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PURPOSE: Significant advancements in improving ovarian cancer (OC) outcomes have been limited over the past decade. To predict prognosis and improve outcomes of OC, we plan to develop and validate a robust prognosis signature based on blood features. METHODS: We screened age and 33 blood features from 331 OC patients. Using ten machine learning algorithms, 88 combinations were generated, from which one was selected to construct a blood risk score (BRS) according to the highest C-index in the test dataset. RESULTS: Stepcox (both) and Enet (alpha = 0.7) performed the best in the test dataset with a C-index of 0.711. Meanwhile, the low RBS group possessed observably prolonged survival in this model. Compared to traditional prognostic-related features such as age, stage, grade, and CA125, our combined model had the highest AUC values at 3, 5, and 7 years. According to the results of the model, BRS can provide accurate predictions of OC prognosis. BRS was also capable of identifying various prognostic stratifications in different stages and grades. Importantly, developing the nomogram may improve performance by combining BRS and stage. CONCLUSION: This study provides a valuable combined machine-learning model that can be used for predicting the individualized prognosis of OC patients.
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Nomogramas , Neoplasias Ováricas , Humanos , Femenino , Adulto , Pronóstico , Neoplasias Ováricas/diagnóstico , Neoplasias Ováricas/cirugía , Algoritmos , Aprendizaje AutomáticoRESUMEN
BACKGROUND: Inflammation exerts a critical role in the pathogenesis of infertility. The relationship between inflammatory parameters from peripheral blood and infertility remains unclear. Aim of this study was to investigate the association between inflammatory markers and infertility among women of reproductive age in the United States. METHODS: Women aged 20-45 were included from the National Health and Nutrition Examination Survey (NHANES) 2013-2020 for the present cross-sectional study. Data of reproductive status was collected from the Reproductive Health Questionnaire. Six inflammatory markers, systemic immune inflammation index (SII), lymphocyte count (LC), product of platelet and neutrophil count (PPN), platelet-lymphocyte ratio (PLR), neutrophil-lymphocyte ratio (NLR) and lymphocyte-monocyte ratio (LMR) were calculated from complete blood counts in mobile examination center. Survey-weighted multivariable logistic regression was employed to assess the association between inflammatory markers and infertility in four different models, then restricted cubic spline (RCS) plot was used to explore non-linearity association between inflammatory markers and infertility. Subgroup analyses were performed to further clarify effects of other covariates on association between inflammatory markers and infertility. RESULTS: A total of 3,105 women aged 20-45 was included in the final analysis, with 431 (13.88%) self-reported infertility. A negative association was found between log2-SII, log2-PLR and infertility, with an OR of 0.95 (95% CI: 0.78,1.15; p = 0.60), 0.80 (95% CI:0.60,1.05; p = 0.10), respectively. The results were similar in model 1, model 2, and model 3. Compared with the lowest quartile (Q1), the third quartile (Q3) of log2-SII was negatively correlation with infertility, with an OR (95% CI) of 0.56 (95% CI: 0.37,0.85; p = 0.01) in model 3. Similarly, the third quartile (Q3) of log2-PLR was negatively correlation with infertility, with an OR (95% CI) of 0.61 (95% CI: 0.43,0.88; p = 0.01) in model 3. No significant association was observed between log2-LC, log2-PPN, log2-NLR, log2-LMR and infertility in model 3. A similar U-shaped relationship between log2-SII and infertility was found (p for non-linear < 0.05). The results of subgroup analyses revealed that associations between the third quartile (Q3) of log2-SII, log2-PLR and infertility were nearly consistent. CONCLUSION: The findings showed that SII and PLR were negatively associated with infertility. Further studies are needed to explore their association better and the underlying mechanisms.
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Infertilidad , Inflamación , Femenino , Humanos , Estudios Transversales , Infertilidad/epidemiología , Inflamación/epidemiología , Encuestas Nutricionales , Estudios Retrospectivos , Adulto Joven , Adulto , Persona de Mediana EdadRESUMEN
Heat stress poses a threat to plants in arid and semiarid regions, leading to soil salinization and plant mortality. Researchers are exploring remedies to alleviate these effects, including using gibberellic acid (GA3) to regulate plant enzymes and antioxidants. Additionally, sodium nitroprusside (SNP) is gaining attention, but its combined effect with GA3 requires further research. To address this gap, we investigated the effects of GA3 and SNP on plants under heat stress conditions. For that, wheat plants were cultivated under 40 °C for 6 h per day (15 days). Sodium nitroprusside (donor of NO and SNP) and gibberellic acid (GA3), respectively, with 100 µM and 5 µg/ml concentrations, were applied as foliar sprays at 10 days after sowing (DAS). Results showed that SNP + GA3 treatment had the highest plant height (4.48% increase), plant fresh weight (29.7%), plant dry weight (87%), photosynthetic rate (39.76%) and stomatal conductance (38.10%), and Rubisco (54.2%) compared to the control. Our findings indicate a significant increase in NO, H2O2, TBARS, SOD, POD, APX, proline, GR, and GB that greatly scavenged reactive oxygen species (ROS) for decreasing the adverse effect of stress. Such findings confirmed the efficacy of the combined treatment of SNP + GA3 under high-temperature stress compared to the solitary application of GA3, SNP, and control. In conclusion, using SNP + GA3 is a better strategy for mitigating heat stress in wheat than individual applications. Further research is recommended to validate the effectiveness of SNP + GA3 in other cereal crops.
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Peróxido de Hidrógeno , Triticum , Nitroprusiato/farmacología , Triticum/fisiología , Peróxido de Hidrógeno/farmacología , Respuesta al Choque TérmicoRESUMEN
OBJECTIVE: To construct a novel targeted drug delivery nanoprobe: iodine-131-arginine-glycine-aspartate-tyrosine-cysteine peptide-polyethylene glycol-fifth generation polyamide-amine-docetaxel (131I-RGDyC-PEG-PAMAM-DTX) and to investigate its physicochemical properties and biological activity. MATERIALS AND METHODS: Docetaxel was wrapped by solvent volatilization method, and the regression curve of DTX was constructed by high-performance liquid chromatography to determine its drug loading. The particle size of RGDyC-PEG-PAMAM-DTX was detected by dynamic light scattering. The 131I labeling was performed by a chloramine-T method and purified by Sephadex-G50 column chromatography, and it is in vitro stability and lipid-water partition coefficient was investigated. The cytotoxicity of RGDyC-PEG-PAMAM-DTX and 131I-RGDyC-PEG-PAMAM-DTX on A549 cells in vitro was detected by Cell Counting Kit-8 assay. RESULTS: Arginine-glycine-aspartate-tyrosine-cysteine peptide-PEG-PAMAM-DTX was successfully prepared by solvent volatilization with a loading capacity of about 44µg/mg. The average particle size of RGDyC-PEG-PAMAM-DTX was 57.8nm; the labeling rate of 131I-RGDyC-PEG-PAMAM-DTX by the chloramine-T method was 74.09%-76.09%, and the radiochemical purity was 88.9%-92.6% after purification. The in vitro stability showed that the radiochemical purity was above 80% after 72h in fetal bovine serum and PBS buffer (25oC and 37oC).CCK-8 assay showed that RGDyC-PEG-PAMAM-DTX and 131I-RGDyC-PEG-PAMAM-DTX had more pronounced cytotoxic effects than free DTX and 131I. CONCLUSION: Iodine-131-RGDyC-PEG-PAMAM-DTX has good physicochemical properties and apparent cytotoxic effectsandis expected to be used in treating tumors.
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Antineoplásicos , Ácido Aspártico , Humanos , Docetaxel/uso terapéutico , Cisteína , Línea Celular Tumoral , Sistemas de Liberación de Medicamentos , Polietilenglicoles/química , Arginina , Solventes , Péptidos , Glicina , TirosinaRESUMEN
Thyroid hormone was involved in gene expression and functional regulation in various signal pathways. Cold stress can increase triiodothyronine (T3) level in the blood. The aim of this study was to investigate the effect of T3 on HSP70 expression and apoptosis in Sertoli cells (SCs) under cold stress in vitro culture at 26 °C, and provide a theoretical and practical basis for improving the reproductive efficiency of bulls in cold areas. SCs were treated with different cold stress duration and different T3 concentrations for pre-screening. HSP70 inhibitor was added later, and the apoptotic rate was measured using flow cytometry. The expression of HSP70 and the main genes of mitochondrial apoptosis pathway were determined by means of real-time PCR and western-blot, respectively. The localization of HSP70 was assessed by immunofluorescence. The results showed that cold stress (26 °C, 6 h) played an inductive role in SCs apoptotic rate (P < 0.01) and the transfer of HSP70 into the nucleus. 100 nM T3 further promoted HSP70 expression and its transfer into the nucleus, which significantly inhibited the expression of vital genes (cyt-c, Caspase-9 and Caspase-3) in mitochondrial pathway (P < 0.05). Subsequently, higher survival and lower apoptotic rates of SCs (P < 0.01) were observed. When T3 and HSP70 inhibitor were added together, the expression of cyt-c, Caspase-9 and Caspase-3 were inhibited (P < 0.05), and then the declining apoptotic rate increased again (P < 0.01). In conclusion, T3 can regulate HSP70 expression and translocation to mediate mitochondrial apoptosis pathway to inhibit SCs apoptosis induced by cold stress.
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Respuesta al Choque por Frío , Células de Sertoli , Animales , Apoptosis , Caspasa 3/metabolismo , Caspasa 9 , Bovinos , Criopreservación/métodos , Citocromos c/metabolismo , Proteínas HSP70 de Choque Térmico/genética , Proteínas HSP70 de Choque Térmico/metabolismo , Masculino , Transducción de Señal , Hormonas Tiroideas/metabolismo , Hormonas Tiroideas/farmacología , Triyodotironina/metabolismo , Triyodotironina/farmacologíaRESUMEN
Proper amounts of copper supplemented in livestock feed improve the physical growth and traits of farm animals. The pancreas is an important organ with both exocrine and endocrine portions. To investigate the role and mechanism of copper in the sheep pancreas, we first established sheep pancreatic duct organoids (sPDOs). We found that an appropriate amount of copper benefited the formation and growth of sPDOs, whereas excess or deficient copper damaged sPDOs. We found that the proliferation-stimulating effect of copper was related to the copper chaperone antioxidant protein 1 (ATOX1)-dependent activation of MEK-ERK1/2 signaling. Atox1 knockdown suppressed the cell proliferation of sPDOs, even in the presence of the MEK activator. These results indicate that moderate concentrations of copper promote sPDO growth through ATOX1-regulated cell proliferation by activation of MEK-ERK. Moreover, our study indicates that organoids may be a useful model to study organ growth mechanisms in livestock.
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Cobre/farmacología , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Conductos Pancreáticos/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Animales , Antioxidantes/metabolismo , Antioxidantes/farmacología , Proteínas de Transporte de Catión/metabolismo , Proliferación Celular/efectos de los fármacos , Cobre/metabolismo , Proteínas Transportadoras de Cobre/metabolismo , Organoides/metabolismo , Conductos Pancreáticos/metabolismo , OvinosRESUMEN
The gap junction protein connexin (Cx) 43 between adjacent Sertoli cells (SCs) is the main testicular factor regulating the growth and development of SCs, and plays a vital role in controlling cell differentiation and maturation. However, the endogenous testicular factors that regulate Cx43 and the downstream signalling pathways that mediate Cx43-dependent SC differentiation are unclear. In this study, high-purity SCs were isolated from newborn calves' testes by differential adherence. The SCs were then cultured invitro and treated with short interference RNA to knockdown endogenous Wilms' tumour 1 (WT1). In WT1-knockdown SCs, Cx43 expression was upregulated. To elucidate the intracellular signalling mechanism of Cx43 in the differentiation and maturation of immature SCs, SCs were treated simultaneously with non-canonical Wnt signalling inhibitors CCG-1423 and GO-6983; in these SCs, Cx43 expression was upregulated. Together, these data indicate that WT1 negatively regulates the expression of Cx43 produced from SCs via a non-canonical Wnt signalling pathway in cultured bovine SCs.
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Conexina 43/metabolismo , Células de Sertoli/metabolismo , Proteínas WT1/metabolismo , Vía de Señalización Wnt , Animales , Bovinos , Diferenciación Celular , Células Cultivadas , Conexina 43/genética , Regulación de la Expresión Génica , Masculino , Proteínas WT1/genéticaRESUMEN
Pancreatic stem/progenitor cells convert from a proliferative to a differentiated fate passing through proliferation cease to a resting state. However, the molecular mechanisms of cell cycle arrest are poorly understood. In this study, we demonstrated that the microRNA-124a (miR-124a) inhibited the proliferation of pancreatic progenitor cells both in vitro and ex vivo and promoted a quiescent state. The miR-124a directly targeted SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1), IQ motif-containing GTPase-activating protein 1 (IQGAP1), signal transducer and activator of transcription 3 (STAT3), and cyclin D2 (CCND2), thereby inactivating epidermal growth factor receptor (EGFR) downstream signaling pathways including mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK/ERK), phosphatidylinositol 3-kinase-protein kinase B (PI3K/AKT) and Janus kinase (JAK)/STAT3. miR-124a blocked cell proliferation mainly through targeting STAT3 to inhibit PI3K/AKT and JAK/STAT3 signaling. Moreover, miR-124a expression was negatively regulated by EGFR downstream PI3K/AKT signaling. These results indicated that miR-124a and EGFR signaling mutually interact to form a regulating circuit that determines the proliferation of pancreatic progenitor cells.
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Diferenciación Celular/genética , Proliferación Celular/genética , MicroARNs/genética , Fosfatidilinositol 3-Quinasas/genética , Transducción de Señal , Células Madre/citología , Animales , Receptores ErbB/metabolismo , Regulación Neoplásica de la Expresión Génica/genética , Ratones , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal/genética , Células Madre/metabolismoRESUMEN
The essential trace element zinc (Zn) is widely required in cellular functions, and abnormal Zn homeostasis causes a variety of health problems including immunodeficiency and sensory dysfunctions. Previous studies had shown that Zn availability was also important for tumor growth and progression. The aim of the present study was to investigate the potential mechanisms of N,N,N,N-Tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN) (a membrane permeable zinc chelator) induced pancreatic cancer cell death. The text of inductively coupled plasma-mass spectrometry (ICP-MS) showed in human pancreatic cancer samples that the zinc content in cancer was higher than that in adjacent tissues. The pancreatic cancer cell lines Panc-1, 8988T, BxPc-3, and L3.6 were used in this study. Our results indicated that TPEN markedly induced cell death, via increasing reactive oxygen species (ROS) and restraining autophagy. Our data also indicated that TPEN-stimulated mitochondrial metabolism produced much ROS. Meanwhile, TPEN reduced the levels of glutathione (GSH) and triggered ROS outbreak, which were the main causes of cell death. In addition, cell autophagy was significantly depressed in Panc-1 cells treated by TPEN, which was due to the ability of disrupting lysosomal by TPEN. Thus, we thought zinc depletion by TPEN was a potential therapeutic strategy for pancreatic cancer.
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Autofagia/efectos de los fármacos , Quelantes/farmacología , Etilaminas/farmacología , Estrés Oxidativo/efectos de los fármacos , Neoplasias Pancreáticas/tratamiento farmacológico , Piridinas/farmacología , Zinc/metabolismo , Antineoplásicos/farmacología , Carcinoma , Línea Celular Tumoral , Proliferación Celular , Supervivencia Celular/efectos de los fármacos , Quelantes/química , Relación Dosis-Respuesta a Droga , Etilaminas/administración & dosificación , Femenino , Humanos , Piridinas/administración & dosificación , Especies Reactivas de Oxígeno , Neoplasias del Cuello Uterino/tratamiento farmacológicoRESUMEN
Traditional laser-induced breakdown spectroscopy (LIBS) always fails to directly detect target in aqueous solution due to rapid quenching of emitted light and adsorption of pulse energy by surrounding water. A method is proposed for the in situ underwater LIBS analysis of Cr(VI) in aqueous solution freed from the common problems mentioned above by combining a gas-assisted localized liquid discharge apparatus with electrosorption for the first time. In this approach, the introduction of the gas-assisted localized liquid discharge apparatus provides an instantaneous gaseous environment for underwater LIBS measurement (that is, the transfer of sampling matrix is not needed from aqueous solution to dry state). The preconcentration of Cr(VI) is achieved by electrosorption with a positive potential applied around adsorbents, which can promote the adsorption of Cr(VI) and inhibit that of the coexisting cations leading to a good anti-interference. Amino groups functionalized chitosan-modified graphene oxide (CS-GO) is utilized for Cr(VI) enrichment, which can be protonated to form NH3+ in acidic condition promoting the adsorption toward Cr(VI) by electrostatic attraction. The highest detection sensitivity of 5.15 counts µg-1 L toward Cr(VI) is found for the optimized electrosorption potential (EES = 1.5 V) and electrosorption time (tES = 600 s) without interference from coexisting metal ions. A corresponding limit of detection (LOD) of 12.3 µg L-1 (3σ method) is achieved, which is amazingly improved by 2 or even 3 orders of magnitude compared to the previous reports of LIBS.
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In this work, a mobile laser-induced breakdown spectroscopy (LIBS) system has been successfully applied to in situ analysis of heavy metals in soil samples. The LIBS system had two working methods, which were a fixed measuring method and a handheld method. For the fixed measuring method, a simple sample pretreatment was needed to reduce the soil matrix effect generated by moisture and porosity. Experiments proved that this method could be used to semi-quantitatively detect heavy metals when combined with the traditional calibration curve method. The limits of detection for copper, lead, and zinc were all below 10 mg/kg, which satisfied the need of heavy metal detection in soil. Principal component analysis was used for soil classification, which helped to build appropriate calibration curves. On the basis of soil classification, accurate and rapid detection of heavy metals in soil is feasible. For the handheld method, spectrum intensity and stability decreased significantly compared with the fixed measuring method. However, by using the internal standard method, the stability of LIBS data was improved significantly to 6%. For soil samples with serious heavy-metal pollution, the measurement errors were less than 12%, which indicated that handheld LIBS was effective to monitor heavy-metal pollution in soil. The research results provide application support for rapid and on-site monitoring of heavy metals in soil.
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In order to improve the detection sensitivity and spectral characteristic of laser-induced breakdown spectroscopy (LIBS), re-heating orthogonal dual-pulse configuration is adopted to analyze Fe, Pb, Ca and Mg contained in the sample and soil sample contained different concentrations of heavy mental Cr. Variation relationship between spectral intensity, signal-to-background(SBR) of four characteristic spectral lines Feâ ï¼404.581 nmï¼Pbâ ï¼405.78 nmï¼Caâ ï¼422.67 nm and Mgâ ï¼518.361 nm and time interval of two laser pulses is discussed, the best time interval of two laser pulses is obtained 1.0 µs. In the condition of single pulse and dual-pulse, the enhancement factor of spectral intensity of four characteristic spectral lines Feâ ï¼404.581 nmï¼Pbâ ï¼405.78 nmï¼Caâ ï¼422.67 nm and Mgâ ï¼518.361 nm is respectively 2.23ï¼2.31ï¼2.42 and 2.10; The time evolution characteristic of spectral intensity of characteristic spectral lines Feâ ï¼404.581 nm and Caâ ï¼422.67 nm is considered, and also the variation relationship between spectral acquisition delay time and SBR of four characteristic spectral lines, dual-pulse can prolong decay time of spectral intensity and improve the SBR of characteristic spectral lines; time evolution characteristic of plasma temperature and electron density is compared in the condition of single pulse and dual-pulse, maximum elevation of plasma temperature is found to be 730 K, and the maximum increase of electron density is 1.8×1016 cm-3. The limits of detection of heavy mental Cr are obtained 38 and 20 µg·g-1 respectively in condition of single and double pulse, limit of detection of Cr is reduced approximately 2 times by the condition of re-heating orthogonal dual pulse. Results above indicate that re-heating orthogonal dual-pulse can improve detection sensibility and spectral characteristic of LIBS technique, which provides an effective method for decreasing the limit of detection of elements.
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In order to improve the detection sensitivity of laser-induced breakdown spectroscopy (LIBS) and lower the limit of the detection of elements, LIBS combined with Aluminum electrode enrichment method is adopted to analyze heavy metals such as Pb,Cdand Ni in the water. The relationship between the characteristic spectral intensity and the key parameters-voltage of electrode method is discussed, the spectral intensity increases first and then decreases with the increase of voltage. The spectral intensity reaches the maximum value when the enrichment voltage is 1.2 V while the optimal enrichment voltage value is 1.2 V. The stability of characteristic spectral lines of heavy metals is studied, and the relative standard deviation(RSD) of spectral intensity of Pb, Cd and Ni is 5.98ï¼ ï¼4.25 ï¼ and 5.27ï¼ respectively, the result shows that the spectral line obtained by this method has high stability. A series of samples in the range of 0~0.13 mg·L-1 are prepared and quantitatively analyzed, the limit of detection of Pb, Cd and Ni is obtained 1.2ï¼3.1 and 1.7 ppb respectively. The above result shows that LIBS combined with aluminum electrode enrichment method can effectively improve the stability of characteristic spectral lines and lower the limit of detection of Pb,Cd and Ni. This research also provides a method to further improve detection sensitivity of LIBS and analysis ability of heavy metal in the water.
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Soil classification is an important research content in soil science field. It is the basis of soil survey and resource evaluation which is important to agricultural production. There are many soil classification standards all over the world. China has two kinds of classifications including soil genetic classification and soil system classification. There are great differences between different types of soil elements, so it is feasible for soil classification to use laser induced breakdown spectroscopy. Laser induced breakdown spectroscopy (LIBS) is a new element analysis technology which uses a laser pulse with high energy density to ablate samples. LIBS has been used in many fields including environmental protection and industrial production control. It can directly reflect the difference of element content in different soils. The experimental setup including an Nd: YAG laser, a spectrometer, a computer and a rotating platform. In the experiment 7 kinds of soil (red soil, brick red soil, lateritic red soil, paddy soil, cinnamon, alluvial soil and alpine meadow soil) including 25 samples were used. All soil samples were grinded and sieved before the experiment. Under the same experimental condition, the temperatures of the plasma created by the laser pulses on the surface of the different soil samples have great differences. The lateritic red soil had the highest temperature, and the alpine meadow soil had the lowest. But it was not enough to form the basis for classification. Therefore six constant elements including Si, Fe, Al, Mg, Ca and Ti were selected and their spectral line intensity were treated as classification index. Principal component analysis (PCA) was used to simplify the classification process. The PCA method could simplify the 6 indexes to few independent indexes which could also reflect the spectral information of the 6 elements. The original spectral data was processed by Matlab. The process consisted of spectral background removal, characteristic spectrum identify and extraction. The classification results showed a three--dimensional figure. Except alpine meadow soil which varied in element concentrations 6 kinds of soils achieved good classification. The brick red soil and lateritic red soil varied in PC1, but their PC2s and PC3s were the same. The two kinds of soil overlapped with each other and they couldn't be separated. Back-propagation artificial network was also used to achieve soil classification. The classification results were the same with the PCA. Some brick red soil and lateritic red soil samples were identified inaccurately. When the PC1, 2, 3 were used as the input of the BP-neural network, the classification had much better accuracy because less input improved the performance of the BP-neural network. Only one alpine meadow soil sample was identified to cinnamon soil. When the plasma temperature was also taken into account, all the soil samples could be distinguished. The results showed that LIBS could be used to classify soils based on their element content differences. The PCA, soil plasma temperature and BP-neural network were useful tools to achieve soil classification. The LIBS provides a useful tool for general detailed soil survey and rational utilization of soil.
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The structures of bacterial cells are analyzed in this paper. The scattering components of individual cell were divided into two parts including external structure and internal structure. The interpretation model of bacteria about scattering light is established. The model is used to analyze the scattering light of Escherichia coli in the region of 400~900 nm. The average size of external structure and the internal structure can be obtained, and the ratio of the two parts is also obtained. According to the relationship of the optical density of single cell and the overall measurement, the concentration of bacterial can be obtained quickly. The maximum difference in all the concentrations of the bacteria repeated measurements is 1.83%; compared with the plate culture method, the measurement results were in the same order of magnitude, with relative error of 3.43%. The scattering light of Escherichia coli and Klebsiella pneumoniae are analyzed in different growth stages, the curves of the concentration and the size of the two species bacteria over time are obtained. The results can provide a quick way for the study of bacterial growth and technical support for rapid detection of bacteria in the water.
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Bacterias , Escherichia coli , Klebsiella pneumoniae , Dispersión de Radiación , Agua , Microbiología del AguaRESUMEN
Polycyclic Aromatic Hydrocarbons (PAHs) have been widely investigated in environmental field, for most of them are mutagenic (carcinogenic, teratogenic, mutagenic). The influence of delay time and gate width on the time-resolved fluorescence spectroscopy of fluoranthene in ethanol was studied in this paper. Furthermore, laser induced time-resolved fluorescence spectroscopy of fluoranthene with different concentration were also researched. According to the results, fluorescence kinetics decay curves and fluorescence lifetime of fluoranthene matched. The research results showed that there was closely relationship between the fluorescence spectrum of fluoranthene and the delay time and gate width of detector. When the delay time was fixed, the fluorescence intensity of fluoranthene grew increased as the gate width increased. When the gate width was fixed, the fluorescence intensity of fluoranthene increased first and then decreased with the delay time increases. The process of the attenuation of fluorescence intensity of fluoranthene with time delay conformed to the exponential decay process. The stepwise dilution of fluoranthene ethanol solution was also studied. With increasing dilution, the fitting of fluoranthene fluorescence lifetime increased. The results of this paper can provide a technical basis for the detection of PAHs in the environment, due to the different characteristics of the fluorescent substance having fluorescence lifetime.
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Extensive use of pesticides has a significant impact on the environment. Carbaryl, whose residues stay in the surface water, is one of the most widely used broad spectrum insecticides in agriculture. It is important to understand carbaryl spectral characteristics and detection methods. The characteristic of excitation-emission three-dimensional spectra of carbaryl is studied. By changing the concentration of methanol in methanol-water binary solvent, the impact of methanol-water mixture on three-dimensional fluorescence spectra of carbaryl is discussed. The results show that the characteristic excitation-emission spectra of carbaryl is single peak, the range of the excitation wavelength and emission wavelength are: 244~304 and 300~350 nm respectively, the maximum excitation/emission peak located at 280 and 335 nm. With increasing the content of methanol in methanol-water binary solvent mixture, there is no obviously spectra shift of three dimensional fluorescence spectra of carbaryl. However, the intensity of fluorescence is nonlinear dependent on the content of methanol, mainly due to the specific properties of binary mixed solvent.
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Laser-induced breakdown spectroscopy (LIBS) was used to calibrate the concentration of Cr in soils combined with Support Vector Machine. The Nd:YAG pulse laser with the wavelength of 1 064 nm was used as the excitation source. The grating spectrometer and the charge couple device were used as spectral separation device and the spectral detection device. The multiple linear regression and support vector machine were adopted to make quantitative analysis on Cr in soils respectively. The result indicate that the multiple linear regression can get more accurate informination of the spectral lines: the correlation coefficient is increased from 0.689 to 0.980 compared with conventional quantitative method. Thereofre, the the accuracy of quantitative analysis is increased. The slope about calibration curve with support vector machine of test set is nearly about 1 and the correlation coefficient is 0.998, the relative errors for the test set all are lower than 2.57%, the quantitative analysis results about support vector machine are better than the results combined with the conventional quantitative method and the multiple linear regression. The support vector machine can correct the matrix effect and improve the accuracy of prediction on the concentration of Cr in soil.
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Enfermedad Celíaca/diagnóstico , Enfermedades Intestinales/diagnóstico , Intestino Delgado/inmunología , Trastornos Linfoproliferativos/diagnóstico , Linfocitos T/inmunología , Biopsia , Enfermedad Celíaca/dietoterapia , Enfermedad Celíaca/inmunología , Enfermedad Celíaca/patología , Errores Diagnósticos , Dieta Sin Gluten , Duodenoscopía , Glucocorticoides/uso terapéutico , Humanos , Inmunohistoquímica , Enfermedades Intestinales/tratamiento farmacológico , Enfermedades Intestinales/inmunología , Enfermedades Intestinales/patología , Intestino Delgado/efectos de los fármacos , Intestino Delgado/patología , Trastornos Linfoproliferativos/tratamiento farmacológico , Trastornos Linfoproliferativos/inmunología , Trastornos Linfoproliferativos/patología , Masculino , Microscopía Confocal , Persona de Mediana Edad , Valor Predictivo de las Pruebas , Linfocitos T/efectos de los fármacos , Linfocitos T/patología , Resultado del TratamientoRESUMEN
There are many influence factors in the precision and accuracy of the quantitative analysis with LIBS technology. According to approximately the same characteristics trend of background spectrum and characteristic spectrum along with the change of temperature through in-depth analysis, signal-to-background ratio (S/B) measurement and regression analysis could compensate the spectral line intensity changes caused by system parameters such as laser power, spectral efficiency of receiving. Because the measurement dates were limited and nonlinear, we used support vector machine (SVM) for regression algorithm. The experimental results showed that the method could improve the stability and the accuracy of quantitative analysis of LIBS, and the relative standard deviation and average relative error of test set respectively were 4.7% and 9.5%. Data fitting method based on signal-to-background ratio(S/B) is Less susceptible to matrix elements and background spectrum etc, and provides data processing reference for real-time online LIBS quantitative analysis technology.