Phylogenomics recovers multiple origins of portable case making in caddisflies (Insecta: Trichoptera), nature's underwater architects.

Caddisflies (Trichoptera) are among the most diverse groups of freshwater animals with more than 16 000 described species. They play a fundamental role in freshwater ecology and environmental engineering in streams, rivers and lakes. Because of this, they are frequently used as indicator organisms in biomonitoring programmes. Despite their importance, key questions concerning the evolutionary history of caddisflies, such as the timing and origin of larval case making, remain unanswered owing to the lack of a well-resolved phylogeny. Here, we estimated a phylogenetic tree using a combination of transcriptomes and targeted enrichment data for 207 species, representing 48 of 52 extant families and 174 genera. We calibrated and dated the tree with 33 carefully selected fossils. The first caddisflies originated approximately 295 million years ago in the Permian, and major suborders began to diversify in the Triassic. Furthermore, we show that portable case making evolved in three separate lineages, and shifts in diversification occurred in concert with key evolutionary innovations beyond case making.

Burden of disease from Helicobacter pylori infection in western Canadian Arctic communities.

BACKGROUND: Indigenous communities across the circumpolar north have elevated H. pylori (Hp) prevalence and stomach cancer incidence. We aimed to describe the Hp-associated disease burden among western Canadian Arctic participants in community-driven projects that address concerns about health risks from Hp infection. METHODS: During 2008-2013, participants underwent Hp screening by urea breath test and gastroscopy with gastric biopsies. We estimated Hp prevalence and prevalence by Hp status of endoscopic and histopathologic diagnoses. RESULTS: Among 878 participants with Hp status data, Hp prevalence was: 62% overall; 66% in 740 Indigenous participants; 22% in 77 non-Indigenous participants (61 participants did not disclose ethnicity); 45% at 0-14 years old, 69% at 15-34 years old, and 61% at 35-96 years old. Among 309 participants examined endoscopically, visible mucosal lesions were more frequent in the stomach than the duodenum: the gastric to duodenal ratio was 2 for inflammation, 8 for erosions, and 3 for ulcers. Pathological examination in 308 participants with gastric biopsies revealed normal gastric mucosa in 1 of 224 Hp-positive participants and 77% (65/84) of Hp-negative participants with sharp contrasts in the prevalence of abnormalities between Hp-positive and Hp-negative participants, respectively: moderate-severe active gastritis in 50 and 0%; moderate-severe chronic gastritis in 91 and 1%; atrophic gastritis in 43 and 0%; intestinal metaplasia in 17 and 5%. CONCLUSIONS: The observed pattern of disease is consistent with increased risk of stomach cancer and reflects substantial inequity in the Hp-associated disease burden in western Arctic Canadian hamlets relative to most North American settings. This research adds to evidence that demonstrates the need for interventions aimed at reducing health risks from Hp infection in Indigenous Arctic communities.

MHz frame rate hard X-ray phase-contrast imaging using synchrotron radiation.

Third generation synchrotron light sources offer high photon flux, partial spatial coherence, and ~10-10 s pulse widths. These enable hard X-ray phase-contrast imaging (XPCI) with single-bunch temporal resolutions. In this work, we exploited the MHz repetition rates of synchrotron X-ray pulses combined with indirect X-ray detection to demonstrate the potential of XPCI with millions of frames per second multiple-frame recording. This allows for the visualization of aperiodic or stochastic transient processes which are impossible to be realized using single-shot or stroboscopic XPCI. We present observations of various phenomena, such as crack tip propagation in glass, shock wave propagation in water and explosion during electric arc ignition, which evolve in the order of km/s (µm/ns).

ID16B: a hard X-ray nanoprobe beamline at the ESRF for nano-analysis.

Within the framework of the ESRF Phase I Upgrade Programme, a new state-of-the-art synchrotron beamline ID16B has been recently developed for hard X-ray nano-analysis. The construction of ID16B was driven by research areas with major scientific and societal impact such as nanotechnology, earth and environmental sciences, and bio-medical research. Based on a canted undulator source, this long beamline provides hard X-ray nanobeams optimized mainly for spectroscopic applications, including the combination of X-ray fluorescence, X-ray diffraction, X-ray excited optical luminescence, X-ray absorption spectroscopy and 2D/3D X-ray imaging techniques. Its end-station re-uses part of the apparatus of the earlier ID22 beamline, while improving and enlarging the spectroscopic capabilities: for example, the experimental arrangement offers improved lateral spatial resolution (∼50 nm), a larger and more flexible capability for in situ experiments, and monochromatic nanobeams tunable over a wider energy range which now includes the hard X-ray regime (5-70 keV). This paper describes the characteristics of this new facility, short-term technical developments and the first scientific results.

Large-acceptance diamond planar refractive lenses manufactured by laser cutting.

For the first time, single-crystal diamond planar refractive lenses have been fabricated by laser micromachining in 300 µm-thick diamond plates which were grown by chemical vapour deposition. Linear lenses with apertures up to 1 mm and parabola apex radii up to 500 µm were manufactured and tested at the ESRF ID06 beamline. The large acceptance of these lenses allows them to be used as beam-conditioning elements. Owing to the unsurpassed thermal properties of single-crystal diamond, these lenses should be suitable to withstand the extreme flux densities expected at the planned fourth-generation X-ray sources.

Ultra-thin optical grade scCVD diamond as X-ray beam position monitor.

Results of measurements made at the SIRIUS beamline of the SOLEIL synchrotron for a new X-ray beam position monitor based on a super-thin single crystal of diamond grown by chemical vapor deposition (CVD) are presented. This detector is a quadrant electrode design processed on a 3 µm-thick membrane obtained by argon-oxygen plasma etching the central area of a CVD-grown diamond plate of 60 µm thickness. The membrane transmits more than 50% of the incident 1.3 keV energy X-ray beam. The diamond plate was of moderate purity (∼1 p.p.m. nitrogen), but the X-ray beam induced current (XBIC) measurements nevertheless showed a photo-charge collection efficiency approaching 100% for an electric field of 2 V µm(-1), corresponding to an applied bias voltage of only 6 V. XBIC mapping of the membrane showed an inhomogeneity of more than 10% across the membrane, corresponding to the measured variation in the thickness of the diamond plate before the plasma etching process. The measured XBIC signal-to-dark-current ratio of the device was greater than 10(5), and the X-ray beam position resolution of the device was better than a micrometer for a 1 kHz sampling rate.

The genus Palaeagapetus Ulmer (Trichoptera, Hydroptilidae, Ptilocolepinae) in North America.

The genus Palaeagapetus Ulmer (Trichoptera, Hydroptilidae, Ptilocolepinae) is revised in North America. Descriptions of the western species, P. nearcticus Banks 1938, are provided with the first descriptions of the female, pupa, larva, egg and case and with notes on food, habitat and annual life cycle. The male and female of the eastern species, P. celsus Ross 1936, are described or redescribed with some ecological notes. Distributions of the two species are summarized.

Calamoceras sp. nov. (Trichoptera: Calamoceratidae) from the Western Rif area, Morocco.

We describe a new species of the genus Calamoceras which currently has only two species: Calamoceras illiesi Malicky & Kumanski 1974 and Calamoceras marsupus Brauer 1865. We also discuss its ecology by analyzing some biophysical parameters. The larva and the adult of this new species, Calamoceras riffensis sp. nov. have been found by specialists from the Rif region in northwestern Morocco since 1998. All the specimens collected differ from the two known species by conspicuous morphological differences.

Pseudomonas fluorescens strain CL145A - a biopesticide for the control of zebra and quagga mussels (Bivalvia: Dreissenidae).

Zebra mussels (Dreissena polymorpha) and quagga mussels (Dreissena rostriformis bugensis) are the "poster children" of high-impact aquatic invasive species. In an effort to develop an effective and environmentally acceptable method to control their fouling of raw-water conduits, we have investigated the potential use of bacteria and their natural metabolic products as selective biological control agents. An outcome of this effort was the discovery of Pseudomonas fluorescens strain CL145A - an environmental isolate that kills these dreissenid mussels by intoxication (i.e., not infection). In the present paper, we use molecular methods to reconfirm that CL145A is a strain of the species P. fluorescens, and provide a phylogenetic analysis of the strain in relation to other Pseudomonas spp. We also provide evidence that the natural product lethal to dreissenids is associated with the cell wall of P. fluorescens CL145A, is a heat-labile secondary metabolite, and has degradable toxicity within 24 h when applied to water. CL145A appears to be an unusual strain of P. fluorescens since it was the only one among the ten strains tested to cause high mussel mortality. Pipe trials conducted under once-through conditions indicated: (1) P. fluorescens CL145A cells were efficacious against both zebra and quagga mussels, with high mortalities achieved against both species, and (2) as long as the total quantity of bacterial cells applied during the entire treatment period was the same, similar mussel mortality could be achieved in treatments lasting 1.5-12.0 h, with longer treatment durations achieving lower mortalities. The efficacy data presented herein, in combination with prior demonstration of its low risk of non-target impact, indicate that P. fluorescens CL145A cells have significant promise as an effective and environmentally safe control agent against these invasive mussels.

Five new species of the genus Cheumatopsyche (Trichoptera: Hydropsychidae) from the Phetchabun Mountains, Thailand.

Five new species of the genus Cheumatopsyche (Trichoptera: Hydropsychidae) from the Phetchabun Mountains, Thailand, are described and illustrated. The national parks and wildlife sanctuaries in the Phetchabun Mountains are recognized as areas with a high density of endemic species deserving protection. Four new species of Cheumatopsyche (C. recta, C. diversa, C. triangula, and C. tongto) have been found in Phu Khieo Wildlife Sanctuary, Phu Kradueng National Park and Thung Salaeng Luang National Park; a fifth new species (C. cava) has been found in Phu Kradueng National Park. Describing hydropsychid species is important not only to study diversity and distribution but also to facilitate eventual descriptions of larvae for use in freshwater biomonitoring programs to detect pollution.

Caddisflies (Trichoptera) of Mongolia: an updated checklist with faunistic and biogeographical notes.

To establish the biogeographic affinities of the caddisfly fauna of Mongolia, published records and results of our faunistic studies were analyzed. This study captured more than 47,000 adults collected from 386 locations beside lakes, ponds, streams/rivers, and springs in ten sub-basins of Mongolia using Malaise traps, aerial sweeping, and ultraviolet lights. In total, 201 species have been recorded, and approximately 269 species may occur in Mongolia according to our estimation. In a comparison of species richness for the family level, the Limnephilidae and Leptoceridae were the richest in species. The families Brachycentridae, Glossosomatidae, and Psychomyiidae had low species richness, but they included the most dominant species in terms of abundance and/or the percentage of occurrence in the samples from multiple sub-basins. Comparing the sub-basins, the Selenge had the highest Shannon diversity (H' = 3.3) and the Gobi sub-basin had the lowest (H' = 1.5). According to the Jaccard index of similarity, caddisfly species assemblages of Mongolia's ten sub-basins were divided into two main groups: One group includes the Selenge, Shishkhed, Bulgan, Tes, and Depression of Great Lakes sub-basins; the other group includes the Kherlen, Onon, Khalkh Gol, Valley of Lakes, and Gobi sub-basins. The majority of Mongolian species were composed of East Palearctic taxa, with a small percentage of West Palearctic and Nearctic representatives and an even smaller percentage from the Oriental region, suggesting that the Mongolian Gobi Desert is, and has been, a significant barrier to the distribution of caddisfly species between China and Mongolia.

Associating life stages and sexes of Nearctic Polycentropus Curtis, 1835 (Trichoptera: Polycentropodidae) using mitochondrial DNA barcoding.

Alpha taxonomy of caddisflies (order Trichoptera) is based primarily on male genital morphology. As such, associations of adult females and other life stages typically require conclusive association with the species' identifiable male. The aim of this study was to use molecular methods to associate females and larvae of Polycentropus species represented in the Nearctic. Analysis of mtCOI sequences using distance- and tree-based methods resulted in the association of larvae for 14 species of Polycentropus (P. alabamensis Hamilton, Harris & Lago, 1990, P. blicklei Ross & Yamamoto 1965, P. carlsoni Morse 1971, P. carolinensis Banks 1905, P. colei Ross 1941, P. confusus Hagen 1861, P. denningi Smith 1962, P. elarus Ross 1944, P. gertschi Denning 1950, Polycentropus halidus Milne 1936, P. maculatus Banks 1908, P. pentus Ross 1941, P. rickeri Yamamoto 1966, and P. variegatus Banks 1900) and females for 2 species (P. carolinensis and P. chelatus Ross & Yamamoto 1965). Searches for, and descriptions of, diagnostic morphological characters for these previously unidentifiable life forms are now possible. The identity of the larva of P. centralis Banks, 1914 is confirmed and some interesting phylogenetic relationships and a possible cryptic species and potential synonyms are implied in the results. Targets for future immature- and female-male associations are discussed along with a preliminary assessment of morphological differences among larvae.

Distribution of Ecnomus McLachlan, 1864 (Trichoptera, Ecnomidae) from the Lower Mekong River with a description of Ecnomusstungtrengensis new species.

Background: Trichoptera were surveyed from four different physiographic regions of the Lower Mekong River, including the Northern Highlands, the Khorat Plateau, the Tonle Sap Basin and the Mekong Delta in Myanmar, Laos, Thailand, Cambodia and Vietnam. New information: Twenty-three species of the genus Ecnomus, including a new species, were identified and mapped. Ecnomusmammus Malicky & Chantaramongkol, 1993 is a common species which is found from Tonle Sab Basin and Mekong Delta. Ecnomusalkmene Malicky & Chantaramongkol, 1997, E.volovicus Malicky & Chantaramongkol, 1993 and Ecnomusquordaio Malicky, 1993 are the common species in the area of the Northern Highlands and the Khorat Plateau. Ecnomusplotin Malicky & Laudee 2010 is found in the Mekong Delta. Ecnomustriangularis Sun, 1997 is a new species record for Southeast Asia. In addition, E.stungtrengensis sp. n. is described and illustrated. The male genitalia of E.stungtrengensis sp. n. are clearly different from those of other species in the genus Ecnomus by the shape of superior appendages which are slender and bent downwards distally in lateral view.

Larval Taxonomy and Distribution of Genus Hydropsyche (Trichoptera: Hydropsychidae) in Northwestern Algeria.

The main purpose of our paper is to document genus Hydropsyche (Trichoptera: Hydropsychidae) in northwestern Algeria and to provide the larval descriptions of the species set. Larvae, pupae, and imagines were collected from 14 sampling sites over a five-year period (2014-2019). Eight Hydropsyche species have been identified, with Hydropsyche siltalai being a new record for Algeria and North Africa, and Hydropsyche pellucidula a new record for Algeria. A comparison of our checklist with those of the Moroccan Rif and Europe is provided, as well as the geographical distribution of each species. This Algerian Hydropsyche species revision provides precise and reliable taxonomic characters for distinguishing larvae of the species, and a taxonomic key is proposed for their identification. In addition, information regarding their distribution is included.

Endoscopist-directed administration of propofol: a worldwide safety experience.

BACKGROUND & AIMS: Endoscopist-directed propofol sedation (EDP) remains controversial. We sought to update the safety experience of EDP and estimate the cost of using anesthesia specialists for endoscopic sedation. METHODS: We reviewed all published work using EDP. We contacted all endoscopists performing EDP for endoscopy that we were aware of to obtain their safety experience. These complications were available in all patients: endotracheal intubations, permanent neurologic injuries, and death. RESULTS: A total of 646,080 (223,656 published and 422,424 unpublished) EDP cases were identified. Endotracheal intubations, permanent neurologic injuries, and deaths were 11, 0, and 4, respectively. Deaths occurred in 2 patients with pancreatic cancer, a severely handicapped patient with mental retardation, and a patient with severe cardiomyopathy. The overall number of cases requiring mask ventilation was 489 (0.1%) of 569,220 cases with data available. For sites specifying mask ventilation risk by procedure type, 185 (0.1%) of 185,245 patients and 20 (0.01%) of 142,863 patients required mask ventilation during their esophagogastroduodenoscopy or colonoscopy, respectively (P < .001). The estimated cost per life-year saved to substitute anesthesia specialists in these cases, assuming they would have prevented all deaths, was $5.3 million. CONCLUSIONS: EDP thus far has a lower mortality rate than that in published data on endoscopist-delivered benzodiazepines and opioids and a comparable rate to that in published data on general anesthesia by anesthesiologists. In the cases described here, use of anesthesia specialists to deliver propofol would have had high costs relative to any potential benefit.

Ability to reverse deeper levels of unintended sedation.

During gastrointestinal endoscopy, patients may reach a level of sedation that is deeper (i.e. deep sedation) than intended to be (i.e. moderate sedation). In such cases the ability to restore respiratory and cardiovascular function is critical. Supportive measures should be combined with administration of specific pharmacologic antagonists in cases of imminent ventilatory failure. Naloxone rapidly reverses sedation and respiratory depression due to previously administered narcotics, whereas flumazenil overturns the effects of midazolam on the central nervous system. Both agents are administered intravenously in repeated doses according to the patient's response. Caution, however, is required, as the half-lives of these reversal agents are shorter than those of the respective agonists and re-sedation may occur. Acute withdrawal syndromes may take place in chronic users of opiates or benzodiazepine. Product monographs along with administration protocols may be demonstrated as a wall chart, to ascertain their safe and effective use in endoscopy units.

Sedation practices for gastrointestinal endoscopy in Europe, North America, Asia, Africa and Australia.

Gastroscopy and colonoscopy are standard practice for diagnosing upper gastrointestinal and colonic diseases. Sedation improves tolerance of the endoscopic procedures, but may be responsible for about 50% of the complication rate of the examination. Data from countries of the five continents regarding the rate of using sedation for gastrointestinal endoscopy are rare. We performed a literature search (PubMed) to identify published national or international studies and summarize data on the rate of using sedation for diagnostic gastrointestinal endoscopic procedures in countries of Europe, North America, Asia, Africa and Australia. In continents where data were not available, we used mailed questionnaires to endoscopy centers in countries of these continents. Our data indicate that the use of sedation for diagnostic gastrointestinal endoscopy is high in North America and Australia, but varies considerably among countries in Europe, Asia and Africa.

A randomized trial of topical anesthesia comparing lidocaine versus lidocaine plus xylometazoline for unsedated transnasal upper gastrointestinal endoscopy.

BACKGROUND: The optimal topical anesthesia regimen for unsedated transnasal endoscopy is unknown. The addition of a nasal decongestant, such as xylometazoline (X), to a topical anesthestic may improve patient comfort. OBJECTIVE: To determine the effectiveness of lidocaine (L) versus L plus X (LX) for anesthesia in unsedated transnasal endoscopy. METHODS: Consecutive participants of the Aklavik Helicobacter pylori project were prospectively randomly assigned to receive LX or L for unsedated transnasal 4.9 mm ultrathin endoscopy. The primary outcome was overall procedure discomfort on a validated 10-point visual analogue scale (1 = no discomfort, 10 = severe discomfort). Secondary outcomes included pain, endoscope insertion difficulty, gagging, adverse events and encounter times. Results were presented as mean +/- SD, difference in mean, 95% CI. RESULTS: A total of 181 patients were randomly assigned to receive LX (n=94) and L (n=87). Baseline characteristics between the two groups were similar (mean age 40 years, 59% women). Overall, patient procedural discomfort with LX and L were 4.2+/-2.4 versus 3.9+/-2.1, respectively (0.29; 95% CI -0.39 to 0.96). Transnasal insertion difficulty was significantly lower with LX than with L (2.4+/-2.1 versus 3.2+/-2.8, respectively [-0.80; 95% CI -1.54 to -0.06]). Compared with L, the use of LX was associated with significantly less time needed to apply anesthesia (2.4+/-1.8 min versus 3.5+/-2.2 min, respectively [-1.10; 95% CI -1.71 min to -0.50 min]) and less time for insertion (3.2+/-1.8 min versus 3.9+/-2.2 min, respectively [-0.70 min; 95% CI -1.30 min to -0.10 min]). Epistaxis was rare but occurred less frequently with LX (1.1%) than with L (4.6%) (P=0.19). CONCLUSIONS: LX did not improve patient comfort for transnasal endoscopy compared with L alone. However, LX was associated with less difficulty with endoscope transnasal insertion and reduced insertion time. Further studies on the optimal regimen and dosing of anesthesia are required.

Interesting new Chinese species of Leptoceridae and Odontoceridae (Insecta: Trichoptera) from several recent collecting efforts.

In contravention of Article 16.4.2 of the International Code of Zoological Nomenclature (International Trust for Zoological Nomenclature 1999), the repository for the holotypes of these species was not mentioned by Yang et al. (2020), such that the names proposed for these species are presently nomina nuda.

Interesting new Chinese species of Leptoceridae and Odontoceridae (Insecta: Trichoptera) from several recent collecting efforts.

Nine new species of caddisflies are described from southeastern and central China, including 7 species of Leptoceridae: Ceraclea (Ceraclea) megalophyllon Yang Morse sp. n., C. (Athripsodina) aerumnula Yang Morse sp. n., C. (Ath.) lamellata Yang Hu sp. n., Oecetis (Oecetis) discedens Yang Morse sp. n., Oe. (Pleurograpta) spinellosa Yang Hu sp. n., Setodes charax Yang Morse sp. n., and S. scutatus Yang Morse sp. n. Two species of Odontoceridae also are included: Phraepsyche acuminata Yang Morse sp. n. and Psilotreta longicornis Yang Morse sp. n. The male genitalia of all species and female genitalia of C. megalophylla, C. lamellata, Oecetis discedens, and Oe. spinellosa are figured.