RESUMEN
This research paper aims to investigate if oxidative stress biomarkers increase after a painful procedure in term newborns and if nonpharmacological approaches, or sex, influence pain degree, and the subsequent OS. 83 healthy term newborns were enrolled to receive 10% oral glucose or sensorial saturation (SS) for analgesia during heel prick (HP). The ABC scale was used to score the pain. Advanced oxidation protein products (AOPP) and total hydroperoxides (TH) as biomarkers of OS were measured at the beginning (early-sample) and at the end (late-sample) of HP. The early-sample/late-sample ratio for AOPP and TH was used to evaluate the increase in OS biomarkers after HP. Higher levels of both AOPP and TH ratio were observed in high degree pain (4-6) compared with low degree pain score (0-3) (AOPP: p = 0.049; TH: p = 0.001). Newborns receiving SS showed a significantly lower pain score (p = 0.000) and AOPP ratio levels (p = 0.021) than those without. Males showed higher TH levels at the end of HP (p = 0.005) compared to females. The current study demonstrates that a relationship between pain degree and OS exists in healthy full-term newborns. The amount of OS is gender related, being higher in males. SS reduces pain score together with pain-related OS in the newborns.
Asunto(s)
Estrés Oxidativo/fisiología , Dolor/fisiopatología , Productos Avanzados de Oxidación de Proteínas/sangre , Femenino , Humanos , Peróxido de Hidrógeno/sangre , Recién Nacido , MasculinoRESUMEN
OBJECTIVES: To test the hypothesis that neonatal supplementation with lutein in the first hours of life reduces neonatal oxidative stress (OS) in the immediate postpartum period. METHODS: A randomized controlled, double-blinded clinical trial was conducted among 150 newborns divided into control group, not supplemented (n = 47), and test group, supplemented with lutein on the first day postpartum (n = 103). Blood Samples were collected at birth from cord and at 48 hrs postpartum while routine neonatal metabolic screenings were taking place. Total hydroperoxide (TH), advanced oxidation protein products (AOPP), and biological antioxidant potential (BAP) were measured by spectrophotometry and data were analyzed by Wilcoxon rank sum test and by multivariate logistic regression analysis. RESULTS: Before lutein supplementation, the mean blood concentrations of AOPP, TH, and BAP were 36.10 umol/L, 156.75 mmol/H2O2, and 2361.04 umol/L in the test group. After lutein supplementation, significantly higher BAP increment (0.17 ± 0.22 versus 0.06 versus ± 0.46) and lower TH increment (0.46 ± 0.54 versus 0.34 ± 0.52) were observed in the test group compared to controls. CONCLUSION: Neonatal supplementation with lutein in the first hours of life increases BAP and reduces TH in supplemented babies compared to those untreated. The generation of free radical-induced damage at birth is reduced by lutein. This trial is registered with ClinicalTrials.gov NCT02068807.
Asunto(s)
Luteína/farmacología , Estrés Oxidativo/efectos de los fármacos , Productos Avanzados de Oxidación de Proteínas/sangre , Antioxidantes/análisis , Antioxidantes/química , Antioxidantes/metabolismo , Área Bajo la Curva , Suplementos Dietéticos , Método Doble Ciego , Femenino , Humanos , Peróxido de Hidrógeno/sangre , Recién Nacido , Peroxidación de Lípido/efectos de los fármacos , Modelos Logísticos , Masculino , Curva ROC , EspectrofotometríaRESUMEN
In this research we studied the possible relationships between the level and the distribution of peroxidase-catalase activity and the degree of the morphological changes in the intestinal wall during mechanical occlusion. These researches have really proved that the increase of the peroxidase-catalase enzymes correlate proportionally with the damage of intestinal wall.
Asunto(s)
Enfermedades del Íleon/patología , Obstrucción Intestinal/patología , Animales , Femenino , Mucosa Intestinal/enzimología , Mucosa Intestinal/patología , Masculino , Peroxidasa/análisis , RatasRESUMEN
In this research we studied the interrelationships between the progression of morphological damage and leucocyte populations in the last ileal loop after blockage of blood supply subsequent to arterial and venous occlusion. Our morphological data on the staging of the ileal wall damage agree with those reported in literature. In addition we described that in the ischemic loop the number of lymphocytes appear decreased and polymorphonuclear cells degranulate actively.
Asunto(s)
Íleon/irrigación sanguínea , Isquemia/patología , Animales , Íleon/patología , Inflamación , Linfocitos/patología , Neutrófilos/patología , Ratas , Ratas EndogámicasRESUMEN
Superoxide radicals produced during acute intestinal ischemia are biochemically related with the presence of hydrogen peroxyde. In this study we have investigated the distribution of peroxidase-catalase activity, histochemically determined, in the ischemic ileal wall. In the rat, complete arterial and venous occlusion produced a progressive increase in extra-vascular peroxidase-catalase activity with a maximum corresponding to the ileal wall. Probably the tissue peroxidase-catalase activity is related to massive degranulation of polymorphonucleates.