Interkingdom ecological networks between plants and fungi drive soil multifunctionality across arid inland river basin.

Despite the known collective contribution of above- (plants) and below-ground (soil fungi) biodiversity on multiple soil functions, how the associations among plant and fungal communities regulate soil multifunctionality (SMF) differentially remains unknown. Here, plant communities were investigated at 81 plots across a typical arid inland river basin, within which associated soil fungal communities and seven soil functions (nutrients storage and biological activity) were measured in surface (0-15 cm) and subsurface soil (15-30 cm). We evaluated the relative importance of species richness and biotic associations (reflected by network complexity) on SMF. Our results demonstrated that plant species richness and plant-fungus network complexity promoted SMF in surface and subsurface soil. SMF in two soil layers was mainly determined by plant-fungus network complexity, mean groundwater depth and soil variables, among which plant-fungus network complexity played a crucial role. Plant-fungus network complexity had stronger effects on SMF in surface soil than in subsurface soil. We present evidence that plant-fungus network complexity surpassed plant-fungal species richness in determining SMF in surface and subsurface soil. Moreover, plant-fungal species richness could not directly affect SMF. Greater plant-fungal species richness indirectly promoted SMF since they ensured greater plant-fungal associations. Collectively, we concluded that interkingdom networks between plants and fungi drive SMF even in different soil layers. Our findings enhanced our knowledge of the underlying mechanisms that above- and below-ground associations promote SMF in arid inland river basins. Future study should place more emphasis on the associations among plant and microbial communities in protecting soil functions under global changes.

Groundwater depth regulates assembly processes of abundant and rare bacterial communities across arid inland river basin.

Although numerous studies on bacterial biogeographic patterns in dryland have been conducted, bacterial community assembly across arid inland river basins is unclear. Here, we assessed the ecological drivers that regulate the assembly processes of abundant (ABS) and rare (RBS) bacterial subcommunities based on 162 soil samples collected in an arid inland river basin of China. The results showed that: (1) ABS exhibited a steeper distance-decay slope, and were more strongly affected by dispersal limitation (75.5% and 84.5%), than RBS in surface and subsurface soil. RBS were predominantly controlled by variable selection (54.6% and 50.2%). (2) Soil electric conductivity played a decisive role in mediating the balance between deterministic and stochastic processes of ABS and RBS in surface soil, increasing soil electric conductivity increased the importance of deterministic process. For subsurface soil, soil available phosphorus (SAP) and soil pH drove the balance in the assembly processes of ABS and RBS, respectively. The RBS shifted from determinism to stochasticity with decreased pH, while the dominance of deterministic processes was higher in low-SAP sites. (3) Groundwater depth seasonality had substantial effects on the assembly processes of ABS and RBS, but groundwater depth seasonality affected them indirectly mainly by regulating soil properties. Collectively, our study provides robust evidence that groundwater-driven variations in soil properties mediates the community assembly process of soil bacteria in arid inland river basins. This finding is of importance for forecasting the dynamics of soil microbial community and soil process in response to current and future depleted groundwater.

Root attributes dominate the community assembly of soil fungal functional guilds across arid inland river basin.

Plant attributes are increasingly acknowledged as key drivers shaping soil fungal communities, but considerable uncertainty exists over fungal community assembly mechanisms and their plant drivers based only on inferences from plant aboveground attributes. To date, empirical evidences of how root attributes are integrated into microbiome-plant linkages remain limited. Using 162 soil samples from a typical arid inland river basin in China, we assessed the drivers that regulate the distribution patterns and assembly processes of total, mycorrhizal, saprotrophic and pathotrophic fungi in surface (0-15 cm) and subsurface soils (15-30 cm). Total fungi and fungal functional guilds exhibited similar distribution patterns in arid inland river basins. Null-model and variance partitioning analysis revealed that the heterogeneous selection induced by root attributes, rather than dispersal limitation, predominated the fungal community assembly. Multiple regressions on matrices further demonstrated that specific root length were the most important predictors of fungal community assembly, which mediated the balance of assembly processes of soil fungal communities. Heterogeneous selection decreased for total, mycorrhizal and saprotrophic fungi, but increased for pathotrophic fungi with increasing specific root length. Additionally, fine-root biomass exerted important effects on fungal assembly processes in subsurface soil but not in surface soil, suggesting root attributes differently affected fungal community assembly between surface and subsurface soil. Collectively, our study highlights the importance of considering root attributes in differentiating the balance of stochastic and deterministic processes in microbial community assembly.

Dispersal limitation dominates the community assembly of abundant and rare fungi in dryland montane forests.

The assembly mechanisms and drivers of abundant and rare fungi in dryland montane forest soils remain underexplored. Therefore, in this study, we compared the distribution patterns of abundant and rare fungi and explored the factors determining their assembly processes in a dryland montane forest in China. Stronger distance-decay relationships (DDRs) were found in abundant sub-communities than in rare sub-communities. In addition, abundant fungi exhibited greater presence and wider habitat niche breadth than rare fungi. Both the null model and variation partitioning analysis indicated that dispersal limitation and environmental selection work together to govern both abundant and rare fungal assembly, while dispersal limitation plays a dominant role. Meanwhile, the relative influence of dispersal limitation and environmental selection varied between abundant and rare sub-communities, where dispersal limitation showed greater dominance in abundant fungal assembly. Mantel tests demonstrated that soil pH and phosphorus played critical roles in mediating abundant and rare fungi assembly processes, respectively. Our findings highlight that the distinct biogeographic patterns of abundant and rare fungi are driven by different assembly mechanisms, and the assembly processes of abundant and rare fungi are determined by diverse ecological drivers in dryland montane forest soils.

Plant traits and community composition drive the assembly processes of abundant and rare fungi across deserts.

The difference in community assembly mechanisms between rare and abundant fungi in deserts remains unknown. Hence, we compared the distribution patterns of abundant and rare fungi, and assessed the factors driving their assembly mechanisms across major vegetation types (shrubby desert, semi-shrubby and dwarf semi-shrubby desert, dwarf semi-arboreous desert, and shrubby steppe desert) of Chinese deserts. We assessed abundant and rare fungal subcommunities base on the sequencing data of fungal ITS data. Abundant fungal assembly was more affected by neutral processes than the rare. Null model and VPA analysis indicated that heterogeneous selection dominated rare sub-communities, whereas abundant fungal assembly was mainly determined by heterogeneous selection, dispersal limitation and other, unknown processes together. As a result, abundant sub-communities exhibited a higher species turnover rate than the rare. Hierarchical partitioning analysis indicated that soil conditions and plant attributes drove the assembly processes of abundant and rare fungi, respectively. Meanwhile, the relative strength of different assembly processes differed significantly among four vegetation types. In addition, we found that plant functional traits and composition played more critical roles in shaping the assembly processes of rare fungi than those of abundant fungi. Taken together, our findings collectively suggest that rare and abundant fungi exhibit differential ecological patterns that are driven by distinct assembly processes in deserts. We emphasize that the assembly processes of abundant and rare fungi are dependent on different abiotic and biotic factors in desert ecosystems.

Testing the Functional and Phylogenetic Assembly of Plant Communities in Gobi Deserts of Northern Qinghai-Tibet Plateau.

The mechanism governing plant community assembly across large-scale Gobi deserts remains unclear. Here, we inferred the roles of different assembly processes in structuring plant communities in the Gobi deserts of the Qinghai-Tibet Plateau by using a phylogenetic tree, and leaf and root traits. The functional and phylogenetic structures of 183 plant communities were assessed, and their distributions were linked with environmental gradients. Our results demonstrated that functional convergence was prevalent in most functional traits (75% of the traits) and accentuated when all traits were combined. The phylogenetic structure exhibited significant divergence. We observed the contrasting response of functional and phylogenetic assembly structures to environmental gradients. More importantly, we found that the shifts in the functional assembly along environmental gradients were trait-specific, with dominant roles of local factors, such as gravel coverage and soil attributes, in determining the distribution patterns of most traits. However, the distribution patterns of leaf P concentration (LPC), root N concentration (RNC), and root P concentration (RPC) were mainly driven by climatic factors. These results reveal that niche-based processes, such as abiotic filtering and weaker competitive exclusion, are the major drivers of species co-occurrence, which results in the widespread coexistence of phylogenetically distinct but functionally similar species within the Gobi plant community. Our findings could improve the understanding of plant community assembly processes and biodiversity maintenance in extremely harsh drylands.