Creation of Chemically Tri-Layered Collagen Crosslinked Membranes and Their Comparison with Ionically Tri-Layered Chitosan Crosslinked Membranes to Study Human Skin Properties.

In 2009, a new European regulation came into force that forbade the use of animals in the cosmetics industry. As a result, new alternatives were sought, taking into account the new ethical considerations. The main objective of this article is to continue a line of research that aims to build a physical model of skin from a biomaterial scaffold composed of collagen, chitosan or a combination to investigate whether they offer similar behavior to human skin. Collagen, the major component in the dermis, was crosslinked with glutaraldehyde (GTA) to develop three formulations for studying some properties of the skin through rheological tests like swelling index, elasticity or water loss. In addition, this article makes a comparison with the results obtained in the previous article where the membranes were made of chitosan and tripolyphosphate (TPP). The results obtained highlight that the tri-layered membranes scaffold better than the mono-layered ones to increase the elastic modulus (G') and the permeability. Furthermore, they offer a protective effect against water loss compared to mono-layered membranes. As regards chitosan membranes, these have a higher G' modulus than collagen membranes when the degree of deacetylation (DDA) is 85%. However, collagen membranes are more elastic when the DDA of chitosan is 76%, and their linear viscoelastic limit (LVL) doubles that of chitosan membranes, both for the degree of acetylation of 76 and 85%.

Full-duplex transmission of multi-Gb/s subcarrier multiplexing and 5G NR signals in 39 GHz band over fiber and space.

We propose a stable full-duplex transmission of millimeter-wave signals over a hybrid single-mode fiber (SMF) and free-space optics (FSO) link for the fifth-generation (5G) radio access networks to accelerate the Industry 4.0 transformation. For the downlink (DL), we transmit 39 GHz subcarrier multiplexing (SCM) signals using variable quadrature amplitude modulation (QAM) allocations for multi-user services. As a proof of operation, we experimentally demonstrate the transmission of 3 Gb/s SCM signals (1 Gb/s per user) over a hybrid system consisting of a 10 km SMF and 1.2 m FSO link. For the uplink (UL), satisfactory performance for the transmission of 2.4 Gb/s 5G new radio (NR) signal at 37 GHz over the hybrid system is experimentally confirmed for the first time, to the best of our knowledge. The measured error vector magnitudes for both DL and UL signals using 4/16/64-QAM formats are well below the third generation partnership project (3GPP) requirements. We also further evaluate by simulation the full-duplex transmission over the system in terms of received optical and RF powers and bit error rate performance. A wireless radio distance of approximately 200 m, which is sufficient for 5G small-cell networks, is estimated for both DL and UL direction under the heavy rain condition, based on the available data from Spain. Furthermore, simulation for the DL direction is conducted to verify the superior performance of the system using variable QAM allocation over uniform QAM allocation. Using a variable modulation allocation, up to five users (2 Gb/s per user) can be transmitted over a hybrid 10 km SMF and 150 m FSO link.

ICBM-OCEAN: Processing Ultrahigh-Resolution Mass Spectrometry Data of Complex Molecular Mixtures.

Untargeted molecular analyses of complex mixtures are relevant for many fields of research, including geochemistry, pharmacology, and medicine. Ultrahigh-resolution mass spectrometry is one of the most powerful tools in this context. The availability of open scripts and online tools for specific data processing steps such as noise removal or molecular formula assignment is growing, but an integrative tool where all crucial steps are reproducibly evaluated and documented is lacking. We developed a novel, server-based tool (ICBM-OCEAN, Institute for Chemistry and Biology of the Marine Environment, Oldenburg-complex molecular mixtures, evaluation & analysis) that integrates published and novel approaches for standardized processing of ultrahigh-resolution mass spectrometry data of complex molecular mixtures. Different from published approaches, we offer diagnostic and validation tools for all relevant steps. Among other features, we included objective and reproducible reduction of noise and systematic errors, spectra recalibration and alignment, and identification of likeliest molecular formulas. With 15 chemical elements, the tool offers high flexibility in formula attribution. Alignment of mass spectra among different samples prior to molecular formula assignment improves mass error and facilitates molecular formula confirmation with the help of isotopologues. The online tool and the detailed instruction manual are freely accessible at www.icbm.de/icbm-ocean.

M-QAM signal transmission at the photonically generated K-band over thermal-induced turbulent FSO links with different turbulence distributions.

We present a theoretical and experimental study on the impact of different thermal-induced free-space turbulence distributions on the M-quadrature amplitude modulation (M-QAM) signal transmission in radio frequency K-band over hybrid optical links of standard single mode fiber (SSMF) and free-space optics (FSO). Frequency multiplication using an external intensity modulator biased at the null transmission point has been employed to photonically generate radio signals at a frequency of 25 GHz , included for the frequency bands for fifth-generation (5G) mobile networks. Moreover, extensive simulations have been performed for 10Gb/s with 4-, 16-, and 64-QAM over 5 km of SSMF and 500 m long FSO channels under scenarios with different turbulence levels and distributions. Proof-of-concept experiments have been conducted for 20 MHz with 4- and 64-QAM over 5 km of SSMF and 2 m long FSO channels under turbulence conditions. Both theoretical and experimental systems have been analyzed in terms of error vector magnitude (EVM) performance showing feasible transmission over the hybrid links in the received optical power range. Non-uniform turbulence distributions are shown to have a different impact on M-QAM modulation formats, i.e., turbulence distributions with higher strength in the middle of the FSO link reveal a 1.9 dB penalty when using 64-QAM signals compared to a 1.3 dB penalty using 4-QAM signals, whereas higher penalties have been measured when 4-QAM format is transmitted over turbulence distributions with larger magnitude in the second half of the FSO link. The results have been validated by theoretical predictions and lead to practical consequences on future networks' deployment.

Ammonia-oxidizing archaea release a suite of organic compounds potentially fueling prokaryotic heterotrophy in the ocean.

Ammonia-oxidizing archaea (AOA) constitute a considerable fraction of microbial biomass in the global ocean, comprising 20%-40% of the ocean's prokaryotic plankton. However, it remains enigmatic to what extent these chemolithoautotrophic archaea release dissolved organic carbon (DOC). A combination of targeted and untargeted metabolomics was used to characterize the exometabolomes of three model AOA strains of the Nitrosopumilus genus. Our results indicate that marine AOA exude a suite of organic compounds with potentially varying reactivities, dominated by nitrogen-containing compounds. A significant fraction of the released dissolved organic matter (DOM) consists of labile compounds, which typically limit prokaryotic heterotrophic activity in open ocean waters, including amino acids, thymidine and B vitamins. Amino acid release rates corresponded with ammonia oxidation activity and the three Nitrosopumilus strains predominantly released hydrophobic amino acids, potentially as a result of passive diffusion. Despite the low contribution of DOC released by AOA (~0.08%-1.05%) to the heterotrophic prokaryotic carbon demand, the release of physiologically relevant metabolites could be crucial for microbes that are auxotrophic for some of these compounds, including members of the globally abundant and ubiquitous SAR11 clade.

A New Phenotyping Pipeline Reveals Three Types of Lateral Roots and a Random Branching Pattern in Two Cereals.

Recent progress in root phenotyping has focused mainly on increasing throughput for genetic studies, while identifying root developmental patterns has been comparatively underexplored. We introduce a new phenotyping pipeline for producing high-quality spatiotemporal root system development data and identifying developmental patterns within these data. The SmartRoot image-analysis system and temporal and spatial statistical models were applied to two cereals, pearl millet (Pennisetum glaucum) and maize (Zea mays). Semi-Markov switching linear models were used to cluster lateral roots based on their growth rate profiles. These models revealed three types of lateral roots with similar characteristics in both species. The first type corresponds to fast and accelerating roots, the second to rapidly arrested roots, and the third to an intermediate type where roots cease elongation after a few days. These types of lateral roots were retrieved in different proportions in a maize mutant affected in auxin signaling, while the first most vigorous type was absent in maize plants exposed to severe shading. Moreover, the classification of growth rate profiles was mirrored by a ranking of anatomical traits in pearl millet. Potential dependencies in the succession of lateral root types along the primary root were then analyzed using variable-order Markov chains. The lateral root type was not influenced by the shootward neighbor root type or by the distance from this root. This random branching pattern of primary roots was remarkably conserved, despite the high variability of root systems in both species. Our phenotyping pipeline opens the door to exploring the genetic variability of lateral root developmental patterns.

M-QAM transmission over hybrid microwave photonic links at the K-band.

Two experimental configurations of a hybrid K-band (25 GHz) microwave photonic link (MPL) are investigated for seamless broadband wireless access networks. Experimental configurations consist of optical fiber, free-space optics (FSO) and radio frequency (RF) wireless channels. We analyze in detail the effects of channel impairments, namely fiber chromatic dispersion, atmospheric turbulence and multipath-induced fading on the transmission performance. In the first configuration, transmission of the 64-quadrature amplitude modulation (QAM) signal with 5, 20 and 50 MHz bandwidths over 5 km standard single-mode fiber (SSMF), 2 m turbulent FSO and 3 m RF wireless channels is investigated. We show that, for QAM with a high bandwidth, the link performance is being affected more by atmospheric turbulence. In the second configuration, the 20 MHz 4/16/64-QAM signals over a 50 km SSMF and 40 m FSO/RF wireless links are successfully transmitted with the measured error vector magnitude (EVM) values of 12, 9 and 7.9%, respectively. It is shown that, for all transmitted microwave vector signals, the bit error rate is lower than the hard-decision forward-error-correction limit of 3.8×10-3. Moreover, an extended FSO link span of 500 m for 25 GHz hybrid MPL with 16-QAM at 10 Gb/s under the weak and strong turbulence regimes is evaluated via simulation analysis to mimic a practical outdoor system.

Inscription of Bragg gratings in undoped PMMA mPOF with Nd:YAG laser at 266†nm wavelength.

We present the first Bragg gratings fabricated in two, three and five rings undoped PMMA microstructured polymer optical fibres (mPOFs) with relative low cost 266 nm Nd:YAG laser in the 850 nm region. The fibers were connectorised with commercial ferrules for easy coupling with silica patch cables. Temperature, humidity and strain sensitivities are measured and also the impact of ring structure and the diameter of POF on the characterization measurements are studied for potential applications. We also analyzed the effect of the number of hexagonal rings structure in gratings fabrication, noticing that larger number of rings lead to more difficulties to obtain strong gratings, where we consider this performance due to the scattering effects. We demonstrate Bragg gratings fabrication in 5-rings structure mPOF after 6 min by using 266 nm Nd:YAG laser whereas no Bragg gratings have been fabricated so far using 325 nm He-Cd laser system. Up to 30 dB relative reflected power gratings are obtained in two rings mPOF, showing good time stability and promising results for undoped mPOF applications.

Fabrication of tunable chirped mPOF Bragg gratings using a uniform phase mask.

We demonstrate chirped Bragg gratings fabrication in doped microstructured tapered polymer fibers by using a uniform phase mask. The use of high photosensitive benzyl dimethyl ketal (BDK) doped core fiber allows to obtain chirped Bragg gratings by means of a single krypton fluoride laser pulse. The stability of the gratings has been confirmed and the strain and temperature sensitivity measurements demonstrate their tunable properties. Finally, different tapered profiles have been implemented in order to show the potentiality of this fabrication technique in polymer optical fibers.

Polymer optical fiber Bragg grating inscription with a single Nd:YAG laser pulse.

We experimentally demonstrate the first polymer optical fiber Bragg gratings inscribed with only one Nd:YAG laser (266 nm) pulse. The gratings have been inscribed in a single-mode poly (methyl methacrylate) optical fiber, with a core doped with benzyl dimethyl ketal for photosensitivity enhancement. One laser pulse with a duration of 8 ns and energy of 72 µJ is adequate to introduce a refractive index change of 0.5 × 10-4 in the fiber core. The stability of the gratings has been confirmed and the strain and temperature sensitivity measurements demonstrate their tunable properties.

Hot water-assisted fabrication of chirped polymer optical fiber Bragg gratings.

We obtained chirped gratings by performing hot water gradient thermal annealing of uniform poly (methylmethacrylate) (PMMA) microstructured polymer optical fiber Bragg gratings (POFBGs). The proposed method's simplicity is one of its main advantages because no special phase mask or additional etching are needed. It not only enables easy control tuning of the central wavelength and chirp characteristics, but it also leads to obtain flexible grating response, compared with tapered chirped POFBGs. Therefore, a flexible and low-cost chirped POFBG devices fabrication technique has been presented by using a single uniform phase mask.

Largely tunable dispersion chirped polymer FBG.

We demonstrate a largely tunable dispersion fiber Bragg grating (FBG) inscribed in a microstructured polymer optical fiber (mPOF). The bandwidth of the chirped FBG (CFBG) was achieved from 0.11 to 4.86 nm, which corresponds to a tunable dispersion range from 513.6 to 11.15 ps/nm. Furthermore, thermal sensitivity is used to compensate for the wavelength shift due to the applied strain. These results demonstrate that a CFBG in a POF is a promising technology for future optical systems.

Experimental photonic generation of chirped pulses using nonlinear dispersion-based incoherent processing.

We experimentally demonstrate, for the first time, a chirped microwave pulses generator based on the processing of an incoherent optical signal by means of a nonlinear dispersive element. Different capabilities have been demonstrated such as the control of the time-bandwidth product and the frequency tuning increasing the flexibility of the generated waveform compared to coherent techniques. Moreover, the use of differential detection improves considerably the limitation over the signal-to-noise ratio related to incoherent processing.

Direct and indirect childhood victimization and their influence on the development of adolescents antisocial behaviors.

OBJECTIVE: Experiences of direct and indirect victimization can be very common among children and adolescents of the general population and can have an important impact on their behavior. The general aim of this study was to analyze the possible association between childhood victimization and the development of antisocial behaviors during adolescence. The prevalence of child victimization and antisocial behaviors self-reported by adolescents is shown. Therefore, the individual associations between different types of childhood victimization and antisocial behaviors were explored. METHOD: The sample comprised 459 general population adolescents. Data on six types of childhood victimization were collected using the Spanish version of the Juvenile Victimization Questionnaire, and information on five types of antisocial behaviors was gathered using the short version of the Antisocial Behavior Questionnaire. RESULTS: More than 90% of the adolescents had been victimized at least once, while almost half of them had engaged in at least one type of antisocial behavior in the last 12 months. Witnessing and indirect victimization is the only type of childhood victimization associated with the antisocial behaviors analyzed. CONCLUSIONS: These results corroborate previous findings suggesting that witnessing and indirect victimization may have the same or similar impact to that of direct victimization on adolescents' social adjustment. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

High-order UWB pulses scheme to generate multilevel modulation formats based on incoherent optical sources.

We present a high-order UWB pulses generator based on a microwave photonic filter which provides a set of positive and negative samples by using the slicing of an incoherent optical source and the phase inversion in a Mach-Zehnder modulator. The simple scalability and high reconfigurability of the system permit a better accomplishment of the FCC requirements. Moreover, the proposed scheme permits an easy adaptation to pulse amplitude modulation, bi phase modulation, pulse shape modulation and pulse position modulation. The flexibility of the scheme for being adaptable to multilevel modulation formats permits to increase the transmission bit rate by using hybrid modulation formats.

The relationship between social media addiction, fear of missing out and family functioning: a structural equation mediation model.

BACKGROUND: The use of social media became a daily activity for many individuals, with recreational, informational, and social purposes, to name a few. However, for some subjects, the use of these platforms may become problematic and generate functioning impairments in many life areas. Given this, the present research aimed at investigating the factor that may contribute to Social Media Addiction, by focusing on Fear of Missing Out and Family Functioning Patterns. METHODS: A sample of 303 social media users (Mage = 35.29; SD = 14.87; 65% females, 35% males) completed a survey including the Bergen Social Media Addiction Scale, Family Adaptability and Cohesion Evaluation Scales-IV, and Fear of Missing Out Scale. Data were analysed by implementing Pearson correlation and testing a mediation with the Structural Equation Model approach. RESULTS: Cohesion, flexibility, and disengagement family functioning patterns were significantly associated with the levels of social media addiction. These dimensions were inserted in the structural equation model, where the full mediation of fear of missing out in their relationship with social media addiction was shown. CONCLUSIONS: The data showed the protective effect of flexible and cohesive family functioning patterns, as well as the role of disengagement and, sequentially, fear of missing out as risk factors. These findings may provide useful indications to elaborate tailored and effective therapeutic and preventive activity.

Wearable cardiorespiratory monitoring with stretchable elastomer optical fiber.

This work presents a stretchable elastomer optical fiber sensor incorporated into a belt for respiratory rate (RR) and heart rate (HR) monitoring. Different materials and shapes of prototypes designed were tested in terms of performance and the best choice was identified. The optimal sensor was tested by 10 volunteers to evaluate the performance. The proposed elastomer optical fiber sensor can achieve simultaneous measurement of RR and HR in different body positions, and also ballistocardiography (BCG) signal measurement in the lying position. The sensor has good accuracy and stability, with maximum errors of 1 bpm and 3 bpm for RR and HR, respectively, and average weighted mean absolute percentage error (MAPE) of 5.25% and root mean square error (RMSE) of 1.28 bpm. Moreover, the results of the Bland-Altman method showed good agreement of the sensor with manual counting of RR and with electrocardiogram (ECG) measurements of HR.

Nonlinear dispersion-based incoherent photonic processing for microwave pulse generation with full reconfigurability.

A novel all-optical technique based on the incoherent processing of optical signals using high-order dispersive elements is analyzed for microwave arbitrary pulse generation. We show an approach which allows a full reconfigurability of a pulse in terms of chirp, envelope and central frequency by the proper control of the second-order dispersion and the incoherent optical source power distribution, achieving large values of time-bandwidth product.

Highly chirped single-bandpass microwave photonic filter with reconfiguration capabilities.

We propose a novel photonic structure to implement a chirped single-bandpass microwave photonic filter based on the amplitude modulation of a broadband optical signal transmitted by a non-linear dispersive element and an interferometric system prior to balanced photodetection. A full reconfigurability of the filter is achieved since amplitude and phase responses can be independently controlled. We have experimentally demonstrated chirp values up to tens of ns/GHz, which is, as far as we know, one order of magnitude better than others achieved by electrical approaches and furthermore, without restrictions in terms of frequency tuning since a frequency operation range up to 40 GHz has been experimentally demonstrated.

Centralized light-source optical access network based on polarization multiplexing.

This paper presents and demonstrates a centralized light source optical access network based on optical polarization multiplexing technique. By using two optical sources emitting light orthogonally polarized in the Central Node for downstream and upstream operations, the Remote Node is kept source-free. EVM values below telecommunication standard requirements have been measured experimentally when bidirectional digital signals have been transmitted over 10 km of SMF employing subcarrier multiplexing technique in the electrical domain.