Sequential G-banding and fluorescent in situ hybridization on peripheral blood, bone marrow, and amniotic fluid samples.

We describe a method for fluorescent in situ hybridization (FISH) on previously G-banded slides and mounted coverslip preparations. The technique is successful on previously G-banded slides from peripheral blood and bone marrow specimens and on amniotic fluid samples from in situ harvests. Satellite sequence probes, whole chromosome paints, and unique sequence probes have all produced reliable results.

Peripheral blood erythroid progenitors from patients with sickle cell anemia: HPLC separation of hemoglobins and the effect of a HbF switching factor.

We describe the use of HPLC for the separation and quantitation of hemoglobin and globin chains. Utilization of this sensitive technique allows the analysis of hemoglobin and globin chains in the total accumulated and newly synthesized hemoglobin. Using this methodology, we have studied the effect of a previously described HbF factor in fetal calf serum on peripheral blood BFUe-derived erythroblasts from patients with sickle cell anemia. As in previous studies of peripheral blood BFUe-derived erythroblasts from hematologically normal adults, this HbF factor promoted the increased synthesis of HbF in the BFUe-derived erythroblasts from the sickle cell anemia patients.