Dermal application of nitric oxide in vivo: kinetics, biological responses, and therapeutic potential in humans.

Many local hemodynamic and vascular disorders may be the result of impaired bioavailability of nitric oxide (NO). Previous findings point to a therapeutic potential of dermal NO application in the treatment of hemodynamic disorders, but no reliable data are available on the mechanisms, kinetics, or biological responses relating to cutaneous exposure to NO in humans in vivo. Here we show that, owing to its excellent diffusion capacity, cutaneously applied NO rapidly penetrates the epidermal barrier in significant amounts, strongly enriching skin tissue and blood plasma with its vasoactive derivates. In parallel, it significantly increased vasodilatation and blood flow and reduced thrombocyte aggregation capacity. Data presented here for the first time show that, in humans, dermal application of NO has strong potential for use in the therapy of local hemodynamic disorders arising from insufficient availability of NO or its bioactive derivates.

Mid-infrared cavity leak-out spectroscopy for ultrasensitive detection of carbonyl sulfide.

We present a ringdown absorption spectrometer based on a continuous-wave CO laser in the mid-infrared spectral region near lambda = 5 microm. Using a linear ringdown cavity (length, 0.5 m) with R > = 99.99% mirrors, we observed a noise-equivalent absorption coefficient of 7 x 10(-11) cm(-1) Hz(-1/2). This is 2 orders of magnitude improved compared with previous values. With this setup we studied the spectroscopic detection of carbonyl sulfide (here abbreviated OCS) traces in ambient air and in exhaled breath. We achieved a detection limit of 7 parts in 10(12) (parts per trillion) OCS in ambient air, which is unprecedented and shows great promise for environmental and biomedical applications.

Responses of the infrared sensilla of Melanophila acuminata (Coleoptera: Buprestidae) to monochromatic infrared stimulation.

The pit organs of the beetle Melanophila acuminata were stimulated with monochromatic infrared radiation using a continuous wave CO overtone infrared laser. Best sensitivity was in the wavelength range 2.8-3.5 micron. In this range a stimulus intensity of 14.7 mW cm(-2) was sufficient to generate single action potentials. At a wavelength of 5 microm receptor performance significantly decreased. An increase in stimulus intensity caused a decrease in response latency and an increase in the number of action potentials elicited. At a given wavelength (3.4 microm) the dynamic amplitude range of action potential responses covered 12 dB. At high stimulus intensities (94.2 mW cm(-2)) a stimulus duration of 4 ms was sufficient to generate one to two action potentials and a stimulus duration of 60 ms already caused response saturation (with up to nine action potentials). In a repetitive stimulus regime distinct receptor potentials were visible up to a frequency of 600 Hz.

Ultrahigh-resolution spectrometer for the 5-microm wavelength region.

We present an ultrahigh-resolution saturation spectrometer based on a line-tunable carbon monoxide laser near 60 THz (lambda = 5 microm). A spectral resolution of 14 kHz (Dnu/nu = 2.3 x 10(-10)) for CO fundamental-band transitions was achieved, which improves on earlier results by one order of magnitude. A frequency-locking scheme using tunable microwave sidebands provides tunability and absolute frequency control of the CO laser on the kilohertz. Transit-time broadening and pressure broadening of the observed transitions are significantly reduced by use of expanded laser beams in a 24-m absorption cell at pressures down to 0.0 1Pa. The new spectrometer is suitable for the study of saturation line shapes and the development of a new generation of frequency standards in the 60-THz region.

Division by 3 of optical frequencies by use of difference-frequency generation in noncritically phase-matched RbTiOAsO(4).

A new scheme for coherently connecting optical frequencies in a 3:1 ratio has been demonstrated. To phase lock a Nd:YAG laser at 1064 nm with a CO overtone laser at 3192 nm, we generated their difference frequency in RbTiOAsO(4) (RTA) and beat it against the second harmonic of 3192 nm that was generated in AgGaSe(2).

Real-time detection of 13CH4 in ambient air by use of mid-infrared cavity leak-out spectroscopy.

We report on spectroscopic real-time detection of (13)CH(4) in ambient air. Our measurements were carried out by means of cavity leak-out absorption spectroscopy employing a tunable cw laser in the mid-infrared spectral region near lambda = 3 microm. A CO laser in combination with tunable microwave sideband generation was used as the light source. Using a 50-cm-long ringdown cell with R = 99.98% mirrors, we achieved a detection limit of 290 parts in 10(12) (ppt) (13)CH(4) in ambient air (integration time, 100 s). The corresponding noise-equivalent absorption coefficient was 5 x 10(-9)/cm.

Tunable carbon monoxide overtone laser sideband system for precision spectroscopy from 2.6 to 4.1 microm.

We report a source of tunable laser radiation for high-precision molecular spectroscopy in the 2.6- 4.1-microm spectral region. Laser light from a CO overtone laser is mixed with microwaves, generating tunable sidebands of ~1 mW of power. We achieve very high absolute frequency accuracy by frequency-offset locking the CO laser to a CO(2) laser secondary frequency standard. The uncertainty of the laser frequency is less than 30 kHz (Dnu/nu=3x10(-10)) , and the laser linewidth is of the order of 100 kHz. This tunable and ultrastable laser system is suitable for very accurate molecular spectroscopy and metrology in a most interesting wavelength region. We demonstrate an application of the system to saturated-absorption spectroscopy of a rovibrational transition of carbonyl sulfide.

More Sub-Doppler Heterodyne Frequency Measurements on OCS between 56 and 63 THz.

By using tunable microwave sidebands added to CO-laser lines, we have made more sub-Doppler heterodyne frequency measurements on OCS. Three new rotational transitions have been measured for each of three absorption bands, 10(0)0-00(0)0, 02(0)1-00(0)0, and 03(1)1-01(1)0. The absolute uncertainties of the measurements are on the order of +/-25 kHz. New calibration tables are given for the region 1860-1925 and 2020-2085 cm(-1) based on the most recent OCS measurements. Copyright 2000 Academic Press.

Sub-Doppler Heterodyne Frequency Measurements on OCS Near 2900 cm-1 Using a CO Overtone Sideband Spectrometer.

We present sub-Doppler heterodyne frequency measurements on 10 rovibrational transitions of carbonyl sulfide (OCS) between 2894 and 2910 cm-1. The measurements were made using a CO overtone laser which had limited tuneability through the generation of microwave sidebands in a CdTe crystal. With this technique the laser frequencies were shifted to the desired OCS transition frequencies. The transition frequencies could be measured with uncertainties less than 30 kHz (Deltanu/nu = 3 x 10(-10)) by frequency offset-locking the CO overtone laser to combination frequencies of two saturation-stabilized CO2 laser standards. The measured transition frequencies of OCS were combined with previous sub-Doppler, Fourier transform, and microwave measurements to recalculate improved calibration tables for the 10(0)1-00(0)0, 11(1e)1-01(1e)0, and 11(1f)1-01(1f)0 bands. These tables are suitable for the calibration of infrared spectrometers in the 87 THz region (near 2900 cm-1). Copyright 1998 Academic Press.