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1.
Transfus Apher Sci ; 63(5): 103983, 2024 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-39098276

RESUMEN

Adequate stem cell harvesting is required for autologous hematopoietic transplantation. In deficient mobilizer patients, the collection of stem cells can be challenging because of the impossibility of achieving satisfactory CD34 cell counts with GCSF + - chemotherapy. Plerixafor is a potent and expensive drug that promotes the release of stem cells from the medullary niche to the peripheral blood and allows satisfactory harvests. We performed a retrospective analysis of 370 patients with myeloma and lymphoma harvested at our institution. 99 % of patients achieved satisfactory apheresis using Plerixafor in 45 %. Satisfactory harvests were obtained in patients mobilized with GCSF or plerixafor. In patients who used plerixafor, it was necessary to perform fewer apheresis procedures (P = 0.05). In multivariate analysis, the only factor that predicted the need for plerixafor was the presence of less than 30,000 CD34 / ul on the day of apheresis (OR 0.3. p < 0.001). Since we adopted the plerixafor protocol guided by CD34 counts, the number of patients with harvest failure has decreased. In conclusion, the rational and standardized use of plerixafor favors satisfactory harvest in patients who require autologous transplantation in South-American patients.


Asunto(s)
Eliminación de Componentes Sanguíneos , Trasplante Autólogo , Humanos , Femenino , Masculino , Eliminación de Componentes Sanguíneos/métodos , Persona de Mediana Edad , Trasplante Autólogo/métodos , Adulto , Estudios Retrospectivos , Trasplante de Células Madre Hematopoyéticas/métodos , Chile , Anciano , Ciclamas/farmacología , Ciclamas/uso terapéutico , Movilización de Célula Madre Hematopoyética/métodos , Bencilaminas
2.
Surg Endosc ; 35(4): 1915-1920, 2021 04.
Artículo en Inglés | MEDLINE | ID: mdl-33398579

RESUMEN

BACKGROUND: Percutaneous endoscopic gastrostomy (PEG) tube placement is one of the most common methods for establishing durable enteral access. Early PEG dislodgement occurs in < 5% of cases but typically prompts urgent surgical intervention to reestablish the gastrocutaneous tract and prevent intra-abdominal sepsis. To date, there is a single case report in the literature where successful endoscopic "rescue" of an early dislodged PEG tube negated the need for operative intervention. Here, we report our experience with a series of endoscopic PEG rescues for early dislodged PEG tubes. METHODS: A retrospective analysis of cases was reviewed from two institutions. Patients with early PEG dislodgements underwent PEG rescue using a gastroscope and standard Ponsky "Pull" PEG techniques through the original tract. RESULTS: Eleven patients were identified from the database and underwent PEG rescue after early PEG dislodgement. Mean operative time was 68 min, and there were no complications related to PEG rescue. PEG rescue permitted safe re-establishment of the gastrostomy tract while avoiding laparoscopic or open surgical intervention in hemodynamically stable patients. All patients tolerated the procedure well and were able to resume use of the PEG tubes shortly after intervention. CONCLUSION: Endoscopic rescue represents a feasible noninvasive option for PEG tube replacement following early inadvertent PEG tube dislodgement in appropriate clinical settings.


Asunto(s)
Endoscopía , Gastrostomía , Intubación Gastrointestinal , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Laparoscopía , Masculino , Persona de Mediana Edad , Peritoneo/cirugía , Estudios Retrospectivos , Estomas Quirúrgicos
3.
Ann Oncol ; 23(8): 2138-2146, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22228453

RESUMEN

BACKGROUND: The presence of genetic changes is a hallmark of chronic lymphocytic leukemia (CLL). The most common cytogenetic abnormalities with independent prognostic significance in CLL are 13q14, ATM and TP53 deletions and trisomy 12. However, CLL displays a great genetic and biological heterogeneity. The aim of this study was to analyze the genomic imbalances in CLL cytogenetic subsets from both genomic and gene expression perspectives to identify new recurrent alterations. PATIENTS AND METHODS: The genomic imbalances and expression levels of 67 patients were analyzed. The novel recurrent abnormalities detected with bacterial artificial chromosome array were confirmed by FISH and oligonucleotide microarrays. In all cases, gene expression profiling was assessed. RESULTS: Copy number alterations were identified in 75% of cases. Overall, the results confirmed FISH studies for the regions frequently involved in CLL and also defined a new recurrent gain on chromosome 20q13.12, in 19% (13/67) of the CLL patients. Oligonucleotide expression correlated with the regions of loss or gain of genomic material, suggesting that the changes in gene expression are related to alterations in copy number. CONCLUSION: Our study demonstrates the presence of a recurrent gain in 20q13.12 associated with overexpression of the genes located in this region, in CLL cytogenetic subgroups.


Asunto(s)
Aberraciones Cromosómicas , Cromosomas Humanos Par 20 , Leucemia Linfocítica Crónica de Células B/genética , Hibridación Genómica Comparativa , Dosificación de Gen , Perfilación de la Expresión Génica , Inestabilidad Genómica , Humanos , Hibridación Fluorescente in Situ , Leucemia Linfocítica Crónica de Células B/sangre
4.
Vet Parasitol Reg Stud Reports ; 21: 100429, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-32862909

RESUMEN

Coccidiosis of sheep is an intestinal infection caused by protozoa of the genus Eimeria. An outbreak of the disease in adult sheep from Salta province, northwestern Argentina, was studied to establish its clinical, epidemiological, pathological and etiological aspects. The affected animals were part of a flock of 20 sheep brought from Formosa province about 10 days before. Most sheep (80% incidence) showed hemorrhagic diarrhea, dehydration and loss of body condition; six of them died and two that became permanently recumbent were euthanized. Three necropsied sheep showed mild mesenteric lymphadenomegaly, diffuse proliferative enteritis in the small and large intestines, and mucosal thickening. Histopathological studies exhibited diffuse proliferative enteritis and presence of structures compatible with intracellular coccidia at different stages of development. Parasitological studies (n = 12) resulted in an average of 16,636.6 (± 15,266.8) Eimeria oocysts per gram of feces (range 1680-46,400). Taxonomy of Eimeria species based on analysis of sporulated oocysts derived from 4 fecal samples (n = 100 oocyst per sample) showed, on average, a high prevalence of E. ovinoidalis (61.5%), followed by E. parva (27.2%), and lower proportions of E. crandallis (5.3%), E. ahsata (3.2%) and E. intricata (2.8%). Clinical and pathological findings confirmed the diagnosis of coccidiosis in the affected sheep; parasitological results showed that E. ovinoidalis was the main species responsible for the clinical signs. Clinical coccidiosis is considered unusual in adult sheep, but the present case shows that under favorable environmental and/or management conditions, this infection may be highly deleterious for adult sheep.


Asunto(s)
Coccidiosis/veterinaria , Brotes de Enfermedades/veterinaria , Eimeria/aislamiento & purificación , Enfermedades de las Ovejas/patología , Animales , Argentina/epidemiología , Coccidiosis/epidemiología , Coccidiosis/parasitología , Coccidiosis/patología , Eimeria/clasificación , Prevalencia , Ovinos , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/parasitología , Oveja Doméstica
5.
Transfus Med ; 19(5): 260-8, 2009 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-19747289

RESUMEN

Idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder characterized by a low platelet count and bleeding, whose incidence is approximately 6.2 for each 100,000 adults per year. Intravenous immunoglobulins (IVIG) can be useful in patients with ITP to prevent bleeding or prior to surgery. In this study, the efficacy and safety of Flebogammadif, a new high-purity IVIG, were assessed by an open, multicentre, non-controlled, prospective study in adult patients with chronic ITP. A total of 20 patients (enrolled if experiencing chronic ITP since at least 6 months before recruitment and if platelet count <20 x 10(9)L(-1) before treatment) received 0.4 g kg(-1)-bw of Flebogammadif for 5 consecutive days and were followed-up for 3 months. Efficacy endpoints were three: proportion of patients who reached a platelet count > or = 50 x 10(9)L(-1), time for the platelet count to reach that level and duration of response. Safety parameters [adverse events (AE), laboratory determinations and vital signs] and viral markers were regularly monitored. A total of 14 patients achieved a platelet count of > or = 50 x 10(9)L(-1). The median time to platelet response was or = 50 x 10(9)L(-1) was > or = 7 days. A regression of haemorrhages was reported for 17 patients on day 14. Eight patients presented 21 AEs (mostly mild) potentially related to the study drug. Neither abnormalities in laboratory values nor in viral markers were registered during the follow-up period. Flebogammadif was well tolerated and succeeded in providing a haemostatic platelet count in patients with ITP.


Asunto(s)
Inmunoglobulinas Intravenosas/administración & dosificación , Púrpura Trombocitopénica Idiopática/tratamiento farmacológico , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Enfermedad Crónica , Efectos Colaterales y Reacciones Adversas Relacionados con Medicamentos , Hemorragia/inducido químicamente , Hemostasis , Humanos , Inmunoglobulinas Intravenosas/farmacología , Persona de Mediana Edad , Recuento de Plaquetas , Púrpura Trombocitopénica Idiopática/sangre , Púrpura Trombocitopénica Idiopática/complicaciones , Resultado del Tratamiento , Adulto Joven
6.
J Neurosci ; 21(4): 1413-9, 2001 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-11160413

RESUMEN

This laboratory has demonstrated that a single methamphetamine (METH) injection rapidly and reversibly decreases the activity of the dopamine transporter (DAT), as assessed ex vivo in synaptosomes prepared from treated rats. This decrease does not occur because of residual drug introduced by the original injection or nor is it associated with a change in binding of the DAT ligand WIN35428. The purpose of this study was to elucidate the mechanism or mechanisms of this METH effect by determining whether direct application of this stimulant to synaptosomes causes changes in DAT similar to those observed ex vivo. Similar to the ex vivo effect, incubation of striatal synaptosomes with METH decreased DAT activity, but not WIN35428 binding: the effect on activity was not eliminated by repeated washing of synaptosomes. Also, as observed ex vivo, incubation with 3,4-methylenedioxymethamphetamine, but not cocaine or methylphenidate, caused a METH-like reduction in DAT function. The rapid and reversible METH-induced diminution in DAT activity did not occur because of a change in membrane potential, as assessed in vitro and ex vivo by [(3)H]tetraphenylphosphonium accumulation. However, the METH-related decline in DAT function may be attributed to phosphorylation because NPC15437, a protein kinase C inhibitor, attenuated the METH-induced decline in DAT function. Similarities between previously reported effects ex vivo of a single METH injection on serotonin and norepinephrine transporter function and effects of direct METH application in vitro were also found. Together, these data demonstrate that the in vitro incubation model mimics the rapid and reversible effects observed after a single METH injection.


Asunto(s)
Proteínas Portadoras/metabolismo , Cocaína/análogos & derivados , Glicoproteínas de Membrana , Proteínas de Transporte de Membrana , Metanfetamina/farmacología , Proteínas del Tejido Nervioso , Sinaptosomas/efectos de los fármacos , Sinaptosomas/metabolismo , 3,4-Metilenodioxianfetamina/farmacología , Animales , Transporte Biológico/efectos de los fármacos , Cocaína/farmacología , Cuerpo Estriado/química , Dopamina/farmacocinética , Proteínas de Transporte de Dopamina a través de la Membrana Plasmática , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos/farmacología , Técnicas In Vitro , Masculino , Potenciales de la Membrana/efectos de los fármacos , Metanfetamina/síntesis química , Metilfenidato/farmacología , Modelos Biológicos , Norepinefrina/farmacocinética , Compuestos Onio/metabolismo , Compuestos Organofosforados/metabolismo , Fosforilación/efectos de los fármacos , Proteína Quinasa C/antagonistas & inhibidores , Ratas , Ratas Sprague-Dawley , Serotonina/farmacocinética , Sinaptosomas/química
7.
Eur J Pharmacol ; 409(3): 265-71, 2000 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-11108820

RESUMEN

Multiple methamphetamine administrations rapidly decrease rat striatal dopamine transporter activity. To determine the species specificity of this phenomenon, the present studies examined effects of this stimulant on the dopamine transporter in mice. As in rats, multiple methamphetamine injections rapidly reduced striatal dopamine transporter activity; a decrease that was partially reversed 24 h later. Moreover, methamphetamine decreased binding of the dopamine transporter ligand, WIN35428, but to a lesser degree than the change in dopamine transporter function. These decreases did not appear to result from residual methamphetamine introduced by the original drug treatment. As in rats, hyperthermia contributed to this phenomenon. Unlike in rats, a role for dopamine was not observed in mice as dopamine depletion, resulting from alpha-methyl-p-tyrosine pretreatment, did not prevent this decrease. In addition, unlike in rats, pretreatment with either a dopamine D1 or D2 receptor antagonist (SCH23390 or eticlopride, respectively) did not attenuate the methamphetamine-induced reduction in dopamine uptake. These findings demonstrate both similarities and differences in the acute effects of methamphetamine on dopamine transporter function in mice and rats, and suggest the mouse as an additional model for assessing the acute effects of methamphetamine on the dopamine transporter.


Asunto(s)
Cardiotónicos/farmacocinética , Proteínas Portadoras/efectos de los fármacos , Cuerpo Estriado/efectos de los fármacos , Dopaminérgicos/farmacología , Dopamina/farmacocinética , Fiebre/metabolismo , Glicoproteínas de Membrana , Proteínas de Transporte de Membrana , Metanfetamina/farmacología , Proteínas del Tejido Nervioso , Animales , Proteínas Portadoras/metabolismo , Cuerpo Estriado/metabolismo , Proteínas de Transporte de Dopamina a través de la Membrana Plasmática , Relación Dosis-Respuesta a Droga , Masculino , Ratones , Ratas
8.
Avian Dis ; 38(3): 672-8, 1994.
Artículo en Inglés | MEDLINE | ID: mdl-7832727

RESUMEN

Seventeen complicated outbreaks of infectious coryza in layer, broiler-breeder, and broiler flocks were studied. In the layer flock outbreaks, drops in egg production of up to 35% were seen. In the broiler flocks and several of the layer flocks, losses due to persistent mortality and/or culling varied between 2 and 5%. Signs of infectious coryza in both layers and broiler-breeders were typical; in broilers, however, swollen head-like syndrome was seen. Except in one flock, no viral diseases were clinically or serologically detected. Excluding broiler-breeders, birds from most other flocks were serologically positive for Mycoplasma gallisepticum, and some were also positive for M. synoviae. Haemophilus paragallinarum was isolated from all of the outbreaks, but only as a pure culture in three outbreaks. Isolation of H. paragallinarum from sites such as liver, kidney, and particularly tarsal arthritis and ocular globes appears to be reported for the first time. Serovar A was isolated in eight outbreaks, serovar B in six, serovar C in one, and untypable serovars in two. The severity of these infectious coryza outbreaks may have been increased by concurrent salmonellosis, pasteurellosis, and mycoplasmosis, although under certain conditions H. paragallinarum is able to cause septicemia. Ten of the outbreaks occurred in birds vaccinated against infectious coryza; this may be due to the use of vaccines that do not provide protection against the types of H. paragallinarum that affect poultry in the region.


Asunto(s)
Pollos , Brotes de Enfermedades/veterinaria , Infecciones por Haemophilus/veterinaria , Enfermedades de las Aves de Corral/epidemiología , Animales , Argentina/epidemiología , Brotes de Enfermedades/prevención & control , Femenino , Haemophilus/clasificación , Haemophilus/aislamiento & purificación , Infecciones por Haemophilus/epidemiología , Infecciones por Haemophilus/prevención & control , Masculino , Infecciones por Pasteurella/complicaciones , Infecciones por Pasteurella/veterinaria , Enfermedades de las Aves de Corral/prevención & control , Salmonelosis Animal/complicaciones , Serotipificación
9.
Avian Dis ; 37(2): 310-4, 1993.
Artículo en Inglés | MEDLINE | ID: mdl-8363497

RESUMEN

The biochemical and serological properties of Haemophilus paragallinarum isolates recovered from 11 recent outbreaks of infectious coryza in layer hens and one case of swollen-head syndrome in broilers in Argentina are described. Twenty-four isolates had the typical biochemical properties of H. paragallinarum. All isolates were serotyped according to the Page scheme. Ten of the isolates were serovar A, 11 were serovar B, one was serovar C, and two isolates could not be serotyped. The isolates were also examined using a panel of monoclonal antibodies (MAbs) for Page serovars A (one MAb available) and C (three MAbs available). The serovar B isolates all failed to react with any MAb. The serovar C isolate reacted with all three serovar C MAbs but not with the serovar A MAb. Only six of the 10 serovar A isolates reacted with the serovar A MAb. These results indicate that H. paragallinarum isolates from Argentina are antigenically distinct from those examined in other countries, and it is suggested that coryza vaccines intended for use in Argentina may be more effective if based on local strains.


Asunto(s)
Pollos/microbiología , Brotes de Enfermedades/veterinaria , Infecciones por Haemophilus/veterinaria , Enfermedades de las Aves de Corral/microbiología , Animales , Argentina/epidemiología , Haemophilus/clasificación , Haemophilus/citología , Haemophilus/metabolismo , Infecciones por Haemophilus/epidemiología , Infecciones por Haemophilus/microbiología , Enfermedades de las Aves de Corral/epidemiología , Serotipificación/veterinaria , Especificidad de la Especie
10.
Avian Dis ; 38(2): 361-5, 1994.
Artículo en Inglés | MEDLINE | ID: mdl-7980289

RESUMEN

Two monoclonal antibodies (MAbs) raised against a serovar A Haemophilus paragallinarum were evaluated for their ability to react with 11 reference strains that represented all the recognized serovars and with 27 field isolates of Page serovar A collected from around the world. The MAbs were used in a hemagglutination-inhibition assay. Both MAbs recognized type strains of Page serovar A and Kume serovars A-1 and A-2 but not the type strains of Kume serovars A-3 and A-4. Neither MAb recognized the type strains of Page serovars B and C or Kume serovars B-1, C-1, C-2, C-3, or C-4. When evaluated with the 27 Page serovar A field isolates, both MAbs recognized only 10 isolates. All of the recognized isolates belonged to Kume serovars A-1 (nine isolates) or A-2 (one isolate). All of the field isolates that were not recognized by one or the other of the MAbs either were Kume serovar A-4 (seven isolates) or could not be placed in an existing Kume A serovar (10 isolates). The results indicate that the epitope recognized by these MAbs is present only in strains of H. paragallinarum that belong to Kume serovars A-1 and A-2.


Asunto(s)
Anticuerpos Monoclonales , Haemophilus/inmunología , Serotipificación/métodos , Animales , Pollos , Reacciones Cruzadas , Haemophilus/clasificación , Haemophilus/aislamiento & purificación , Pruebas de Inhibición de Hemaglutinación , Pruebas de Hemaglutinación
11.
Med Clin (Barc) ; 116(9): 339-42, 2001 Mar 10.
Artículo en Español | MEDLINE | ID: mdl-11333766

RESUMEN

BACKGROUND: To analyse the results of the treatment with 2-chlorodeoxyadenosine (2CdA) in 9 patients with refractory or relapsed Langerhans cell histiocytosis (LCH) tracted in 8 Spanish hospitals between 1993 and 1999. PATIENTS AND METHOD: In the 9 patients the following data were recorded: age, sex, organ involvement by LCH, first treatment and response, dose, number of cycles and schedule of administration 2CdA, response to 2CdA treatment, toxicity, disease-free survival (DFS) and overall survival (OS). RESULTS: Median age was 25 years (range, 6-63). All patients had multiorganic involvement by LCH, with severe organ dysfunction in 4. 2CdA was administered as second line treatment in 7 cases and as third line in 2. The 2CdA dose was 0.1 mg/kg per day during 5 days in the majority of patients, and interval between cycles was 4 weeks. In 2 cases a complete remission (CR) was achieved and 4 cases attained a partial remission (PR) (overall response rate 66%). The main toxicity was hematologic, with neutropenia grade > 2 in 5 cases and thrombocytopenia > 2 in 5. Four patients had infections, with fatal evolution in one of them. After a median follow-up of 8 months (range 2-17), 2 patients remained in CR (12 months both), 4 in PR (range 2-12 months) and one had active disease (17 months). The other two died due to progressive disease and Aspergillus spp. sepsis, respectively. The actuarial probabilities of DFS and OS were 58% (95% CI, 38-78%) and 71% (95% CI, 54-88%), respectively. CONCLUSIONS: 2CdA is an active drug for patients with refractory or relapsed LCH, and its main toxicity is myelosuppression. The usefulness of 2CdA, isolated or in combination with other drugs, in patients with refractory or relapsed LCH must be assessed in controlled studies.


Asunto(s)
Cladribina/uso terapéutico , Histiocitosis de Células de Langerhans/tratamiento farmacológico , Inmunosupresores/uso terapéutico , Adolescente , Adulto , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Recurrencia
12.
J Dent Educ ; 48(12): 669-73, 1984 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-6209316

RESUMEN

To reduce repetitive endodontic errors by senior dental students, a formal review program was established. It consisted of a slide-tape and a written, latent-image simulation of an endodontic case requiring diagnostic and treatment decisions. Before undertaking any endodontic cases in the senior year, each student had to complete the program successfully. The program significantly reduced diagnostic errors in six categories for posterior teeth and in four of six categories for anterior teeth. Errors were also significantly reduced in eight of 12 treatment categories for posterior teeth and in five of 12 categories for anterior teeth. Students' opinions of the endodontic review program were quite favorable.


Asunto(s)
Educación en Odontología , Endodoncia/educación , Recursos Audiovisuales , Enfermedades de la Pulpa Dental/diagnóstico , Enfermedades de la Pulpa Dental/terapia , Errores Diagnósticos , Humanos , Proyectos Piloto
13.
J Dent Educ ; 51(9): 532-8, 1987 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-2442230

RESUMEN

The effects of four different endodontic self-instructional review formats (slide-tape, latent-image simulation, computer text simulation, and computer-assisted video interactive simulation) on senior clinical endodontic performance are compared. The senior class of 105 students was divided into eight groups of 13-14 academically equivalent students prior to initiating endodontic treatment in the 1985-86 academic year. With the exception of the control group, each participated in a program designed to review principles of endodontic diagnosis and treatment. All clinical endodontic cases were subsequently critiqued by a calibrated panel of two general dentist and one endodontist. Overall, students in the study group made fewer diagnostic errors compared with those in the control group, while the error reduction in clinical treatment was not significantly reduced between the two groups. No significant difference in error reduction was noted between the simulation formats. Student evaluations, as well as comparative developmental expenditures, are discussed.


Asunto(s)
Simulación por Computador , Instrucción por Computador , Endodoncia/educación , Actitud , Recursos Audiovisuales , Competencia Clínica , Evaluación Educacional , Estudios de Evaluación como Asunto , Humanos , Estudiantes de Odontología
14.
Rev Argent Microbiol ; 30(2): 84-92, 1998.
Artículo en Español | MEDLINE | ID: mdl-9744035

RESUMEN

Two groups of 6 laying hens were used to produce IgY. In the vaccinated group (V), hens were injected by intramuscular route with two doses of a Salmonella enterica serovar Enteritidis bacterin at 20-day interval. In the control group (T) hens remained unvaccinated. Four IgY extractions were performed on the egg production of both groups. The first two extractions were carried out using the yolks obtained from the eggs produced during the 4th and 5th post-vaccination week (extracts 1V and 1T) and the other two using the ones from the 6th, 7th and 8th week (2V and 2T). Starting from the extracts 1V and 1T other products were obtained by freezing-thawing (1V-A and 1T-A) and simple (1V-B and 1T-B) or double (1V-C and 1T-C) flow capillary dialysis concentration. All these products were compared using an ELISA test specific for the detection of chicken antibodies against flagellar antigens of S. Enteritidis. In this test, V extracts were positive whereas T extracts were negative. The extract 1V was more positive than the extract 2V. The extract 1V-C was the most positive and was therefore selected to be used as an antiserum in the agglutination tests. This extract contained 1.9 g/dl of total proteins, 0.028 g/dl of triglycerides and 0.012 g/dl of cholesterol and showed an electrophoretic pattern characteristic of IgY. The 1T-C extract was used as a negative control in the agglutination tests. Slide somatic and tube flagellar agglutination tests were simultaneously carried out using both IgY extracts and a standard rabbit anti-Salmonella (IgG) sera. Overall 367 strains from the Enterobacteriaceae family were tested together with two other strains belonging to the Vibrionaceae family. The 1V-C extract specifically agglutinated S. Enteritidis strains in the same way as the rabbit sera. This extract also agglutinated other Salmonella strains antigenically related to S. Enteritidis. Salmonella which did not share somatic or flagellar antigens with S. Enteritidis, other different species of the Enterobacteriaceae family and the two strains of the Vibrionaceae family were all negative. None of the strains tested was agglutinated by the 1T-C extract. This paper show that it is possible to use specific IgY to identify S. enterica serovars. The more extended use of IgY for diagnostic purposes may be a convenient way to complement the current use of mammal polyclonal antibodies.


Asunto(s)
Pruebas de Aglutinación , Anticuerpos Antibacterianos/inmunología , Vacunas Bacterianas/inmunología , Pollos/inmunología , Proteínas del Huevo/inmunología , Inmunoglobulinas/inmunología , Enfermedades de las Aves de Corral/diagnóstico , Salmonelosis Animal/diagnóstico , Salmonella enterica/inmunología , Animales , Enterobacteriaceae/inmunología , Femenino , Inyecciones Intramusculares , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/prevención & control , Conejos , Salmonelosis Animal/inmunología , Salmonelosis Animal/prevención & control , Salmonella enterica/clasificación , Serotipificación , Especificidad de la Especie , Vacunación/veterinaria , Vibrionaceae/inmunología
15.
Tex Dent J ; 110(5): 13-6, 1993 May.
Artículo en Inglés | MEDLINE | ID: mdl-8211813

RESUMEN

The purpose of this manuscript is to review the history, indications, and contraindications of nitrous oxide/oxygen as a conscious sedative agent. Included is a discussion of its addictive potential and the implications of its utilization with individuals who are recovering from addictive diseases. Additional discussion is devoted to the myeloneuropathies resulting from sustained "recreational" use. Particular attention is directed to dental personnel, the population with the greatest risk for addiction.


Asunto(s)
Óxido Nitroso , Trastornos Relacionados con Sustancias , Humanos , Legislación de Medicamentos
16.
Rev. otorrinolaringol. cir. cabeza cuello ; 77(2): 117-123, jun. 2017. ilus
Artículo en Español | LILACS | ID: biblio-902751

RESUMEN

Introducción: La detección precoz de hipoacusia permanente en lactantes beneficia el desarrollo integral del paciente. Los programas cuyo objetivo es la identificación universal de hipoacusia debieran tener como meta determinados criterios de calidad en su ejecución. Objetivo: El objetivo del presente trabajo es comunicar los resultados del Programa de Detección Precoz de Hipoacusia en el Hospital Padre Hurtado. Material y método: Se incluyen los recién nacidos entre el 1 de enero de 2014 y el 31 de agosto de 2016. Los pacientes sin factores de riesgo para hipoacusia congénita se evalúan con examen de emisiones otoacústicas, y los pacientes con factores de riesgo con potenciales auditivos automatizados de tronco encefálico. Refieren aquellos pacientes con exámenes alterados en forma uní o bilateral. La etapa diagnóstica incluye potenciales auditivos evocados con tono, impedanciometría de alta frecuencia y audiometría de refuerzo visual. Los pacientes con diagnóstico de hipoacusia permanente son amplificados e inician proceso de habilitación. Resultados: En el período de estudio el universo a evaluar fue de 12.313 recién nacidos. Se completó la etapa de pesquisa en 98.4% con una tasa de referencia de 0.6%. 79 pacientes pasaron a etapa diagnóstica, completaron su evaluación antes de 3 meses en 95% de los casos. Se confirmó hipoacusia sensorioneural en 7 casos, con una tasa de 0.56 por 1.000 recién nacidos vivos. En 57% de los pacientes se amplificaron antes de los seis meses de vida. Conclusiones: El Programa de Hipoacusia Congénita del Hospital Padre Hurtado cumple con los indicadores de calidad recomendados en los ítemes de pesquisa y diagnóstico. En la etapa de habilitación con audffonos esto se realiza antes de los seis meses de vida sólo en 57% de los casos.


Introduction: Quality indicators of the newborn hearing screening program in Hospital Padre Hurtado. Aim: Asses the accomplishment of quality indicators of the newborn hearing screening program in Hospital Padre Hurtado, Chile, as proposed by the Joint Committee on Infant Hearing Loss (JCIH). Material and method: Two stage screening protocol: otoacoustic emissions for babies in the well-infant nursery and automated auditory brainstem responses for those in the intensive care unit orwith risk factors. If they fail one or both ears they proceed to a comprehensive audiological assessment. Results: 12.313 live births between 01/01/2014 and 108/31/16, 12.103 were screened before discharge (98.4%). 79 cases proceeded to diagnostic assessment, referral rate 0.6%. 95% infants completed audiological evaluation before three months, seven cases were diagnose with permanent sensorineural hearing loss for a prevalence of 0.56 per 1000 live births. Amplification was provided before 6 months of age in 57% of deaf children. Conclusions: Quality indicators of the JCIH are met by our newborn hearing screening program with the exception of adequate timing for the provision of hearing aids: 57% before six months of age.


Asunto(s)
Humanos , Masculino , Femenino , Recién Nacido , Evaluación de Programas y Proyectos de Salud , Tamizaje Neonatal , Potenciales Evocados Auditivos , Pérdida Auditiva/diagnóstico , Calidad de la Atención de Salud , Estudios de Seguimiento , Diagnóstico Precoz , Pérdida Auditiva/congénito
17.
Rev. guatemalteca cir ; 21(1): 22-28, 2015. tab
Artículo en Español | LILACS | ID: biblio-869917

RESUMEN

Introducción: La residencia médica es un período de riesgo para el desarrollo de problemas de salud mental. Los estudios de ansiedad en residentesson contrastantes; sin embargo, se cree que los niveles de ansiedad son altos ya que los programas se desarrollan en hospitales nacionales que carecende recursos fsicos, materiales y humanos. El objetvo del presente estudio es determinar los niveles de ansiedad en médicos residentes en diferentesespecialidades empleando el cuestonario inventario de la ansiedad rasgo-estado (IDARE).Métodos: Se administró una encuesta electrónica para determinar el nivel de ansiedad en médicos residentes en diferentes especialidades del HospitalGeneral San Juan de Dios.Resultados: Se obtuvieron 132 encuestas para el análisis. La mayoría de los médicos encuestados son hombres (59%), solteros (83%) que no tenendependientes (79%), cursan residencia de cirugía o medicina interna (54%), trabajan más de 80 horas por semana (85%) y viajan menos de una horapara llegar al lugar de residencia (67%). El promedio del nivel de ansiedad-estado de los médicos residentes fue 46.94 puntos, mientras que el nivelpromedio de ansiedad-rasgo fue de 42.77 puntos. El 35% de los residentes poseen niveles de ansiedad medio y 59% altos en el momento de responderla encuesta; y el 56% posee niveles de ansiedad medio y 37% alto generalmente. No se encontró diferencia estadístcamente signifcatva en los nivelesde ansiedad en base a género, estado civil, tpo de residencia, dependientes y horas de tráfco. La única variable asociada con ansiedad fue la cantdadde horas de trabajo por semana.Conclusiones: La mayoría de los residentes encuestados poseen niveles de ansiedad medio-altos, la única variable asociada con niveles de ansiedadfue la cantdad de horas de trabajo por semana.


Background: Medical residency is a risky period for the development of mental health problems. Although anxiety studies in medical residents areinconclusive; we hypothesize anxiety levels in residents are high, since residency programs are developed in community hospitals that lack material andhuman resources. The aim of this study is to determine the levels of anxiety in medical residents of diferent specialtes using the State Trait-AnxietyInventary (STAI).Methods: An electronic poll with STAI was administered to medical residents of diferent specialtes of a tertary referral hospital.Results: We received 132 questonnaires for analysis. Most of the respondents are men (59%), single (83%), without dependents (79%), belong tosurgical or internal medicine residency (54%), work more than 80 hours per week (85%) and travel less than an hour to get to the hospital (67%). Theaverage level of state anxiety of the residents was 46.94 points, while the average level of trait anxiety was 42.77 points. Thirty fve percent of theresidents have medium anxiety levels and 59% high anxiety levels at the tme they answered the inventory; and 56% have medium anxiety levels and37% have high anxiety levels as a trait. We did not found a statstcal diference in anxiety levels based on gender, marital status, and type of residency,number of dependents or number of hours spent in trafc. The only variable that was associated with anxiety was the amount of work hours per week.Conclusions: Most of the residents have medium-high levels of anxiety; the only variable associated with anxiety levels was the amount of work hoursper week


Asunto(s)
Humanos , Agotamiento Profesional/diagnóstico , Ansiedad/diagnóstico , Internado y Residencia
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