[1,25-Dihydroxyvitamin D3 receptors in lymphocytes and T- and B-lymphocyte count in patients with glomerulonephritis]. / Retseptory 1,25-digidroksivitamina D3 v limfotsitakh i uroven' T- i B-limfotsitov u bol'nykh glomerulonefritom.

Content of receptors to hormonal form of vitamin D3, 1.25(OH)2D3, constituted 27.3 fmole/mg of protein in lymphocytes of peripheric blood of children with glomerulonephritis. In the patients concentration of total and ionized form of Ca2+ was decreased down to 2.04 mmole/L and 1.09 mmole/L, respectively, while an increase in parathormone (PTH) by 36% and a distinct decrease in 25(OH)D concentration (lower than 1.25 ng/ml) was found in blood; content of cAMP was also decreased in lymphocytes by 33%. At the same time, total content of T lymphocytes was decreased 1.5-fold in peripheric blood. Treatment with I-hydroxyvitamin D3 (1-1.5 mg daily, within 4 weeks) led to normalization of total and ionized form of Ca2+ and of 25(OH)D, but did not affect the PTH content in blood. Concentration of the receptors to 1.25(OH)2D3 was elevated up to 39.7 fmole/mg after I week of the treatment, whereas it was decreased to the initial level 24.8 fmole/mg within 4 weeks; simultaneous alteration in the cAMP content was observed in lymphocytes. Treatment with 1-(OH)D3 normalized also the T lymphocytes content in peripheric blood. The data obtained suggest that under conditions of glomerulonephritis only high content of receptors to 1.25(OH)2D3 in lymphocytes enabled to perform the cell response to the hormone effect.

[The effect of 24,25-dihydroxyvitamin D3 (dioxyvit) on Ca metabolism and immune status during chronic kidney failure]. / Vliianie 24,25-digidroksivitamina D3 (dioksivita) na obmen Ca i immunnyi status pri khronicheskoi pochechnoi nedostatochnosti.

Active metabolite of vitamin D3, 24R,25 (OH)2D3 (dioxyvit) was used at a daily dose of 100 micrograms in treatment of children affected with tubulointerstitial disease of kidney and with chronic glomerulonephritis under conditions of kidney insufficiency. The drug exhibited distinct normalizing effect on patterns of calcium metabolism: increase of total and ionized Ca2+ and of 25-OHD, decrease in concentration of parath hormone and osteocalcine in blood serum as well as on immunological parameters: restoration of decreased content of T- and 0-lymphocytes. Concentration of receptors of hormonal form of 1,25(OH)2D3 was found to be minimal in lymphocytes under conditions of chronic kidney insufficiency, while their expression, after the dioxyvit action, was detected only in patients with glomerulonephritis. Specific calcitropic effect of dioxyvit with simultaneous correction of vitamin D deficiency were apparently responsible for high efficacy of the drug in treatment of calcium metabolism and immunity impairments in children with renal deteriorations at the step of chronic kidney insufficiency.

[Development of vitamin D deficiency and immunologic disorders in children with glomerulonephritis]. / Razvitie D-vitaminnoi nedostatochnosti i immunologicheskikh narushenii pri glomerulonefrite u detei.

Biochemical symptoms of vitamin D deficiency and a sharp reduction in the number of T-lymphocytes in the peripheral blood were recorded in children suffering from glomerulonephritis. During the combined therapy using the compound "oksidevit " (1-hydroxyvitamin D3) the parameters characterizing D-vitamin providing became normal, and the number of T-lymphocytes in the peripheral blood was recovered.

[Effect of the treatment with oxydevit on calcium metabolism, immune status and 1,25(OH)2D3 receptors in the lymphocytes of children with glomerulonephritis]. / Vliianie lecheniia oksidevitom na obmen kal'tsiia, immunnyi status i retseptory 1,25(OH)2D3 v limfotsitakh u detei s glomerulonefritom.

In all the examined children with chronic glomerulonephritis (GN), peripheral blood lymphocytes had a high concentration of receptors of 1,25 (OH)2D3, a hormonal form of vitamin D. At the same time as to the healthy children, 1,25 (OH)2D3 receptors were identified only in 27%, with the concentration being lower. A large amount of 1,25 (OH)2D3 receptors may be related to the expression of the hormone in activated lymphocytes. In addition to the normalization of calcium metabolism, the treatment with oxydevit favoured the normalization of the content of IgG and T lymphocytes as well as a reduction (to normal) of the content of 0 lymphocytes regardless of the previous treatment. Partial remission was attained in 14 out of 20 GN patients. In 10 of these children, the remission lasting 1-2 years was attained for the first time. In 17 children treated with oxydevit, the incidence of acute respiratory viral infection decreased from 6-8 times to 1-2 times a year. The data obtained allowed one to ascertain the immunomodulatory capacities of oxydevit in children with chronic glomerulonephritis.

[Use of vitamin D 2 and its metabolites in chronic glomerulonephritis in children]. / Primenenie vitamina D2 i ego metabolitov pri khronicheskom glomerulonefrite u detei.

Comparative assessment of the action of vitamin D2 in high doses, of oxydevit and rocaltrol applied in renal osteopathies has demonstrated that they are equally effective in elevating the calcium concentration in blood serum occurring at the expense of its increased absorption by the intestine. At the same time certain differences have been discovered in the action of these agents on endocrine regulation. The data obtained provide evidence in favour of the use of vitamin D2 in high doses or of its metabolites not only in renal osteopathies at the stage of chronic renal failure but also in the management of the disorders of phosphorus-calcium metabolism in patients with the nephrotic syndrome in the functional-compensation stage after continuous use of corticosteroids, heparin and diuretics.