Effect of raw and repeatedly fried mustard oil intake on metabolic and organ histological changes in Wistar rat.

Fried dietary oils often cause adverse health effects due to altered chemical and nutritional properties. In the present study with mustard oil, the effect of repeated frying (180°C for 10 min, three times) on chemical characteristics and oral intake of raw and fried mustard oil (5 g/kg for 5 weeks) in Wistar rats were investigated. Repeated frying caused oxidation reactions and free fatty acid content in mustard oil. This fried oil feeding to rats increased serum LDL cholesterol and triglycerides and decreased HDL leading to nonalcoholic fatty liver disease accounted by significant increment of liver enzymes (ALT, AST, ALP) compared with raw oil-fed group. Higher level of serum CK-MB and creatinine in fried oil-fed rats indicate disrupted kidney function. Histological findings of the respective organs ascertained metabolic abnormalities due to fried oil intake. Thus, the study suggests avoidance of using repeatedly fried oils in food products for better consumer health. PRACTICAL APPLICATIONS: Vegetable oil is a common ingredient of food products. Mustard oil is used in many countries both as raw and fried forms for various food preparations. When it is being fried at elevated temperatures in the presence of air, both thermal and oxidative decomposition take place which alter the nutritional and functional bioactive properties of the oil. The findings of the current study revealed the repeatedly fried mustard oil-induced detrimental effects on various organs of Wistar rats. Human beings consuming repeatedly fried mustard oil regularly might also face these acute problems. Thus in context to the public health issue, it is a message to avoid in taking repeated fried oils at household cooking or food industry to maintain better health.

Remedial role of exercise training to deep-fried oil-induced metabolic and histological changes in Wistar rats.

Exercise training is a well-known lifestyle to maintain good health. The present study was conducted to explore the effect of regular exercise training (for 15 min) on biochemical, physiological, and histological changes in fried oil intake (5 g/kg body weight, for 5 weeks) with or without raw oil supplementation. Liver disease and heart muscle injury were accounted for by significant (p < .05) increase in liver and heart biomarker enzymes in serum. Creatinine and urea level were also significantly increased in the fried oil-fed group as a sign of kidney injury. But all the biological markers including triglycerides, low-density lipoprotein cholesterol (LDL-C) was decreased significantly (p < .05) in the exercise-trained rat group. These metabolic changes were substantiated by the histological study of respective organs. Therefore, people should avoid repeatedly deep-fried oil consumption rather than fresh ones though regular exercise training has been found to resolve these metabolic abnormalities. PRACTICAL APPLICATIONS: Exercise training is a common practice to reduce the onset of some metabolic abnormalities for example cardiovascular disease, non-alcoholic fatty liver disease, chronic kidney disease, and also prevents various cell and tissue damages. Repeated frying of vegetable oil not only reduces its nutritional value but also produce free fatty acids and other toxic compounds. The present study revealed that repeatedly heated vegetable oil consumption causes injuries in the heart, liver, kidney, and small intestine by the oxidative products. As a result, the level of biomarkers of these responsive organs was found to be elevated. But regular exercise training ameliorated these detrimental effects. So, in terms of public health concern, regular exercise is one of the best ways to keep the body fit especially those who are consuming deep-fried oil.

tRNA diversification among uncultured archeon clones.

Whole genome sequences (DNA sequences) of four uncultured archeon clones (1B6:CR626858.1, 4B7:CR626856.1, 22i07:JQ768096.1 and 19c08:JQ768095.1) were collected from NCBI BioSample database for the construction of digital data on tRNA. tRNAscan-SE 2.0 and ENDMEMO tools were used to identify and sketch tRNA structure as well as calculate Guanine-Cytosine (GC) percentage respectively. Eight true/functional tRNAs were identified from above 4 sequences which showed cove score greater than 20% with no variable loop. The tRNAs from the uncultured archeon clones were classified as Ala, Arg, Ile, Thr, Pro and Val type tRNA with cove score ranging from 34.22%-79.03%. The range of GC content was found 42.89%-56.91%; while tRNA contributed GC content ranging from 52%-64.86% to the total GC content in these sequences. The data fabricated in this study could be very useful for studying the diversity of tRNA among prokaryotes.

Genomics dataset on unclassified published organism (patent US 7547531).

Nucleotide (DNA) sequence analysis provides important clues regarding the characteristics and taxonomic position of an organism. With the intention that, DNA sequence analysis is very crucial to learn about hierarchical classification of that particular organism. This dataset (patent US 7547531) is chosen to simplify all the complex raw data buried in undisclosed DNA sequences which help to open doors for new collaborations. In this data, a total of 48 unidentified DNA sequences from patent US 7547531 were selected and their complete sequences were retrieved from NCBI BioSample database. Quick response (QR) code of those DNA sequences was constructed by DNA BarID tool. QR code is useful for the identification and comparison of isolates with other organisms. AT/GC content of the DNA sequences was determined using ENDMEMO GC Content Calculator, which indicates their stability at different temperature. The highest GC content was observed in GP445188 (62.5%) which was followed by GP445198 (61.8%) and GP445189 (59.44%), while lowest was in GP445178 (24.39%). In addition, New England BioLabs (NEB) database was used to identify cleavage code indicating the 5, 3 and blunt end and enzyme code indicating the methylation site of the DNA sequences was also shown. These data will be helpful for the construction of the organisms' hierarchical classification, determination of their phylogenetic and taxonomic position and revelation of their molecular characteristics.