Removal of bacteria, protozoa and viruses through a multiple-barrier household water disinfection system.

Municipal water disinfection systems in some areas are not always able to meet water consumer needs, such as ensuring distributed water quality, because household water management can be a contributing factor in water re-contamination. This fact is related to the storage options that are common in places where water is scarce or is distributed over limited time periods. The aim of this study is to assess the removal capacity of a multiple-barrier water disinfection device for protozoa, bacteria, and viruses. Water samples were taken from households in Mexico City and spiked with a known amount of protozoa (Giardia cyst, Cryptosporidium oocyst), bacteria (Escherichia coli), and viruses (rotavirus, adenovirus, F-specific ribonucleic acid (FRNA) coliphage). Each inoculated sample was processed through a multiple-barrier device. The efficiency of the multiple-barrier device to remove E. coli was close to 100%, and more than 87% of Cryptosporidium oocysts and more than 98% of Giardia cysts were removed. Close to 100% of coliphages were removed, 99.6% of the adenovirus was removed, and the rotavirus was almost totally removed. An effect of site by zone was detected; this observation is important because the water characteristics could indicate the efficiency of the multiple-barrier disinfection device.

Potentially pathogenic nontuberculous mycobacteria found in aquatic systems. Analysis from a reclaimed water and water distribution system in Mexico City.

We investigated the presence of nontuberculous mycobacteria (NTM) in three Mexican aquatic systems to evaluate the prevalence with the distribution of NTM species. Key physicochemical parameters of the water samples were determined to find correlations with the species' distributions. We used multilocus sequence analysis (MLSA) based on hsp65, rpoB, and 16S rRNA fragments to determine their taxonomic affiliations. NTM were recovered from water distribution systems and reclaimed water from the Mexico City Metropolitan Area (MCMA). The isolated species were associated with a temperature of 21°C and pH >7.7. The phylogenetic analysis showed that eight of the 14 different NTM strains were unambiguously classifiable: Mycobacterium peregrinum, M. nonchromogenicum (2), M. smegmatis (2), M. fortuitum, M. avium ssp. hominissuis, M. arupense, M. gordonae, and M. chitae. One strain was tentatively identified as M. mantenni/ scrofulaceum and another strain was related to M. porcinum/M. septicum. All NTM species identified in the water distribution system were also detected in the reclaimed water, but some species from the reclaimed water were not found in the water distribution systems. Two of the identified species found in the reclaimed water, M. avium and M. fortuitum, are considered important human opportunistic pathogens.

Helicobacter pylori and other enteric bacteria in freshwater environments in Mexico City.

BACKGROUND: Helicobacter pylori infection is common in the Mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear. METHODS: H. pylori was detected by polymerase chain reaction (PCR) technique in three aquatic systems located in the Mexico City area. In addition, microbiologic cultures and physicochemical parameters were measured. The systems were sampled over an 18-month period (1997-1999), resulting in a total of 212 samples for the different analyses. RESULTS: Twenty-one percent of the samples (16/77) were positive for H. pylori; of these, 42% (5/12) were confirmed for cagA gene detection by PCR hybridization. Microbiologic samples (n = 74) yielded Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii, and Vibrio fluvialis. In the samples for physicochemical analyses (n = 61), low concentrations of dissolved oxygen were detected and residual chlorine was less than the inactivation dose, both providing conditions for potential survival of H. pylori and other enteric pathogens in these environments. CONCLUSIONS: The results of this study suggest that, in Mexico City, water used for human consumption and irrigation may play an important role as a vehicle in the transmission of H. pylori as well as infection by other known enteric pathogens.

Are megacities viable? A cautionary tale from Mexico City.

PIP: This article describes the poor environmental and living conditions in Mexico City due to its huge size. Mexico City's size is a challenge to sustainability, and the outcome is unknown. Mexico City and the geographic basin surrounding it included about 18.5 million population in 1995. The basin and surrounding volcanic ranges include nine major environmental zones. Urban growth followed four stages. Different cultures applied different solutions to water supply problems. The basin shifted from self-sufficiency to reliance on 31% of supplies from external watersheds. The water table is declining and canals are polluted. Irrigated agriculture is disappearing. There is an average water deficit of over 800 million cubic meters per year. Mexico City is actually sinking due to groundwater exploitation. There is bacterial contamination of wells due to improper seals. About 75% of the population has access to wastewater treatment and sanitation, but sewage treatment plants operate at under 50% efficiency and treat only about 7% of the total wastewater. Atmospheric pollution from suspended particles has been a problem for decades. Ozone was the most significant air contaminant in 1994. Lead was the most harmful pollutant in 1986. Air pollutants may be the source of submucosal inflammations. Industrial areas are contaminated with suspended particles and sulfur dioxide. High traffic areas have high carbon monoxide levels. Atmospheric pollution has affected the quality of the rainwater. The city survives by importing food, energy, wood, water, building materials, and other products. The development model aims to improve quality of life. The city has been the center of political power since Aztec times, and its preeminent position forces government action. The author concludes that there are limits to urbanization, which the city is approaching rapidly.^ieng

Helicobacter pylori in water systems for human use in Mexico City.

Helicobacter pylori infection is associated with peptic ulcers and gastric cancer in humans. Transmission of H. pylori is still not certain with some epidemiological data suggesting water as a possible transmission route. The objective of this study was to detect H. pylori 16S rRNA gene in five water systems in the Mexico City area. Samples were taken between 1997 and 2000 from extraction wells (system 1), from dams used as water sources, both pre- and post-treatment (systems 2 and 3), treated wastewater (system 4) and non-treated wastewater (system 5). Detection of the H. pylori 16S rRNA gene in water samples was carried out using nested PCR in 139 water samples and confirmed by using cagA gene detection by PCR-hybridisation. The results showed the presence of H. pylori in 58 (42%) of the water samples in total with a prevalence of 68% in system 1, 100% in system 2, 0% in system 3, 17% in system 4 and 20% in system 5. This first stage showed the presence of H. pylori in the tested water systems; nevertheless, viability of the microorganism in water and vegetables needs to be confirmed as well as demonstration of a relationship between human and environmental strains.