RESUMEN
Saccharothrix algeriensis NRRL B-24137 is an actinobacterium isolated from Algerian Saharan soil. It produces bioactive compounds belonging to the dithiolopyrrolone class of antibiotics, which are characterized by the possession of a unique pyrrolinonodithiole nucleus. Dithiolopyrrolones are known for their strong antibacterial and antifungal activities. This class of antibiotics generated great interest after the discovery of their anticancer properties. In this study, an antibiotic named PR11, produced after a long bacterial fermentation (11 days) in sorbic acid-containing culture broth, was characterized as a new dithiolopyrrolone derivative. After HPLC analysis and purification, the chemical structure of this antibiotic was determined by 1 H- and 13 C-nuclear magnetic resonance, mass and UV-visible data. PR11 was thus characterized as an iso-hexanoyl-pyrrothine, a novel dithiolopyrrolone derivative. The minimum inhibitory concentrations of the new induced antibiotic were determined against several pathogenic micro-organisms. A moderate to strong activity was noted against all Gram-positive bacteria, filamentous fungi and yeasts tested. SIGNIFICANCE AND IMPACT OF THE STUDY: Given the strong activities of dithiolopyrrolones against diverse prokaryotic and eukaryotic micro-organisms including potent selective-anticancer activity, the discovery of new-related derivatives draw continuous attention for therapeutic research. Depending on nature and concentration of added precursor, Saccharothrix algeriensis NRRL B-24137 produce several dithiolopyrrolone coumpounds. In this study, sorbic acid addition combined to long fermentation duration was shown to induce the biosynthesis of a novel dithiolopyrrolone derivative. After purification and full spectroscopic and spectrometric study, the compound was characterized as iso-hexanoyl-pyrrothine. In the future investigation for novel dithiolopyrrolone discovery, fermentation duration should be regarded as a key parameter as well.
Asunto(s)
Actinobacteria/metabolismo , Antiinfecciosos/farmacología , Hongos/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Pirroles/farmacología , Fermentación , Pruebas de Sensibilidad Microbiana , Ácido Sórbico/metabolismoRESUMEN
Dithiolopyrrolone antibiotics, produced by several micro-organisms, are known for their strong antimicrobial and antitumor activities. Among of this micro-organisms, Saccharothrix algeriensis NRRL B-24137, a rare actinobacterium, has the ability to produce several dithiolopyrrolones derivatives depending on precursors added in the culture medium. After 10 days of strain fermentation on semi-synthetic medium supplemented with cinnamic acid and HPLC purification, biosynthesis of benzoyl-pyrrothine dithiolopyrrolone was evidenced through complete spectroscopic (UV-visible and 1H and 13C NMR) and spectrometric (electron impact mass spectrum) analyses. The pure molecule showed appreciable minimum inhibitory concentration values against several Gram-positive bacteria and filamentous fungi. SIGNIFICANCE AND IMPACT OF THE STUDY: Dithiolopyrrolone antibiotics, known for their strong antimicrobial activities, gained greater interest after the discovery of their antitumor properties. Depending on precursors added, Saccharothrix algeriensis NRRL B-24137 has the ability to produce several dithiolopyrrolones derivatives. Since biological activities of dithiolopyrrolones are related to their variable structure, discover of new natural analogues to be therapeutically explored remains a significant framework of research. In this study, a new dithiolopyrrolone derivative was purified from the fermentation broth of S. algeriensis NRRL B-24137. This new antibiotic, characterized as benzoyl-pyrrothine dithiolopyrrolone, was induced by adding cinnamic acid, as precursor, to a semi-synthetic medium.
Asunto(s)
Actinomycetales/metabolismo , Antibacterianos/farmacología , Hongos/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Pirrolidinonas/farmacología , Actinomycetales/clasificación , Cinamatos/metabolismo , Medios de Cultivo/farmacología , Fermentación , Pruebas de Sensibilidad Microbiana , Pirrolidinonas/metabolismoRESUMEN
During screening for potentially antimicrobial actinobacteria, a highly antagonistic strain, designated WAB9, was isolated from a Saharan soil of Algeria. A polyphasic approach characterized the strain taxonomically as a member of the genus Streptomyces. The strain WAB9 exhibited a broad spectrum of antimicrobial activity toward various multidrug-resistant micro-organisms. A PCR-based assay of genomic potential for producing bioactive metabolites revealed the presence of PKS-II gene. After 6 days of strain fermentation, one bioactive compound was extracted from the remaining aqueous phase and then purified by HPLC. The chemical structure of the compound was determined by spectroscopic (UV-visible, and (1)H and (13)C NMR) and spectrometric analysis. The compound was identified to be 2-amino-N-(2-amino-3-phenylpropanoyl)-N-hydroxy-3-phenylpropanamide, a novel hydroxamic acid-containing molecule. The pure molecule showed appreciable minimum inhibitory concentration values against a selection of drug-resistant bacteria, filamentous fungi and yeasts. Significance and impact of the study: This study presents the isolation of a Streptomyces strain, named WAB9, from a Saharan soil in Algeria. This strain was found to produce a new hydroxamic acid-containing molecule with interesting antimicrobial activities towards various multidrug-resistant micro-organisms. Although hydroxamic acid-containing molecules are known to exhibit low toxicities in general, only real evaluations of the toxicity levels could decide on the applications for which this new molecule is potentially most appropriate. Thus, this article provides a new framework of research.
Asunto(s)
Antibacterianos/farmacología , Bacterias/efectos de los fármacos , Ácidos Hidroxámicos/farmacología , Streptomyces/metabolismo , Levaduras/efectos de los fármacos , Argelia , Antibacterianos/biosíntesis , Antibacterianos/química , Cromatografía Líquida de Alta Presión , Farmacorresistencia Bacteriana Múltiple/efectos de los fármacos , Farmacorresistencia Fúngica Múltiple/efectos de los fármacos , Ácidos Hidroxámicos/química , Ácidos Hidroxámicos/metabolismo , Pruebas de Sensibilidad Microbiana , Suelo , Microbiología del Suelo , Streptomyces/genética , Streptomyces/aislamiento & purificaciónRESUMEN
UNLABELLED: This work aimed to study the interaction between Actinomycetal isolates and Aspergillus flavus to promote mutual antagonism in contact. Thirty-seven soilborn Streptomyces spp. isolates were chosen as potential candidates. After a 10-day in vitro co-incubation period, 27 isolates respond to the criteria, that is, mutual antagonism in contact. Further aflatoxins B1 and B2 analysis revealed that those 27 isolates reduced aflatoxin B1 residual concentration from 38·6 to 4·4%, depending on the isolate. We selected 12 isolates and tested their capacity to reduce AFB1 in pure culture to start identifying the mechanisms involved in its reduction. AFB1 was reduced by eight isolates. The remaining AFB1 concentration varied between 82·2 and 15·6%. These findings led us to suggest that these eight isolates could be used as biocontrol agents against AFB1 and B2 with low risk of impacting the natural microbial equilibrium. SIGNIFICANCE AND IMPACT OF THE STUDY: Interaction between Aspergillus flavus and Actinomycetes isolates was conducted in vitro. Actinomycetes isolates having a mutual antagonism in contact with A. flavus were chosen for further aflatoxins production study. This is a new approach based to develop biocontrol against aflatoxins accumulation in maize while respecting natural microbial equilibrium.
Asunto(s)
Actinobacteria/fisiología , Aflatoxina B1/biosíntesis , Aflatoxinas/biosíntesis , Aspergillus flavus/metabolismo , Actinobacteria/aislamiento & purificación , Antibiosis , Aspergillus flavus/crecimiento & desarrollo , Microbiología de Alimentos , Zea mays/microbiologíaRESUMEN
UNLABELLED: During a screening for potential plant disease control actinomycetes, a total of 133 strains were isolated from Saharan soil samples of seven Algerian regions by dilution technique on chitin-vitamins agar medium. Screening for antagonistic properties using streak assay method showed that 25% of isolates demonstrated strong activities against a wide range of plant pathogenic fungi. Due to their strong anti-Fusarium activities, six of these isolates were selected and subsequently related to Streptomyces species by polyphasic analysis. These isolates were evaluated for their biocontrol ability against Fusarium culmorum, a serious pathogenic fungus of cereals crops related to damping-off and seedling blight resulting in yield loss. Barley seeds were chosen as cereal plant model. Surface bacterized seeds with TW3, RI3 and TW2 strains expressed the highest performances and permit to reduce significantly both the disease occurrence on seedlings (62-76%) and the extent of seedling blight symptoms (over than 95%). However, a negative effect on plant establishment was observed for RI3 treatment. SIGNIFICANCE AND IMPACT OF THE STUDY: The genus Fusarium is considered to be one of the most problematic phytopathogenic fungi for crop culture worldwide. Inside this genus, F. culmorum is the aetiological agent of seedling blight in various monocotyledonous plants such as barley and cause extensive yield and quality losses in humid and semi-humid regions. Biological control may be a successful alternative to chemical control, particularly with the controversy surrounding the use of the fungicides and the limited obtained results to control F. culmorum. This study highlights the effectiveness of some antagonistic Streptomyces isolated from Algerian Saharan soils to control F. culmorum by the reduction in disease occurrence and disease severity suggesting their use on microbial biocontrol formulation against soilborne diseases.
Asunto(s)
Antibiosis , Fusarium/patogenicidad , Hordeum/microbiología , Enfermedades de las Plantas/prevención & control , Streptomyces/fisiología , Antifúngicos/metabolismo , Productos Agrícolas/microbiología , Enfermedades de las Plantas/microbiología , Plantones/microbiología , Semillas/microbiología , Microbiología del Suelo , Streptomyces/clasificación , Streptomyces/aislamiento & purificaciónRESUMEN
Wheat is a basic staple food for very large segments of the population of Algeria. The aim of this study is to analyse ochratoxin A (OTA)-producing mould and OTA-contaminated wheat. To evaluate the mycoflora and the potential for OTA production by Aspergillus strains, a total of 85 samples of wheat destined for human consumption were collected from two regions in Algeria (Tizi Ouzou and Setif) during the following phases: preharvest, storage in silos, and after processing. The mean value counts of fungi ranged from 275 to 1277 CFU g(-1). The dominant genus was Aspergillus, predominantly A. flavus, A. niger and A. versicolor. The other isolated species were A. ochraceus, A. alliaceus, A. carbonarius, A. terreus, A. fumigatus, A. candidus and Aspergillus spp. The occurrence and the levels of the genus Penicillium, Fusarium, Alternaria and Mucor were substantially lower than those of Aspergillus. The storage in silos shows high levels of Aspergillus (66 to 84%), especially A. flavus, but A. niger and other fungi were isolated at relatively low percentages. Equal distribution of the fungal contamination into the bran, flour and semolina fractions was observed from Flour Mill and Semolina Mill. The genus Aspergillus remained present at high levels at several phases of the production process. In addition, the ability to produce OTA by 135 isolates belonging to eleven species of Aspergillus and 23 isolates of Penicillium spp. was analyzed using fluorescent detection-based HPLC. Thus, it was found that 51 isolates (32.3%) were ochratoxigenic. All isolated strains of A. ochraceus (12) and A. alliaceus (6) produced OTA at concentrations ranging from 0.23 to 11.50 microg g(-1). Most of the A. carbonarius strains (80%) were OTA producers (0.01 to 9.35 microg g(-1)), whereas A. terreus (50%), A. niger (28%), A. fumigatus (40%), A. versicolor (18%) and Penicillium spp. (21.7%) were low level producers (0.01 to 0.07 microg g(-1)). The concentration of OTA was determined in 30 samples of wheat. OTA was detected in 12 (40%) of the samples at levels ranging from 0.21 to 41.55 microg kg(-1).
Asunto(s)
Aspergillus/aislamiento & purificación , Contaminación de Alimentos/análisis , Manipulación de Alimentos/métodos , Conservación de Alimentos/métodos , Ocratoxinas/análisis , Triticum , Argelia , Aspergillus/crecimiento & desarrollo , Aspergillus/metabolismo , Cromatografía Líquida de Alta Presión/métodos , Recuento de Colonia Microbiana , Seguridad de Productos para el Consumidor , Humanos , Ocratoxinas/biosíntesis , Penicillium/crecimiento & desarrollo , Penicillium/aislamiento & purificación , Penicillium/metabolismo , Triticum/química , Triticum/microbiologíaRESUMEN
An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.3% with Streptomyces gancidicus NBRC 15412T. The comparison of its cultural and physiological characteristics with this species revealed significant differences. Moreover, the phylogenetic tree showed that strain HG29 forms a distinct phyletic line within the genus Streptomyces. Production of antifungal activity was investigated by following kinetics in shake broth. The highest antifungal activity was obtained after five days of fermentation, and in the dichloromethane extract. Two active compounds, NK1 and NK2, were purified by HPLC using a C18 column. Their chemical structures were identified through nuclear magnetic resonance experiments and mass spectrometry as oligomycins E and A, respectively, which have not been reported to be produced by S. gancidicus. The two bioactive compounds exhibited significant antifungal activity in vitro, showing minimal inhibitory concentrations (MICs) values between 2 and 75µg/mL.
Asunto(s)
Oligomicinas/química , Microbiología del Suelo , Suelo/química , Streptomyces/química , Streptomyces/aislamiento & purificación , África del Norte , Argelia , Antifúngicos/farmacología , Fermentación , Hongos/clasificación , Hongos/efectos de los fármacos , Hongos/aislamiento & purificación , Hongos/patogenicidad , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Oligomicinas/aislamiento & purificación , Oligomicinas/farmacología , Filogenia , ARN Ribosómico 16S/genética , Metabolismo Secundario , Análisis de Secuencia de ADN , Streptomyces/clasificación , Streptomyces/genéticaRESUMEN
A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500(T) (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.
Asunto(s)
Actinomycetales/aislamiento & purificación , Actinomycetales/metabolismo , Antifúngicos/metabolismo , Antifúngicos/farmacología , Hongos/efectos de los fármacos , Microbiología del Suelo , Actinomycetales/genética , África del Norte , Argelia , Técnicas de Tipificación Bacteriana , ADN Ribosómico/análisis , Hongos/crecimiento & desarrollo , Hongos/patogenicidad , Pruebas de Sensibilidad MicrobianaRESUMEN
In a search for new antifungal antibiotics active against Candida albicans and others pathogenic fungi, a strain of actinobacteria, designated G61, was isolated from a Saharan soil and tested for its activity against these microorganisms. The analysis of its 16S rDNA sequence showed a similarity level of 100% with Streptomyces mutabilis NBRC 12800(T). The highest anticandidal activities produced by the strain G61 were obtained on Bennett medium in the fourth day of incubation. The active product, extracted by n-butanol, contained one bioactive spot detected on thin layer chromatography plates. It was purified by HPLC and its chemical structure was determined by spectroscopic analyses as 2,4-Di-tert-butylphenol. The minimum inhibitory concentrations (MIC) of this product against several strains of pathogenic microorganisms are interesting.
Asunto(s)
Antifúngicos , Candida albicans/efectos de los fármacos , Hongos/efectos de los fármacos , Fenoles/metabolismo , Fenoles/farmacología , Streptomyces/metabolismo , África del Norte , Antifúngicos/aislamiento & purificación , Antifúngicos/metabolismo , Antifúngicos/farmacología , Candida albicans/crecimiento & desarrollo , Candida albicans/patogenicidad , Hongos/crecimiento & desarrollo , Hongos/patogenicidad , Pruebas de Sensibilidad Microbiana , Fenoles/aislamiento & purificación , ARN Ribosómico 16S/análisis , Análisis de Secuencia de ADN , Microbiología del Suelo , Streptomyces/clasificación , Streptomyces/genética , Streptomyces/aislamiento & purificaciónRESUMEN
A new strain of actinomycete designated PAL114, producing antimicrobial compounds, was isolated from a Saharan soil in Ghardaïa, Algeria. Morphological and chemical studies showed that this strain belonged to the genus Streptomyces. Two bioactive compounds, named P41A and P41B, were extracted by dichloromethane from the cell-free supernatant broth of strain PAL114 and were purified by HPLC. Minimum inhibitory concentrations of the pure antibiotics were determined against yeasts, filamentous fungi and bacteria, most of which are pathogenic or toxigenic for human and multiresistant to antibiotics. The strongest activities were observed against Candida albicans M3 and Bacillus subtilis ATCC 6633. The chemical structures of the compounds were determined by spectroscopic analysis of UV-visible and 1H and 13C NMR spectra and spectrometric analysis of mass spectrum. The compounds P41A and P41B were identified as saquayamycins A and C, respectively. These compounds belong to the aquayamycin-group antibiotics, which are known in the literature for their anticancer and antibacterial activities.
Asunto(s)
Antiinfecciosos/farmacología , Streptomyces/química , África del Norte , Argelia , Antraquinonas/aislamiento & purificación , Antraquinonas/farmacología , Bacillus subtilis/efectos de los fármacos , Bacillus subtilis/crecimiento & desarrollo , Candida albicans/efectos de los fármacos , Candida albicans/crecimiento & desarrollo , Humanos , Pruebas de Sensibilidad Microbiana , Suelo , Microbiología del Suelo , Streptomyces/aislamiento & purificación , Streptomyces/metabolismoRESUMEN
Actinomycete strain SA198, isolated from a Saharan soil sample of Algeria, exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria, and phytopathogenic and toxinogenic fungi. The morphological and chemotaxonomic characteristics of the strain were consistent with those of the genus Saccharothrix. Analysis of the 16S rRNA gene sequence of strain SA198 showed a similarity level ranging between 97.2 and 98.8% within Saccharothrix species, S. australiensis being the most closely related. Two new active products were isolated by reverse HPLC using a C18 column. The ultraviolet-visible (UV-VIS), infrared (IR), mass, and (1)H and (14)C nuclear magnetic resonance (NMR) spectra showed that these products were new bioactive compounds. The minimum inhibitory concentrations of these antibiotics showed a strong activity against fungi and moderate activities against Gram-positive and Gram-negative bacteria.
Asunto(s)
Actinomycetales/clasificación , Actinomycetales/aislamiento & purificación , Antibacterianos/metabolismo , Microbiología del Suelo , Actinomycetales/genética , Actinomycetales/metabolismo , África del Norte , Bacterias/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , FilogeniaRESUMEN
OBJECTIVE: Study of the taxonomy and the biological activity of the actinomycete strain PAL111 against several pathogenic and toxigenic microorganisms for humans, and resistant to many antibiotics. MATERIALS AND METHODS: The taxonomic study of isolate PAL111 is carried out on the basis of phenotypic and molecular characteristics. The tests against the pathogenic microorganisms are realized on ISP-2 and Bennett media. The kinetics of antibiotic production was investigated on ISP-2 medium. The antibiotic is highlighted by bioautography and chemical revelations, and then purified by chromatography on thick layer of silica gel and Sephadex LH20 column. The minimum inhibitory concentrations (MIC) were determined against pathogenic microorganisms. RESULTS: The phenotypic and molecular studies showed that the isolate PAL111 is closely related to the type strain of Streptomyces ambofaciens. It showed a strong activity against Candida albicans, filamentous fungi, and Gram-positive and Gram-negative bacteria. The optimal antibiotic production was observed at the end of the exponential phase of growth and at the beginning of the decline phase. The bioautography tests showed the presence of an antibiotic with both antibacterial and antifungal activities. This antibiotic is a hydrophilic amino-glycoside compound. The MIC were observed between 2 and 20µg/mL for yeasts, 10 and 50µg/mL for filamentous fungi, 2 and 10µg/mL for Gram-positive bacteria, and 20 and 75µg/mL for Gram-negative bacteria. CONCLUSION: The strong activity of isolate PAL111 against the pathogenic microorganisms and the polar characteristic of the produced antibiotic could encourage further studies on this bioactive molecule.
Asunto(s)
Antibiosis/fisiología , Farmacorresistencia Microbiana , Streptomyces/fisiología , Levaduras/patogenicidad , Humanos , Pruebas de Sensibilidad Microbiana , Filogenia , Streptomyces/química , Streptomyces/clasificación , Levaduras/efectos de los fármacosRESUMEN
AIMS: Identification of a new actinomycete strain Sg3, belonging to the genus Streptosporangium and partial characterization of the produced antibacterial activities. METHODS AND RESULTS: The strain Sg3 was isolated from an Algerian Saharan soil and identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Streptosporangium. The comparison of its physiological characteristics with those of known species of Streptosporangium showed significant differences with the nearest species Streptosporangium carneum. Analysis of the 16S rDNA sequence of strain Sg3 showed a similarity level ranging between 97% and 98.8% within Streptosporangium species, with S. carneum the most closely related. Strain Sg3 showed a red coloured antibacterial activity against gram-positive bacteria on several culture media. The purification of the red pigment by chromatographic methods led to the isolation of three active products. The (1)H nuclear magnetic resonance (NMR), mass, infrared (IR) and ultraviolet-visible (UV-VIS) data of these molecules strongly suggested that they belonged to the quinone-anthracycline group with three or more rings. CONCLUSIONS: Strain Sg3 represents a distinct phyletic line suggesting a new genomic species. It produces antibacterial activities identified as quinone-anthracycline aromatics. SIGNIFICANCE AND IMPACT OF THE STUDY: The quinone-anthracycline antibiotics are known for their antimicrobial and antineoplastic activities and are used in chemotherapy for the treatment of many cancer diseases. The present work constitutes the first stage of a whole series of studies to be realized on these antibiotics before arriving at a possible application.
Asunto(s)
Actinobacteria/metabolismo , Antibacterianos/biosíntesis , Microbiología del Suelo , Actinobacteria/clasificación , Actinobacteria/aislamiento & purificación , Actinobacteria/fisiología , Actinobacteria/ultraestructura , Argelia , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antibacterianos/farmacología , Medios de Cultivo/farmacología , Pruebas de Sensibilidad Microbiana , FilogeniaRESUMEN
During a search for nonpolyenic antifungal antibiotics, an actinomycete designated AC104 was isolated from a Saharan soil sample by a dilution agar plating method using a chitin - vitamins B medium supplemented with rifampicin. Isolate AC104 presented the morphological and the chemical characteristics of the genus Actinomadura. On the basis of 76 physiological tests and 16S rDNA analysis, this isolate was determined to be quite different from the known species of Actinomadura. It is active against filamentous fungi and both Gram-positive and Gram-negative bacteria. The production of antibiotic substances was investigated using several culture media. The highest antimicrobial activities were obtained on ISP2 medium. The benzenic extract contained five bioactive spots detected on thin layer chromatography plates. Among these antibiotics, a complex called 104A, which showed the more interesting antifungal activity, was selected and purified by reverse-phase high-pressure liquid chromatography. This complex is composed of four compounds. Ultraviolet-visible, infrared, mass, and 1H nuclear magnetic resonance spectroscopy studies showed that these molecules contain an aromatic ring substituted by aliphatic chains. These compounds differ from the known antibiotics produced by Actinomadura species.
Asunto(s)
Actinomycetales/metabolismo , Antiinfecciosos/metabolismo , Clima Desértico , Microbiología del Suelo , Actinomycetales/genética , Actinomycetales/ultraestructura , Argelia , Antibacterianos/análisis , Antibacterianos/biosíntesis , Antibacterianos/química , Antibacterianos/metabolismo , Antibacterianos/farmacología , Antiinfecciosos/química , Antiinfecciosos/farmacología , Antifúngicos/química , Antifúngicos/metabolismo , Antifúngicos/farmacología , Medios de Cultivo Condicionados/química , Hongos/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Concentración de Iones de Hidrógeno , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Microscopía Electrónica de Transmisión , Filogenia , ARN Ribosómico 16S/genética , TemperaturaRESUMEN
AIMS: To study the effect of sulfur-containing amino acids (L-cysteine, L-cystine, L-methionine and DL-ethionine) on the production of dithiolopyrrolone antibiotics by Saccharothrix algeriensis NRRL B-24137. METHODS AND RESULTS: The production levels of dithiolopyrrolones were investigated by using high performance liquid chromatography in a chemically semi-synthetic medium. The production of the studied antibiotics depends upon the nature, concentration and the time of addition of these sources in the culture medium. Both cysteine and cystine favoured the specific productions of dithiolopyrrolones; iso-butyryl-pyrrothine (ISP) by cysteine, however butanoyl-pyrrothine, senecioyl-pyrrothine and tigloyl-pyrrothine by cystine, when added initially to the culture medium. The maximum specific productions of dithiolopyrrolones were observed in the presence of 5 mmol l(-1) cystine for thiolutin, 5 mmol l(-1) cysteine for ISP, and 10 mmol l(-1) cystine for others studied dithiolopyrrolones as shown in Fig. 3. The production of these antibiotics was decreased when the concentrations of cysteine and cystine were in excess. All dithiolopyrrolone specific productions were strongly inhibited by addition of methionine and ethionine, without inhibition of mycelial growth. CONCLUSIONS: Among all studied amino acids, cystine and cysteine can be used as supplements for improvement the production of dithiolopyrrolone antibiotics by S. algeriensis NRRL B-24137. SIGNIFICANCE AND IMPACT OF THE STUDY: Dithiolopyrrolone antibiotics have many important applications for employing them as medicaments, particularly in the treatment of human and animal cancers. In the present work, the influence of containing-sulfur amino acids on dithiolopyrrolone antibiotic productions was studied. The obtained results can be employed for the optimization of the culture medium for the dithiolopyrrolone productions in higher quantities.
Asunto(s)
Actinomycetales/metabolismo , Aminoácidos Sulfúricos/farmacología , Antibacterianos/biosíntesis , Pirrolidinonas/metabolismo , Actinomycetales/efectos de los fármacos , Actinomycetales/crecimiento & desarrollo , Compuestos Bicíclicos Heterocíclicos con Puentes/metabolismo , Medios de Cultivo , Cisteína/farmacología , Cistina/farmacología , Relación Dosis-Respuesta a Droga , Etionina/farmacología , Fermentación , Concentración de Iones de Hidrógeno , Metionina/farmacologíaRESUMEN
Fortuitous growth of an actinomycete on Fusarium oxysporum f. sp. albedinis culture has shown a host-parasite process. As a response to the actinomycete, the fungus produces thallospores with various forms which can germinate faster than the non-parasited F. o. albedinis microconidies. However, the strains obtained from thallospores showed as sensible as the mother strain towards actinomycete action.
Asunto(s)
Actinomycetales/crecimiento & desarrollo , Fusarium/fisiología , Esporas Fúngicas/fisiologíaRESUMEN
Strains of Actinomycetales (286 in toto) belonging to thirteen genera other than Streptomyces were isolated from different ecosystems in the Algerian Sahara. Among these strains, 32 showed antibiotic activity against at least one of the test organisms comprising four bacteria, two yeasts and several filamentous fungi, and twelve were antagonistic against both bacteria and fungi. The characteristic UV spectra of active extracts revealed that six strains produced non-polyenic antifungal substances and four synthetized polyenic substances. One strain of Spirillospora produced a pentaene antifungal substance. The only antibiotic so far known to be produced by this genus is spirillomycin.
Asunto(s)
Actinomycetales/metabolismo , Antibacterianos/aislamiento & purificación , Evaluación Preclínica de Medicamentos , Actinomycetales/aislamiento & purificación , África del Norte , Antibacterianos/química , Antibacterianos/farmacología , Antifúngicos/química , Antifúngicos/aislamiento & purificación , Antifúngicos/farmacología , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Grampositivas/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Microbiología del Suelo , Especificidad de la Especie , Espectrofotometría Ultravioleta , Microbiología del Agua , Levaduras/efectos de los fármacosRESUMEN
The taxonomic position of a soil isolate, strain SA 233T, recovered from Saharan soil from Algeria was established using a polyphasic approach. This isolate has been previously reported to produce three novel dithiolopyrrolone antibiotics, and preliminary chemotaxonomic and morphological characteristics suggested that it was representative of a member of the genus Saccharothrix. Phylogenetic analysis of the strain from 16S rDNA sequences, along with a detailed analysis of morphological, chemotaxonomic and physiological characteristics, indicates that it belongs to the genus Saccharothrix and represents a novel species that is readily distinguished from all recognized Saccharothrix species. The name Saccharothrix algeriensis sp. nov. is proposed for the isolate, with type strain SA 233T (=NRRL B-24137T=DSM 44581T).