RESUMEN
We demonstrated the possibility of penetration of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen into mouse macrophages. It was found that after 3-h incubation with nanoparticles in a concentration of 15 mg/liter at 37oC, they were seen in only 13% macrophages. In about 90% cells, the nanoparticles were detected within the cytoplasm. Under these conditions, membrane damage was revealed in 25% cells. These results should be taken into account in further development and application of nanomaterials for diagnostic and therapeutic purposes in oncology.
Asunto(s)
Antígenos de Superficie/metabolismo , Muerte Celular/efectos de los fármacos , Medios de Contraste/toxicidad , Glutamato Carboxipeptidasa II/metabolismo , Macrófagos Peritoneales/efectos de los fármacos , Nanopartículas de Magnetita/toxicidad , Animales , Antígenos de Superficie/química , Carbocianinas/química , Línea Celular Tumoral , Medios de Contraste/química , Endocitosis/fisiología , Colorantes Fluorescentes/química , Glutamato Carboxipeptidasa II/química , Oro/química , Humanos , Macrófagos Peritoneales/metabolismo , Macrófagos Peritoneales/ultraestructura , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/ultraestructura , Masculino , Ratones , Cultivo Primario de Células , Próstata/diagnóstico por imagen , Próstata/metabolismo , Próstata/patología , Unión ProteicaRESUMEN
Magnetic resonance imaging (MRI) is one of the most perspective methods of noninvasive visualization in medicine, and use of contrast agents significantly its potentialities extends. Iron oxide nanoparticles are promising contrast agents, but in fact all the data on their efficiency were obtained in high-field tomographs for experimental animals. We studied the possibility of using magnetic nanoparticles for MRI visualization of rat brain glioblastoma at the most common clinical field 1.5 T The data indicate the efficiency of iron oxide magnetic nanoparticles as contrast agents for 1.5 T MR tomographs.
Asunto(s)
Medios de Contraste/química , Compuestos Férricos/química , Glioblastoma/diagnóstico por imagen , Imagen por Resonancia Magnética/métodos , Nanopartículas de Magnetita/química , Animales , Encéfalo/diagnóstico por imagen , Línea Celular Tumoral , RatasRESUMEN
We studied dependences of T2 relaxation time on magnetic field and concentration of nanoparticles. It was found that nanocontrast media are effective under the influence of the magnetic fields in the range 0.3-7 T. Data of electron paramagnetic resonance confirm the assumption on aggregation of nanoparticles not coated with proteins in high magnetic fields.
Asunto(s)
Medios de Contraste/química , Compuestos Férricos/química , Campos Magnéticos , Imagen por Resonancia Magnética/métodos , Nanopartículas/químicaRESUMEN
We synthesized a fluorescence conjugate and modified magnetite-gold nanoparticles carrying prostate specific membrane antigen (PSMA) as the ligand. Analysis of their binding to human prostate cancer cell lines PC-3 (PSMA-) and LNCaP (PSMA+) showed selective interaction of the synthesized conjugate and modified nanoparticles with LNCaP cells. These findings suggest that these nanoparticles can be used in tissue-specific magnetic-resonance imaging.