Distribution of tuberculosis cases in the state of Paraná: an ecological study, Brazil, 2018-2021.

OBJECTIVE: to analyze the distribution of tuberculosis cases in the state of Paraná, Brazil, between 2018 and 2021. METHODS: this was an ecological study using secondary data obtained from compulsory notifications; detection rates per 100,000 inhabitants were described according to health regions in the state; percentage changes between 2018-2019 and 2020-2021 were calculated. RESULTS: a total of 7,099 cases were registered. The highest rates were observed in the health regions of Paranaguá (52.4/100,000 in 2018-2019; 38.2/100,000 in 2020-2021) and Foz do Iguaçu (34.4/100,000 in 2018-2019; 20.5/100,000 in 2020-2021), and the lowest rates in Irati (6.3/100,000 in 2018-2019; 8.8/100,000 in 2020-2021) and Francisco Beltrão (8.5/100,000 in 2018-2019; 7.6/100,000 in 2020-2021); in 2020-2021, it could be seen a decrease in percentage changes in 18 health regions, while there was an increase in four of them, especially Foz do Iguaçu (-40.5%) and Cianorte (+53.6%). CONCLUSION: high rates were found in the coastal and triple border regions; and there was a decline in detection rates in the pandemic period.

A systematic review about affinity tags for one-step purification and immobilization of recombinant proteins: integrated bioprocesses aiming both economic and environmental sustainability.

The present study reviewed and discussed the promising affinity tags for one-step purification and immobilization of recombinant proteins. The approach used to structure this systematic review was The Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) methodology. The Scopus and Web of Science databases were used to perform the bibliographic survey by which 267 articles were selected. After the inclusion/exclusion criteria and the screening process, from 25 chosen documents, we identified 7 types of tags used in the last 10 years, carbohydrate-binding module tag (CBM), polyhistidine (His-tag), elastin-like polypeptides (ELPs), silaffin-3-derived pentalysine cluster (Sil3k tag), N-acetylmuramidase (AcmA tag), modified haloalkane dehalogenase (HaloTag®), and aldehyde from a lipase polypeptide (Aldehyde tag). The most used bacterial host for expressing the targeted protein was Escherichia coli and the most used expression vector was pET-28a. The results demonstrated two main immobilization and purification methods: the use of supports and the use of self-aggregating tags without the need of support, depending on the tag used. Besides, the chosen terminal for cloning the tag proved to be very important once it could alter enzyme activity. In conclusion, the best tag for protein one-step purification and immobilization was CBM tag, due to the eco-friendly supports that can be provided from industry wastes, the fast immobilization with high specificity, and the reduced cost of the process.

A Scoping Review of Interventions for Family Bereavement Care during the COVID-19 Pandemic.

The death of a loved one is a major stressor, and bereaved people are at a higher risk of negative health effects. This risk is higher during the COVID-19 pandemic, which raises the need for understanding existing bereavement support interventions. This scoping review aimed to map and summarize findings from the existing literature regarding bereavement support interventions (i.e., psychosocial and psychotherapeutic interventions) for family carers of people who died of COVID-19. The Arksey and O'Malley methodological framework was used. Five databases-Medline, PubMed, CINAHL, Scopus, and Web of Science-were searched for articles available from the inception of COVID-19 pandemic (March 2020) to January 2022, following the PRISMA guidelines. Among the 990 studies identified, only seven met this study's inclusion criteria. The analysis comprised three key topics: types of support programmes and bereavement interventions; tools used to measure the outcomes; and evidence of the impacts of the interventions. All studies analysed included interdisciplinary interventions, commonly developed in clinical settings. Support for recently bereaved individuals can entail cognitive behavioural therapy strategies and other tools to educate, guide, support, and promote healthy integration of loss. To mitigate the effects of non-normative family bereavement, we recommend a systematic approach and coordination between organizational settings, including access to informal and professional support, in order to find hope while navigating the aftermath of COVID-19.

Family bereavement care interventions during the COVID-19 pandemic: a scoping review protocol.

INTRODUCTION: The COVID-19 pandemic has caused significant disruptions to daily social routines and to the lived experience of bereaved families. This article outlines the protocol for a scoping review of published studies to evaluate psychosocial and psychotherapeutic interventions intended to help family carers adjust to grief, loss and bereavement due to COVID-19. This review addresses one broad research question: 'What do we know about bereavement support interventions for family carers of COVID-19 victims?' METHODS AND ANALYSIS: The seminal framework by Arksey and O'Malley will guide the review process, which will cover both the qualitative and quantitative scientific literature on grief support during COVID-19. We will search for relevant studies in several databases, namely PubMed, Web of Science, CINAHL Complete, PsycINFO, Scopus and Directory of Open Access Journals. Moreover, we will search the reference lists of included studies and grey literature sources. The database search will be limited to studies from February 2020 (first death by COVID-19) to 1 January 2022. Only literature written in English, Portuguese and Spanish shall be included. Two independent reviewers will screen the literature, select articles and extract data, in an iterative process. Any disagreements will be solved through consensus-based discussion. Results will be reported with descriptive statistics, accompanied by a thematic analysis. ETHICS AND DISSEMINATION: The study will use information acquired from previously published papers and hence does not require ethical approval. This protocol is registered with the Open Science Framework (https://osf.io/bw7fn/). Scoping results will be disseminated via posters and oral presentations to both academic and clinical audiences, as well as through peer-reviewed journals.

Temporal trend, distribution and spatial autocorrelation of leprosy in Brazil: ecological study, 2011 to 2021.

OBJECTIVE: To characterize the temporal trend and spatial behavior of leprosy in Brazil, from 2011 to 2021. METHODS: This is an ecological study, with data from the Notifiable Diseases Information System, obtained in June 2022. The annual detection rate of new leprosy cases per 100 thousand inhabitants was calculated. To estimate the trend of the 2011-2019 and 2011-2021 series, the polynomial regression model was used, testing first-, second-, and third-order polynomials. For spatiality, natural breaks were used and, later, the univariate global and local Moran's indexes. A significance level of 5% was adopted and the analyses were performed using SPSS®, GeoDa®, and QGIS® software. RESULTS: The findings indicated an upward trend in the incidence of leprosy in Brazilian regions and in 20 federative units between 2011 and 2019. However, there was a decrease in most of the country when considering the COVID-19 pandemic years. Spatiality showed that the highest detection rates throughout the period were observed in the North, Midwest, and Northeast regions, with high-risk clusters, and the lowest detection rates in the South and Southeast regions, with low-risk clusters. CONCLUSION: The leprosy detection rate showed an upward trend in Brazil between 2011 and 2019, with greater spatial concentration in the North, Northeast, and Midwest regions. Nevertheless, the study raises an alert for the programmatic sustainability of leprosy control in Brazil, considering the drop in the COVID-19 pandemic, presumably due to the influence of the reorganization of the development of initiatives and provision of services in face of COVID-19.

Post-acute COVID and long-COVID among adults and older adults in the State of Paraná, Brazil: protocol for an ambispective cohort study.

INTRODUCTION: Since 2020, the world has been going through a viral pandemic with a high morbidity and mortality rate along with the potential to evolve from an acute infection to post-acute and long-COVID, which is still in the process of elucidation. Diagnostic and prognostic research is essential to understand the complexity of factors and contexts involving the illness's process. This protocol introduces a study strategy to analyse predictors, sequelae, and repercussions of COVID-19 in adults and older adults with different disease severities in the State of Paraná, Brazil. METHODS AND ANALYSIS: A mixed-methods sequential explanatory design. The quantitative data will be conducted by an ambispective cohort study, which will explore the manifestations of COVID-19 for 18 months, with nearly 3000 participants with confirmed diagnoses of COVID-19 (reverse transcription-PCR test) between March and December of 2020, retrieved from national disease reporting databases, over 18 years old, living in a Brazilian State (Paraná) and who survived the viral infection after being discharged from a health service. Data collection will be conducted through telephone interviews, at two different occasions: the first will be a recall 12 months after the acute phase as a retrospective follow-up, and the second will be another prospective interview, with data of the following 6 months. For the qualitative step, Grounded Theory will be used; participants will be selected from the cohort population. The first sample group will be composed of people who were discharged from the intensive care unit, and other sample groups will be composed according to theoretical saturation. The qualitative data will follow the temporal design and classification of the disease provided for in the cohort. ETHICS AND DISSEMINATION: Ethics approval was granted by the State University of Maringá, under opinion number: 4 165 272 and CAAE: 34787020.0.0000.0104 on 21 July 2020, and Hospital do Trabalhador (Worker's Hospital), which is accountable for the Health Department of the State of Paraná, under opinion number: 4 214 589 and CAAE: 34787020.0.3001.5225 on 15 August 2020. The participants will verbally consent to the research, their consent will be recorded, and the informed consent form will be sent by mail or email. Outcomes will be widely disseminated through peer-reviewed manuscripts, conference presentations, media and reports to related authorities.

Hospitalização de idosos por COVID-19 no Paraná: uma análise de fatores associados / Hospitalización de personas mayores por COVID-19 en Paraná: un análisis de factores asociados / Hospitalization of elderly people due to COVID-19 in the State of Paraná, Brazil: analysis of associated factors

Resumo Objetivo Analisar a prevalência e os fatores associados à hospitalização de idosos com COVID-19 no estado do Paraná, PR, Brasil. Métodos Estudo transversal vinculado à coorte "Acompanhamento Longitudinal de adultos e idosos que receberam alta da internação hospitalar por COVID-19", realizado por meio de informações contidas nas fichas de notificação compulsória do Sistema de Informação de Agravos de Notificação. As análises foram realizadas através de frequências relativas e absolutas, com aplicação do teste de qui-quadrado adotado no modelo de regressão logística. A população do estudo englobou pessoas residentes no Estado do Paraná com idade de 60 anos ou mais, hospitalizadas por COVID-19 no período de março de 2020 a setembro de 2021. Resultados Foi identificada maior prevalência de hospitalização entre idosos com escolaridade igual ou maior a oito anos. Indivíduos não vacinados contra COVID-19 apresentaram maior chance de internação. O sexo masculino apresentou mais chance de admissão em Unidade de Terapia Intensiva em comparação com o sexo feminino. Doenças cardiovasculares, pneumopatia e obesidade aumentaram a prevalência da forma grave da doença. Conclusão Fatores tais como escolaridade e não adesão à vacinação contra COVID-19 podem aumentar o risco de hospitalização pela doença. Pessoas idosas do sexo masculino apresentam maior chance de hospitalização na UTI se comparadas às do sexo feminino; além disso, a não utilização de antivirais pode contribuir para o agravamento do estado de saúde.

Resumen Objetivo Analizar la prevalencia y los factores asociados a la hospitalización de personas mayores por COVID-19 en el estado de Paraná. Métodos Estudio transversal vinculado a la cohorte "Seguimiento longitudinal de adultos y personas mayores que recibieron alta de internación hospitalaria por COVID-19", realizado mediante información contenida en las fichas de notificación obligatoria del Sistema de Información de Agravios de Notificación. Los análisis fueron realizados a través de frecuencias relativas y absolutas, con aplicación de la prueba ji cuadrado adoptada en el modelo de regresión logística. La población del estudio incluyó personas residentes del estado de Paraná, de 60 años o más, hospitalizadas por COVID-19 en el período de marzo de 2020 a septiembre de 2021. Resultados Se identificó mayor prevalencia de hospitalización en personas mayores con escolaridad igual o mayor a ocho años. Individuos no vacunados contra COVID-19 presentaron mayor probabilidad de internación. El sexo masculino presentó más probabilidad de admisión en Unidad de Cuidados Intensivos en comparación con el sexo femenino. Enfermedades cardiovasculares, neumopatía y obesidad aumentaron la prevalencia de la forma grave de la enfermedad. Conclusión Factores tales como escolaridad y no adhesión a la vacunación contra COVID-19 pueden aumentar el riesgo de hospitalización por la enfermedad. Personas mayores de sexo masculino presentaron mayor probabilidad de hospitalización en la UCI al compararlas con las de sexo femenino. Además, la no utilización de antivirales puede contribuir al agravamiento del estado de salud.

Abstract Objective To analyze the prevalence and factors associated with hospitalization of elderly people with COVID-19 in the State of Paraná, PR, Brazil. Methods A cross-sectional study linked to the cohort "Longitudinal Monitoring of adults and elderly people who were discharged from hospital admission due to COVID-19", was carried out using information contained in the compulsory notification forms of the Notifiable Diseases Information System. Analyzes were carried out using relative and absolute frequencies, applying the chi-square test adopted in the logistic regression model. The study population included people aged 60 years or over and residing in the State of Paraná, who were hospitalized for COVID-19 from March 2020 to September 2021. Results A higher hospitalization prevalence was identified among elderly people with eight years of education or more. Individuals not vaccinated against COVID-19 had a greater chance of hospitalization. Males had a greater chance of admission to the Intensive Care Unit compared to females. Cardiovascular diseases, lung disease, and obesity have increased the prevalence of the severe form of the disease. Conclusion Factors such as education and non-adherence to vaccination against COVID-19 can increase the risk of hospitalization due to the disease. Elderly people of the male sex have a greater chance of hospitalization in the ICU compared to the female sex. Furthermore, not using antivirals can contribute to worsening health status.

Distribuição dos casos de tuberculose no Paraná: um estudo ecológico, 2018-2021 / Distribución de casos de tuberculosis en Paraná, Brasil: un estudio ecológico, 2018 a 2021 / Distribution of tuberculosis cases in the state of Paraná: an ecological study, Brazil, 2018-2021

Objetivo: analisar a distribuição dos casos de tuberculose no Paraná, Brasil, entre 2018 e 2021. Métodos: estudo ecológico, sobre dados secundários oriundos de notificações compulsórias; descrição das taxas de detecção por 100 mil habitantes segundo regiões de saúde do estado; cálculo das variações percentuais entre 2018-2019 e 2020-2021. Resultados: foram registrados 7.099 casos, observando-se maiores taxas nas regionais de Paranaguá (52,4/100 mil em 2018-2019; 38,2/100 mil em 2020-2021) e Foz do Iguaçu (34,4/100 mil em 2018-2019; 20,5/100 mil em 2020-2021), e menores em Irati (6,3/100 mil em 2018-2019; 8,8/100 mil em 2020-2021) e Francisco Beltrão (8,5/100 mil em 2018-2019; 7,6/100 mil em 2020-2021); em 2020-2021, houve queda nas variações percentuais dessas taxas em 18 regionais e aumento em quatro, destacando-se, respectivamente, Foz do Iguaçu (-40,5%) e Cianorte (+53,6%). Conclusão: foram observadas taxas elevadas nas regionais do litoral e da tríplice fronteira; houve declínio das taxas de detecção no período pandêmico.

Objective: to analyze the distribution of tuberculosis cases in the state of Paraná, Brazil, between 2018 and 2021. Methods: this was an ecological study using secondary data obtained from compulsory notifications; detection rates per 100,000 inhabitants were described according to health regions in the state; percentage changes between 2018-2019 and 2020-2021 were calculated. Results: a total of 7,099 cases were registered. The highest rates were observed in the health regions of Paranaguá (52.4/100,000 in 2018-2019; 38.2/100,000 in 2020-2021) and Foz do Iguaçu (34.4/100,000 in 2018-2019; 20.5/100,000 in 2020-2021), and the lowest rates in Irati (6.3/100,000 in 2018-2019; 8.8/100,000 in 2020-2021) and Francisco Beltrão (8.5/100,000 in 2018-2019; 7.6/100,000 in 2020-2021); in 2020-2021, it could be seen a decrease in percentage changes in 18 health regions, while there was an increase in four of them, especially Foz do Iguaçu (-40.5%) and Cianorte (+53.6%). Conclusion: high rates were found in the coastal and triple border regions; and there was a decline in detection rates in the pandemic period.

Objetivo: analizar la distribución de casos de tuberculosis en Paraná de 2018 a 2021. Métodos: estudio ecológico utilizando datos secundarios de notificaciones obligatorias. Se describieron las tasas de detección por 100.000 habitantes según las regiones de salud de Paraná. Se calcularon los cambios porcentuales entre 2018-2019 y 2020-2021. Resultados: se registraron 7.099 casos, observándose tasas más altas en las regiones de Paranaguá (52,4/100.000 en 2018-2019 y 38,2/100.000 en 2020-2021) y Foz do Iguaçu (34,4/100.000 en 2018-2019 y 20,5/100.000 en 2020-2021) y menor en Irati (6,3/100.000 en 2018-2019 y 8,8/100.000 en 2020-2021) y Francisco Beltrão (8,5/100.000 en 2018-2019 y 7,6/100.000 en 2020-2021). En 2020-2021, hubo descenso en 18 regiones y aumento en cuatro, con destaque para Foz do Iguaçu (-40,5%) y Cianorte (+53,6%). Conclusión: se encontraron altas tasas para las regiones de la costa y la triple frontera, además de una disminución en la detección durante el período de pandemia.

Análise comparativa do conhecimento de estudantes de enfermagem sobre hiv/aids e sífilis / Análisis comparativo del conocimiento de los estudiantes de enfermería sobre el vih/sida y la sífilis / Comparative analysis of the knowledge of nursing students on hiv/aids and syphilis

Objetivo: analisar o conhecimento de estudantes de enfermagem de diferentes séries da graduação em relação ao HIV/aids e sífilis. Método: pesquisa descritivo-exploratória, desenvolvida com 65 acadêmicos de enfermagem de uma universidade do Sul do Brasil. Os dados foram coletados por instrumento adaptado da Organização Mundial da Saúde. Aplicou-se teste de Kruskal-Wallis e da diferença mínima significativa de Fisher para a comparação. Resultados: houve diferença significativa do conhecimento entre as séries em aspectos específicos das infecções, como: janela imunológica do HIV, fisiopatologia da aids, modo de transmissão da sífilis, método indetectável = intransmissível do HIV e caracterização das fases da sífilis. Conclusão: a aquisição de saberes sobre HIV/aids e sífilis entre estudantes de enfermagem tende a evoluir ao longo da graduação, visto que as últimas séries apresentaram melhores escores. Todavia, o conhecimento prévio dos estudantes das séries iniciais ficou evidente e, por vezes, não se inferiorizou em relação às demais.

Objetivo: analizar el conocimiento de los estudiantes de enfermería de diferentes grados de pregrado en relación con el VIH/SIDA y la sífilis. Método: investigación descriptiva-exploratoria, desarrollada con 65 estudiantes de enfermería de una universidad del sur de Brasil. Los datos fueron recolectados por un instrumento adaptado de la Organización Mundial de la Salud. Se aplicó la prueba de Kruskal-Wallis y la diferencia mínima significativa del pescador para la comparación. Resultados: hubo una diferencia significativa en el conocimiento entre las series en aspectos específicos de las infecciones, tales como: ventana inmunológica del VIH, fisiopatología del sida, modo de transmisión de la sífilis, método indetectable = intransferible del VIH y caracterización de las fases de sífilis. Conclusión: la adquisición de conocimientos sobre VIH/SIDA y sífilis entre los estudiantes de enfermería tiende a evolucionar a lo largo de la graduación, ya que los últimos grados presentaron mejores puntajes. Sin embargo, el conocimiento previo de los alumnos de las notas iniciales era evidente y, en ocasiones, no era inferior en relación a los demás.

Objective: to analyze the knowledge of nursing students from different undergraduate grades in relation to HIV/AIDS and syphilis. Method: descriptive-exploratory research, developed with 65 nursing students from a university in southern Brazil. Data were collected by an instrument adapted from the World Health Organization. Kruskal-Wallis test and fisher significant minimum difference was applied for comparison. Results: there was a significant difference in knowledge between the series in specific aspects of infections, such as: HIV immunological window, aids pathophysiology, mode of transmission of syphilis, undetectable method = non-transferable of HIV and characterization of syphilis phases. Conclusion: the acquisition of knowledge about HIV/AIDS and syphilis among nursing students tends to evolve throughout graduation, since the last grades presented better scores. However, the previous knowledge of the students of the initial grades was evident and, at times, it was not inferior in relation to the other ones.

Temporal trend, distribution and spatial autocorrelation of leprosy in Brazil: ecological study, 2011 to 2021 / Tendência temporal, distribuição e autocorrelação espacial da hanseníase no Brasil: estudo ecológico, 2011 a 2021

ABSTRACT Objective: To characterize the temporal trend and spatial behavior of leprosy in Brazil, from 2011 to 2021. Methods: This is an ecological study, with data from the Notifiable Diseases Information System, obtained in June 2022. The annual detection rate of new leprosy cases per 100 thousand inhabitants was calculated. To estimate the trend of the 2011-2019 and 2011-2021 series, the polynomial regression model was used, testing first-, second-, and third-order polynomials. For spatiality, natural breaks were used and, later, the univariate global and local Moran's indexes. A significance level of 5% was adopted and the analyses were performed using SPSS®, GeoDa®, and QGIS® software. Results: The findings indicated an upward trend in the incidence of leprosy in Brazilian regions and in 20 federative units between 2011 and 2019. However, there was a decrease in most of the country when considering the COVID-19 pandemic years. Spatiality showed that the highest detection rates throughout the period were observed in the North, Midwest, and Northeast regions, with high-risk clusters, and the lowest detection rates in the South and Southeast regions, with low-risk clusters. Conclusion: The leprosy detection rate showed an upward trend in Brazil between 2011 and 2019, with greater spatial concentration in the North, Northeast, and Midwest regions. Nevertheless, the study raises an alert for the programmatic sustainability of leprosy control in Brazil, considering the drop in the COVID-19 pandemic, presumably due to the influence of the reorganization of the development of initiatives and provision of services in face of COVID-19.

RESUMO Objetivo: Caracterizar a tendência temporal e o comportamento espacial da hanseníase no Brasil, de 2011 a 2021. Métodos: Estudo ecológico, com dados do Sistema de Informação de Agravos de Notificação, obtidos em junho de 2022. Calculou-se a taxa de detecção anual de casos novos de hanseníase por 100 mil habitantes. Para estimar a tendência das séries 2011-2019 e 2011-2021, empregou-se o modelo de regressão polinomial, testando polinômios de primeira, segunda e terceira ordem. Para a espacialidade, utilizaram-se as quebras naturais e, posteriormente, as estatísticas univariadas de Moran global e local. Adotou-se o nível de significância de 5% e as análises foram realizadas no Statistical Package for the Social Sciences — SPSS®, GeoDa® e QGIS®. Resultados: Os achados apontaram para a tendência crescente da incidência de hanseníase no Brasil, nas regiões e em 20 unidades da federação entre 2011 e 2019; contudo, houve decréscimo em grande parte do país ao se considerarem os anos pandêmicos. A espacialidade revelou que as maiores taxas de detecção, em todo o período, foram observadas nas Regiões Norte, Centro-Oeste e Nordeste, com clusters de alto risco, e as menores nas Regiões Sul e Sudeste, com aglomerados de baixo risco. Conclusão: A taxa de detecção da hanseníase apresentou tendência crescente no Brasil entre 2011 e 2019, com maior concentração espacial nas Regiões Norte, Nordeste e Centro-Oeste. Entretanto, o estudo traz um alerta para a sustentabilidade programática do controle da hanseníase no Brasil, dada a queda evidenciada na pandemia, presumivelmente por influência da reorganização da oferta de ações e serviços anteposta àCOVID-19.