RESUMEN
Age-related cognitive decline, a common component of the brain aging process, is associated with significant impairment in daily functioning and quality of life among geriatric adults. While the complexity of mechanisms underlying cognitive aging are still being elucidated, microbial exposure and the multifactorial inflammatory cascades associated with systemic infections are emerging as potential drivers of neurological senescence. The negative cognitive and neurobiological consequences of a single pathogen-associated inflammatory experience, such as that modeled through treatment with lipopolysaccharide (LPS), are well documented. Yet, the brain aging impacts of repeated, intermittent inflammatory challenges are less well studied. To extend the emerging literature assessing the impact of infection burden on cognitive function among normally aging mice, here, we repeatedly exposed adult mice to intermittent LPS challenges during the aging period. Male 10-month-old C57BL6 mice were systemically administered escalating doses of LPS once every two weeks for 2.5 months. We evaluated cognitive consequences using the non-spatial step-through inhibitory avoidance task, and both spatial working and reference memory versions of the Morris water maze. We also probed several potential mechanisms, including cortical and hippocampal cytokine/chemokine gene expression, as well as hippocampal neuronal function via extracellular field potential recordings. Though there was limited evidence for an ongoing inflammatory state in cortex and hippocampus, we observed impaired learning and memory and a disruption of hippocampal long-term potentiation. These data suggest that a history of intermittent exposure to LPS-induced inflammation is associated with subtle but significantly impaired cognition among normally aging mice. The broader impact of these findings may have important implications for standard of care involving infections in aging individuals or populations at-risk for dementia.
Asunto(s)
Lipopolisacáridos , Potenciación a Largo Plazo , Ratones , Animales , Masculino , Lipopolisacáridos/farmacología , Lipopolisacáridos/metabolismo , Calidad de Vida , Ratones Endogámicos C57BL , Cognición/fisiología , Envejecimiento/metabolismo , Inflamación/complicaciones , Hipocampo/metabolismo , Aprendizaje por LaberintoRESUMEN
Neuronal Ceroid Lipofuscinosis (NCL), also known as Batten disease, is an incurable childhood brain disease. The thirteen forms of NCL are caused by mutations in thirteen CLN genes. Mutations in one CLN gene, CLN5, cause variant late-infantile NCL, with an age of onset between 4 and 7 years. The CLN5 protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. Mutations in CLN5 are associated with the accumulation of autofluorescent storage material in lysosomes, the recycling units of the cell, in the brain and peripheral tissues. CLN5 resides in the lysosome and its function is still elusive. Initial studies suggested CLN5 was a transmembrane protein, which was later revealed to be processed into a soluble form. Multiple glycosylation sites have been reported, which may dictate its localisation and function. CLN5 interacts with several CLN proteins, and other lysosomal proteins, making it an important candidate to understand lysosomal biology. The existing knowledge on CLN5 biology stems from studies using several model organisms, including mice, sheep, cattle, dogs, social amoeba and cell cultures. Each model organism has its advantages and limitations, making it crucial to adopt a combinatorial approach, using both human cells and model organisms, to understand CLN5 pathologies and design drug therapies. In this comprehensive review, we have summarised and critiqued existing literature on CLN5 and have discussed the missing pieces of the puzzle that need to be addressed to develop an efficient therapy for CLN5 Batten disease.
Asunto(s)
Proteínas de Membrana de los Lisosomas/genética , Lisosomas/metabolismo , Mutación , Lipofuscinosis Ceroideas Neuronales/patología , Animales , Humanos , Proteínas de Membrana de los Lisosomas/metabolismo , Lipofuscinosis Ceroideas Neuronales/etiología , Lipofuscinosis Ceroideas Neuronales/metabolismoRESUMEN
Plasmids pUB110 and pBC16 were introduced by protoplast transformation into the entomocidal bacterium Bacillus sphaericus 1593. Transformants expressed the antibiotic resistance determinants present on the plasmid and exhibited sporulation frequencies and larvicidal toxicities which were equivalent to those characteristic of the parent strain. These transformations constitute the first report of genetic transfer in B. sphaericus.
Asunto(s)
Bacillus/genética , Plásmidos , Transformación Bacteriana , Bacillus/efectos de los fármacos , Bacillus subtilis/efectos de los fármacos , Bacillus subtilis/genética , Enzimas de Restricción del ADN , ADN Bacteriano , Farmacorresistencia Microbiana , Neomicina/farmacología , Protoplastos , Tetraciclinas/farmacologíaRESUMEN
A recombinant plasmid was constructed by ligation of chromosomal DNA from a sulfanilamide-resistant strain of Bacillus subtilis to the plasmid vector pUB110 which specifies neomycin resistance. Recombinant molecules generated in vitro were introduced into a B. subtilis recipient strain which carried the recE4 mutation, and selection was for neomycin-sulfanilamide-resistant transformants. A single colony was isolated containing the recombinant plasmid pKO101. This 6.3-megadalton plasmid simultaneously conferred resistance to neomycin and sulfanilamide when transferred into sensitive Rec+ or Rec- cells by either transduction or transformation.
Asunto(s)
Bacillus subtilis/genética , Clonación Molecular , Genes Bacterianos , Sulfanilamidas/farmacología , ADN Recombinante , Farmacorresistencia Microbiana , Neomicina , Plásmidos , Transformación BacterianaRESUMEN
UV light from a germicidal lamp rapidly reduced the viability of Bacillus sphaericus 1593 spores, but insecticidal activity was resistant to inactivation by continuous exposure to UV light for 4 h.