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J Steroid Biochem Mol Biol ; 78(5): 481-92, 2001 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11738558

RESUMEN

Our aim was to investigate the effect of a single testosterone (T) injection on the androgen receptor (AR) in rat skeletal muscle (SM) cytosol. The properties of AR were studied in order to establish the protocol for differential determination of free and hormone-occupied AR in SM cytosols from non-hormone-deficient animals. Using the developed ligand-exchange protocol, we demonstrated that injection of T (1 mg/kg) caused alternating changes of the total AR binding. The binding minimum (23% of the control) was measured 1 h after the injection. It was followed by pronounced and lasting elevation of the AR binding. In the control cytosols, AR complexes constituted approximately 25% of the total receptor content. Changes of their relative content immediately after T administration were consistent with rapid nuclear translocation of the AR. Inhibition of protein synthesis by cycloheximide (CHI) injection demonstrated that delayed and lasting increase of the AR binding after T injection partially depended on the stimulated protein synthesis. Altogether, the obtained evidence supports the assumption that the AR mediates elevation of its own gene expression in SM upon administration of T.


Asunto(s)
Músculo Esquelético/efectos de los fármacos , Músculo Esquelético/metabolismo , Receptores Androgénicos/efectos de los fármacos , Receptores Androgénicos/metabolismo , Testosterona/farmacología , Animales , Cicloheximida/farmacología , Citosol/metabolismo , Cinética , Masculino , Inhibidores de Proteasas/farmacología , Inhibidores de la Síntesis de la Proteína/farmacología , Ratas , Testosterona/metabolismo , Compuestos de Tosilo/farmacología
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