RESUMEN
Organized tissue fragments obtained by dissociation of a pure mucoid carcinoma of the breast were cultured in vitro. The cellular organization of the fragments appears similar to that observed in vivo, and consist of mucous-filled spherules surrounded by a single layer of tumour cells. Time-lapse video recordings revealed that the cells surrounding these fragments undergo a concerted contraction and relaxation over the time span of several hours. The presence of cytokeratin and oestrogen receptors in the cells suggests an epithelial derivation. Movement of tumour fragments of the type described here could potentially influence the metastatic process of some breast cancers.
Asunto(s)
Adenocarcinoma Mucinoso/patología , Neoplasias de la Mama/patología , Femenino , Humanos , Células Tumorales CultivadasRESUMEN
In the present study, we evaluated the seroprevalence of HTLV-I/II infection among the blood donors in Santa Fe and Santiago del Estero provinces. A total of 1327 serum samples from blood donors from Rafaela blood bank of Santa Fe province and 3382 serum samples from blood donors from Dr Edgar Bouzon blood bank of Santiago del Estero province were studied. The antibody screening was done by particle agglutination assay (PA) (SERODIA, Fujirebio Inc., Tokyo, Japan) or by enzyme immuno assay (EIA) (Abbott HTLV-I/HTLV-II EIA, Abbott, Germany). The "in house" indirect immunofluorescence assay (IFA) and Western blot (Bioblot HTLV Biokit, Barcelona, Spain) were used as confirmatory assays. All the samples resulted negative for specific antibodies against HTLV-I/II. These results suggest that HTLV-I/II are not circulating in low risk populations in these provinces or that the prevalences of infection would be lower than that reported by blood banks in other provinces of Argentina.
Asunto(s)
Donantes de Sangre , Infecciones por HTLV-I/epidemiología , Infecciones por HTLV-II/epidemiología , Adulto , Argentina/epidemiología , Bancos de Sangre , Femenino , Anticuerpos Anti-HTLV-I/sangre , Anticuerpos Anti-HTLV-II/sangre , Humanos , Masculino , Prevalencia , Riesgo , Estudios SeroepidemiológicosAsunto(s)
Prótesis Valvulares Cardíacas , Computadores , Humanos , Técnicas In Vitro , Matemática , Válvula Mitral , Diseño de PrótesisRESUMEN
Spurious background fringes have been observed in time-resolved holograms recorded with a pulsed laser source and a rapidly deflected reference beam. These fringes were determined to be a time smear effect due to the small change in the angle of incidence of the reference beam during the exposure time. By analyzing the phenomenon theoretically, equations were derived that could be used to describe the observed fringe formation. A numerical solution was obtained for a realistic laser pulse time profile and an analytical solution was obtained using a simple mathematical model of the pulse profile. These solutions allow an a priori determination of bounds of the exposure parameters that would reduce the time smear effects to acceptable levels.
RESUMEN
An apparatus has been designed to retain the spatial orientation of breast biopsies throughout surgery, transport and processing in the pathology laboratory. It does not interfere with regular surgical procedures and simplifies the surgeon's task of marking the orientation of a specimen. As the use of the apparatus does not involve either chemical or physical substances it is an appropriate handling procedure for those specimens requiring sensitive testing procedures such as cell culture.
Asunto(s)
Biopsia/instrumentación , Neoplasias de la Mama/patología , Humanos , Manejo de Especímenes/instrumentaciónRESUMEN
In the present study, we evaluated the seroprevalence of HTLV-I/II infection among the blood donors in Santa Fe and Santiago del Estero provinces. A total of 1327 serum samples from blood donors from Rafaela blood bank of Santa Fe province and 3382 serum samples from blood donors from Dr Edgar Bouzon blood bank of Santiago del Estero province were studied. The antibody screening was done by particle agglutination assay (PA) (SERODIA, Fujirebio Inc., Tokyo, Japan) or by enzyme immuno assay (EIA) (Abbott HTLV-I/HTLV-II EIA, Abbott, Germany). The [quot ]in house[quot ] indirect immunofluorescence assay (IFA) and Western blot (Bioblot HTLV Biokit, Barcelona, Spain) were used as confirmatory assays. All the samples resulted negative for specific antibodies against HTLV-I/II. These results suggest that HTLV-I/II are not circulating in low risk populations in these provinces or that the prevalences of infection would be lower than that reported by blood banks in other provinces of Argentina.
RESUMEN
In the present study, we evaluated the seroprevalence of HTLV-I/II infection among the blood donors in Santa Fe and Santiago del Estero provinces. A total of 1327 serum samples from blood donors from Rafaela blood bank of Santa Fe province and 3382 serum samples from blood donors from Dr Edgar Bouzon blood bank of Santiago del Estero province were studied. The antibody screening was done by particle agglutination assay (PA) (SERODIA, Fujirebio Inc., Tokyo, Japan) or by enzyme immuno assay (EIA) (Abbott HTLV-I/HTLV-II EIA, Abbott, Germany). The "in house" indirect immunofluorescence assay (IFA) and Western blot (Bioblot HTLV Biokit, Barcelona, Spain) were used as confirmatory assays. All the samples resulted negative for specific antibodies against HTLV-I/II. These results suggest that HTLV-I/II are not circulating in low risk populations in these provinces or that the prevalences of infection would be lower than that reported by blood banks in other provinces of Argentina.(AU)
Asunto(s)
Adulto , Femenino , Humanos , Masculino , Donantes de Sangre , Infecciones por HTLV-I/epidemiología , Infecciones por HTLV-II/epidemiología , Argentina/epidemiología , Bancos de Sangre , Anticuerpos Anti-HTLV-I/sangre , Anticuerpos Anti-HTLV-II/sangre , Prevalencia , Riesgo , Estudios SeroepidemiológicosRESUMEN
In the present study, we evaluated the seroprevalence of HTLV-I/II infection among the blood donors in Santa Fe and Santiago del Estero provinces. A total of 1327 serum samples from blood donors from Rafaela blood bank of Santa Fe province and 3382 serum samples from blood donors from Dr Edgar Bouzon blood bank of Santiago del Estero province were studied. The antibody screening was done by particle agglutination assay (PA) (SERODIA, Fujirebio Inc., Tokyo, Japan) or by enzyme immuno assay (EIA) (Abbott HTLV-I/HTLV-II EIA, Abbott, Germany). The "in house" indirect immunofluorescence assay (IFA) and Western blot (Bioblot HTLV Biokit, Barcelona, Spain) were used as confirmatory assays. All the samples resulted negative for specific antibodies against HTLV-I/II. These results suggest that HTLV-I/II are not circulating in low risk populations in these provinces or that the prevalences of infection would be lower than that reported by blood banks in other provinces of Argentina.