Shear thinning three-dimensional colloidal assemblies of chitosan and poly(lactic acid) nanoparticles.

In this study, new materials capable of reversible self-assembly, based on concentrated negatively charged poly(lactic acid) nanoparticles and chitosan, a natural polycationic polymer, were successfully fabricated. Electrostatic interactions between oppositely charged components along with weaker interactions led to the formation of a 3D network. The resulting macroscopic assemblies were characterized by dynamic mechanical measurements, and the influences of various parameters such as chitosan/poly(lactic acid) weight ratio, duration and temperature of the mixture, and molecular weight or chitosan degree of acetylation were studied. Our results showed that the mechanical properties of assemblies were highly dependent on the nanoparticle solid content and chitosan/nanoparticle ratio. In particular, at an optimum weight ratio the colloidal assemblies exhibited remarkable high elastic moduli (about 300 kPa) for a particle solid content of 18% w/w. Thanks to the weak and reversible nature of the interactions, these materials exhibited shear thinning properties, and could instantly recover their cohesiveness at rest. The mode of interactions between PLA particles and chitosan was shown to be in part due to electrostatic interactions, but the cross-linking of chitosan-covered particles was attributed to hydrogen bonding. These materials could be envisaged as good candidates for injectable scaffolds for tissue engineering, taking advantage of the biocompatibility and bioactivity of both components. However, some issues concerning temperature stability must be resolved before applying these colloidal assemblies to cell growth in physiological conditions.

Facile and Rapid Access to Glyconanocapsules by CuAAC Interfacial Polyaddition in Miniemulsion Conditions.

Glyconanocapsules with a biocompatible oily core have been successfully prepared by copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) interfacial step growth polymerization between 6,6'-diazido-6,6'-dideoxysucrose and bis(propargyloxy)butane in oil-in-water miniemulsion conditions. Optimization of the interfacial polymerization process in dispersed medium afforded the rapid and reproducible preparation of stable monodispersed glyconanocapsules having a diameter around 200 nm.