RESUMEN
CONTEXT: The aerial parts of Sphagneticola trilobata (L.) Pruski (Asteraceae) are popularly used to treat topical inflammation, but have not been fully investigated. OBJECTIVE: To identify polar compounds in S. trilobata extracts and develop a new topical phytomedicine based on the kaurenoic acid (KA) content while monitoring and demonstrating its topical anti-inflammatory activity. MATERIALS AND METHODS: Ethanol spray-dried extract of S. trilobata was analysed by LC-MS while the KA content from semisolid was analysed by LC-UV. The extent of ear edema induced by applying 20 µL of croton oil (2.5%), arachidonic acid (AA; 2 mg/ear) and decanoylphorbol-13-acetate (TPA; 2.5 mg/ear) in mice was used to evaluate the biological activity of the semisolids, which were applied 30 min before the phlogistic agents. RESULTS: Eight phenylpropanoids and four oleanane-type triterpenoid saponins were identified, majority of them reported for the first time in this species, in addition to KA. The semisolid containing 1.0% of dried extract reduced the ear edema induced by croton oil [77.2 ± 4.5%; ID50 = 0.49 (0.28-0.87%)], TPA (81.5 ± 2.4%) and AA (39.1 ± 6.9%), with decreasing effect at higher KA concentrations. This was accompanied by neutrophil migration inhibition as investigated by biochemical and histological assays. DISCUSSION AND CONCLUSION: The anti-inflammatory effects were (at least in part) due to the interference in protein kinase C (PKC) activation, AA-cascade products and neutrophil migration inhibition, demonstrating the efficacy of the folk topical usage of this plant. The results support the development of a novel topical anti-inflammatory phytomedicine properly standardized to treat inflammatory dermatological diseases.
Asunto(s)
Antiinflamatorios/farmacología , Asteraceae , Fitoterapia , Extractos Vegetales/farmacología , Administración Tópica , Animales , Asteraceae/química , Movimiento Celular/efectos de los fármacos , Diterpenos/análisis , Diterpenos/farmacología , Masculino , Ratones , Neutrófilos/efectos de los fármacos , Neutrófilos/fisiología , Ácido Oleanólico/análisis , Extractos Vegetales/análisis , Proteína Quinasa C/metabolismoRESUMEN
BACKGROUND: Myrsinoic acid B (MAB) is a diprenylated benzoic acid widely found in the vegetal kingdom. Recent studies demonstrate that MAB has important antinociceptive effects in models of chemically or thermally induced nociception in mice. METHODS: In the present study we evaluated the effect of MAB in different models of inflammatory and neuropathic hypersensitivity in mice. RESULTS: This study demonstrates that the pretreatment with MAB, given orally (8.4 to 83.8 µmol/kg), inhibited carrageenan- and complete Freund adjuvant-induced mechanical hypersensitivity. When administered after the induction of hypersensitivity, MAB also reduced the mechanical hypersensitivity in the ipsilateral and in the contralateral hindpaws of mice injected with complete Freund adjuvant, interfering with a signaling cascade already established. MAB reversed the hypersensitivity (mechanical and thermal) of operated animals, with similar results to those observed with gabapentin. MAB activity was evident when administered either systemically (PO or IV) or intrathecally, suggesting interference in the central pathways of pain control. Furthermore, MAB seems to present an antiinflammatory effect evidenced by the interference in both the neutrophil migration and in the increase of interleukin-1ß levels after carrageenan injection. Of note, MAB treatment did not interfere with mechanical or thermal sensitivity in healthy mice, a frequent characteristic of commonly used analgesics, such as morphine or gabapentin. Side effects including interference in locomotor activity, motor performance, and body temperature in animals treated with MAB were absent. CONCLUSIONS: MAB reduced mechanical and thermal hypersensitivity in mice submitted to models of inflammatory and neuropathic pain, showing excellent potential for treating persistent pain in humans.
Asunto(s)
Alquenos/farmacología , Analgésicos/farmacología , Conducta Animal/efectos de los fármacos , Benzofuranos/farmacología , Hiperalgesia/prevención & control , Inflamación/complicaciones , Neuralgia/prevención & control , Dolor/prevención & control , Administración Oral , Alquenos/administración & dosificación , Alquenos/toxicidad , Analgésicos/administración & dosificación , Analgésicos/toxicidad , Animales , Benzofuranos/administración & dosificación , Benzofuranos/toxicidad , Carragenina , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Femenino , Adyuvante de Freund , Hiperalgesia/diagnóstico , Hiperalgesia/etiología , Hiperalgesia/metabolismo , Hiperalgesia/fisiopatología , Hiperalgesia/psicología , Inflamación/inducido químicamente , Inyecciones Intravenosas , Interleucina-1beta/metabolismo , Ratones , Actividad Motora/efectos de los fármacos , Neuralgia/diagnóstico , Neuralgia/etiología , Neuralgia/metabolismo , Neuralgia/fisiopatología , Neuralgia/psicología , Infiltración Neutrófila/efectos de los fármacos , Dolor/diagnóstico , Dolor/etiología , Dolor/metabolismo , Dolor/fisiopatología , Dolor/psicología , Dimensión del Dolor , Umbral del Dolor/efectos de los fármacos , Peroxidasa/metabolismo , Factores de TiempoRESUMEN
Cipura paludosa (Iridaceae) is a plant that is distributed in the north region of Brazil. Its bulbs are used in folk medicine to treat inflammation and pain. Four naphthalene derivatives have been isolated from the bulbs of this plant. Three of them have been identified as the known naphthalene derivatives, eleutherine, iso-eleutherine, and hongkonin. The structure of the fourth and new component was determined as 11-hydroxyeleutherine by extensive NMR study. In addition, the IN VIVO effect of the two major compounds, eleutherine and iso-eleutherine, was evaluated in carrageenan-induced hypernociception and inflammation in mice. Eleutherine and iso-eleutherine (1.04-34.92 µmol/kg), dosed intraperitoneally (i.p.) or orally (p.o.), decreased the carrageenan-induced paw oedema (i.p. - inhibitions of 36 ± 7 % and 58 ± 14 %, respectively; p.o. - inhibitions of 36 ± 7 % and 58 ± 14 %, respectively). Iso-eleutherine, but not eleutherine, significantly reduced (inhibitions of 39 ± 4 %) the plasma extravasation induced by intradermal (i.d.) injection of carrageenan. Likewise, eleutherine and iso-eleutherine (1.04-34.92 µmol/kg, i.p. or p.o.) were also effective in preventing the carrageenan-induced hypernociceptive response (i.p. - inhibition of 59 ± 4 % and 63 ± 1 %, respectively; p.o. - inhibitions of 36 ± 7 % and 58 ± 14 %, respectively). It was also suggested that the anti-inflammatory and anti-hypernociceptive effects of eleutherine or iso-eleutherine partly depend on the interference with the synthesis or activity of mast cell products, kinins, cytokine, chemokines, prostanoids, or sympathetic amines. Our findings show that two major compounds of C. paludosa contain pharmacologically active constituents that possess antinociceptive and anti-inflammatory activity, justifying, at least in part, its popular therapeutic use for treating conditions associated with pain.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Iridaceae/química , Naftoquinonas/química , Naftoquinonas/farmacología , Administración Oral , Animales , Antiinflamatorios no Esteroideos/química , Brasil , Carragenina/toxicidad , Evaluación Preclínica de Medicamentos , Edema/tratamiento farmacológico , Femenino , Inflamación , Inyecciones Intraperitoneales , Espectroscopía de Resonancia Magnética , Masculino , Ratones , Estructura Molecular , Naftoquinonas/administración & dosificación , Naftoquinonas/aislamiento & purificación , Dolor/tratamiento farmacológico , Raíces de Plantas/química , Plantas Medicinales/químicaRESUMEN
ETHNOPHARMMACOLOGICAL RELEVANCE: Aleurites moluccana is used in folk medicine to treat pain, fever, asthma, hepatitis, gastric ulcer and inflammatory process in general, and the nut oil had been topically applied to treat arthritis and other joint pain, however the seeds are classified as toxic for oral use. AIM: Faced with the need for new alternative to treat the symptoms and modify rheumatoid arthritis (RA) the aim of this work was to evaluate the effects of A. moluccanus' leaves dried extract in rats and mice submitted to complete Freund adjuvant (CFA)-induced RA. MATERIAL AND METHODS: Wistar Rats and Swiss mice were submitted to CFA-induced RA in the right hindpaw. They received A. moluccanus extract (orally; p.o.), dexamethasone (subcutaneously), 2â³-O-rhamnosylswertisin (p.o.) or vehicle (p.o.), from the 14th day after the CFA injection for up to 8 days. The mechanical hypersensitivity was evaluated using the von Frey filaments and the paw-oedema was measured using a plethysmometer. The rats' injected hindpaw was used to perform the histological analysis. RESULTS: A. moluccanus was able to significantly reduce the mechanical hypersensitivity in both ipsi- and contralateral hindpaws of mice injected with CFA, in a dose dependent manner. Furthermore, the paw-oedema was progressively reduced by A. moluccanus. Similar results were obtained for the positive-control drug dexamethasone and the isolated compound 2â³-O-rhamnosylswertisin. Besides the effects mentioned above, the extract was also effective to repair the joint damage in CFA-induced RA rats, including reduction of fibrosis, cartilage degradation and bone erosion scores. CONCLUSION: These results together with the literature data reinforce the anti-hypersensitivity and anti-inflammatory activity of A. moluccanus extract. Part of the observed effects is due to the presence of the compound 2â³-O-rhamnosylswertisin. The fact that the extract acted as a disease modifier point this herbal product as a promisor and safe tool to treat RA and other associated chronic diseases.
Asunto(s)
Aleurites/química , Artritis Experimental/tratamiento farmacológico , Flavonas/farmacología , Extractos Vegetales/farmacología , Ramnosa/análogos & derivados , Animales , Antirreumáticos/aislamiento & purificación , Antirreumáticos/farmacología , Artritis Experimental/patología , Artritis Reumatoide/tratamiento farmacológico , Artritis Reumatoide/patología , Edema/tratamiento farmacológico , Edema/patología , Flavonas/aislamiento & purificación , Adyuvante de Freund/administración & dosificación , Masculino , Medicina Tradicional/métodos , Ratones , Extractos Vegetales/aislamiento & purificación , Hojas de la Planta , Ratas , Ratas Wistar , Ramnosa/aislamiento & purificación , Ramnosa/farmacologíaRESUMEN
Steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) are widely used to control inflammatory pain, but there is a risk of gastrointestinal bleeding and increased heart failure risk. The search for new drugs remains ongoing, and natural products are a source for potential new compounds. Mangiferin, a natural xanthone C-glucoside, has demonstrated biological activity, including anti-inflammatory and analgesic properties, but it's mechanisms are poorly understood. In this study, we investigated the mechanisms underlying the anti-inflammatory and analgesic effects of local administration of mangiferin. We employed an electronic von Frey apparatus to evaluate mechanical hyperalgesia induced by carrageenan in rats. Mangiferin (150-1200⯵g/paw), administered locally into the hindpaw, prevented hyperalgesia in a dose-dependent - 150⯵g (- 9%), 300⯵g (- 27%, Pâ¯<â¯0.01), 600⯵g (- 77%, Pâ¯<â¯0.001) and 1000⯵g (- 93%, Pâ¯<â¯0.001) - and local manner. Mangiferin showed decreased levels of TNF-α (Pâ¯<â¯0.001) and CINC-1 (Pâ¯<â¯0.001), but not IL-1ß; it also prevented neutrophil migration (Pâ¯<â¯0.01), but not the increased COX-2 expression in peripheral tissue challenged with carrageenan. To further explore the mechanisms of mangiferin actions, rats were injected with modulators of inflammation and nociception; mangiferin prevented hyperalgesia induced by IL-1ß (Pâ¯<â¯0.01), CINC-1 (Pâ¯<â¯0.01), epinephrine (Pâ¯<â¯0.01), 8-Br-cAMP (Pâ¯<â¯0.01) or capsaicin (Pâ¯<â¯0.01), but not that induced by PGE2 or α,ß-MeATP. Our study shows that mangiferin has anti-inflammatory and analgesic properties when locally administrated. The control of the inflammatory response and mechanical hyperalgesia by mangiferin depends on the inhibition of TNF-α production/release and the CINC1/epinephrine/PKA pathway, supporting its marked inhibition of inflammatory mechanical hyperalgesia.
Asunto(s)
Analgésicos , Antiinflamatorios , Hiperalgesia , Xantonas , Analgésicos/farmacología , Analgésicos/uso terapéutico , Animales , Antiinflamatorios/farmacología , Antiinflamatorios/uso terapéutico , Carragenina , Quimiocina CXCL1/metabolismo , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Dinoprostona/metabolismo , Epinefrina/metabolismo , Hiperalgesia/inducido químicamente , Hiperalgesia/tratamiento farmacológico , Hiperalgesia/inmunología , Hiperalgesia/metabolismo , Interleucina-1beta/metabolismo , Masculino , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Ratas Wistar , Factor de Necrosis Tumoral alfa/metabolismo , Xantonas/farmacología , Xantonas/uso terapéuticoRESUMEN
The treatment of chronic pain remains a challenge for clinicians worldwide, independent of its pathogenesis. It motivates several studies attempting to discover strategies to treat the disease. The in silico analysis using molecular docking approach demonstrated that the phthalimide N-(4methyl-phenyl)-4-methylphthalimide (MPMPH-1) presented high affinity to adenylyl-cyclase enzyme (AC). It also prominently reduced the mechanical hypersensitivity of mice challenged by Forskolin, an AC activator. This effect lasted for up to 48h after Forskolin injection, presenting activity longer than MDL-12330A (AC inhibitor). MPMPH-1 was also effective in reducing the hypersensitivity induced by IL-1ß, bradykinin, prostaglandin E2 or epinephrine, chemical mediators that have, among others, AC as pivotal protein in their signalling cascade to induce mechanical-pain behaviour. The compound presented marked inhibition in inflammatory-pain models induced by carrageenan, lipopolysaccharide or complete Freund's adjuvant, including neutrophil migration inhibition. Furthermore, it also seems to act in both peripheral and pain central-control pathways, being also effective in reducing the persistent cancer-pain behaviour induced by melanoma cells in mice. MPMPH-1 could represent a promising pharmacological tool to treat acute and chronic painful diseases, with good bioavailability, local activity, and lack of locomotor-activity interference. Further studies are necessary to determine the exact mechanism of action but it seems to involve AC enzyme as possible target.