RESUMEN
The diagnostic value of preparations is commonly characterized by sensitivity and specificity. But not all these characteristics make it possible to decide unequivocally which of the preparations to be compared is superior to the other one with respect to its diagnostic value. It is proposed that in the choice of a diagnostic preparation its capacity to provide data for exact diagnosis should be considered, i.e. the additional characteristic indicating the spread of the disease under study. As an example, the comparison of the diagnostic value of conventional methods and the polymerase chain reaction in the diagnostics of helicobacteriosis is presented. The described method for the evaluation of the diagnostic value of the preparation is well-grounded, simple and obvious.
Asunto(s)
Infecciones por Helicobacter/diagnóstico , Helicobacter pylori/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Juego de Reactivos para Diagnóstico , Antígenos Bacterianos/genética , Proteínas Bacterianas/genética , Cartilla de ADN , Genes Bacterianos , Helicobacter pylori/genética , Humanos , Sensibilidad y EspecificidadRESUMEN
Pyocinovar and serovar characteristics of 91 P.aeruginosa strains isolated from patients and the environment in a hospital for premature children, a child survey hospital and a neonatological hospital. The leading epidemiological markers of P.aeruginosa strains, among them pyocinovar 883722, serovar 6 (a hospital for premature children), pyocinovar 888888, serovar 4 (a child surgery hospital), pyocinovars 888888 and 861322 (a neonatological hospital).
Asunto(s)
Infección Hospitalaria/microbiología , Microbiología Ambiental , Infecciones por Pseudomonas/microbiología , Pseudomonas aeruginosa/aislamiento & purificación , Adulto , Biomarcadores , Niño , Infección Hospitalaria/epidemiología , Humanos , Recién Nacido , Recien Nacido Prematuro , Infecciones por Pseudomonas/epidemiología , Pseudomonas aeruginosa/clasificación , Piocinas/clasificación , Federación de Rusia/epidemiología , SerotipificaciónRESUMEN
Commercial system of multimicrotests for biochemical identification of enterobacteria MMT E2 and routine tube tests were compared in a controlled epidemiologic experiment with 205 enterobacterial strains. The system has been developed at the Allergen Research and Production Amalgamation. System MMT E2 fairly well met the requirements to diagnostic preparations of this kind; its results were compatible and well reproducible. This system in complex with commercial MMT E1 system may be used for species identification of enterobacteria. Practical application of both the systems will considerably simplify the process of identification of these microorganisms.
Asunto(s)
Técnicas Bacteriológicas , Infecciones por Enterobacteriaceae/diagnóstico , Enterobacteriaceae/aislamiento & purificación , Citrobacter/aislamiento & purificación , Diagnóstico Diferencial , Enterobacter/aislamiento & purificación , Infecciones por Enterobacteriaceae/prevención & control , Escherichia/aislamiento & purificación , Estudios de Evaluación como Asunto , Klebsiella/aislamiento & purificación , Proteus/aislamiento & purificación , Providencia/aislamiento & purificación , Salmonella/aislamiento & purificación , Shigella/aislamiento & purificación , Yersinia/aislamiento & purificaciónRESUMEN
Investigation of 108 P. aeruginosa strains isolated from various clinical material revealed that 54.63% of them belonged to serogroups 2, 5, 16, 18, 20 and 12.96% to serotype 4. Five strains were polyagglutinated. Monocultures of P. aeruginosa were isolated from 55% of patients, in the majority of cases (31 out of 36) these strains were isolated from the urine. Bacterial associations were more frequently detected in discharge from wounds (11 out of 21), in the material obtained by bronchoscopy (9 out of 15), and in the throat (5 out of 6). Assessment of antibioticograms of the isolated P. aeruginosa strains permitted us characterize polymixin, forte ceftazidime, and biseptol as effective agents.
Asunto(s)
Pseudomonas aeruginosa/aislamiento & purificación , Antibacterianos/farmacología , Broncoscopía , Humanos , Pruebas de Sensibilidad Microbiana , Pseudomonas aeruginosa/clasificación , Pseudomonas aeruginosa/efectos de los fármacos , Orina/microbiología , Heridas y Lesiones/microbiologíaRESUMEN
Comparative evaluation of a system of multimicrotests for biochemical identification of enterobacteria (MMT E1) developed at the ALLERGEN Research and Production Amalgamation and of tube tests for the same purpose in a controlled epidemiologic experiment has shown that the suggested system meets the requirements to such diagnostic preparations by all the major qualitative parameters (compatibility and reproducibility of the test results). Practical use of MMT E1 system will simplify and facilitate the process of enterobacteria identification.
Asunto(s)
Técnicas Bacteriológicas , Enterobacteriaceae/aislamiento & purificación , Infecciones por Enterobacteriaceae/diagnóstico , Infecciones por Enterobacteriaceae/epidemiología , Estudios de Evaluación como AsuntoAsunto(s)
Infecciones por Bordetella/diagnóstico , Bordetella pertussis/genética , Bordetella/genética , ADN Bacteriano/análisis , Infecciones Estreptocócicas/diagnóstico , Streptococcus/genética , Tos Ferina/diagnóstico , Adulto , Niño , Diagnóstico Diferencial , Humanos , Lactante , Recién Nacido , Reacción en Cadena de la PolimerasaRESUMEN
Experiments with 39 type collection strains and 25 newly isolated strains of P. aeruginosa have demonstrated the possibility of preserving the culture viability for a long time in sterile tap water at 6-8 degrees C. The initial morphologic, cultural, biochemical, and antigenic characteristics of the cultures are unchanged in sealed ampules for up to 1 year and in glass tubes corked with rubber corks for up to 8 months.
Asunto(s)
Pseudomonas aeruginosa/crecimiento & desarrollo , Técnicas BacteriológicasRESUMEN
Examinations of Pseudomonas aeruginosa strains isolated from clinical material from newborns and puerperae and from the environmental objects (washings off, water) have shown that 45.45% of these strains belonged to serotype 04, 24.55% to serotype 02, and 16.38% to other serotypes. Serotype 04 was the most incident in all the tested samples, it was detectable in newborns' biotopes and on environmental objects. Serotype 02 strains ranked second in incidence; they were detected in clinical material from the newborns but not on environmental objects.