RESUMEN
A herpesvirus has been isolated from spontaneously degenerating cultures of cervical tumor cells grown in vitro. The virus was identified as a type 2 herpesvirus on the basis of biologic properties, including plaque morphology and microtubule formation in infected HEp-2 cells, and of immunologic specificity as determined by neutralization. Herpesvirus antigens and virus particles were not seen in duplicate cultures of viable cervical tumor cells.
Asunto(s)
Carcinoma/microbiología , Herpesviridae/aislamiento & purificación , Neoplasias del Cuello Uterino/microbiología , Antígenos Virales/análisis , Carcinoma/patología , Células Cultivadas , Femenino , Herpesviridae/inmunología , Humanos , Microscopía Electrónica , Pruebas de Neutralización , Neoplasias del Cuello Uterino/patología , Cultivo de VirusRESUMEN
Our study demonstrates for the first time that Herpesvirus saimiri can induce acute lymphocytic leukemia in owl monkeys (Aotus trivirgatus) and that malignant lymphoma can be induced in this species of nonhuman primates by the inoculation of the virus by various routes (intravenous, sub-cutaneous, and intradermal).
Asunto(s)
Animales , Modelos Animales de EnfermedadRESUMEN
Twenty-two of 39 rabbits inoculated with Herpesvirus saimiri developed malignant lymphoma and either died or were killed between 17 and 165 days after inoculation. No clinical signs were present in animals developing the disease before 46 days, but all other rabbits had a severe conjunctivitis, nasal discharge, and dyspnea resulting from a lymphocytic invasion of the ocular and nasal tissues. Four rabbits developed terminal leukemia. Pathologically, the disease resembled H. saimiri malignant lymphoma in nonhuman primates; there was extensive diffuse infiltration of most organs and tissues with either a lymphocytic or lymphoblastic infiltrate. Tumor nodules or masses seen in some forms of malignant lymphoma were not present. In contrast to nonhuman primates, all affected rabbits showed invasion of the skin of the nose and eyelids, conjunctiva, iris, ciliary body, and choroid. In 3 rabbits there was slight infiltration into the brain, not noted in nonhuman primates. The susceptibility of rabbits extended the host range of H. saimiri beyond the order Primates.
Asunto(s)
Infecciones por Herpesviridae , Herpesviridae , Herpesvirus Saimiriino 2 , Linfoma/etiología , Conejos , Animales , Recuento de Células Sanguíneas , Conjuntivitis/etiología , Conjuntivitis/patología , Leucemia Experimental/etiología , Pulmón/patología , Linfoma/sangre , Linfoma/patología , Mucosa Nasal/patología , Neoplasias Experimentales/sangre , Neoplasias Experimentales/etiología , Neoplasias Experimentales/patología , Infecciones del Sistema Respiratorio/etiología , Infecciones del Sistema Respiratorio/patología , Factores de TiempoRESUMEN
The Veterinary Biologics Information System is a project the main goal of which is to collect, analyze and manage data concerning registered veterinary biologics and biotechnological products employed to control animal diseases by the countries of the Americas. Data from each country was gathered by cataloguing each biological product on a questionnaire form. The collected data was then fed into an electronic data base system. A retrieval program was subsequently developed, allowing the user quick access to all data stored in the system. The system operates in English and Spanish and includes such information as the product name, registration number, ingredients, species for which the product is approved for use, the disease against which the product is used, names of manufacturers and distributors and other distinguishing characteristics of the product including expiry dates and withdrawal times. This computerized system, with on-line data bases which can be quickly updated, provides a dynamic answer to the question of timely information concerning veterinary biological products.