of Halobacterium salinarum and the spectral properties of the newly formed pigments were studied. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2008, vol. 34, no. 2; see also http://www.maik.ru.

[Methods of medical and surgical correction of atherosclerotic aorto-arterial pathology in management of malignant neoplasms].

The article deals with 6 -year experience of the Centre of Oncoangiosurgery of the Chelyabinsk Regional Oncological Dispensary in treatment of patients with concomitant malignant neoplasms and atherosclerotic lesions to the aorta and arteries. The authors analyze therapeutic outcomes in a total of 158 patients. Of these, 81 patients were subjected to surgical correction of the blood flow. The presence of a clinically significant atherosclerotic lesion of the aorta and peripheral arteries in a patient should not be interpreted as an absolute or relative contraindication to contraindication combined treatment of the tumour concerned. In the majority of cases, atherosclerotic occlusion of femoral and tibial arteries was treated conservatively, using <>. In manifestation of an atherosclerotic lesion to the carotid arteries, aorta and iliac arteries, surgical correction of the blood flow was used more often. Usually, vascular reconstruction was carried out first. Simultaneous interventions were performed in 16 patients; three patients died. The technique of operations on the aorta and arteries was traditional, while for revascularization in peripheral lesions to the arteries of the limbs more frequently compromising methods were used (sympathectomy, profundoplasty, local endarterectomy) on the background of prostaglandin E1 infusions.

Heavy metal contamination in freshwater fish from the border region between Norway and Russia.

The contents of Cd, Cu, Cr, Hg, Ni and Zn in muscle, liver and gills were studied in whitefish, perch, pike, brown trout, burbot and vendace from three lake localities in a watercourse in the border region between Norway and Russia, in the vicinity of mining activity and several metallurgic smelters. The contents of Cd and Ni in fish tissue increased with increasing proximity to the smelters, whereas the other elements showed similar concentrations at the three localities. The recorded heavy metal concentrations appeared to be within the ranges reported for fish from other metal-contaminated lakes, and higher than comparable observations from unpolluted systems. The heavy metal concentrations were usually lowest in muscle and highest in the liver or the gills. Significant differences in metal concentration levels were found between different fish species, but Hg was the only metal where these species differences were possibly related to biomagnification. For the other elements, the concentrations generally appeared to be inversely related to the trophic level of the fish species.

Impact of airborne pollution on the drainage area of subarctic lakes and fish.

Within the period from 1989 to 1993, the impact of heavy metals and acid oxides on lakes, more than 100 km distant from the nearest source of pollution (enterprises of the copper-nickel industry), has been investigated. On the basis of complex investigations (chemical composition of snowpack and lake sediments, state of fish organisms and populations), it was discovered that there is intensive precipitation of heavy metals and acid oxides within the catchment of the lake Kochejavr. The catchment is characterized by a natural buffer capacity to neutralize acid precipitation. Active accumulation of heavy metals is observed in lake sediments. Metal accumulation causes subtoxic effects on the lake fish. The levels of precipitation of nickel and copper of 0.9 mg/m2 per year over long periods was found to be dangerous for biological systems of freshwater catchments.

[Physico-chemical properties of the complexes of bacitracin with DNA and its effect on the process of transcription in vitro]. / Fiziko-khimicheskie svoistva kompleksov batsitratsina s DNK i vliianie ego na protsess transkriptsii in vitro.

The aim of the present paper was to study the action of one of the peptide antibiotics, bacitracin, as the regulator of gene activity at the transcription level. Therefore the commercial bacitracin has been fractionated into two main parts by paper chromotography. These two fractions have been identified as bacitracin A (biologically active) and bacitracin F (biologically inactive). The binding of both fractions to DNA has been studied. It has been shown that bacitracin A stabilizes DNA to a lesser degree than bacitracin F does. DNA-bacitracin complexes are formed in the major groove of the DNA helix by hydrogen bonds. The analysis of the the obtained experimental data allows us to suppose that bacitracin binding to DNA has a very specific character and that this antibiotic may act as the regulator of gene activity.

[Interaction of the peptide antibiotic bacitracin with DNA and synthetic polynucleotides]. / Issledovanie vzaimodeistviia peptidnogo antibiotika batsitratsina s DNK i sinteticheskimi polinukleotidami.

The aim of the present paper was to study the specific character of interaction of peptide antibiotic bacitracin with DNA and to estimate the interaction constant. The influence of bacitracin on bacteriophage DNA restriction by HindIII and SmaI endonucleases was studied. The possibility of arranging the polynucleotide template by small ligands was shown.

[Role of the ribosomes in controlling cellular differentiation and secondary metabolism in sporulating bacteria. I. Sporogenesis, antibiotic formation and the proteolytic activity of streptomycin-resistant mutants]. / Rol' ribosom v kontrole kletochnoi differentsiatsii i vtorichnogo metabolizma sporoobrazuiushchikh bakterii. Soobshchenie I. Sporogenez, antibiotikoobrazovanie i proteoliticheskaia aktivnost' u streptomitsinustoichivykh mutantov.

A ribosomal mutant Bacillus subtilis IG1 resistant to 100 mkg/ml of streptomycin was isolated. The strA mutation is cotransduced with the cysA gene and, consequently, maps in the ribosomal cluster. The mutation does not influence cell division but does reduce a level of sporulation as well as its antibiotic and proteolytic activity. Involvement of ribosomes in the control of sporulation and secondary metabolism of spore forming bacteria is discussed.

[Participation of temperate phages LP52 and BL20 in the control of bacitracin synthesis in Bacillus licheniformis]. / Izuchenie uchastiia umerennykh fagov LP52 i BL20 v kontrole sinteza batsitratsina u Bacillus licheniformis.

The synthesis of the antibiotic bacitracin in lysogenic and nonlysogenic strains of Bacillus licheniformis 1001 and ATCC10716 has been studied. The antibiotic activity was shown to be about 20% less in lysogens, as compared to nonlysogens. However, the level of bacitracin production was completely restored when temperate bacteriophages BL20 and LP52 were reintroduced into the nonlysogenic strains by virtue of genetic transformation with DNA from lysogenic strains or by transduction with LP52. This may indicate that both phages take part in control of the synthesis of bacitracin. For the time being, the mechanism of regulation is not known. It is likely to be either direct (provided that prophage DNA contains "bacitracin" genes), or indirect.

[A vector for constructing transgenic dicotyledonous plants registering recombination in direct DNA repeats]. / Vektor dlia sozdaniia transgennykh dvudol'nykh rastenii registriruiushchikh rekombinatsiiu po priamym povtoram DNK.

A new pLT-5 vector that can be used for transforming dicotyledonous plants was constructed. Its T-region contains direct repeats of the beta-glucuronidase gene fragment. These repeats are located in such a way that their recombination results in the recovery of gene structure and, therefore, of the gene's ability to be expressed in plant cells.

[Role of the ribosomes in controlling cell differentiation and secondary metabolism in sporulating bacteria. II. The suppression of the phenotypic expression of ribosomal mutations (strA) as affected by RNA-polymerase mutations (rfm) in Bacillus subtilis]. / Rol' ribosom v kontrole kletochnoi differentsiatsii i vtorichnogo metabolizma sporoobrazuiushchikh bakterii. Soobshchenie II. Supressiia fenotipicheskogo vyrazheniia ribosomnykh mutatsii (strA) pod deistviem RNK-polimeraznykh mutatsii (rfm) u Bacillus subtilis.

Ribosomal mutants of Bacillus subtilis IG2 and IG7 resistant to 100 mkg/ml of streptomycin have been isolated. The strA80 and strA88 mutations reduce the level of sporulation as well as antibiotic and proteolytic activity of bacteria. RNA polymerase mutations rfm20 and rfm146 transferred by transformation into ribosomal mutants suppress strA80 and strA88 mutations. As a result, sporulation was restored. Both mutations were cotransformed with cysA gene and mapped within the cluster of ribosomal and RNA polymerase genes. It is proposed that the ribosomal strA80 and strA88 mutations influence transcription of gene (s) which regulate initiation of sporulation and that the RNA polymerase rfm20 and rfm146 mutations can restore sporulation. The results suggest that ribosomes are most likely to be not able to play a specific role in sporulation and secondary metabolism of Bac. subtilis.

[Phenotypic expression of plasmid pPL7065 in Bacillus pumilus in free and integrated states]. / Izuchenie fenotipicheskogo vyrazheniia plazmidy pPL7065 Bacillus pumilus v svobodnom i integrirovannom sostoianii.

There are about 20 copies of pPL7065 plasmid in cells of Bacillus pumilus ATCC7065 with molecular weight of 4.7 X 10(6) daltons. A spontaneous purine dependent mutant LK1 was isolated which contained no autonomous plasmid. However, the presence of the plasmid in LK1 cells was proved by DNA - DNA hybridization. To accomplish this, hybrid plasmid p1G1 was constructed by cloning pPL7065 on a vector pBR322 and multiplying it in Escherichia coli C600. The plasmid then was hybridized with chromosomal DNA of Bac. pumilus LK1. A pur+ revertant has been obtained from LK1 containing autonomous plasmid pPL7065, similar to the parental strain ATCC7065. These results are indicative of pPL7065 integrated into the chromosome of LK1. Bacteria having the autonomous plasmid are oligosporogeneous and produce specific antibiotics. Integration of the plasmid into bacteria causes pur phenotype, normal sporulation and a change in antibiotic synthesis and resistance. LK3 strain which lost pPL7065, sporulates normally and produces the antibiotic that inhibits growth of Bac. pumilus ATCC7065.