[On efficient in vitro purification of cell suspensions containing malignant cells].

We propose a method for magnetic sorting of cell suspensions able to differentiate not only cells with single specific antigen on their surface, but also cells with a group of pre-defined antigens. Individually, each antigen of this group may be present on surfaces of non-selected cells. However, only the simultaneous presence of all given antigens on the cell surface means that such a cell should be separated. The method is of interest, for purification of cell suspensions from malignant cells, in particular, for purification of bone marrow material for autologous transplantation in leukemia.

[Correlations between clusters of protein-DNA binding sites and the binding experimental data allow to predict a structure of regulatory modules].

Here we analyzed an option to predict the structure of cis-regulatory modules that consist of binding sites of different proteins (heterogeneous cis-regulatory modules) using mutual positional correlations between protein-DNA binding experimental data and computationally identified clusters of binding sites for each of the proteins.

[Splice sites are overrepresented in Pasilla binding motif clusters in D. melanogaster genes].

For RNA-binding protein Pasilla, which has been shown to play a role in alternative splicing regulation, binding sites and clusters of binding sites are found in silico in the whole genome of D. melanogaster. The current study analyzes the occurrence of splice sites in binding site clusters. Several hundred thousand binding site motifs and thousands of significant motif clusters were identified. It was discovered that exon-intron borders in D. melanogaster genes are reliably found within Pasilla binding motif clusters, with a higher frequency than could be otherwise expected based on a random model. Additionally, donor splice sites are found in Pasilla clusters twice as often as acceptor sites. This phenomena is observed both for exons annotated as alternatively spliced and for exons annotated as constitutive. These observations support the hypothesis that Pasilla plays a functional role in splicing regulation of D. melanogaster.

[A comparative analysis of the binding sites of proteins regulating the transcription in the early development of Drosophila melanogaster, determined by the ChIP-chip method and the theoretically predicted clusters of the binding sites of these proteins].

DNA segments were analyzed for six proteins regulating the transcription in the early embryonic development of Drosophila melanogaster in which a significant binding of these proteins was observed by the method of immunoprecipitation of chromatin followed by the precipitation on the template (Chip-chip). The regions of high density of the theoretically predicted binding sites of these proteins in these fragments were considered. A comparison of the experimental and theoretical data for various pairwise combinations of the above-mentioned factors was made. It was shown that the intensity of data obtained by the ChiP-chip method significantly differs outside and inside identified clusters. A comparison of experimental data in various pairwise combinations of the factors showed that, despite the problems associated with a great noise in the ChiP-chip signal, a strong tendency of particular groups of factors to bind in the same DNA regions was revealed, which is consistent with the data about their functional coupling. Thus, a comparison of the data obtained by the ChiP-chip method makes it possible to distinguish functionally related regulatory subsystems even in the case of systems that are much less investigated than the system of the early embryonic development of Drosophila melanogaster.

[Clinical aspects and diagnostic characteristics of nontraumatic intracerebral hemorrhage]. / Klinika ta osoblyvosti diahnostyky netravmatychnoho vnutrishn'omozkovoho krovovylyvu.

The results of treatment of 235 young and middle age patients with nontraumatic intracerebral haemorrhage were analyzed. Diagnosis was verified with the help of computer tomography, magnetic-resonance tomography, angiography and basing on operational and autopsy data as well. Three main symptoms, reflecting the dislocational changes dynamics, were assigned.