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1.
J Biol Chem ; 294(11): 3947-3956, 2019 03 15.
Artículo en Inglés | MEDLINE | ID: mdl-30655288

RESUMEN

Human DNA polymerase δ is essential for DNA replication and acts in conjunction with the processivity factor proliferating cell nuclear antigen (PCNA). In addition to its catalytic subunit (p125), pol δ comprises three regulatory subunits (p50, p68, and p12). PCNA interacts with all of these subunits, but only the interaction with p68 has been structurally characterized. Here, we report solution NMR-, isothermal calorimetry-, and X-ray crystallography-based analyses of the p12-PCNA interaction, which takes part in the modulation of the rate and fidelity of DNA synthesis by pol δ. We show that p12 binds with micromolar affinity to the classical PIP-binding pocket of PCNA via a highly atypical PIP box located at the p12 N terminus. Unlike the canonical PIP box of p68, the PIP box of p12 lacks the conserved glutamine; binds through a 2-fork plug made of an isoleucine and a tyrosine residue at +3 and +8 positions, respectively; and is stabilized by an aspartate at +6 position, which creates a network of intramolecular hydrogen bonds. These findings add to growing evidence that PCNA can bind a diverse range of protein sequences that may be broadly grouped as PIP-like motifs as has been previously suggested.


Asunto(s)
ADN Polimerasa III/metabolismo , Antígeno Nuclear de Célula en Proliferación/metabolismo , Secuencias de Aminoácidos , Calorimetría , Dominio Catalítico , ADN Polimerasa III/química , ADN Polimerasa III/aislamiento & purificación , Humanos , Modelos Moleculares , Resonancia Magnética Nuclear Biomolecular , Antígeno Nuclear de Célula en Proliferación/química , Antígeno Nuclear de Célula en Proliferación/aislamiento & purificación
2.
J Mol Recognit ; 33(4): e2826, 2020 04.
Artículo en Inglés | MEDLINE | ID: mdl-31742833

RESUMEN

N-Methyl-D-aspartate (NMDA) receptors are key components in synaptic communication and are highly relevant in central nervous disorders, where they trigger excessive calcium entry into the neuronal cells causing harmful overproduction of nitric oxide by the neuronal nitric oxide synthase (nNOS) protein. Remarkably, NMDA receptor activation is aided by a second protein, postsynaptic density of 95 kDa (PSD95), forming the ternary protein complex NMDA/PSD95/nNOS. To minimize the potential side effects derived from blocking this ternary complex or either of its protein components, a promising approach points to the disruption of the PSD-95/nNOS interaction which is mediated by a PDZ/PDZ domain complex. Since the rational development of molecules targeting such protein-protein interaction relies on energetic and structural information herein, we include a thermodynamic and structural analysis of the PSD95-PDZ2/nNOS-PDZ. Two energetically relevant events are structurally linked to a "two-faced" or two areas of recognition between both domains. First, the assembly of a four-stranded antiparallel ß-sheet between the ß hairpins of nNOS and of PSD95-PDZ2, mainly enthalpic in nature, contributes 80% to the affinity. Second, binding is entropically reinforced by the hydrophobic interaction between side chains of the same nNOS ß-hairpin with the side chains of α2-helix at the binding site of PSD95-PDZ2, contributing the remaining 20% of the total affinity. These results suggest strategies for the future rational design of molecules able to disrupt this complex and constitute the first exhaustive thermodynamic analysis of a PDZ/PDZ interaction.


Asunto(s)
Homólogo 4 de la Proteína Discs Large/metabolismo , Óxido Nítrico Sintasa de Tipo I/metabolismo , Factores de Transcripción/metabolismo , Calorimetría , Rastreo Diferencial de Calorimetría , Homólogo 4 de la Proteína Discs Large/genética , Óxido Nítrico Sintasa de Tipo I/genética , Temperatura , Termodinámica , Factores de Transcripción/genética
3.
Proc Natl Acad Sci U S A ; 112(47): 14599-604, 2015 Nov 24.
Artículo en Inglés | MEDLINE | ID: mdl-26553994

RESUMEN

Enzymes in heteromeric, allosterically regulated complexes catalyze a rich array of chemical reactions. Separating the subunits of such complexes, however, often severely attenuates their catalytic activities, because they can no longer be activated by their protein partners. We used directed evolution to explore allosteric regulation as a source of latent catalytic potential using the ß-subunit of tryptophan synthase from Pyrococcus furiosus (PfTrpB). As part of its native αßßα complex, TrpB efficiently produces tryptophan and tryptophan analogs; activity drops considerably when it is used as a stand-alone catalyst without the α-subunit. Kinetic, spectroscopic, and X-ray crystallographic data show that this lost activity can be recovered by mutations that reproduce the effects of complexation with the α-subunit. The engineered PfTrpB is a powerful platform for production of Trp analogs and for further directed evolution to expand substrate and reaction scope.


Asunto(s)
Evolución Molecular Dirigida , Subunidades de Proteína/metabolismo , Pyrococcus furiosus/enzimología , Triptófano Sintasa/metabolismo , Regulación Alostérica , Aminoácidos/metabolismo , Biocatálisis , Cristalografía por Rayos X , Ingeniería Genética , Ligandos , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Mutación/genética , Subunidades de Proteína/química , Salmonella typhimurium/enzimología , Espectrofotometría Ultravioleta , Especificidad por Sustrato , Triptófano Sintasa/química
4.
J Am Chem Soc ; 139(31): 10769-10776, 2017 08 09.
Artículo en Inglés | MEDLINE | ID: mdl-28708383

RESUMEN

Derivatives of the amino acid tryptophan (Trp) serve as precursors for the chemical and biological synthesis of complex molecules with a wide range of biological properties. Trp analogues are also valuable as building blocks for medicinal chemistry and as tools for chemical biology. While the enantioselective synthesis of Trp analogues is often lengthy and requires the use of protecting groups, enzymes have the potential to synthesize such products in fewer steps and with the pristine chemo- and stereoselectivity that is a hallmark of biocatalysis. The enzyme TrpB is especially attractive because it can form Trp analogues directly from serine (Ser) and the corresponding indole analogue. However, many potentially useful substrates, including bulky or electron-deficient indoles, are poorly accepted. We have applied directed evolution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate a suite of catalysts for the synthesis of previously intractable Trp analogues. For the most challenging substrates, such as nitroindoles, the key to improving activity lay in the mutation of a universally conserved and mechanistically important residue, E104. The new catalysts express at high levels (>200 mg/L of Escherichia coli culture) and can be purified by heat treatment; they can operate up to 75 °C (where solubility is enhanced) and can synthesize enantiopure Trp analogues substituted at the 4-, 5-, 6-, and 7-positions, using Ser and readily available indole analogues as starting materials. Spectroscopic analysis shows that many of the activating mutations suppress the decomposition of the active electrophilic intermediate, an amino-acrylate, which aids in unlocking the synthetic potential of TrpB.


Asunto(s)
Triptófano Sintasa/metabolismo , Triptófano/metabolismo , Biocatálisis , Triptófano/análogos & derivados , Triptófano Sintasa/química
5.
Biochemistry ; 55(51): 7043-7046, 2016 Dec 27.
Artículo en Inglés | MEDLINE | ID: mdl-27935677

RESUMEN

Tryptophan synthase (TrpS) catalyzes the final steps in the biosynthesis of l-tryptophan from l-serine (Ser) and indole-3-glycerol phosphate (IGP). We report that native TrpS can also catalyze a productive reaction with l-threonine (Thr), leading to (2S,3S)-ß-methyltryptophan. Surprisingly, ß-substitution occurs in vitro with a 3.4-fold higher catalytic efficiency for Ser over Thr using saturating indole, despite a >82000-fold preference for Ser in direct competition using IGP. Structural data identify a novel product binding site, and kinetic experiments clarify the atypical mechanism of specificity: Thr binds efficiently but decreases the affinity for indole and disrupts the allosteric signaling that regulates the catalytic cycle.


Asunto(s)
Proteínas Arqueales/metabolismo , Vías Biosintéticas , Glicerofosfatos/metabolismo , Serina/metabolismo , Triptófano Sintasa/metabolismo , Triptófano/biosíntesis , Regulación Alostérica , Proteínas Arqueales/química , Sitios de Unión , Biocatálisis , Cristalografía por Rayos X , Glicerofosfatos/química , Indoles/química , Indoles/metabolismo , Cinética , Modelos Moleculares , Estructura Molecular , Unión Proteica , Dominios Proteicos , Subunidades de Proteína/química , Subunidades de Proteína/metabolismo , Pyrococcus furiosus/enzimología , Serina/química , Espectrofotometría , Especificidad por Sustrato , Treonina/química , Treonina/metabolismo , Triptófano/análogos & derivados , Triptófano/química , Triptófano/genética , Triptófano/metabolismo , Triptófano Sintasa/química
6.
Angew Chem Int Ed Engl ; 55(38): 11577-81, 2016 09 12.
Artículo en Inglés | MEDLINE | ID: mdl-27510733

RESUMEN

Naturally occurring enzyme homologues often display highly divergent activity with non-natural substrates. Exploiting this diversity with enzymes engineered for new or altered function, however, is laborious because the engineering must be replicated for each homologue. A small set of mutations of the tryptophan synthase ß-subunit (TrpB) from Pyrococcus furiosus, which mimics the activation afforded by binding of the α-subunit, was demonstrated to have a similar activating effect in different TrpB homologues with as little as 57 % sequence identity. Kinetic and spectroscopic analyses indicate that the mutations function through the same mechanism: mimicry of α-subunit binding. From these enzymes, we identified a new TrpB catalyst that displays a remarkably broad activity profile in the synthesis of 5-substituted tryptophans. This demonstrates that allosteric activation can be recapitulated throughout a protein family to explore natural sequence diversity for desirable biocatalytic transformations.


Asunto(s)
Triptófano Sintasa/genética , Triptófano Sintasa/metabolismo , Regulación Alostérica , Biocatálisis , Cinética , Mutagénesis Sitio-Dirigida , Unión Proteica , Ingeniería de Proteínas , Subunidades de Proteína/química , Subunidades de Proteína/genética , Subunidades de Proteína/metabolismo , Pyrococcus furiosus/enzimología , Triptófano Sintasa/química
7.
Int J Biol Macromol ; 274(Pt 1): 133233, 2024 Jun 18.
Artículo en Inglés | MEDLINE | ID: mdl-38901510

RESUMEN

The ubiquitin E2 variant domain of TSG101 (TSG101-UEV) plays a pivotal role in protein sorting and virus budding by recognizing PTAP motifs within ubiquitinated proteins. Disrupting TSG101-UEV/PTAP interactions has emerged as a promising strategy for the development of novel host-oriented antivirals with a broad spectrum of action. Nonetheless, finding inhibitors with good properties as therapeutic agents remains a challenge since the key determinants of binding affinity and specificity are still poorly understood. Here we present a detailed thermodynamic, structural, and dynamic characterization viral PTAP Late domain recognition by TSG101-UEV, combining isothermal titration calorimetry, X-ray diffraction structural studies, molecular dynamics simulations, and computational analysis of intramolecular communication pathways. Our analysis highlights key contributions from conserved hydrophobic contacts and water-mediated hydrogen bonds at the PTAP binding interface. We have identified additional electrostatic hotspots adjacent to the core motif that modulate affinity. Using competitive phage display screening we have improved affinity by 1-2 orders of magnitude, producing novel peptides with low micromolar affinities that combine critical elements found in the best natural binders. Molecular dynamics simulations revealed that optimized peptides engage new pockets on the UEV domain surface. This study provides a comprehensive view of the molecular forces directing TSG101-UEV recognition of PTAP motifs, revealing that binding is governed by conserved structural elements yet tuneable through targeted optimization. These insights open new venues to design inhibitors targeting TSG101-dependent pathways with potential application as novel broad-spectrum antivirals.

8.
Biophys J ; 103(4): 738-47, 2012 Aug 22.
Artículo en Inglés | MEDLINE | ID: mdl-22947935

RESUMEN

The temperature-induced misfolding pathway of PDZ3, the third PDZ domain of the PSD95 neuronal protein, is populated by a trimeric ß-sheet-rich intermediate state that leads to a stepwise and reversible formation of supramacromolecular structures. Using FTIR, we have found that misfolding of this pathway is not due to different ensembles of a variety of precursors, but comes mainly from the interconversion of a flexible ß-sheet of the domain to wormlike fibrils. The appearance of the wormlike fibril FTIR component is also accompanied by a slight decrease of the band that corresponds to loops in the native state, whereas the rest of the regular elements of secondary structure are fairly well maintained upon misfolding. Transmission electron microscope micrographs have confirmed the presence of wormlike fibrils upon heating at 60°C, where the trimeric intermediate is maximally populated. Toxicity assays in the human neuroblastoma cell line SH-SY5Y show that cytotoxicity increases as the aggregation pathway proceeds. NMR analysis of chemical shifts as a function of temperature has revealed, as one of the main conformational aspects of such an interconversion at the residue level, that the ß-sheet arrangement around strand ß3 promotes the change that drives misfolding of the PDZ3 domain.


Asunto(s)
Proteínas de la Membrana/química , Dominios PDZ , Pliegue de Proteína , Multimerización de Proteína , Secuencia de Aminoácidos , Línea Celular Tumoral , Humanos , Proteínas de la Membrana/toxicidad , Modelos Moleculares , Datos de Secuencia Molecular , Estructura Secundaria de Proteína
9.
Adv Protein Chem Struct Biol ; 130: 161-188, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35534107

RESUMEN

Within the modular protein domains there are five families that recognize proline-rich sequences: SH3, WW, EVH1, GYF and UEV domains. This chapter reviews the main strategies developed for the design of ligands for these families, including peptides, peptidomimetics and drugs. We also describe some studies aimed to understand the molecular reasons responsible for the intrinsic affinity and specificity of these domains.


Asunto(s)
Péptidos , Prolina , Sitios de Unión , Humanos , Ligandos , Péptidos/química , Prolina/química , Prolina/metabolismo , Unión Proteica , Dominios Proteicos
10.
Int J Biol Macromol ; 207: 308-323, 2022 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-35257734

RESUMEN

The recognition of PPxY viral Late domains by the third WW domain of the human HECT-E3 ubiquitin ligase NEDD4 (NEDD4-WW3) is essential for the budding of many viruses. Blocking these interactions is a promising strategy to develop broad-spectrum antivirals. As all WW domains, NEDD4-WW3 is a challenging therapeutic target due to the low binding affinity of its natural interactions, its high conformational plasticity, and its complex thermodynamic behavior. In this work, we set out to investigate whether high affinity can be achieved for monovalent ligands binding to the isolated NEDD4-WW3 domain. We show that a competitive phage-display set-up allows for the identification of high-affinity peptides showing inhibitory activity of viral budding. A detailed biophysical study combining calorimetry, nuclear magnetic resonance, and molecular dynamic simulations reveals that the improvement in binding affinity does not arise from the establishment of new interactions with the domain, but is associated to conformational restrictions imposed by a novel C-terminal -LFP motif in the ligand, unprecedented in the PPxY interactome. These results, which highlight the complexity of WW domain interactions, provide valuable insight into the key elements for high binding affinity, of interest to guide virtual screening campaigns for the identification of novel therapeutics targeting NEDD4-WW3 interactions.


Asunto(s)
Bacteriófagos , Complejos de Clasificación Endosomal Requeridos para el Transporte , Secuencias de Aminoácidos , Antivirales , Bacteriófagos/metabolismo , Complejos de Clasificación Endosomal Requeridos para el Transporte/metabolismo , Humanos , Ligandos , Ubiquitina-Proteína Ligasas Nedd4/metabolismo , Unión Proteica , Ubiquitina-Proteína Ligasas/metabolismo
11.
Cancers (Basel) ; 14(11)2022 May 26.
Artículo en Inglés | MEDLINE | ID: mdl-35681628

RESUMEN

Natural phenolic compounds have gained momentum for the prevention and treatment of cancer, but their antitumoral mechanism of action is not yet well understood. In the present study, we screened the antitumoral potential of several phenolic compounds in a cellular model of colorectal cancer (CRC). We selected gallic acid (GA) as a candidate in terms of potency and selectivity and extensively evaluated its biological activity. We report on the role of GA as a ligand of DNA G-quadruplexes (G4s), explaining several of its antitumoral effects, including the transcriptional inhibition of ribosomal and CMYC genes. In addition, GA shared with other established G4 ligands some effects such as cell cycle arrest, nucleolar stress, and induction of DNA damage. We further confirmed the antitumoral and G4-stabilizing properties of GA using a xenograft model of CRC. Finally, we succinctly demonstrate that GA could be explored as a therapeutic agent in a patient cohort with CRC. Our work reveals that GA, a natural bioactive compound present in the diet, affects gene expression by interaction with G4s both in vitro and in vivo and paves the way towards G4s targeting with phenolic compounds.

12.
Biomolecules ; 11(8)2021 07 21.
Artículo en Inglés | MEDLINE | ID: mdl-34439737

RESUMEN

PDZ domains are binding modules mostly involved in cell signaling and cell-cell junctions. These domains are able to recognize a wide variety of natural targets and, among the PDZ partners, viruses have been discovered to interact with their host via a PDZ domain. With such an array of relevant and diverse interactions, PDZ binding specificity has been thoroughly studied and a traditional classification has grouped PDZ domains in three major specificity classes. In this work, we have selected four human PDZ domains covering the three canonical specificity-class binding mode and a set of their corresponding binders, including host/natural, viral and designed PDZ motifs. Through calorimetric techniques, we have covered the entire cross interactions between the selected PDZ domains and partners. The results indicate a rather basic specificity in each PDZ domain, with two of the domains that bind their cognate and some non-cognate ligands and the two other domains that basically bind their cognate partners. On the other hand, the host partners mostly bind their corresponding PDZ domain and, interestingly, the viral ligands are able to bind most of the studied PDZ domains, even those not previously described. Some viruses may have evolved to use of the ability of the PDZ fold to bind multiple targets, with resulting affinities for the virus-host interactions that are, in some cases, higher than for host-host interactions.


Asunto(s)
Dominios PDZ , Proteínas , Sitios de Unión , Humanos , Ligandos , Unión Proteica , Estructura Terciaria de Proteína , Proteínas/química , Proteínas/metabolismo
13.
Biophys J ; 99(1): 263-72, 2010 Jul 07.
Artículo en Inglés | MEDLINE | ID: mdl-20655855

RESUMEN

The equilibrium unfolding at neutral pH of the third PDZ domain of PSD95, as followed by DSC, is characterized by the presence of an equilibrium intermediate with clear signs of oligomerization. DLS and SEC measurements indicate that at 60-70 degrees C small oligomers populate, showing a typical beta-sheet far-UV CD spectrum. These intermediate species lead to the formation of rodlike particulates of approximately 12 nm, which remain in solution after 2 weeks incubation and grow until they adopt annular/spherical shapes of approximately 50 nm and protofibrils, which are subsequently fully transformed into fibrils. The fibrils can also disaggregate after the addition of 1:1 buffer dilution followed by cooling to room temperature, thus returning to the initial monomeric state. Growth kinetics, as shown by ThT and ANS fluorescence, show that the organization of the different supramacromolecular structures comes from a common nucleation unit, the small oligomers, which organize themselves before reaching the incubation temperature of 60 degrees C. Our experiments point toward the existence of a well-defined reversible, stepwise, and downhill organization of the processes involved in the association-dissociation of the intermediate. We estimate the enthalpy change accompanying the association-dissociation equilibria to be 130 kJ x mol(-1). Furthermore, the coalescence under essentially reversible conditions of different kinds of supramacromolecular assemblies renders this protein system highly interesting for biophysical studies aimed at our further understanding of amyloid pathological conditions.


Asunto(s)
Multimerización de Proteína , Rastreo Diferencial de Calorimetría , Guanilato-Quinasas/química , Guanilato-Quinasas/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Dominios PDZ , Desnaturalización Proteica , Pliegue de Proteína , Análisis Espectral , Temperatura , Termodinámica
14.
J Struct Biol ; 170(3): 565-9, 2010 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-20227506

RESUMEN

The crystal structure of the third PDZ domain of the neuronal post-synaptic density-95 protein (PSD95-PDZ3, residues 302-402) has been solved at 1.4 and 1.35A from two different crystal forms. These structures lack the cloning artefact present in the carboxyl terminal sequence of the former crystallographic structures and they belong to the space groups P4(3) and P1. The new PDZ structures are identical between the two crystal forms and among the four chains of the P1 crystal form. When we compare the new structures with the previous ones, some important conformational differences in the C-terminal alpha-helix and in the loop connecting beta2 and beta3 strands have been found. Additionally, the high resolution of the new structures has allowed us to indentify a succinimide residue at the position corresponding to Asp332 in the beta2-beta3 loop, which may contribute to the alternate conformation of this loop, and at the same time, to the interaction between residues from this loop and the C-terminal alpha-helix. Thus, these features would have implications in the recently proposed allosteric role of this third alpha-helix in the binding of the carboxyl terminal fragments to the PSD95-PDZ3.


Asunto(s)
Péptidos y Proteínas de Señalización Intracelular/química , Proteínas de la Membrana/química , Dominios PDZ , Sitio Alostérico , Ácido Aspártico/química , Cristalografía por Rayos X , Modelos Moleculares , Proteínas del Tejido Nervioso/química , Neuronas/química , Conformación Proteica , Estructura Cuaternaria de Proteína , Proteínas Recombinantes/química , Electricidad Estática
15.
Life (Basel) ; 10(8)2020 Jul 27.
Artículo en Inglés | MEDLINE | ID: mdl-32726937

RESUMEN

The PDZ domain (PSD95-Discs large-ZO1) is a widespread modular domain present in the living organisms. A prevalent function in the PDZ family is to serve as scaffolding and adaptor proteins connecting multiple partners in signaling pathways. An explanation of the flexible functionality in this domain family, based just on a static perspective of the structure-activity relationship, might fall short. More dynamic and conformational aspects in the protein fold can be the reasons for such functionality. Folding studies indeed showed an ample and malleable folding landscape for PDZ domains where multiple intermediate states were experimentally detected. Allosteric phenomena that resemble energetic coupling between residues have also been found in PDZ domains. Additionally, several PDZ domains are modulated by post-translational modifications, which introduce conformational switches that affect binding. Altogether, the ability to connect diverse partners might arise from the intrinsic plasticity of the PDZ fold.

16.
Acta Crystallogr D Struct Biol ; 75(Pt 4): 381-391, 2019 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-30988255

RESUMEN

PDZ domains are protein-protein recognition modules that interact with other proteins through short sequences at the carboxyl terminus. These domains are structurally characterized by a conserved fold composed of six ß-strands and two α-helices. The third PDZ domain of the neuronal postsynaptic density protein 95 has an additional α-helix (α3), the role of which is not well known. In previous structures, a succinimide was identified in the ß2-ß3 loop instead of Asp332. The presence of this modified residue results in conformational changes in α3. In this work, crystallographic structures of the following have been solved: a truncated form of the third PDZ domain of the neuronal postsynaptic density protein 95 from which α3 has been removed, D332P and D332G variants of the protein, and a new crystal form of this domain showing the binding of Asp332 to the carboxylate-binding site of a symmetry-related molecule. Crystals of the wild type and variants were obtained in different space groups, which reflects the conformational plasticity of the domain. Indeed, the overall analysis of these structures suggests that the conformation of the ß2-ß3 loop is correlated with the fold acquired by α3. The alternate conformation of the ß2-ß3 loop affects the electrostatics of the carboxylate-binding site and might modulate the binding of different PDZ-binding motifs.


Asunto(s)
Cristalografía por Rayos X/métodos , Homólogo 4 de la Proteína Discs Large/química , Dominios PDZ , Conformación Proteica , Sitios de Unión , Dominio Catalítico , Homólogo 4 de la Proteína Discs Large/metabolismo , Humanos , Modelos Moleculares , Estructura Molecular , Unión Proteica
17.
ACS Chem Biol ; 14(10): 2315-2326, 2019 10 18.
Artículo en Inglés | MEDLINE | ID: mdl-31479228

RESUMEN

The proliferating cell nuclear antigen (PCNA)-associated factor p15PAF is a nuclear protein that acts as a regulator of DNA repair during DNA replication. The p15PAF gene is overexpressed in several types of human cancer, and its function is regulated by monoubiquitination of two lysines (K15 and K24) at the protein N-terminal region. We have previously shown that p15PAF is an intrinsically disordered protein which partially folds upon binding to PCNA and independently contacts DNA through its N-terminal tail. Here we present an NMR conformational characterization of p15PAF monoubiquitinated at both K15 and K24 via a disulfide bridge mimicking the isopeptide bond. We show that doubly monoubiquitinated p15PAF is monomeric, intrinsically disordered, and binds to PCNA as nonubiquitinated p15PAF does but interacts with DNA with reduced affinity. Our SAXS-derived conformational ensemble of doubly monoubiquitinated p15PAF shows that the ubiquitin moieties, separated by eight disordered residues, form transient dimers because of the high local effective ubiquitin concentration. This observation and the sequence similarity with histone H3 N-terminal tail suggest that doubly monoubiquitinated p15PAF is a binding target of DNA methyl transferase Dnmt1, as confirmed by calorimetry. Therefore, doubly monoubiquitinated p15PAF directly interacts with PCNA and recruits Dnmt1 for maintenance of DNA methylation during replication.


Asunto(s)
ADN (Citosina-5-)-Metiltransferasa 1/metabolismo , Proteínas de Unión al ADN/metabolismo , Ubiquitinación , ADN/metabolismo , Proteínas de Unión al ADN/química , Escherichia coli , Humanos , Proteínas Intrínsecamente Desordenadas/química , Proteínas Intrínsecamente Desordenadas/metabolismo , Antígeno Nuclear de Célula en Proliferación/química , Antígeno Nuclear de Célula en Proliferación/metabolismo , Unión Proteica , Conformación Proteica , Dominios Proteicos , Procesamiento Proteico-Postraduccional
18.
Amyloid ; 24(3): 167-175, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28699800

RESUMEN

ScFv-h3D6 is a single-chain variable fragment derived from the monoclonal antibody bapineuzumab that prevents Aß-induced cytotoxicity by capturing Aß oligomers. The benefits of scFv-h3D6 treatment in Alzheimer's disease are known at the behavioural, cellular and molecular levels in the 3xTg-AD mouse model. Antibody-based therapeutics are only stable in a limited temperature range, so their benefit in vivo depends on their capability for maintaining the proper fold. Here, we have stabilized the scFv-h3D6 folding by introducing the mutation VH-K64R and combining it with the previously described elongation of the VL domain (C3). The stabilities of the different scFv-h3D6 constructs were calculated from urea and thermal denaturation followed by Trp-fluorescence, CD and DSC and resulted in the order C3 > K64R/C3 > VH-K64R ≥ scFv-h3D6; showing that the combination of both mutations was not additive, instead they partially cancelled each other. The three mutants assayed showed a decreased aggregation tendency but maintained their capability to aggregate in the form of worm-like fibrils, basis of the protective effect of scFv-h3D6. Cytotoxicity assays showed that all the mutants recovered cell viability of Aß-treated neuroblastoma cell cultures in a dose-dependent manner and with efficiencies that correlated with stability, therefore improving the therapeutic ability of this antibody.


Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Péptidos beta-Amiloides/antagonistas & inhibidores , Fragmentos de Péptidos/antagonistas & inhibidores , Anticuerpos de Cadena Única/farmacología , Enfermedad de Alzheimer/metabolismo , Péptidos beta-Amiloides/química , Péptidos beta-Amiloides/metabolismo , Línea Celular Tumoral , Humanos , Fragmentos de Péptidos/química , Fragmentos de Péptidos/metabolismo , Estabilidad Proteica , Anticuerpos de Cadena Única/química , Anticuerpos de Cadena Única/genética
19.
Sci Rep ; 6: 19242, 2016 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-26754462

RESUMEN

PDZ domains are protein-protein interaction modules sharing the same structural arrangement. To discern whether they display common features in their unfolding/misfolding behaviour we have analyzed in this work the unfolding thermodynamics, together with the misfolding kinetics, of the PDZ fold using three archetypical examples: the second and third PDZ domains of the PSD95 protein and the Erbin PDZ domain. Results showed that all domains passed through a common intermediate, which populated upon unfolding, and that this in turn drove the misfolding towards worm-like fibrillar structures. Thus, the unfolding/misfolding behaviour appears to be shared within these domains. We have also analyzed how this landscape can be modified upon the inclusion of extra-elements, as it is in the nNOS PDZ domain, or the organization of swapped species, as happens in the second PDZ domain of the ZO2 protein. Although the intermediates still formed upon thermal unfolding, the misfolding was prevented to varying degrees.


Asunto(s)
Modelos Moleculares , Dominios PDZ , Conformación Proteica , Pliegue de Proteína , Desplegamiento Proteico , Secuencia de Aminoácidos , Rastreo Diferencial de Calorimetría , Espectroscopía Infrarroja por Transformada de Fourier
20.
PLoS One ; 9(2): e90030, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24587199

RESUMEN

The relative promiscuity of hub proteins such as postsynaptic density protein-95 (PSD-95) can be achieved by alternative splicing, allosteric regulation, and post-translational modifications, the latter of which is the most efficient method of accelerating cellular responses to environmental changes in vivo. Here, a mutational approach was used to determine the impact of phosphorylation and succinimidation post-translational modifications on the binding affinity of the postsynaptic density protein-95/discs large/zonula occludens-1 (PDZ3) domain of PSD-95. Molecular dynamics simulations revealed that the binding affinity of this domain is influenced by an interplay between salt-bridges linking the α3 helix, the ß2-ß3 loop and the positively charged Lys residues in its high-affinity hexapeptide ligand KKETAV. The α3 helix is an extra structural element that is not present in other PDZ domains, which links PDZ3 with the following SH3 domain in the PSD-95 protein. This regulatory mechanism was confirmed experimentally via thermodynamic and NMR chemical shift perturbation analyses, discarding intra-domain long-range effects. Taken together, the results presented here reveal the molecular basis of the regulatory role of the α3 extra-element and the effects of post-translational modifications of PDZ3 on its binding affinity, both energetically and dynamically.


Asunto(s)
Proteínas de la Membrana/química , Proteínas de la Membrana/metabolismo , Dominios PDZ , Procesamiento Proteico-Postraduccional , Amidas/metabolismo , Secuencia de Aminoácidos , Ligandos , Proteínas de la Membrana/genética , Simulación de Dinámica Molecular , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Mutación , Fosforilación , Unión Proteica , Estructura Secundaria de Proteína , Electricidad Estática , Succinatos/metabolismo , Termodinámica
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