Lectin-binding sites on the normal and pathologic uterus of sows.

The lectin-binding pattern was compared in the normal and pathological uterus of sows during the ovarian cycle. The following biotinylated lectins were used: Con A, DBA, SBA, PNA, RCA-I, UEA-I and WGA. Glycoconjugate labelling showed differences between phases of ovarian cycle and presence of morphologic lesions. Cystic endometrial hyperplasia increased the RCA-I reaction in the apical region of the glandular epithelium. There was higher intensity of labelling of WGA in the glandular epithelium in uteri with endometritis. In addition, increased Con A binding in the glandular epithelium and mild reduction of UEA-I reactivity in the glycocalyx of the glandular epithelium were detected in the cases of endometritis. The results of this study show that morphologic alterations modify the sugar pattern in the porcine uterus. These modifications in glycoconjugates may be one of the reasons for decreased fertility in sows.

Oral Lesions in Dogs with Visceral Leishmaniosis.

Canine visceral leishmaniosis (CVL) is an important zoonotic disease, which is endemic in Brazil and several other parts of the world. The disease may affect multiple organs, but lesions in the oral cavity are considered uncommon. Twenty-three cases of oral lesions compatible with CVL were diagnosed from 2015 to 2018 in the Federal District, Brazil. Six cases were confirmed to be CVL based on histopathology, immunohistochemistry and polymerase chain reaction amplification of Leishmania infantum kDNA. Most of the affected dogs were >9 years of age, of mixed breed and were classified as having stage C of the disease. The most important gross findings were ulcerated nodular lesions in the tongue, lip, gingiva and hard palate. Microscopically, there was lymphoplasmacytic to granulomatous inflammation that was diffuse, moderate to severe and associated with ulceration. CVL should be considered in the differential diagnosis of nodular and ulcerative oral lesions in dogs.

Microstructure and Hardness of Buffalo's Hoofs.

The aim of this study was to describe the microstructure of hoof capsules of the buffalo. In addition, the study emphasized the morphometric aspects of the horn tubules, the Vickers nanohardness of the dorsal and abaxial walls and sole of the digits of the thoracic and pelvic limbs of the buffalo. The abaxial wall in the thoracic and pelvic digits showed larger diameter of the horn tubules when compared to all dorsal wall and sole. In addition, the abaxial wall of the thoracic digits showed larger diameter of the horn tubules when compared with the pelvic digits. According to the three-dimensional microtomography, the dorsal wall was higher in density compared with the abaxial wall. The latter exhibited an intermediate density, while the sole showed the lowest density. The Vickers nanohardness test showed that there was no difference in hardness and resistance between the experienced regions. However, the elastic modulus was greater on the transversal section of the hoof capsule. In conclusion, the results of the current study show that modern technologies such as microtomography and subsequent imaging can be used to investigate details of the basic morphology in different regions of the buffalo's hoof.

Microtomographic Parameters and Nanoindentation of the Hoof of Girolando Cattle.

The aim of this study was to describe the microstructure of the pigmented and depigmented hoof capsule of Girolando cattle by bi- and tridimensional microtomography and nanoindentation, analysing the possible relation between these findings and the susceptibility of such animals to podal diseases. To carry out the microtomography and the nanoindentation, duplicated samples were collected from the dorsal wall, abaxial wall and pre-bulbar sole of the hoof capsule. Material collection was performed in 40 medial digits of thoracic limbs and 40 lateral digits of pelvic limbs. The bidimensional microtomography showed that the dorsal wall of the thoracic and pelvic limbs presented higher density, followed by the abaxial wall, and finally by the sole, with the lowest density. Moreover, the hoof capsule of cows of Girolando breed is a compact, non-porous material, and constituted by extratubular and intratubular keratin. By the tridimensional microtomography, it was possible to measure the angles of the corneal tubules in relation to the periople and the claws in the different regions of the hoof capsule, which were 90° for the dorsal wall, 55° for the abaxial wall and 70° for the sole. The tridimensional microtomography also showed corneal tubules of different diameters: 17, 51, 85, 119 and 153 µm. The nanoindentation test, when performed in different regions of the hoof capsule, did not reveal significant difference of Vickers hardness in the evaluated areas. However, we verified a larger elastic module of these regions on the transversal cut of the corneal tubules compared to the longitudinal cut.

Sistema de grupos sanguíneos AB em felídeos neotropicais e compatibilidade com gatos domésticos / AB blood group system in neotropical felids and compatibility with domestic cats

O principal sistema de grupos sanguíneos reconhecido para gatos é o AB. Os felinos apresentam anticorpos naturais contra o antígeno do tipo sanguíneo a que não pertencem, o que torna os testes de compatibilidade e as tipagens sanguíneas importantes na prevenção de reações transfusionais. O objetivo deste estudo foi realizar a tipagem sanguínea de oito gatos-mouriscos (Puma yagouaroundi), oito jaguatiricas (Leopardus pardalis), sete gatos-palheiros (Leopardus colocolo), sete gatos domésticos (Felis catus) da raça Persa e oito gatos domésticos sem raça definida (SRD), bem como realizar testes de compatibilidade entre os tipos sanguíneos iguais das diferentes espécies, para avaliar a possibilidade de transfusões interespecíficas. A técnica empregada para a tipagem foi a hemaglutinação em tubos de ensaio. A ocorrência do tipo sanguíneo tipo A foi de 100% entre as jaguatiricas, os gatos-palheiros e os gatos Persas e de 85,72% entre os gatos SRD. A ocorrência do tipo B foi de 100% nos gatos-mouriscos e de 14,28% nos gatos SRD. Considerando os testes de compatibilidade sanguínea, 87,5% (n=4) das jaguatiricas foram incompatíveis com os gatos domésticos, 100% (n= 6) dos gatos-palheiros foram compatíveis com os gatos domésticos e 100% (n= 4) dos gatos-mouriscos foram incompatíveis com os gatos domésticos do tipo B.(AU)

The blood group system recognized for cats is AB. Antibodies against other blood types occur naturally in cats, which makes the compatibility tests and blood typing important for preventing transfusion reactions. Wild felids need blood transfusions in cases of diseases and when run over on highways. The aim of this study was to perform blood typing of eight jaguarundies (Puma yagouaroundi), eight ocelots (Leopardus pardalis), seven pampas cats (Leopardus colocolo), seven domestic cats (Felis catus) of Persian breed and eight non-pedigree domestic cats (Felis catus), and test compatibility among the different species with the same blood types, to evaluate the possibility of performing interspecific blood transfusions. We conducted the study from August to December. We used haemagglutination in test tubes for typing. The occurrence of blood type A was 100% among ocelots, pampas cats and domestic cats of Persian breed, while non-pedigree domestic cats showed 85.72%. The occurrence of type B was 100% for jaguarundis and 14.28% for non-pedigree domestic cats. Regarding blood compatibility tests, 87.5% (n= 4) of the ocelots were incompatible with domestic cats; 100% (n=6) of the pampas cats were compatible with domestic cats, while 100% (n=4) of the jaguarundis were incompatible with type B domestic cats.(AU)

Hematimetria manual e automática em jaguatiricas (Leopardus pardalis - Linnaeus, 1758) / Manual and automated blood cells count in ocelots (Leopardus pardalis - Linnaeus, 1758)

Aparelhos de automação, cujo uso está estabelecido na hematologia dos animais domésticos, ainda não são empregados rotineiramente em felídeos selvagens. Esse estudo objetivou avaliar a técnica automatizada na contagem de células sanguíneas de jaguatiricas (Leopardus pardalis), comparando-a com a técnica manual. Foram coletadas amostras sanguíneas de oito jaguatiricas, que se submeteram à contagem de eritrócitos, leucócitos, plaquetas e à determinação do volume globular pelo método manual padrão e por meio do aparelho BC - 2800 VET® com a configuração para gatos domésticos. Os resultados foram avaliados por meio do teste t para dados pareados, e as técnicas submetidas à análise de correlação de Pearson. A técnica automatizada demonstrou resultados estatisticamente semelhantes para eritrócitos e leucócitos. Apenas para volume globular houve diferença significativa entre as técnicas manual e automatizada, mas a correlação foi alta. Apesar de não haver diferença significativa entre as técnicas para plaquetas, a correlação foi baixa. Conclui-se que o aparelho BC - 2800 VET® com a configuração para gatos domésticos é uma técnica confiável na realização do eritrograma e do leucograma para jaguatiricas. Para a determinação do parâmetro volume globular, o aparelho pode ser utilizado, desde que se faça a correção. Para a contagem de plaquetas, a técnica manual é recomendada.(AU)

Automated equipment, whose use is established in hematology of domestic animals, is not yet routinely used in blood cells count of wildlife, due to lack of studies that validate its use. The purpose of this study was to evaluate the automated technique for blood cells count of ocelots (Leopardus pardalis), comparing it with the manual technique. Blood samples were collected from eight ocelots, which were submitted to counting of erythrocytes, leukocytes, platelets and packed cell volume by the standard manual method and by the device VET ® 2800 BC with the configuration for domestic cats. The results were evaluated using the t test for paired data and the techniques submitted to Pearson correlation. The automated technique showed statistically similar results to erythrocytes and leukocytes. Significant difference was found only for packed cell volume between the manual and automated techniques, but the correlation was high. Although there was no significant difference between the techniques for platelets, the correlation was low. We concluded that the 2800 BC VET ® device with the configuration for domestic cats is a reliable technique in performing the erythrocyte and leukocyte counts for ocelots. The device may be used to determine packed cell volume, provided the correction is made. The manual technique is recommended for the platelet count.(AU)

Histological and morphometric characteristics of the corneal layer of buffalo hoof capsules / Características histológicas y morfométricas de la capa cornea de las cápsulas del casco de búfalo

SUMMARY: This study aimed to evaluate the microstructure of the buffalo's hoof capsule using hystomorphometry and computerized light microscopy. The length, thickness, gap and number of epidermal papillae and the morphology of the corneal tubules of the hoof were described in this paper. This study used 56 hoof capsules from hooves of 14 adults - age ranging from 24 to 60 months old - female buffaloes of the Jafarabadi breed; weighing around 650 kg. Fifty-six samples, 28 from thoracic limb and 28 from pelvic limbs, were analyzed, resulting in a total of 112 digits. Clinical specimens were collected at the coronary corium, laminar corium of the abaxial wall and pre-bulbar soles. The study concluded that the microstructure of the hoof capsule of buffalo hooves are made of epidermal papillae measuring 1,721.59 mm of length, 62.94 mm of thickness and 49.02 mm of gap between the papillae. This study determines that the coronary corium has more epidermal papillae than the laminar corium of the abaxial wall and pre-bulbar soles. The corneal tubules of the hoof capsules exhibit helical fashion and points to the possibility of applying this feature to the hooves of the biungulate species as well. In this research, we have been able to determine morphological parameters not yet described in scientific literature. These findings can be used in future comparative studies of healthy cattle and buffaloes and speculate evidence about the hooves' vulnerability facing different hoof diseases.

RESUMEN: El objetivo del estudio fue evaluar la microestructura de la cápsula del casco del búfalo mediante histomorfometría y microscopía óptica computarizada. Se describe la longitud, el espesor, el espacio y el número de papilas epidérmicas y la morfología de los túbulos corneales del casco. Utilizamos 56 cápsulas de cascos de 14 hembras adultas entre 24 a 60 meses de edad de la raza Jafarabadi, cuyo peso aproximado era de 650 kg. Analizamos 56 cascos, 28 del miembro torácico y 28 del miembro pélvico, con un total de 112 dedos. Se recogieron especímenes clínicos en el corion coronario, corion laminar de la pared abaxial y suelas pre-bulbares. Concluimos que la microestructura de la cápsula de los cascos de los búfalos está formada por papilas epidérmicas de 1.721,59 mm de longitud, 62,94 mm de espesor y 49,02 mm de espacio entre papilas. Se determinó que el corion coronario tiene más papilas epidérmicas que el corion laminar de la pared axial y las suelas pre-bulbares. Los túbulos córneos de las cápsulas de casco presentan una forma helicoidal y existe la posibilidad de que esta característica también se aplique a las pezuñas de otras especies de animales biungulados. También se determinaron algunos parámetros morfológicos no descritos en la literatura científica. Estos hallazgos pueden ser útiles en estudios comparativos de ganado bovino y bubalino saludable y en consideración de la 'vulnerabilidad' frente a diferentes enfermedades del casco.

Visceral Marek's disease in white-peafowl (Pavo cristatus) / Doença de Marek visceral em pavão-branco (Pavo cristatus)

Marek's disease (MD) is a lymphoproliferative disorder caused by Gallid herpesvirus 2 (MDV) that infects mainly domestic gallinaceous birds although wild birds may occasionally be affected. The current report describes the anatomopathological and molecular findings of a case of MD in a white-peafowl (Pavo cristatus). The signs included apathy, hyporexia, and diarrhea. Grossly, 0.5 to 1.5cm in diameter, yellow, soft nodules were observed in the skeletal muscle, lung, kidney, air sacs, small intestine, heart, ovary, ventriculus, and proventriculus. Microscopically, numerous atypical round neoplastic cells were noted. The molecular detection of MDV DNA was implemented to amplify part of the meq gene and products were sequenced for the phylogenetic analysis. Template DNA was obtained from tissues of the affected bird and from blood of all the gallinaceous birds of the Zoo. The expected amplicon for the partial amplification of MDV meq gene was obtained and the amplicons were sequenced. Sequences obtained enabled grouping the strain (accession no. KT768121) with MDV serotype 1 strains from the GenBank. Based on the anatomopathological and molecular findings, the diagnosis of MD in a white-peafowl was reached, and to the authors' knowledge, no previous report regarding MD was published in Pavo cristatus.(AU)

Doença de Marek (MD) é uma desordem linfoproliferativa causada pelo Gallid herpesvirus 2 (MDV), que infecta principalmente galináceos domésticos, porém aves silvestres podem ser ocasionalmente afetadas. O presente relato descreve os achados anatomopatológicos e moleculares de um caso de MD em um pavão-branco (Pavo cristatus). Os sinais clínicos incluíram apatia, hiporexia e diarreia. Macroscopicamente, foram observados nódulos macios, de 0,5 a 1,5cm de diâmetro, no músculo esquelético, no pulmão, nos rins, nos sacos aéreos, no intestino delgado, no coração, no ovário, no ventrículo e no proventrículo. Microscopicamente, numerosas células redondas neoplásicas atípicas foram notadas. A detecção molecular do DNA do MDV foi implementada para amplificar parte do gene meq, e os produtos foram sequenciados para análise filogenética. DNA foi obtido de tecidos de aves afetadas e do sangue de todos os galináceos do zoológico. A esperada amplificação de parte do gene meq de MDV amplificado foi ampliada e sequenciada. As sequências obtidas permitiram o agrupamento da cepa (acesso KT768121) com cepas do sorotipo 1 de MDV do GenBank.. O diagnóstico de MD em pavão-branco foi obtido com base nos achados anatomopatológicos e moleculares e, pelo conhecimento dos autores, não há relatos anteriores publicados de MD em Pavo cristatus.(AU)

Histomorphological evaluation of the digital coronary region at different fetal development stages of Holstein cattle / Avaliação histomorfológica da região coronária digital em diferentes fases de desenvolvimento fetal de bovinos da raça Holandesa

The scientific literature lacks detailed morphological descriptions of the histological development and cell differentiation of fetal bovine hoof. In this study, 40 extremity members of Holstein bovine fetuses were collected and divided into four groups (G1 to G4) based on the estimated age. Fragments were removed from wall and sole, processed and stained with hematoxylin - eosin (HE) for light microscopy observation. In G1, it was found that the epidermis was very thin, including keratinocyte layers and clusters of mesenchymal cells. In group G2 it was observed that the thickness of the epidermis covering the limbs remained variable and laminar corium developed in the germinal layer. In group G3 it was noted that in the germinal epithelium there were papillae in little advanced development and cells of the stratum corneum in the initial process of keratinization. In G4, the epidermis was well developed with layers distributed homogeneously, containing symmetrical and long papillae and intense production of keratin. In this work, the most important cellular events for the formation of the fetal hoof in Holstein cattle were first described in different stages of their formation.

A literatura científica carece de descrições morfológicas detalhadas sobre o desenvolvimento histológico e diferenciação celular do casco fetal bovino. Neste estudo, 40 extremidades de membros de fetos bovinos da raça Holandesa foram coletados e divididos em quatro grupos (G1 a G4) com base na estimativa da idade. Desses membros, foram retirados fragmentos da parede e sola, processados e corados por hematoxilina-eosina (HE) para observação em microscopia óptica. No grupo G1, observou-se que a epiderme era bastante delgada, incluindo camadas de queratinócitos e conjuntos de células mesenquimais. No grupo G2, foi observado que a espessura da epiderme, cobrindo as extremidades dos membros, permaneceu variável, e o cório laminar desenvolveu-se na camada germinativa. No grupo G3, notou-se, no epitélio germinal, papilas em desenvolvimento pouco avançado e células da camada córnea no processo inicial de queratinização. No grupo G4, a epiderme apresentava-se bem desenvolvida, com camadas distribuídas de forma homogênea, contendo papilas simétricas e longas e com intensa produção de queratina. Neste trabalho, os eventos celulares mais importantes para a formação do casco fetal em bovinos da raça Holandesa foram descritos pela primeira vez, em diferentes etapas da sua formação.

Microstructure of Holstein and Gir breed adult bovine hooves: histomorphometry, three-dimensional microtomography and microhardness test evaluation / Microestrutura de cascos de bovinos adultos das raças Holandesa e Gir: avaliação por histomorfometria, microtomografia tridimensional e teste de microdureza

This study aimed to characterize and compare the aspects involved in the microstructural formation of the Holstein and Gir breed hoof wall by histomorphometry, three-dimensional microtomography and microhardness test techniques. Seventy-two (18H/18G thoracic and 18H/18G pelvic) Holstein (H) and Gir (G) breed hooves were collected in slaughterhouses. The hooves were divided into six groups according to breed and age group: 24 to 36 months (C1H/C1G), 36 to 60 months (C2H/C2G) and over 60 months (C3H/C3G). The normality and homogeneity analysis of morphometric variables by the Kolmogrov-Sminov and Bartlett tests was conducted as a statistical model. Once the assumptions were met, the Sigmastat 3.5 software was used and the means were compared by T test. The 5% probability level is considered. When the assumptions were not met, the means were compared by the Mann-Whitney nonparametric test, considering the 5% probability level. When comparing the Holstein and Gir breeds, no differences were noticed between them as to the length of the dermal papillae; young animals showed thicker papillae than adult animals; the Holstein breed hooves showed higher amount of 7pores on the wall and on the sole compared to the Gir breed; Holstein cattle hooves showed greater microhardness than Gir cattle; there was no microhardness difference between pigmented and non-pigmented hooves of Holstein and Gir cattle.

O presente estudo objetivou caracterizar e comparar os aspectos envolvidos na formação microestrutural do estojo córneo de bovinos das raças Holandesa e Gir pelas técnicas de histomorfometria, microtomografia tridimensional e teste de microdureza. Foram coletados em frigoríficos, de forma igualitária entre as raças Holandesa (H) e Gir (G), 72 cascos (18H/18G torácicos e 18H/18G pélvicos). Os cascos foram divididos em seis grupos de acordo com a raça e a faixa etária: 24 a 36 meses, (C1H/C1G), 36 a 60 meses (C2H/C2G) e acima de 60 meses (C3H/C3G). Como modelo estatístico, realizou-se análise de normalidade e homogeneidade das variáveis morfométricas empregando testes Kolmogrov-Sminov e Bartlett. Atendidas as pressuposições, empregou-se o programa Sigmastat 3.5 e compararam-se as médias pelo teste T. Considerou-se o nível de 5% de probabilidade. Ao comparar as raças Holandesa e Gir, observou-se que não houve diferença entre elas quanto ao comprimento das papilas dérmicas. Os animais jovens apresentaram papilas mais espessas que os adultos. Os cascos da raça Holandesa apresentaram maior quantidade de poros na muralha e na sola em relação à raça Gir. Os cascos de bovinos Holandeses possuem microdureza maior que os de bovinos Gir. No estudo não se evidenciou diferença de microdureza entre cascos pigmentados e despigmentados de bovinos Holandeses e Gir.

Cyclic related and pathological changes in the lectin-binding sites on the swine oviduct.

This study was carried out to compare the lectin-binding pattern in the normal and pathological oviduct of sows during the ovarian cycle. Lectin-binding patterns showed differences between segments, phases of ovarian cycle and presence of morphologic lesions. In the infundibulum, it was observed that the cysts, in the follicular phase, reduced Ricinus communis-I (RCA-I) and Ulex europaeus-I (UEA-I) binding. Furthermore, in the pathological oviducts of the luteal-phase group, there is a reduction of Concanavalia ensiformis (Con-A) reactivity in this segment of the tube having wall cysts, adenomyosis and diverticulus. The Arachis hypogaea (PNA) binding in the infundibulum, during the luteal phase, decreased in the tube having adenomyosis. In animals with wall cysts, the Con-A, Triticum vulgaris (WGA) and RCA-I reactivity was minor in the glycocalyx of the isthmus epithelium during follicular phase. Con-A and Dolichos biflorus (DBA) binding pattern was minor in the luteal-phase isthmus of the tube having wall cysts, adenomyosis and diverticulus. In the ampulla, the wall cysts impaired the Con-A reaction only in the basal region of the epithelium, in the follicular phase. Binding with Con-A was decreased in the ampulla of animals in the luteal phase in the tube lesions with cysts and diverticulus. In addition, the diverticulus observed in the ampulla, during the luteal phase, reduced the PNA tubaric binding. The results of this study showed that the morphologic alterations modify the sugar pattern in the oviduct of sows. These modifications in glycoconjugates may be one of the reasons for the failure of fertility in sows.

Pilocytic astrocytoma in a cat.

A 12-year-old crossbred female cat was presented for necropsy with a history of anorexia, ataxia, convulsions, and blindness. Grossly, a whitish, firm, and well-circumscribed nodule with an irregular surface of approximately 1.5 cm in diameter was observed in the right dorsal, parietal, and occipital lobes of the brain. Histologically, the tumor was composed of neoplastic astrocytes forming irregular interweaving sheets and bundles. These cells were elongated, spindle-shaped, and bipolar, and some had clear cytoplasm and a dense nuclear chromatin pattern. Extensive areas of cartilaginous and osseous metaplasia were observed. By immunohistochemistry, the neoplastic cells were found to be markedly positive for S-100 protein and glial fibrillary acidic protein. On the basis of morphologic and immunohistochemical features, the diagnosis of pilocytic astrocytoma was made.

Leiomioma de vesícula biliar em cäo / Leiomyoma of the gall bladder in a dog

This report describes the pathological findings of a leiomyoma in the gall bladder of a female 11-year-old Doberman dog. The animal underwent euthanasia due to the poor prognosis of an osteosarcoma of the skull. Grossly, there was a firm smooth whitish nodule in the gall bladder with 0.5 centimeter in diameter. Histologically the nodule was composed of isomorphic and well differentiated leiomyocytes arranged in bundles. The dagnosis was confirmed by Van Gieson staining

Lipossarcoma em cäo: relato de dois casos / Canine liposarcoma: report of two cases

This work aims to describe the clinical and anatomopathological findings of two cases of liposarcoma diagnosed in the subcutaneous and abdominal cavity of dogs. In both cases, the tumoral masses were surgically removed and samples were submitted for histopathological analyses. Microscopic examination of each case revealed a highly cellular, pleomorphic neoplasm with round or polygonal fat cells. The nuclei was elongate or splindle-shape (subcutaneous liposarcoma) and round or ovoid (abdominal liposarcoma), contained margined chromatin, and prominent nucleolus. Cytoplasmic vacuoles that stained positively with Sudan IV varied highly in size

Inflamaçäo pseudotumoral do baço em cäo: relato de caso / Inflammatory pseudotumour of the spleen in dog: a case report

Descrevem-se pela primeira vez achados anátomo-histopatológicos da inflamaçäo pseudotumoral do baço, caracterizados pela presença de células inflamatórias, neutrófilos, linfócitos, plasmócitos, macrófagos e células gigantes multinucleadas, em uma cadela sem raça definida, de 10 anos de idade

Leiomiossarcoma pulmonar e renal em cäo: relato de caso / Pulmonary and renal leiomyosarcoma in dog: a case report

Este relato descreve um caso de leiomiossarcoma pulmonar e renal em cäo da raça Husky Siberiano, macho, de nove anos de idade e com histórico de emagrecimento progressivo há mais de três meses. Ao exame clínico, o animal apresentava palidez discreta das mucosas e dor intensa à palpaçäo abdominal, além de aumento de volume acentuado dos rins. No hemograma, foi evidenciada anemia discreta e na urinálise foram observados isostenúria e grumos de células do epitélio renal. Os níveis plasmáticos de uréia e creatinina estavam dentro dos limites considerados normais. O animal foi sacrificado após a confirmaçäo de neoplasia renal bilateral por laparotomia exploratória. A necropsia, os rins apresentavam vários nódulos de 0,5 a 4cm de diâmetro, esbranquiçados e firmes. Na regiao do hilo e no lobo apical esquerdo dos pulmöes havia aumento de volume, com 11 x 7cm de extensäo, de superfície irregular e bem vascularizada, esbranquiçada e com áreas de consistência ora firmes, ora friáveis. No lobo apical direito também havia um nódulo com 1cm de diâmetro, firme e esbranquiçado. Secçöes histológicas dos pulmöes e dos rins foram coradas pela hematoxilina-eosina, Masson e Van Gieson. Foi detectada positividade, pela imuno-histoquímica, para a vimentina e actina alfa de músculo liso. Nao houve imunomarcaçäo para citoqueratinas 1, 5, 10, 14, 8 e 7, proteína S100 e para CD68. Com base nos achados anatomopatológicos e imuno-histoquímicos foi firmado o diagnóstico de leiomiossarcoma pulmonar e renal, sem, no entanto, definir o sítio primário da neoplasia

Mixoma de vulva em cäo / Vulvar myxoma in a dog

This report describes the pathological findings of a myxoma in the vulva of a seven-year-old German Shepherd bitch, with history of mass increase in the vulva. After surgery, tipical and microscopic aspects were detected

Atividade secretória de tubas uterinas com e sem patologia de porcas adultas nas fases folicular e luteínica / Secretory activity of uterine tubes with and without alterations of sexually mature sows in the folicular and luteal phases

Descrevem-se as características anatomopatológicas e histoquímicas de 124 tubas uterinas de porcas (matrizes) abatidas em matadouros, nas fases folicular e luteínica do ciclo estral. As tubas uterinas foram distribuídas em quatro grupos: LSP- fase luteínica sem patologia tubárica (n=45), LCP- fase luteínica com patologia tubárica (n=19), FSP- fase folicular sem patologia tubárica (n=45) e FCP- fase folicular com patologia tubárica (n=15). Para estudo histoquímico de mucossubstâncias foram utilizadas as colorações de periodic acid Schiff (PAS) com e sem digestão prévia pela amilase salivar e Alcian Blue (AB) em pH 2,5 e 0,4. Utilizou-se a coloração de Ninhidrina-Schiff para evidenciação de proteínas ricas em radicais -NH2. Foram observadas alterações em 34 tubas uterinas. Independente da fase do ciclo estral, as patologias mais comumente encontradas foram os cistos de parede e a metaplasia escamosa. Em menor número foram observados divertículo e adenomiose. A ampola e o infundíbulo foram os segmentos mais afetados. Na fase folicular, o infundíbulo apresentou maior intensidade de secreção de mucossubstâncias neutras e AB 2,5-positivas. Na ampola houve maior intensidade de secreção de mucossubstâncias AB 2,5-positivas, sulfomucinas e de proteínas ricas em radicais -NH2. As patologias não reduziram significativamente a secreção de mucossubstâncias nas regiões adjacentes em nenhum dos segmentos tubáricos, tanto na fase luteínica quanto na folicular. Nas áreas de metaplasia escamosa do epitélio tubárico e nos cistos de parede houve redução da secreção de mucossubstâncias neutras e ácidas.

Mesotelioma peritoneal em cäo: relato de caso / Peritoneal mesothelioma in dog: a case report

Este estudo descreve os achados anatomopatológicos e imuno-histoquímicos do mesotelioma peritoneal em um cäo de raça Setter, de nove anos de idade e sem histórico clínico. A necropsia, foram observados vários nódulos que variavam de alguns milímetros a 2cm de diâmetro, de superfície lisa, esbranquiçados, firmes e distribuídos amplamente pelos peritônios parietal e visceral dos órgaos abdominais e pelo funículo espermático. Histologicamente foram evidenciados ninhos de células mesoteliais neoplásicas envoltos por grande quantidade de tecido conjuntivo fibroso. Essas células apresentavam-se fusiformes ou epitelióides com citoplasma vacuolizado e núcleo oval com cromatina frouxa e nucléolo evidente. Havia também algumas formaçöes císticas revestidas por uma ou mais camadas de células neoplásicas achatadas ou colunares e com o lúmen ocupado por debris celulares e material eosinofílico, PAS-positivo. Areas de metaplasia cartilaginosa e de necrose também forma visualizadas. O resultado das reaçöes imumo-histoquímicas revelou a presença de células neoplásicas com imunomarcaçöes fortes e difusas para proteína S100 e para citoqueratina (AE/AE3) e ausência de marcaçäo para vimentina e para o antígeno carcinoembrionário (CEA). A imunomarcaçäo das células neoplásicas com a utilizaçäo do anticorpo contra célula mesotelial humana (HBME-1) foi forte e multifocal. Com base nos achados anatomopatalógicos e imuno-histoquímico, firmou-se o diagnóstico de mesotelioma peritoneal esclerosante

Altura do epitélio da tuba uterina normal e patológica de porcas nas fases folicular e luteínica / Epithelium of the uterine tube of sows in the folicular and lutheal phases

The aim of this work was to perform a morphometric analysis of the uterine tube epithelium of sexually mature sows, with or without pathological changes. The sows were either in follicular or luteal phases. One hundred and twenty-four uterine tubes of sixty-two sows were obtained from slaughterhouses. Sections of the uterine tube, uterus and ovary were processed for morphometrical evaluation. The uterine tubes were divided into four groups. The first (LSP) group was composed by animals in luteal phase without pathological changes (n=45), the second (LCP) by animals in luteal phase with pathological changes (n=19), the third (FSP) by animals in follicular phase without pathological changes (n=45) and the fourth (FCP) by animals in follicular phase with pathological changes (n=15). In the follicular phase (FSP), the ampulla had a higher epithelium, followed by the infundibulum and the isthmus (P<0.05). Tubal epithelium was higher in the animals of FSP and FCP groups when compared to LSP and LCP, suggesting that estrogens influence epithelial height. In the isthmus, there were significant differences between FSP and LSP groups (P<0.05). These results indicate that the porcine uterine tube epithelium changes its height during the estrous cycle. The epithelial height does not appear to be influenced by tubarian pathologies