RESUMEN
BACKGROUND: The most crucial clinical problem of Eosinophilic Otitis Media (EOM) is sensorineural hearing loss. A previous report revealed that repeated antigen stimulation of middle ear causes eosinophilic inflammation not only in the middle ear but also in the inner ear. OBJECTIVE: The purpose of the present study was to elucidate the mechanism of eosinophil infiltration to the inner ear in the animal model of EOM. METHODS: Continuous OVA stimulation to the middle ear of guinea pigs was performed for 7 days, 14 days, and 28 days. Histological observation was made for eosinophil infiltration and morphological change of the inner ear. Immunostaining for eotaxin and RANTES was performed to study immunoreactivity of those chemokines. RESULTS: In the 7-day stimulation side, a few eosinophils were found in the scala tympani, without obvious morphological damage of the inner ear. Moreover, immunoreactivity of both eotaxin and RANTES was significantly higher in the OVA stimulation sides than control sides. For both eotaxin and RANTES, the number of immunopositive cells was significantly increased in the 14-day stimulation side over the 7-day stimulation side. CONCLUSIONS: Eotaxin and RANTES seem to play some important roles for the eosinophil infiltration in the middle and inner ear of model animal of EOM.
Asunto(s)
Quimiocina CCL5/metabolismo , Oído Interno/inmunología , Eosinofilia/inmunología , Otitis Media/inmunología , Animales , Quimiocina CCL11/metabolismo , Modelos Animales de Enfermedad , Oído Interno/metabolismo , Oído Medio/metabolismo , Eosinofilia/metabolismo , Cobayas , Otitis Media/metabolismo , Hueso Temporal/metabolismoRESUMEN
CONCLUSION: As the periods of intratympanic injection of ovalbumin (OVA) to the middle ear became longer, marked eosinophil infiltration in the perilymphatic space was observed. Moreover severe morphological damage of the organ of Corti was observed in the 28-day antigen-stimulation side. These results indicate that eosinophilic inflammation occurred in the inner ear and caused profound hearing loss. OBJECTIVE: The purpose of the present study was to elucidate the inner ear damage in a new animal model of eosinophilic otitis media (EOM) which we recently constructed. METHODS: We constructed the animal model of EOM by intraperitoneal and intratympanic injection of OVA. Infiltrating cells and the inner ear damage were examined by histological study. RESULTS: In the inner ear, a few eosinophils were seen in the scala tympani of the organ of Corti and the dilation of capillaries of the stria vascularis was observed in the 7-day stimulation side. In the 14-day antigen stimulation side, some eosinophils and macrophages were seen in not only the scala tympani but also the scala vestibule. In the 28-day antigen-stimulation side, severe morphological damage of the organ of Corti and many eosinophils, red blood cells, and plasma cells infiltrating the perilymph were observed.
Asunto(s)
Modelos Animales de Enfermedad , Oído Interno/patología , Eosinofilia/patología , Otitis Media/patología , Animales , Acueducto Coclear/inmunología , Acueducto Coclear/patología , Oído Interno/inmunología , Oído Medio , Eosinofilia/inmunología , Eosinófilos/inmunología , Eosinófilos/patología , Cobayas , Inyecciones , Inyecciones Intraperitoneales , Recuento de Leucocitos , Macrófagos/inmunología , Macrófagos/patología , Órgano Espiral/inmunología , Órgano Espiral/patología , Otitis Media/inmunología , Ovalbúmina/administración & dosificación , Ovalbúmina/inmunología , Perilinfa/inmunología , Ventana Redonda/inmunología , Ventana Redonda/patología , Rampa Timpánica/inmunología , Rampa Timpánica/patología , Estría Vascular/inmunología , Estría Vascular/patologíaRESUMEN
CONCLUSION: We investigated the localization of periostin in middle ear specimens from patients with eosinophilic otitis media (EOM) and from a newly constructed animal model for EOM. Periostin-positive immunostaining was observed in the middle ear sections obtained from the EOM patients. Immunoreactivity for periostin was also seen in the animal model. These results suggest that periostin plays an important role in subepithelial fibrosis in the middle ear in EOM. OBJECTIVE: The purpose of the present study was to investigate the role of periostin in the middle ear of EOM patients and an animal model. METHODS: Histological and immunohistochemical analyses for periostin were carried out in the middle ear specimens of six EOM patients with/without asthma. An animal model of EOM was constructed by intraperitoneal and topical sensitization with ovalbumin (OVA). Histological and immunocytochemical analyses for periostin were also performed in this model. RESULTS: Immunoreactivities for periostin were observed in the basement membrane and extracellular matrix of the middle ear sections obtained from all EOM patients with/without asthma. In the animal model, eosinophil infiltration and middle ear mucosa thickness were observed. Moreover, periostin-positive immunostaining was shown in the extracellular matrix of the middle ear mucosa on the side topically boosted by OVA.