RESUMEN
Aged individuals are more susceptible to hypoxic insults, but little is known about the response of the nitric oxide (NO) system to hypoxia in the senescent brain. We have analysed the effect of aging on the hypobaric hypoxia/reoxygenation NO synthase (NOS) expression and activity in the cerebral cortex. In aged animals, the absence of significant changes in NOx and activity indicates a weaker response of the systems involving NO production in this pathological situation. The nNOS protein levels remained invariable and similar in both age groups after hypoxia, although in aged animals the mRNA did not change and was consistently lower than in adults. Both eNOS mRNA and protein increased shortly after hypoxia. However, although eNOS protein levels were quite similar in both age groups, the increase appeared later and was less persistent in aged animals. Real-time RT-PCR revealed a similar basal inducible NOS (iNOS) mRNA expression that responded late in reoxygenation, mainly in aged rats. However, neither iNOS protein nor activity was detected in any age group. Altogether our results indicate that aging attenuates the response of the NO system to a hypoxic injury, particularly at eNOS level, the activity of which is crucial for maintaining vascular homeostasis.
Asunto(s)
Envejecimiento/metabolismo , Corteza Cerebral/metabolismo , Hipoxia/metabolismo , Óxido Nítrico/metabolismo , Animales , Expresión Génica , Masculino , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico/antagonistas & inhibidores , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico Sintasa de Tipo I/análisis , Óxido Nítrico Sintasa de Tipo I/genética , Óxido Nítrico Sintasa de Tipo II/análisis , Óxido Nítrico Sintasa de Tipo II/genética , Óxido Nítrico Sintasa de Tipo III/análisis , Óxido Nítrico Sintasa de Tipo III/genética , ARN Mensajero/análisis , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
BACKGROUND: Cardiovascular disease is the leading cause of death in the Western world, and a major cause of this disease is atherosclerosis. Research has demonstrated that pregnancy-associated plasma protein A (PAPP-A) plays a role in cardiovascular disease, as evidenced by the association between PAPP-A and severity of heart damage. AIM: The aim of this work was to investigate the correlation between PAPP-A concentrations in coronary and peripheral blood and certain clinicopathological factors and antioxidant enzyme activities in patients diagnosed with coronary artery disease. METHODS: For 65 patients, arterial blood was obtained by puncturing the femoral or radial artery, and coronary blood was obtained via percutaneous coronary intervention. PAPP-A, catalase (CAT), superoxide dismutase-1 (SOD-1), and superoxide dismutase-2 (SOD-2) levels were measured using spectrometric methods. RESULTS: Coronary PAPP-A levels were slightly higher than peripheral PAPP-A levels (81.25 ± 2.34 and 62 ± 3 ng/mL, respectively, P < 0.0001); these levels were correlated with each other (r = 0.6629, P < 0.001) but not with clinicopathological factors (P > 0.05). Coronary PAPP-A levels were significantly elevated among patients at risk for cardiovascular disease (P < 0.05). Antioxidant enzyme activities were significantly higher in coronary samples than in peripheral samples from subjects with ischemic cardiopathy secondary to atherosclerosis (P < 0.001). Neither coronary nor peripheral PAPP-A levels were correlated with antioxidant enzyme activities in patients with cardiopathy secondary to atherosclerosis (P > 0.05). CONCLUSIONS: PAPP-A levels could be used as biomarkers to identify patients at risk of coronary artery disease.
Asunto(s)
Biomarcadores/sangre , Enfermedad de la Arteria Coronaria/sangre , Proteína Plasmática A Asociada al Embarazo/metabolismo , Anciano , Anciano de 80 o más Años , Enfermedad de la Arteria Coronaria/patología , Humanos , Persona de Mediana EdadRESUMEN
The effect of aging on basal and hypoxia/reoxygenation levels of both oxidative stress (protein carbonyl and TBARS) and antioxidative-enzyme activity (Cu/Zn-SOD; Mn-SOD; Catalase, CAT; Se-independent and Se-dependent glutathione peroxidase, GPX; glutathione transferase, GST and glutathione reductase, GR) has been studied in the cerebral cortex of adult and old rats. Oxidative stress markers increased with aging and show an age-dependent post-hypoxic response. Moreover, aging caused either no change (GST, GR and CAT) or an increase (Se-GPX, Cu/Zn-SOD, Mn-SOD) in the basal activity of the enzymes analysed. Only Se-independent GPX activity decreases. However, we detected an age-dependent response of SODs to the hypoxic injury. The early and sustained Cu/Zn-SOD activity rise in adult animals became late and weak in aged animals. Meanwhile, aging slowed the Mn-SOD post-hypoxic response although this activity was consistently higher in aged rats. Aging eliminated the post-hypoxic CAT response, but, perhaps offset by increased GPX activity, did not affect the GST response and slightly reduced post-hypoxic GR activity. In conclusion, aging rise basal ROS production, does not diminish or even increase the antioxidative-enzyme activity, and may slow but does not usually eliminate the enzymatic antioxidant response to the increased post-hypoxic ROS generation.
Asunto(s)
Envejecimiento/fisiología , Corteza Cerebral/enzimología , Hipoxia Encefálica/fisiopatología , Animales , Antioxidantes/metabolismo , Hipoxia Encefálica/enzimología , Peroxidación de Lípido , Masculino , Oxidación-Reducción , Estrés Oxidativo , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/metabolismoRESUMEN
Aged brain shows reduced biological plasticity to meet emergency conditions such as ischemia, a process in which nitric oxide (NO) and apoptosis have been shown to play important roles. Using a model of transient global ischemia, we have analyzed the NO system and the p53, bax and bcl-2 response in the cerebral cortex of aged rats. Although immediately after ischemia the NO level is maintained, the reperfusion period increases NO concentrations together with the following: (i) greater bulk-protein nitration mainly due to a 50-kDa immunoreactive band; (ii) an increase in p53 protein; and (iii) an up-regulation of Bax together with a down-regulation of Bcl-2. These results match up with induced endothelial nitric oxide synthase expression immediately after ischemia and in neuronal nitric oxide synthase with the reperfusion. However, inducible nitric oxide synthase was not altered with ischemia/reperfusion. Altogether, these data suggest that NO production in cerebral cortex of aged ischemic animals is due to the constitutive NO synthase isoforms. This response is accompanied by the increased expression of pro-apoptotic proteins.
Asunto(s)
Envejecimiento/metabolismo , Isquemia Encefálica/metabolismo , Corteza Cerebral/metabolismo , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico/metabolismo , Animales , Western Blotting/métodos , Modelos Animales de Enfermedad , Regulación de la Expresión Génica/fisiología , Humanos , Masculino , NADPH Deshidrogenasa/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Ratas , Proteína p53 Supresora de Tumor/metabolismo , Proteína X Asociada a bcl-2RESUMEN
This study examines the expression and cellular distribution pattern of nitric oxide synthase (NOS) isoforms, nitrotyrosine-derived complexes, and the nitric oxide (NO) production in the cerebellum of rats with cirrhosis induced by thioacetamide (TAA). The results showed local changes in the tissue distribution pattern of the NOS isoforms and nitrated proteins in the cerebellum of these animals. Particularly, eNOS immunoreactivity in perivascular glial cells of the white matter was detected only in TAA-treated animals. In addition, although neither neuronal NOS (nNOS) nor inducible NOS (iNOS) cerebellar protein levels appeared to be affected, the endothelial NOS (eNOS) isoform significantly increased its expression, and NO production slightly augmented in TAA-treated rats. These NOS/NO changes may contribute differently to the evolution of the hepatic disease either by maintaining the guanosine monophosphate-NO signal transduction pathways and the physiological cerebellar functions or by inducing oxidative stress and cell damage. This model gives rise to the hypothesis that the upregulation of the eNOS maintains the physiological production of NO, while the iNOS is silenced and the nNOS remains unchanged. The differential NOS-distribution and expression pattern may be one of the mechanisms involved to balance cerebellar NO production in order to minimize TAA toxic injury. These data help elucidate the role of the NOS/NO system in the development and progress of hepatic encephalopathy associated with TAA cirrhosis.
Asunto(s)
Cerebelo/metabolismo , Cirrosis Hepática Experimental/metabolismo , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico/metabolismo , Tirosina/análogos & derivados , Animales , Western Blotting/métodos , Modelos Animales de Enfermedad , Regulación Enzimológica de la Expresión Génica , Inmunohistoquímica/métodos , Cirrosis Hepática Experimental/inducido químicamente , Masculino , NADPH Deshidrogenasa/metabolismo , Nitratos/metabolismo , Óxido Nítrico Sintasa de Tipo I , Óxido Nítrico Sintasa de Tipo II , Óxido Nítrico Sintasa de Tipo III , Nitritos/metabolismo , Ratas , Ratas Wistar , Tioacetamida , Tirosina/metabolismo , Regulación hacia ArribaRESUMEN
Increased activities of several detoxifying and antioxidant enzymes were detected in mollusc and fish from Spanish littoral areas with high metal contents. Ethanolic extracts from molluscs contained direct-acting and polar genotoxins of oxidative type, which were detected by strain TA102 of S. typhimurium and catalase-deficient strains of E. coli. Animals from contaminated sites contained less genotoxins than those from control areas. Polluted fishes displayed highly induced cytochrome P-450 activity and increased promutagen activation capabilities. In addition, specific forms of glutathione transferase and superoxide dismutase were induced, particularly highly acidic forms.
Asunto(s)
Peces/metabolismo , Moluscos/enzimología , Oxidorreductasas/metabolismo , Agua de Mar , Contaminación Química del Agua/análisis , Animales , Sistema Enzimático del Citocromo P-450/metabolismo , Escherichia coli/efectos de los fármacos , Metales/análisis , Moluscos/efectos de los fármacos , Pruebas de Mutagenicidad , Salmonella typhimurium/efectos de los fármacos , EspañaRESUMEN
GST isonzyme patterns were studied in the cytosolic fraction of liver, kidney and lung of sheep exposed to industrial metal pollutants and compared with those of control animals. The methodology included the determination of enzymatic activities with several subunit-specific substrates (DCNB, NPB, EPNP and EA) and Western blotting using antibodies to specific rat GST subunits 1, 8 (alpha class), 3 (mu class) and 7 (pi class). In liver and lung, crossed reactivities with subunits 1 and 3 were absent in the controls but were present in exposed animals. Just the opposite result was obtained for subunit 8 crossed reactivity that was only in the control animals. In the kidney, crossed reactivities towards subunits 3 and 8 were absent and crossed reactivity equivalent to subunit 7 was present in all animals, and equivalent to subunit 1 was weakly induced in exposed animals. A 3.3-fold increase in the activity with NPB detected in the kidneys of exposed animals points to the induction of a theta class isoenzymes. Clear increases were found in the livers of exposed animals in the activities with CDNB (1.8-fold), DCNB (2.6-fold) and EPNP (2.1-fold), but no differences were found in the lungs with any of the substrates. The GST isoenzyme pattern of liver and lung could be, in principle, a useful biomarker of exposure to environmental pollution in sheep.
Asunto(s)
Contaminación Ambiental/efectos adversos , Glutatión Transferasa/metabolismo , Isoenzimas/metabolismo , Animales , Western Blotting , Dieta , Electroforesis en Gel de Poliacrilamida , Contaminantes Ambientales/toxicidad , Femenino , Glutatión Transferasa/química , Inmunohistoquímica , Isoenzimas/química , Metales/toxicidad , Ovinos , EslovaquiaRESUMEN
The comparative toxicity of a variety of oxidative stressors was studied in the epithelioma papulosum cyprini line from carp using the neutral red cytotoxicity assay. LC50's decreased in the order t-butylhydroperoxide > hydrogen peroxide > diquat > paraquat. The cytotoxicity of hydrogen peroxide was significantly reduced when the cells were grown in L-15 medium rather than MEM and this could be attributed to elevated cellular glutathione and metallothionein levels and higher activities of GSH-dependent detoxification systems. The protective effect of metallothionein in radical scavenging was demonstrated by decreased toxicity of the redox-cycling toxicants, diquat and menadione after metallothionein levels had been pre-induced by Cd-exposure. This study demonstrates the relationship between toxic effects of oxidative stressors and expression of detoxification systems in fish.
Asunto(s)
Carpas/metabolismo , Glutatión/metabolismo , Herbicidas/toxicidad , Metalotioneína/metabolismo , Estrés Oxidativo/efectos de los fármacos , Peróxidos/toxicidad , Animales , Línea Celular , Diquat/toxicidad , Interacciones Farmacológicas , Peróxido de Hidrógeno/toxicidad , Paraquat/toxicidad , terc-Butilhidroperóxido/toxicidadRESUMEN
OBJECTIVE: A review about the possible cellular and molecular mechanisms of aging and related neurodegenerative diseases. DEVELOPMENT: The mechanisms involved in neuronal decrease, connectivity losses and glial reactivity, detected both in neurodegenerative (Alzheimer's disease) and physiological aging, are analyzed from the morphological and histological point of view to provide the morphofunctional base of the cognitive and intellectual alterations characterizing the senescence process. Taken together, these data are correlated to the possible genetical aspects implied in this process, reviewing the most relevant results on senescence and cellular death obtained from yeast, fruit fly and nematodes; besides this, a brief review of the molecular biology of gerontogenes was carried out, and the possible mechanisms inducing aging and neurodegenerative processes are analyzed according to the state-of-the-art related theories. Finally, cellular, biochemical and genetical data are correlated in the signal transduction way implied in the increase of the intracellular calcium level as the starting point of cell death. CONCLUSIONS: The main process implied in the neuronal cell death responsible for aging and the related neurodegenerative diseases are started by different agents such as the lacking of neurotrophic factors, hypoxia, hypoglycemia, excitotoxicity, and oxygen and nitrogen free radicals.
Asunto(s)
Envejecimiento/fisiología , Encéfalo/fisiología , Enfermedades Neurodegenerativas/fisiopatología , Animales , Apoptosis , Encéfalo/citología , Calcio/metabolismo , Muerte Celular , Metabolismo Energético , Radicales Libres , Hemostasis , Humanos , Enfermedades Neurodegenerativas/etiología , Enfermedades Neurodegenerativas/genética , Óxido Nítrico/fisiología , Estrés Oxidativo/fisiología , Receptores de GlutamatoRESUMEN
Serum nitric oxide levels, systematically determined in 200 men and women from 18 to 65 year-old, undergo age and sex changes that strongly correlate with serological markers such as those related with cardiovascular functions and lipid profile.
Asunto(s)
Óxido Nítrico/sangre , Adolescente , Adulto , Factores de Edad , Anciano , Alanina Transaminasa/sangre , Fosfatasa Alcalina/sangre , Biomarcadores/sangre , Colesterol/sangre , Colinesterasas/sangre , Femenino , Humanos , Luminiscencia , Masculino , Persona de Mediana Edad , Factores Sexuales , Ácido Úrico/sangreRESUMEN
Nitrate, nitrite, and other nitroso compounds (NOxs) had been proposed as possible nitric oxide (NO) storage molecules. The present work examines, by means of chemiluminescence analysis, changes in NOx serum levels in rats 1 h before and 24, 48, and 72 h after exposure to acute hypobaric hypoxia (HH; barometric pressure [P(B)] 225 mmHg, oxygen partial pressure [PO2] 48 mmHg), normobaric hypoxia (NH; P(B) 716 mmHg [Jaén city], PO2 48 mmHg), hypobaric normoxia (HN; P(B) 225 mmHg, PO2 150 mmHg), and normobaric normoxia (NN; P(B) 716 mmHg, PO2 150 mmHg) the latter as a control group. Results show a decrease in NOx levels, which reached significance 24 h after exposure in HH animals, 4 h after exposure in the HN and NH groups, and persisted after 48 h of exposure in the HN group. NOx determinations were also performed in brain (cerebral cortex, hippocampus, decorticated brain [basal ganglia-brainstem] and cerebellum), liver, kidney, lung, and heart homogenates, 72 h after the experiment, to detect persistent effects when serum NOx levels had returned to basal values. Only in cerebellum (HN group) and hippocampus (HN and NH groups) were NOx levels significantly lower than in controls. We conclude that not only acute hypobaric hypoxia but also either hypobaria or hypoxia alone induce changes in NOx serum levels. Moreover, all three episodes involve a decrease in NOxs, greater and longer-lasting in hypoxia alone than in hypobaria and hypoxia together. The exhaustion of these NO-storage molecules could be critical when, as during a hypoxic episode, the L-arginine/NOS pathway is impaired.
Asunto(s)
Presión Atmosférica , Hipoxia/metabolismo , Mediciones Luminiscentes/métodos , Óxido Nítrico/metabolismo , Compuestos Nitrosos/metabolismo , Adaptación Fisiológica , Animales , Cámaras de Exposición Atmosférica , Química Encefálica , Riñón/metabolismo , Hígado/metabolismo , Pulmón/metabolismo , Masculino , Miocardio/metabolismo , Óxido Nítrico/sangre , Consumo de Oxígeno/fisiología , Ratas , Ratas Wistar , Factores de TiempoRESUMEN
3-nitrotyrosine (NO2-Tyr) is thought to be a specific marker of cell injury during oxidative damage. We have evaluated the role of poly(ADP-ribose)polymerase-1 (PARP-1) in protein nitration after treatment of immortalized fibroblasts parp-1+/+ and parp-1-/- with the alkylating agent 2'-methyl-2'-nitroso-urea (MNU). Both cell lines showed increased iNOS expression following MNU treatment in parallel with a selective induction of tyrosine nitration of different proteins. PARP-1 deficient cells displayed a delayed iNOS accumulation, reduced number of nitrated proteins, and a lower global nitrotyrosine "footprint." We have identified the mitochondrial compartment as the major site of oxidative stress during DNA damage, being MnSOD one of the NO2-Tyr-modified proteins, but not in parp-1-/- cells. These results suggest that NO-derived injury can be modulated by proteins involved in the response to genotoxic damage, such as PARP-1, and may account for the limited oxidative injury in parp-1 knockout mice during carcinogenesis and inflammation.
Asunto(s)
Daño del ADN , Poli(ADP-Ribosa) Polimerasas/metabolismo , Procesamiento Proteico-Postraduccional , Tirosina/análogos & derivados , Animales , Fibroblastos/citología , Lipopolisacáridos/farmacología , Metilnitrosourea/farmacología , Ratones , Ratones Noqueados , Microtúbulos/metabolismo , Mitocondrias/metabolismo , Mutación/genética , Células 3T3 NIH , Óxido Nítrico Sintasa de Tipo II/metabolismo , Estrés Oxidativo , Poli(ADP-Ribosa) Polimerasa-1 , Poli(ADP-Ribosa) Polimerasas/deficiencia , Poli(ADP-Ribosa) Polimerasas/genética , Superóxido Dismutasa/metabolismo , Factores de Tiempo , Tirosina/metabolismoRESUMEN
A fast, sensitive and direct method has been developed for the determination of glutathione peroxidase activity (both selenium- and non-selenium-dependent) in cell-free preparations. The assay is based on the separation and quantitation of reduced and oxidized glutathione by capillary electrophoresis. The electrophoretic separation buffer was 100 mM sodium tetraborate (pH 8.2) containing 100 mM sodium dodecylsulphate. A micellar electrokinetic mechanism took place under these conditions, and a total mass recovery was observed for both peptides. The reproducibility of migration times was excellent (less than 3% variability). A linear detector response range was observed in the range 5-50 U/ml, and both the reproducibility and accuracy were satisfied. Samples out of this linear range could be analysed by either increasing the reaction time or diluting the enzyme preparation. The results obtained with the new direct capillary electrophoresis assay were compared with those derived from a reversed phase high-performance liquid chromatographic and spectrophotometric coupled assay. A very good agreement was found between the two direct assay methods in all samples. Capillary electrophoresis is a versatile technique that allows the automation of the glutathione peroxidase assay in a reproducible manner and within a relatively short time with sufficient accuracy and precision.
Asunto(s)
Electroforesis/métodos , Glutatión Peroxidasa/análisis , Animales , Bovinos , Sistema Libre de Células , Cromatografía Líquida de Alta Presión , Eritrocitos/enzimología , Peces , Glutatión/metabolismo , Hígado/enzimología , Masculino , Oxidación-Reducción , Ratas , Espectrofotometría UltravioletaRESUMEN
The analysis of glutathione transferase (GST) isoenzyme patterns is of interest in many fields as hepatic glutathione transferase activity is increased by exposure to a variety of xenobiotics and its isoenzymatic forms are induced differentially. A high-performance liquid chromatography method has been developed for the rapid determination of individual isoenzyme levels in crude extracts using an anion-exchange column connected to an on-line system to automatically detect GST activity with 1-chloro-2,4-dinitrobenzene as the substrate. When 50-200 microliters of a cytosolic fraction of fish liver containing up to 15 mg/ml of protein and less than 2 units of GST were injected, a high resolution and highly reproducible chromatogram was obtained. The activity profile determined automatically showed eight to twelve peaks (depending on the sample) that were quantified and could be classified into three groups. Starting from intact tissue, a complete isoenzyme pattern could be obtained in less than 3 h. The method has been applied to ecotoxicological studies with fish samples.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Peces , Glutatión Transferasa/análisis , Isoenzimas/análisis , Hígado/enzimología , Animales , Dinitroclorobenceno/metabolismo , Indicadores y ReactivosRESUMEN
Fourteen isoforms of glutathione S-transferase (GST) have been separated and purified from mullet (Mugil cephalus) liver by scaling up an automatic analytical method based on anionic exchange chromatography. The activity of each isoenzyme with several substrates was determined. Dimeric combinations of six subunits make up this heterogeneous isoenzyme population. Five of these were resolved by reverse phase chromatography; four of them, named a, b, c and d, were present in more than one isoform, had the same apparent molecular mass (25.2 kDa) by SDS-PAGE, and were immunochemically related to plaice GST-A and possibly to rat GST-5 but not to plaice GST-B or any other rat GST subunit; they would belong to the theta class. Subunit e was only present in isoenzyme I which was basic, had an apparent molecular mass of 23.4 kDa and would belong to the alpha class, since it was recognized by antibodies towards plaice GST-B and rat GST-1 and GST-8 and less intensely by anti-(rat)GST-2. Another subunit, named f, with 25.2 kDa apparent molecular mass that could not be distinguished by reverse phase chromatography, was detected immunochemically by positive reaction with antibodies to rat GST-1 and GST-2 in addition to reaction with anti-(plaice)GST-A. As suggested by these results we discuss the existence of genetic polymorphism, the differential expression and the evolutionary relationships of mullet GSTs.
Asunto(s)
Glutatión Transferasa/aislamiento & purificación , Isoenzimas/aislamiento & purificación , Hígado/enzimología , Perciformes/fisiología , Animales , Cromatografía de Afinidad , Glutatión Transferasa/química , Glutatión Transferasa/inmunología , Técnicas Inmunológicas , Punto Isoeléctrico , Peso Molecular , Ratas , Especificidad de la EspecieRESUMEN
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography has played a relevant role, being reported in all the papers reviewed, whereas other preparative techniques such as electrophoresis and isoelectric focusing were less employed and have been reported in only ca. 3% of cases. Frequently, several different chromatographic modes and several rechromatography steps have been employed. The use of at least three different chromatographic modes has been reported in 53% of total reviewed papers, whereas 41% of them employed two differents modes and in only 6% a single preparative chromatographic step was used. To evaluate losses and improve recovery, analytical methods for quantitation of protein and assay of enzymatic activity must be used in each purification step. Among these analytical techniques, gel electrophoresis, under denaturing conditions, has been widely used to assess purity of enzyme preparation. A discussion of the different activity assay methods used for these three enzymes is also presented in this article.
Asunto(s)
Cromatografía Liquida/métodos , Electroforesis en Gel de Poliacrilamida/métodos , Glutatión Peroxidasa/aislamiento & purificación , Glutatión Reductasa/aislamiento & purificación , Glutatión Transferasa/aislamiento & purificación , Glutatión Peroxidasa/análisis , Glutatión Reductasa/análisis , Glutatión Transferasa/análisisRESUMEN
We have found conclusive evidence for inducible nitric oxide synthase (iNOS) activity in rainbow trout (Oncorhynchus mykiss) tissue by means of biochemical, immunohistochemical, and immunoblotting analyses. This Ca(2+)-independent enzyme uses L-arginine to produce nitric oxide and L-citrulline. It was significantly inhibited by the L-arginine analogs N(omega)-monomethyl-L-arginine and N(G)-nitro-L-arginine methyl ester. Kinetic analyses showed typical Michaelian behavior with no evidence of cooperative effects. The specific activities of the liver and head kidney enzymes were 27 and 106 pmoles. min(-1). mg protein(-1), respectively, with similar values for K(m) (11 microM), all of which correspond well with the values for other previously characterized iNOS. Western blot analyses revealed a single band of M(R) = 130 kDa tested with an iNOS antiserum. At the ultrastructural level, cells with NADPH-diaphorase activity and iNOS immunoreactivity were identified as being heterophilic granulocytes in head kidney tissue and neutrophils and macrophages in hepatic tissue. The presence of an iNOS isoform in these fish tissues implies that these cells are capable of generating nitric oxide, thus pointing to the potential role of this enzyme in fish defense mechanisms.
Asunto(s)
Óxido Nítrico Sintasa/metabolismo , Oncorhynchus mykiss/metabolismo , Animales , Western Blotting , Granulocitos/enzimología , Inmunohistoquímica , Riñón/citología , Riñón/enzimología , Cinética , Hígado/citología , Hígado/enzimología , Macrófagos/enzimología , NADPH Deshidrogenasa/metabolismo , Neutrófilos/enzimología , Óxido Nítrico Sintasa de Tipo II , Distribución TisularRESUMEN
The activity of several antioxidant and detoxifying enzymes, superoxide dismutase SOD, GSH peroxidase GSHPx, GSSG reductase GSR and GSH S transferase GST, the contents of thiobarbituric acid reactive substances TBARS, and the SOD and GST isoenzyme patterns were studied in the livers of chubs Leuciscus cephalus from reference river areas and polluted urban sites. Livers of polluted fish contained higher concentrations of transition metals, especially copper and iron. Total GSHPx activity was 1.8 fold higher in the polluted fish than in reference animals, while the SOD and GSR activities and the TBARS content were not significantly changed. Three new SOD isoforms pI 4.45, 5.1, 5.2 and a higher intensity of the band pI 4.2 were observed after isoelectrofocusing of polluted fish extracts. Total GST activity was higher in fish from polluted areas. The GST isoenzyme pattern was studied using subunit specific substrates DCNB, EPNP, EA, NPB, NBC and by Western blot using antibodies specific to rat GST subunits 1, 8 Alpha class, 3 Mu class and 7 Pi class. Reference and polluted fish lacked cross reactivity towards Alpha class GSTs. Reference fish displayed weaker cross reactivity towards CST 7 and 2.3 fold lower activity with EA, while higher cross reaction with GST 3 was observed in polluted fish.
Asunto(s)
Peces/metabolismo , Glutatión Transferasa/metabolismo , Isoenzimas/metabolismo , Hígado/enzimología , Contaminación del Agua , Animales , Cromatografía Líquida de Alta Presión , Citosol/enzimología , Glutatión Transferasa/aislamiento & purificación , Isoenzimas/aislamiento & purificación , Valores de Referencia , Agua de Mar , EspañaRESUMEN
Objetivo. Se lleva a cabo una revisión actualizada sobre las posibles causas celulares y moleculares que subyacen al proceso de envejecimiento cerebral así como a las enfermedades neurodegenerativas asociadas. Desarrollo. A partir de las lesiones morfológicas e histológicas que se detectan tanto en procesos neurodegenerativos como durante el envejecimiento fisiológico, se analizan las causas que conducen a la pérdida de neuronas y de conectividad nerviosa, así como a los procesos de reactividad glial, como base morfofuncional del deterioro de los procesos cognitivo e intelectual que caracterizan la senescencia. Se correlacionan todos estos datos con las posibles bases genéticas del proceso de envejecimiento, llevando a cabo una revisión de los hallazgos más relevantes sobre senescencia y muerte celular obtenidos en levaduras, mosca de la fruta y nematodos. Tras una breve revisión sobre gerontogenes y mecanismos de apoptosis, se analizan las causas inductoras del proceso de envejecimiento y neurodegeneración, y se interrelacionan las diferentes hipótesis que barajan las teorías más actuales sobre el tema. Finalmente se integran todos los datos celulares, bioquímicos y genéticos, a partir de la puesta en marcha de sistemas de transducción de señales que provocan la elevación de los niveles de calcio citosólico y el disparo del proceso de muerte celular. Conclusiones. Diversas causas inductoras, que pueden activar o inhibir grupos de genes, entre las que se cuentan, deficiencias de factores neurotróficos, hipoxia e hipoglucemia, excitotoxicidad, producción de radicales libres de oxígeno y de nitrógeno, desencadenan procesos de muerte neuronal responsables del envejecimiento y de las enfermedades neurodegenerativas asociadas (AU)