Asunto(s)
Antígenos de Neoplasias , Neoplasias del Colon/inmunología , Pólipos Intestinales/inmunología , Animales , Membrana Celular/inmunología , Epítopos , Técnica del Anticuerpo Fluorescente , Cabras/inmunología , Humanos , Sueros Inmunes , Mucosa Intestinal/inmunología , Microscopía Electrónica , Conejos/inmunologíaAsunto(s)
Adenocarcinoma/inmunología , Antígenos/análisis , Antígeno Carcinoembrionario/análisis , Reacciones Cruzadas , Sistema Digestivo/inmunología , Neoplasias Gastrointestinales/inmunología , Animales , Membrana Celular/inmunología , Colon/inmunología , Neoplasias del Colon/inmunología , Mucosa Gástrica/inmunología , Humanos , Sueros Inmunes , Técnicas Inmunológicas , Mucosa Intestinal/inmunología , Conejos/inmunología , Ovinos/inmunología , Neoplasias Gástricas/inmunologíaRESUMEN
The histological localization of CEA in gastrointestinal tissues was re-evaluated after absorption of anti-CEA antiserum with NCA2, another normal antigen cross-reacting with CEA. This absorbed antiserum showed clearly the presence of CEA in colonic tumors and some non-cancerous colonic mucosae, obtained even from non-cancerous patients. In contrast, some of the gastric adenocarcinomas we studied were strained very weakly by absorbed anti-CEA antiserum, although non-absorbed antiserum (or serum absorbed with NCA alone) labelled them strongly. The same difference in reactivity between both antisera was observed in intestinal metaplasia. The localization of NCA2 was studied with a specific antiserum, previously absorbed with CEA and NCA. NCA2 was found to be a noromal cytoplasmic and mucus-associated antigen of gastrointestinal tissues. It was present also in fetal stomach and colon. Sections of gastric and colonic tumors as well as intestinal metaplasia of gastric mucosae always reacted brilliantly with absorbed anti-NCA2 antiserum.
Asunto(s)
Antígenos , Antígeno Carcinoembrionario , Sistema Digestivo/inmunología , Neoplasias Gastrointestinales/inmunología , Antígenos/análisis , Antígeno Carcinoembrionario/análisis , Neoplasias del Colon/inmunología , Epítopos , Técnica del Anticuerpo Fluorescente , Mucosa Gástrica/inmunología , Humanos , Sueros Inmunes , Mucosa Intestinal/inmunología , Neoplasias del Recto/inmunología , Neoplasias Gástricas/inmunologíaRESUMEN
Crude extracts of colorectal surgical tumors, either individual or pooled, and HT29 line cells were compared in leucocyte migration inhibition test. HT29 cell extract gave more positive reactions with leucocytes of colorectal cancer patients than the other ones, and less with control leucocytes. It was thus used for all the subsequent experiments. As a whole, we obtained 44% (30/68) of positive inhibitions in patients afflicted with a colorectal carcinoma, against 9% (3/31e in normal controls and 20% (8/40) in patients bearing a carcinoma from another organ. Among the latest patients, those having a gynecological carcinoma were rarely positive, contrasting with those suffering from a carcinoma from the aerodigestive organs. It seemed that the antigen(s) involved in the reaction had no organ specificity, but rather a broad tissue specificity.
Asunto(s)
Inhibición de Migración Celular , Neoplasias del Colon/inmunología , Leucocitos/inmunología , Neoplasias del Recto/inmunología , Adenocarcinoma/inmunología , Línea Celular , Humanos , Técnicas In VitroRESUMEN
Leucocytes challenged with anti-NCA serum (non-specific cross-reacting antigen) can release histamine. This release is Ca++, glucose and temperature dependent as usually described for basophils. Only half of the subjects whose basophils can release histamine when challenged with anti-IgE serum are also able to release histamine with anti-NCA serum. There is no cross-reaction between IgE and anti-NCA serum or between NCA and anti-IgE serum. So it seems likely that NCA might be a marker on the surface of some human basophils.