RESUMEN
BACKGROUND: Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible. RESULTS: In this regard, two DnaJs (Cn-DnaJa1 and Cn-DnaJb1) are cloned from the Coilia nasus's ovary. Their structure both contains J domain, G/F domain and ZF domain. Their mRNA transcripts were found extensively expressed in all the sampled tissues and significantly highly in gonads, which probably mean that DnaJs involved in C. nasus's gonad development basal metabolic processes. In the process of spawning migration, Cn-DnaJa1 and Cn-DnaJb1 mRNA transcripts were also expressed with significant differences during oogenesis with highest levels in the development phase, and maintaining high levels during the multiplication, mature and spawning phase. Further study showed that the DnaJa1and DnaJb1protein have high distribution in the onset phase and mainly distributed in the oocyte cytoplasm especially during the migration development phase's. CONCLUSIONS: This experiment study demonstrated that DnaJs participate in reproductive regulation during the spawning migration process in C. nasus and possibly play a vital role in the ovary development process. These findings also provided a base knowledge for further molecular mechanism study of spawning migration.
Asunto(s)
Migración Animal/fisiología , Peces/embriología , Peces/genética , Proteínas del Choque Térmico HSP40/genética , Oogénesis/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Femenino , Oogénesis/fisiología , Ovario , Dominios Proteicos/genética , ARN Mensajero/genéticaRESUMEN
We report a continuous-wave dual-wavelength Nd:YAG laser at 1052 and 1073 nm, for the first time to the best of our knowledge, in free-running mode. The maximum output power reaches 6.64 W with slope efficiency of about 42.1% with respect to the absorbed power. Inserting a Cr:YAG saturable absorber, the dual-wavelength laser can operate in the Q-switching regime with maximum average output power of 880 mW. The minimum pulse width and the highest pulse repetition rate are 28 ns and 17.12 kHz, respectively. The dual-wavelength laser has potential to generate a 5.7 THz wave via the difference-frequency method.
RESUMEN
Blood sugar is an important biomedical parameter of diabetic patients. The current blood sugar testing is based on an invasive method, which is not very friendly for patients who require long-term monitoring, while the non-invasive method is still in the developing stage. In this paper, we design a non-invasive and highly sensitive terahertz wave detector with Co3Sn2S2 semimetal thin film to test sugar concentration. As different concentrations have inconsistent responses to terahertz wave, we can deduce the concentration of the sugar solution to realize real-time highly sensitive detection of blood sugar concentration. This novel method can be further expanded to 6â G edge intelligence for non-invasive and real-time monitoring of blood sugar, and promote the development of 6â G technology.